Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial translation initiation factor 2 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 0 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 0 | – | – | – | 1 | 2 | 2 | 0 | 3 | 9 |
Bud | 4 | 4 | 1 | – | 0 | 0 | 1 | 5 | 2 | 6 | 6 | 0 | 2 | 2 | 0 | – | – | – | 0 | 0 | 0 | 1 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 1 |
Bud Site | 2 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 5 | 4 | 1 | – | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 8 | 3 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 3 | 11 | 2 | – | 3 | 3 | 3 | 6 | 7 | 2 | 5 | 3 | 23 | 28 | 23 | – | – | – | 0 | 0 | 2 | 3 | 2 | 5 |
Endoplasmic Reticulum | 16 | 2 | 0 | – | 0 | 0 | 3 | 4 | 2 | 2 | 2 | 3 | 0 | 2 | 5 | – | – | – | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 4 | 11 | 5 | – | 3 | 2 | 4 | 8 | 5 | 3 | 0 | 0 | 21 | 14 | 25 | – | – | – | 16 | 12 | 9 | 7 | 11 | 10 |
Golgi | 5 | 7 | 2 | – | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 6 | 4 | 4 | – | – | – | 8 | 13 | 2 | 6 | 9 | 13 |
Mitochondria | 181 | 135 | 26 | – | 10 | 50 | 47 | 116 | 44 | 71 | 71 | 46 | 51 | 44 | 37 | – | – | – | 164 | 120 | 51 | 67 | 106 | 148 |
Nucleus | 1 | 1 | 0 | – | 0 | 0 | 3 | 9 | 4 | 0 | 1 | 0 | 0 | 2 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 2 |
Nuclear Periphery | 6 | 1 | 0 | – | 0 | 0 | 1 | 2 | 1 | 1 | 2 | 3 | 0 | 0 | 1 | – | – | – | 2 | 1 | 1 | 0 | 1 | 1 |
Nucleolus | 2 | 2 | 0 | – | 1 | 3 | 3 | 1 | 0 | 2 | 2 | 2 | 1 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 1 | 0 |
Peroxisomes | 3 | 11 | 6 | – | 2 | 1 | 1 | 3 | 0 | 4 | 0 | 0 | 9 | 44 | 22 | – | – | – | 15 | 7 | 3 | 3 | 6 | 5 |
SpindlePole | 3 | 2 | 1 | – | 1 | 3 | 6 | 14 | 3 | 7 | 3 | 3 | 2 | 10 | 5 | – | – | – | 2 | 1 | 1 | 2 | 2 | 1 |
Vac/Vac Membrane | 28 | 16 | 7 | – | 2 | 5 | 7 | 9 | 5 | 5 | 2 | 0 | 28 | 15 | 9 | – | – | – | 7 | 5 | 3 | 3 | 10 | 13 |
Unique Cell Count | 206 | 158 | 42 | 19 | 58 | 67 | 149 | 60 | 84 | 80 | 49 | 117 | 142 | 109 | 224 | 172 | 85 | 99 | 161 | 222 | ||||
Labelled Cell Count | 265 | 207 | 53 | 24 | 68 | 82 | 178 | 75 | 104 | 94 | 61 | 145 | 180 | 134 | 224 | 172 | 85 | 99 | 161 | 222 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 5.6 | 6.1 | – | 7.5 | 5.3 | 5.4 | 5.8 | 5.4 | 4.8 | 4.9 | 4.3 | 7.5 | 7.4 | 6.7 | – | – | – | 8.1 | 6.8 | 7.4 |
Std Deviation (1e-4) | 0.7 | 1.2 | 1.3 | – | 2.0 | 1.5 | 1.2 | 1.5 | 1.5 | 1.1 | 1.6 | 0.9 | 3.3 | 3.6 | 2.4 | – | – | – | 4.5 | 2.2 | 2.9 |
Intensity Change (Log2) | – | – | – | – | 0.29 | -0.22 | -0.17 | -0.07 | -0.18 | -0.35 | -0.31 | -0.5 | 0.3 | 0.27 | 0.14 | – | – | – | 0.41 | 0.15 | 0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | – | – | – |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | – | – | – |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | – | – | – |
Cell Periphery | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | – | – | – |
Endosome | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0.9 | 0 | 1.5 | – | – | – |
Golgi | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | – | – | – |
Mitochondria | – | -0.7 | 2.8 | 0.9 | 2.1 | 1.2 | – | 3.5 | – | -2.0 | -3.6 | -3.1 | – | – | – |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | – | – | – |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 2.1 | 0.8 | – | – | – |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | – | – | – |
Cytoplasm | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 2.3 | 2.3 | 2.4 | – | – | – |
Nucleus | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | – | – | – |
Vacuole | – | 0 | -1.2 | -0.9 | 0 | 0 | – | 0 | – | 1.0 | -1.1 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.947 | 0.1971 | 0.4112 | 0.7073 | 0.1733 | 0.36 | 0.1002 | 0.7308 | 0.7167 | 0.7722 | -0.3326 | 0.5379 | 0.6552 | 0.9145 | 0.7853 | 1.2879 | 1.215 | 0.6135 |
Actin | 0.0269 | 0.0374 | 0.0375 | 0.0311 | 0.0072 | 0.0188 | 0.0521 | 0.0201 | 0.0351 | 0.012 | 0.0046 | 0.0299 | 0.0191 | 0.0146 | 0.0276 | 0.0515 | 0.0525 | 0.0076 |
Bud | 0.0011 | 0.0031 | 0.0069 | 0.0021 | 0.0013 | 0.0009 | 0.0014 | 0.0076 | 0.0019 | 0.0002 | 0.0006 | 0.01 | 0.0011 | 0.0036 | 0.0012 | 0.0072 | 0.004 | 0.0006 |
Bud Neck | 0.0016 | 0.0045 | 0.0077 | 0.004 | 0.0006 | 0.018 | 0.0049 | 0.0012 | 0.004 | 0.0002 | 0.0038 | 0.0262 | 0.001 | 0.0021 | 0.0018 | 0.0014 | 0.0028 | 0.0015 |
Bud Periphery | 0.0014 | 0.0058 | 0.0126 | 0.0042 | 0.0028 | 0.0022 | 0.0027 | 0.0137 | 0.0042 | 0.0004 | 0.0013 | 0.0109 | 0.0059 | 0.0047 | 0.0016 | 0.0056 | 0.0088 | 0.0009 |
Bud Site | 0.0074 | 0.0332 | 0.0389 | 0.0032 | 0.0025 | 0.0012 | 0.0155 | 0.0293 | 0.022 | 0.0008 | 0.0017 | 0.0039 | 0.0048 | 0.0361 | 0.007 | 0.0028 | 0.0086 | 0.0011 |
Cell Periphery | 0.0005 | 0.0018 | 0.0014 | 0.0005 | 0.0002 | 0.0007 | 0.0008 | 0.0009 | 0.0035 | 0.0002 | 0.0003 | 0.0012 | 0.0059 | 0.0018 | 0.0009 | 0.0003 | 0.0007 | 0.0002 |
Cytoplasm | 0.0054 | 0.0226 | 0.0072 | 0.0047 | 0.0014 | 0.0226 | 0.0397 | 0.02 | 0.0125 | 0.0003 | 0.0021 | 0.0162 | 0.0046 | 0.0268 | 0.0126 | 0.0062 | 0.0204 | 0.0071 |
Cytoplasmic Foci | 0.1294 | 0.1034 | 0.0911 | 0.058 | 0.0352 | 0.0774 | 0.0775 | 0.099 | 0.0469 | 0.0224 | 0.0342 | 0.0616 | 0.0735 | 0.099 | 0.0548 | 0.0678 | 0.1029 | 0.0411 |
Eisosomes | 0.001 | 0.0019 | 0.0006 | 0.0005 | 0.0002 | 0.0005 | 0.0009 | 0.0005 | 0.0009 | 0.0002 | 0.0001 | 0.0005 | 0.0014 | 0.0007 | 0.0011 | 0.0004 | 0.0003 | 0.0002 |
Endoplasmic Reticulum | 0.0041 | 0.0046 | 0.0012 | 0.0016 | 0.0004 | 0.0042 | 0.0023 | 0.0029 | 0.0011 | 0 | 0.0001 | 0.0012 | 0.0036 | 0.0039 | 0.0018 | 0.001 | 0.0039 | 0.001 |
Endosome | 0.2983 | 0.1385 | 0.0817 | 0.1348 | 0.0985 | 0.1419 | 0.0881 | 0.0732 | 0.0561 | 0.0092 | 0.0252 | 0.0443 | 0.1226 | 0.1536 | 0.1106 | 0.0957 | 0.1211 | 0.0946 |
Golgi | 0.1301 | 0.0751 | 0.0642 | 0.076 | 0.1173 | 0.0957 | 0.0533 | 0.0552 | 0.0407 | 0.0117 | 0.0209 | 0.0354 | 0.0836 | 0.1064 | 0.0955 | 0.0888 | 0.1112 | 0.0881 |
Lipid Particles | 0.0856 | 0.0387 | 0.0571 | 0.0445 | 0.0119 | 0.043 | 0.0607 | 0.0253 | 0.0168 | 0.0036 | 0.0028 | 0.0452 | 0.0629 | 0.0237 | 0.018 | 0.0043 | 0.0098 | 0.0152 |
Mitochondria | 0.1747 | 0.3894 | 0.4906 | 0.5643 | 0.6187 | 0.4544 | 0.3889 | 0.4692 | 0.5949 | 0.8116 | 0.7073 | 0.5321 | 0.5156 | 0.4197 | 0.5959 | 0.6034 | 0.4249 | 0.6685 |
None | 0.0151 | 0.0165 | 0.035 | 0.0021 | 0.0004 | 0.0326 | 0.0357 | 0.0612 | 0.0326 | 0.0002 | 0.0003 | 0.0466 | 0.0079 | 0.0143 | 0.0182 | 0.0111 | 0.0013 | 0.0006 |
Nuclear Periphery | 0.0118 | 0.0023 | 0.0006 | 0.001 | 0.0001 | 0.002 | 0.0069 | 0.0013 | 0.0016 | 0 | 0 | 0.0012 | 0.0062 | 0.001 | 0.0008 | 0.0008 | 0.0066 | 0.0059 |
Nucleolus | 0.0019 | 0.0048 | 0.0014 | 0.0005 | 0.0002 | 0.0006 | 0.001 | 0.002 | 0.0015 | 0.0001 | 0.0002 | 0.0005 | 0.0006 | 0.0008 | 0.0004 | 0.0018 | 0.0003 | 0.0001 |
Nucleus | 0.0016 | 0.0011 | 0.0008 | 0.0007 | 0.0003 | 0.0016 | 0.0019 | 0.0031 | 0.0009 | 0.0001 | 0.0002 | 0.0008 | 0.0015 | 0.0009 | 0.0007 | 0.0017 | 0.001 | 0.0003 |
Peroxisomes | 0.0668 | 0.0887 | 0.0505 | 0.0497 | 0.0945 | 0.0724 | 0.1419 | 0.1023 | 0.1075 | 0.1256 | 0.1909 | 0.1142 | 0.0651 | 0.0669 | 0.035 | 0.0402 | 0.0826 | 0.0568 |
Punctate Nuclear | 0.0219 | 0.0005 | 0.001 | 0.0016 | 0.0002 | 0.0016 | 0.0097 | 0.003 | 0.0008 | 0.0002 | 0.0001 | 0.0008 | 0.0006 | 0.0008 | 0.0008 | 0.0016 | 0.0064 | 0.0002 |
Vacuole | 0.008 | 0.0193 | 0.007 | 0.0074 | 0.0029 | 0.0046 | 0.0094 | 0.0066 | 0.0097 | 0.0005 | 0.0025 | 0.0061 | 0.0069 | 0.0134 | 0.0084 | 0.0031 | 0.008 | 0.0034 |
Vacuole Periphery | 0.0055 | 0.0066 | 0.005 | 0.0074 | 0.0031 | 0.0032 | 0.0047 | 0.0024 | 0.0049 | 0.0005 | 0.0008 | 0.0113 | 0.0056 | 0.0052 | 0.0054 | 0.0031 | 0.0219 | 0.0048 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.9761 | 8.2562 | 5.7661 | 7.8094 | 7.3961 | 10.8596 | 9.6983 | 11.1028 | 8.2176 | 10.8313 |
Translational Efficiency | 0.7516 | 0.6048 | 0.6073 | 0.5412 | 0.4758 | 0.4545 | 0.7202 | 0.5025 | 0.7444 | 0.6848 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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