Standard name | |
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Human Ortholog | |
Description | Mitochondrial glutamyl-tRNA synthetase; predicted to be palmitoylated |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 1 | 1 | 4 | 5 | 1 | 4 | 7 |
Bud | 2 | 1 | 12 | 12 | 12 | 7 | 25 | 26 | 8 | 24 | 0 | 1 | 2 | 2 | 1 | 7 | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 1 |
Bud Site | 3 | 0 | 1 | 2 | 0 | 3 | 7 | 8 | 7 | 9 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 4 | 14 | 5 | 3 | 0 | 2 | 1 | 1 | 0 | 2 | 0 | 1 | 6 | 5 | 6 | 19 | 7 | 10 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 1 | 10 | 5 | 11 | 12 | 19 | 23 | 11 | 22 | 5 | 5 | 6 | 7 | 12 | 37 | 30 | 16 | 0 | 0 | 1 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 11 | 9 | 5 | 3 | 4 | 3 | 3 | 5 | 3 | 0 | 0 | 7 | 2 | 10 | 14 | 6 | 8 | 1 | 0 | 0 | 0 | 2 | 2 |
Endosome | 19 | 1 | 13 | 3 | 9 | 1 | 6 | 4 | 1 | 0 | 0 | 0 | 3 | 4 | 16 | 45 | 37 | 20 | 20 | 14 | 19 | 6 | 7 | 10 |
Golgi | 0 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 5 | 21 | 11 | 14 | 9 | 10 | 14 | 7 | 5 | 8 | 9 |
Mitochondria | 212 | 215 | 196 | 167 | 221 | 210 | 386 | 410 | 302 | 378 | 26 | 41 | 93 | 147 | 119 | 24 | 20 | 18 | 197 | 193 | 185 | 82 | 113 | 94 |
Nucleus | 3 | 0 | 1 | 1 | 1 | 4 | 8 | 12 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 6 | 4 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 2 | 6 | 2 | 1 | 2 | 4 | 12 | 12 | 7 | 12 | 0 | 0 | 1 | 1 | 1 | 3 | 2 | 0 | 3 | 1 | 2 | 0 | 0 | 1 |
Nucleolus | 8 | 0 | 0 | 1 | 1 | 2 | 6 | 15 | 2 | 6 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 2 |
Peroxisomes | 21 | 6 | 18 | 4 | 7 | 2 | 8 | 6 | 3 | 0 | 0 | 0 | 5 | 13 | 11 | 29 | 19 | 16 | 7 | 9 | 23 | 8 | 12 | 11 |
SpindlePole | 3 | 0 | 17 | 3 | 13 | 8 | 28 | 33 | 5 | 13 | 1 | 2 | 0 | 1 | 2 | 26 | 21 | 9 | 1 | 0 | 1 | 2 | 2 | 2 |
Vac/Vac Membrane | 23 | 28 | 11 | 4 | 16 | 6 | 18 | 10 | 8 | 6 | 0 | 1 | 8 | 15 | 11 | 35 | 36 | 15 | 4 | 9 | 3 | 3 | 4 | 2 |
Unique Cell Count | 242 | 228 | 227 | 180 | 253 | 227 | 422 | 458 | 315 | 402 | 31 | 45 | 116 | 171 | 168 | 218 | 166 | 112 | 255 | 255 | 256 | 112 | 162 | 155 |
Labelled Cell Count | 304 | 289 | 302 | 211 | 297 | 265 | 528 | 565 | 365 | 483 | 33 | 52 | 149 | 204 | 211 | 260 | 205 | 130 | 255 | 255 | 256 | 112 | 162 | 155 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 5.0 | 4.9 | 4.3 | 4.4 | 4.0 | 3.7 | 4.0 | 3.6 | 3.6 | 4.2 | 3.5 | 5.9 | 5.7 | 5.7 | 5.6 | 6.5 | 7.0 | 4.8 | 5.2 | 6.5 |
Std Deviation (1e-4) | 1.2 | 0.8 | 1.2 | 1.0 | 1.1 | 1.2 | 1.3 | 1.3 | 0.9 | 1.3 | 1.7 | 1.1 | 1.1 | 1.6 | 1.2 | 1.4 | 1.9 | 1.9 | 1.0 | 0.9 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.18 | -0.15 | -0.29 | -0.41 | -0.28 | -0.43 | -0.46 | -0.21 | -0.5 | 0.27 | 0.22 | 0.22 | 0.2 | 0.4 | 0.51 | -0.02 | 0.1 | 0.41 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | -2.2 | -2.6 | -1.1 | -0.8 | -0.3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 3.0 | 1.1 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0.9 | 0 | 0.9 | 1.2 | -0.2 | 1.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | -1.3 | -1.7 | 1.4 | 4.7 | 4.9 | 3.5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 5.1 | 0 | 4.9 | 2.6 | 3.7 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | -1.5 | -0.1 | -3.8 | -15.9 | -14.6 | -12.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | -1.3 | -0.1 | -0.5 | 1.8 | 1.2 | 1.8 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | -3.0 | -3.3 | -2.9 | 1.6 | 1.7 | 0.2 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.9 | 0 | 0.4 | 0.1 | 0.4 | -0.5 | 0.6 | – | – | 0.3 | -0.2 | 1.2 | 4.3 | 4.4 | 3.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.4 | 0.7 | -1.2 | -0.3 | -1.9 | -1.4 | -2.5 | – | – | 0.8 | 1.6 | 0.7 | 3.9 | 5.1 | 2.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7201 | 1.873 | 1.7628 | 1.656 | 1.6578 | 1.7154 | 2.0532 | 2.3848 | 2.2021 | 2.0251 | 1.6629 | 2.405 | 2.9098 | 2.5196 | 2.5593 | 3.5282 | 2.9461 | 2.3059 |
Actin | 0.0334 | 0.0166 | 0.0166 | 0.0425 | 0.0162 | 0.0125 | 0.0978 | 0.022 | 0.0641 | 0.0451 | 0.0131 | 0.0613 | 0.0344 | 0.0358 | 0.0243 | 0.0839 | 0.0183 | 0.0197 |
Bud | 0.0029 | 0.0041 | 0.0033 | 0.0026 | 0.006 | 0.0009 | 0.0021 | 0.0023 | 0.0043 | 0.0049 | 0.0019 | 0.0041 | 0.0006 | 0.0031 | 0.0017 | 0.0083 | 0.0007 | 0.0009 |
Bud Neck | 0.0046 | 0.0016 | 0.0195 | 0.0059 | 0.0018 | 0.0101 | 0.0028 | 0.0026 | 0.0098 | 0.0023 | 0.0018 | 0.0089 | 0.0011 | 0.0026 | 0.0041 | 0.0041 | 0.0008 | 0.0057 |
Bud Periphery | 0.0045 | 0.0092 | 0.0093 | 0.0132 | 0.0335 | 0.0047 | 0.0038 | 0.0042 | 0.0131 | 0.0169 | 0.0037 | 0.0059 | 0.002 | 0.0049 | 0.0059 | 0.0245 | 0.0012 | 0.003 |
Bud Site | 0.0239 | 0.064 | 0.0214 | 0.0281 | 0.0068 | 0.0021 | 0.0171 | 0.0332 | 0.0277 | 0.0137 | 0.0026 | 0.0039 | 0.0024 | 0.0536 | 0.0228 | 0.0067 | 0.0009 | 0.0028 |
Cell Periphery | 0.0038 | 0.0095 | 0.018 | 0.023 | 0.007 | 0.0027 | 0.0013 | 0.0014 | 0.0117 | 0.0025 | 0.0004 | 0.0028 | 0.0022 | 0.0025 | 0.0063 | 0.0013 | 0.0004 | 0.004 |
Cytoplasm | 0.0451 | 0.0467 | 0.0388 | 0.0488 | 0.0102 | 0.0206 | 0.0475 | 0.0237 | 0.027 | 0.0368 | 0.007 | 0.0213 | 0.0161 | 0.012 | 0.0215 | 0.0075 | 0.0238 | 0.033 |
Cytoplasmic Foci | 0.1458 | 0.0286 | 0.0468 | 0.0464 | 0.0476 | 0.0575 | 0.1142 | 0.1105 | 0.0786 | 0.0747 | 0.0675 | 0.0768 | 0.1099 | 0.0783 | 0.0614 | 0.0331 | 0.0734 | 0.0463 |
Eisosomes | 0.0009 | 0.0025 | 0.0043 | 0.0051 | 0.0008 | 0.0006 | 0.0023 | 0.0013 | 0.0076 | 0.0007 | 0.0004 | 0.0012 | 0.0012 | 0.0014 | 0.0037 | 0.0026 | 0.003 | 0.0027 |
Endoplasmic Reticulum | 0.0112 | 0.0092 | 0.0066 | 0.0038 | 0.0035 | 0.0023 | 0.0098 | 0.0012 | 0.0042 | 0.0024 | 0.001 | 0.0021 | 0.0036 | 0.0044 | 0.0021 | 0.0009 | 0.0036 | 0.0034 |
Endosome | 0.1297 | 0.0505 | 0.0777 | 0.0905 | 0.0678 | 0.0673 | 0.1 | 0.0825 | 0.0723 | 0.1021 | 0.0859 | 0.0624 | 0.1367 | 0.0961 | 0.0462 | 0.0921 | 0.165 | 0.0618 |
Golgi | 0.1111 | 0.0436 | 0.042 | 0.0477 | 0.0562 | 0.0517 | 0.0563 | 0.0756 | 0.0503 | 0.058 | 0.0812 | 0.0523 | 0.0792 | 0.0708 | 0.0325 | 0.0154 | 0.0881 | 0.0576 |
Lipid Particles | 0.1217 | 0.0314 | 0.0245 | 0.0107 | 0.0449 | 0.0149 | 0.053 | 0.0249 | 0.0338 | 0.0426 | 0.0241 | 0.0205 | 0.0452 | 0.0226 | 0.0345 | 0.0016 | 0.0326 | 0.0301 |
Mitochondria | 0.1522 | 0.577 | 0.5386 | 0.5255 | 0.5856 | 0.6526 | 0.2142 | 0.4809 | 0.4011 | 0.4371 | 0.4987 | 0.5477 | 0.4063 | 0.4809 | 0.6093 | 0.5723 | 0.4085 | 0.5955 |
None | 0.0765 | 0.0522 | 0.0567 | 0.0729 | 0.005 | 0.0653 | 0.1239 | 0.0467 | 0.0848 | 0.0397 | 0.0608 | 0.0587 | 0.0406 | 0.0721 | 0.0644 | 0.1155 | 0.1268 | 0.0591 |
Nuclear Periphery | 0.0065 | 0.0023 | 0.0013 | 0.0004 | 0.0011 | 0.0006 | 0.0131 | 0.0004 | 0.0035 | 0.0013 | 0.0012 | 0.0007 | 0.002 | 0.004 | 0.0004 | 0.0005 | 0.0008 | 0.0006 |
Nucleolus | 0.0161 | 0.0008 | 0.0023 | 0.0007 | 0.0012 | 0.0006 | 0.0029 | 0.001 | 0.0014 | 0.0004 | 0.0004 | 0.001 | 0.0011 | 0.0015 | 0.0013 | 0.0002 | 0.0005 | 0.0004 |
Nucleus | 0.0085 | 0.0012 | 0.0017 | 0.0008 | 0.0016 | 0.0006 | 0.0084 | 0.0007 | 0.0015 | 0.0011 | 0.0006 | 0.0014 | 0.0008 | 0.0016 | 0.0009 | 0.0003 | 0.0006 | 0.0004 |
Peroxisomes | 0.0683 | 0.0295 | 0.0521 | 0.0164 | 0.0843 | 0.0216 | 0.1025 | 0.0772 | 0.0864 | 0.1042 | 0.1387 | 0.0591 | 0.0908 | 0.0365 | 0.0395 | 0.008 | 0.0418 | 0.0651 |
Punctate Nuclear | 0.0051 | 0.0006 | 0.0008 | 0.0002 | 0.001 | 0.0006 | 0.0155 | 0.0007 | 0.0009 | 0.0012 | 0.0009 | 0.0017 | 0.0053 | 0.0006 | 0.001 | 0.0007 | 0.0013 | 0.0013 |
Vacuole | 0.0234 | 0.014 | 0.0131 | 0.0113 | 0.0122 | 0.0061 | 0.0086 | 0.0055 | 0.0096 | 0.0071 | 0.0041 | 0.0034 | 0.0103 | 0.0109 | 0.0125 | 0.0102 | 0.0046 | 0.0035 |
Vacuole Periphery | 0.0047 | 0.005 | 0.0046 | 0.0036 | 0.0057 | 0.004 | 0.003 | 0.0018 | 0.0063 | 0.0053 | 0.004 | 0.0026 | 0.0084 | 0.0038 | 0.0037 | 0.0103 | 0.0031 | 0.0031 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.2738 | 6.1228 | 5.452 | 8.584 | 8.8803 | 8.2145 | 7.7384 | 10.8636 | 10.1419 | 12.3078 |
Translational Efficiency | 0.4772 | 0.4627 | 0.7626 | 0.5793 | 0.5408 | 0.8039 | 0.6633 | 0.5804 | 0.7214 | 0.5558 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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