Standard name
Human Ortholog
Description Mitochondrial glutamyl-tRNA synthetase; predicted to be palmitoylated

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0.05 0.07 0.05 0 0.06 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.06 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.09 0 0.09 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0.05 0.05 0 0.05 0.16 0.11 0.05 0 0.07 0.17 0.18 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0.06 0 0.07 0 0 0 0 0 0
Endosome 0.08 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.1 0.21 0.22 0.18 0.08 0.06 0.08 0.05 0.05 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0 0.12 0.05 0.08 0.08 0 0.06 0 0 0.05 0.06
Mitochondria 0.88 0.94 0.86 0.93 0.87 0.93 0.91 0.9 0.96 0.94 0.84 0.91 0.8 0.86 0.71 0.11 0.12 0.16 0.77 0.76 0.73 0.74 0.7 0.61
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.09 0 0.08 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.13 0.11 0.14 0 0 0.09 0.08 0.08 0.08
SpindlePole 0 0 0.07 0 0.05 0 0.07 0.07 0 0 0 0 0 0 0 0.12 0.13 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.12 0.05 0 0.06 0 0 0 0 0 0 0 0.07 0.09 0.07 0.16 0.22 0.13 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 4 0 0 0 0 1 0 0 0 0 2 1 1 1 0 1 1 4 5 1 4 7
Bud 2 1 12 12 12 7 25 26 8 24 0 1 2 2 1 7 6 5 0 0 0 0 0 1
Bud Neck 0 0 1 0 1 0 1 1 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 1
Bud Site 3 0 1 2 0 3 7 8 7 9 0 1 0 0 0 1 1 1
Cell Periphery 4 14 5 3 0 2 1 1 0 2 0 1 6 5 6 19 7 10 0 0 0 0 0 0
Cytoplasm 4 1 10 5 11 12 19 23 11 22 5 5 6 7 12 37 30 16 0 0 1 0 0 1
Endoplasmic Reticulum 0 11 9 5 3 4 3 3 5 3 0 0 7 2 10 14 6 8 1 0 0 0 2 2
Endosome 19 1 13 3 9 1 6 4 1 0 0 0 3 4 16 45 37 20 20 14 19 6 7 10
Golgi 0 5 2 0 0 0 0 0 0 0 0 0 16 5 21 11 14 9 10 14 7 5 8 9
Mitochondria 212 215 196 167 221 210 386 410 302 378 26 41 93 147 119 24 20 18 197 193 185 82 113 94
Nucleus 3 0 1 1 1 4 8 12 6 8 0 0 0 0 0 6 4 1 0 0 0 0 1 0
Nuclear Periphery 2 6 2 1 2 4 12 12 7 12 0 0 1 1 1 3 2 0 3 1 2 0 0 1
Nucleolus 8 0 0 1 1 2 6 15 2 6 1 0 0 1 0 2 1 1 2 0 0 0 1 2
Peroxisomes 21 6 18 4 7 2 8 6 3 0 0 0 5 13 11 29 19 16 7 9 23 8 12 11
SpindlePole 3 0 17 3 13 8 28 33 5 13 1 2 0 1 2 26 21 9 1 0 1 2 2 2
Vac/Vac Membrane 23 28 11 4 16 6 18 10 8 6 0 1 8 15 11 35 36 15 4 9 3 3 4 2
Unique Cell Count 242 228 227 180 253 227 422 458 315 402 31 45 116 171 168 218 166 112 255 255 256 112 162 155
Labelled Cell Count 304 289 302 211 297 265 528 565 365 483 33 52 149 204 211 260 205 130 255 255 256 112 162 155


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 5.0 4.9 4.3 4.4 4.0 3.7 4.0 3.6 3.6 4.2 3.5 5.9 5.7 5.7 5.6 6.5 7.0 4.8 5.2 6.5
Std Deviation (1e-4) 1.2 0.8 1.2 1.0 1.1 1.2 1.3 1.3 0.9 1.3 1.7 1.1 1.1 1.6 1.2 1.4 1.9 1.9 1.0 0.9 1.8
Intensity Change (Log2) -0.18 -0.15 -0.29 -0.41 -0.28 -0.43 -0.46 -0.21 -0.5 0.27 0.22 0.22 0.2 0.4 0.51 -0.02 0.1 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0 0 0 0 0 0 -2.2 -2.6 -1.1 -0.8 -0.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 3.0 1.1 0
Cyto
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.9 0 0.9 1.2 -0.2 1.3
Endosome 0 0 0 0 0 0 0 -1.3 -1.7 1.4 4.7 4.9 3.5
Golgi 0 0 0 0 0 0 0 5.1 0 4.9 2.6 3.7 0
Mitochondria 0 0 0 0 0 0 0 -1.5 -0.1 -3.8 -15.9 -14.6 -12.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuc
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 -1.3 -0.1 -0.5 1.8 1.2 1.8
SpindlePole 0 0 0 0 0 0 0 -3.0 -3.3 -2.9 1.6 1.7 0.2
Vac
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 0 0.4 0.1 0.4 -0.5 0.6 0.3 -0.2 1.2 4.3 4.4 3.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.4 0.7 -1.2 -0.3 -1.9 -1.4 -2.5 0.8 1.6 0.7 3.9 5.1 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7201 1.873 1.7628 1.656 1.6578 1.7154 2.0532 2.3848 2.2021 2.0251 1.6629 2.405 2.9098 2.5196 2.5593 3.5282 2.9461 2.3059
Actin 0.0334 0.0166 0.0166 0.0425 0.0162 0.0125 0.0978 0.022 0.0641 0.0451 0.0131 0.0613 0.0344 0.0358 0.0243 0.0839 0.0183 0.0197
Bud 0.0029 0.0041 0.0033 0.0026 0.006 0.0009 0.0021 0.0023 0.0043 0.0049 0.0019 0.0041 0.0006 0.0031 0.0017 0.0083 0.0007 0.0009
Bud Neck 0.0046 0.0016 0.0195 0.0059 0.0018 0.0101 0.0028 0.0026 0.0098 0.0023 0.0018 0.0089 0.0011 0.0026 0.0041 0.0041 0.0008 0.0057
Bud Periphery 0.0045 0.0092 0.0093 0.0132 0.0335 0.0047 0.0038 0.0042 0.0131 0.0169 0.0037 0.0059 0.002 0.0049 0.0059 0.0245 0.0012 0.003
Bud Site 0.0239 0.064 0.0214 0.0281 0.0068 0.0021 0.0171 0.0332 0.0277 0.0137 0.0026 0.0039 0.0024 0.0536 0.0228 0.0067 0.0009 0.0028
Cell Periphery 0.0038 0.0095 0.018 0.023 0.007 0.0027 0.0013 0.0014 0.0117 0.0025 0.0004 0.0028 0.0022 0.0025 0.0063 0.0013 0.0004 0.004
Cytoplasm 0.0451 0.0467 0.0388 0.0488 0.0102 0.0206 0.0475 0.0237 0.027 0.0368 0.007 0.0213 0.0161 0.012 0.0215 0.0075 0.0238 0.033
Cytoplasmic Foci 0.1458 0.0286 0.0468 0.0464 0.0476 0.0575 0.1142 0.1105 0.0786 0.0747 0.0675 0.0768 0.1099 0.0783 0.0614 0.0331 0.0734 0.0463
Eisosomes 0.0009 0.0025 0.0043 0.0051 0.0008 0.0006 0.0023 0.0013 0.0076 0.0007 0.0004 0.0012 0.0012 0.0014 0.0037 0.0026 0.003 0.0027
Endoplasmic Reticulum 0.0112 0.0092 0.0066 0.0038 0.0035 0.0023 0.0098 0.0012 0.0042 0.0024 0.001 0.0021 0.0036 0.0044 0.0021 0.0009 0.0036 0.0034
Endosome 0.1297 0.0505 0.0777 0.0905 0.0678 0.0673 0.1 0.0825 0.0723 0.1021 0.0859 0.0624 0.1367 0.0961 0.0462 0.0921 0.165 0.0618
Golgi 0.1111 0.0436 0.042 0.0477 0.0562 0.0517 0.0563 0.0756 0.0503 0.058 0.0812 0.0523 0.0792 0.0708 0.0325 0.0154 0.0881 0.0576
Lipid Particles 0.1217 0.0314 0.0245 0.0107 0.0449 0.0149 0.053 0.0249 0.0338 0.0426 0.0241 0.0205 0.0452 0.0226 0.0345 0.0016 0.0326 0.0301
Mitochondria 0.1522 0.577 0.5386 0.5255 0.5856 0.6526 0.2142 0.4809 0.4011 0.4371 0.4987 0.5477 0.4063 0.4809 0.6093 0.5723 0.4085 0.5955
None 0.0765 0.0522 0.0567 0.0729 0.005 0.0653 0.1239 0.0467 0.0848 0.0397 0.0608 0.0587 0.0406 0.0721 0.0644 0.1155 0.1268 0.0591
Nuclear Periphery 0.0065 0.0023 0.0013 0.0004 0.0011 0.0006 0.0131 0.0004 0.0035 0.0013 0.0012 0.0007 0.002 0.004 0.0004 0.0005 0.0008 0.0006
Nucleolus 0.0161 0.0008 0.0023 0.0007 0.0012 0.0006 0.0029 0.001 0.0014 0.0004 0.0004 0.001 0.0011 0.0015 0.0013 0.0002 0.0005 0.0004
Nucleus 0.0085 0.0012 0.0017 0.0008 0.0016 0.0006 0.0084 0.0007 0.0015 0.0011 0.0006 0.0014 0.0008 0.0016 0.0009 0.0003 0.0006 0.0004
Peroxisomes 0.0683 0.0295 0.0521 0.0164 0.0843 0.0216 0.1025 0.0772 0.0864 0.1042 0.1387 0.0591 0.0908 0.0365 0.0395 0.008 0.0418 0.0651
Punctate Nuclear 0.0051 0.0006 0.0008 0.0002 0.001 0.0006 0.0155 0.0007 0.0009 0.0012 0.0009 0.0017 0.0053 0.0006 0.001 0.0007 0.0013 0.0013
Vacuole 0.0234 0.014 0.0131 0.0113 0.0122 0.0061 0.0086 0.0055 0.0096 0.0071 0.0041 0.0034 0.0103 0.0109 0.0125 0.0102 0.0046 0.0035
Vacuole Periphery 0.0047 0.005 0.0046 0.0036 0.0057 0.004 0.003 0.0018 0.0063 0.0053 0.004 0.0026 0.0084 0.0038 0.0037 0.0103 0.0031 0.0031

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.2738 6.1228 5.452 8.584 8.8803 8.2145 7.7384 10.8636 10.1419 12.3078
Translational Efficiency 0.4772 0.4627 0.7626 0.5793 0.5408 0.8039 0.6633 0.5804 0.7214 0.5558

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Mitochondrial glutamyl-tRNA synthetase; predicted to be palmitoylated
Localization
Cell Percentages mitochondrion (93%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mse1

Mse1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mse1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available