Standard name | |
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Human Ortholog | |
Description | Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 3 | 0 | 1 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 2 | 3 | 5 | 3 | 3 | 6 | – | 3 | 9 | 5 | 6 | 4 | 7 | 9 | 32 | 22 | 31 | 1 | 0 | 2 | 1 | 3 | 2 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 6 | 4 | 9 | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 2 | 3 |
Golgi | 0 | 2 | 1 | 0 | 0 | 6 | 3 | – | 0 | 3 | 2 | 0 | 2 | 0 | 0 | 4 | 15 | 7 | 0 | 0 | 1 | 0 | 0 | 1 |
Mitochondria | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 4 | 3 | 4 | 7 | 0 | 0 | 0 | 2 | 7 | 1 | 0 | 0 | 0 | 2 | 0 | 0 |
Nucleus | 295 | 200 | 128 | 87 | 48 | 70 | 76 | – | 47 | 55 | 67 | 50 | 60 | 51 | 47 | 98 | 75 | 73 | 303 | 268 | 128 | 54 | 86 | 45 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 1 |
Nucleolus | 16 | 8 | 8 | 4 | 10 | 4 | 7 | – | 7 | 22 | 17 | 23 | 6 | 3 | 14 | 10 | 10 | 14 | 22 | 16 | 22 | 5 | 19 | 23 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 22 | 49 | 12 | 8 | 13 | 48 | 48 | – | 28 | 24 | 20 | 28 | 7 | 11 | 19 | 45 | 55 | 72 | 25 | 27 | 13 | 7 | 23 | 16 |
Unique Cell Count | 333 | 253 | 151 | 101 | 73 | 127 | 141 | 88 | 106 | 110 | 110 | 80 | 72 | 88 | 187 | 167 | 187 | 362 | 320 | 177 | 77 | 143 | 100 | |
Labelled Cell Count | 340 | 262 | 152 | 105 | 75 | 131 | 145 | 90 | 117 | 116 | 117 | 82 | 74 | 90 | 199 | 193 | 210 | 362 | 320 | 177 | 77 | 143 | 100 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 37.4 | 32.1 | 27.5 | 25.8 | 15.2 | 24.9 | 22.4 | – | 19.3 | 19.0 | 18.3 | 16.5 | 29.5 | 30.8 | 27.7 | 25.3 | 31.5 | 27.8 | 26.8 | 31.6 | 26.5 |
Std Deviation (1e-4) | 8.2 | 8.1 | 6.5 | 6.0 | 5.1 | 5.9 | 5.5 | – | 5.4 | 5.6 | 4.9 | 5.1 | 9.5 | 9.6 | 9.7 | 10.3 | 11.7 | 12.5 | 9.9 | 11.1 | 10.5 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.86 | -0.15 | -0.3 | – | -0.51 | -0.54 | -0.59 | -0.74 | 0.1 | 0.16 | 0.01 | -0.12 | 0.19 | 0.01 | -0.04 | 0.2 | -0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.5 | 3.7 | 4.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.3 | -3.3 | -5.4 | -5.7 | – | -5.3 | -5.7 | -4.4 | -6.7 | -1.8 | -2.4 | -5.3 | -6.3 | -7.4 | -8.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 2.2 | -0.9 | -0.1 | – | 0.8 | 3.8 | 2.8 | 3.8 | 0 | 0 | 2.7 | 0 | 0.3 | 0.8 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.2 | 6.0 | 5.5 | – | 4.8 | 3.3 | 2.5 | 3.9 | 0.2 | 1.7 | 3.0 | 3.9 | 5.5 | 6.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 19.6141 | 27.4412 | 23.7735 | 23.149 | 18.413 | 22.6824 | 29.001 | 36.8251 | 31.1057 | 30.8683 | 33.9522 | 31.8946 | 23.844 | 31.3153 | 25.2651 | 28.7513 | 26.1396 | 26.6356 |
Actin | 0.0013 | 0 | 0.0002 | 0.0001 | 0.0046 | 0.0007 | 0.02 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0092 | 0 | 0.0066 | 0 | 0.0003 | 0.0005 |
Bud | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0003 | 0.0002 |
Bud Neck | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0008 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0007 | 0.0002 | 0 | 0.0001 | 0 | 0.0005 | 0.0006 |
Bud Periphery | 0.0009 | 0 | 0.0002 | 0 | 0.0009 | 0.0008 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0005 | 0 | 0.0001 | 0 | 0.0004 | 0.0002 |
Bud Site | 0.001 | 0 | 0.0001 | 0.0001 | 0.0009 | 0.0002 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0.001 | 0 | 0.0004 | 0 | 0.0005 | 0.0001 |
Cell Periphery | 0.0003 | 0.0001 | 0.0005 | 0.0003 | 0.0007 | 0.0004 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 |
Cytoplasm | 0.0303 | 0.0067 | 0.0104 | 0.0183 | 0.0034 | 0.0015 | 0.001 | 0.0016 | 0.0014 | 0.0005 | 0.0439 | 0.0003 | 0.0054 | 0.0002 | 0.0078 | 0.0002 | 0.0174 | 0.0038 |
Cytoplasmic Foci | 0.012 | 0 | 0.0005 | 0.0288 | 0.0016 | 0.0004 | 0.0033 | 0.0033 | 0.008 | 0 | 0.0005 | 0 | 0.0147 | 0 | 0.005 | 0 | 0.002 | 0.0014 |
Eisosomes | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0026 | 0 | 0.0003 | 0.0005 | 0.0062 | 0.0017 | 0.0028 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.005 | 0 | 0.0019 | 0 | 0.001 | 0.0011 |
Endosome | 0.0065 | 0 | 0.006 | 0.0017 | 0.0103 | 0.011 | 0.0122 | 0.0001 | 0.0003 | 0.0001 | 0.0008 | 0.0002 | 0.0249 | 0 | 0.0082 | 0 | 0.0011 | 0.009 |
Golgi | 0.001 | 0 | 0.001 | 0.0003 | 0.0013 | 0.0007 | 0.0031 | 0 | 0.0001 | 0 | 0 | 0 | 0.0042 | 0 | 0.005 | 0 | 0.0001 | 0.0005 |
Lipid Particles | 0.0042 | 0 | 0.0002 | 0.006 | 0.0015 | 0.0009 | 0.0034 | 0 | 0.0017 | 0 | 0 | 0 | 0.0079 | 0 | 0.0055 | 0 | 0.0004 | 0.0003 |
Mitochondria | 0.0048 | 0.0006 | 0.0013 | 0.001 | 0.0069 | 0.004 | 0.0032 | 0.0002 | 0.0094 | 0.0023 | 0.0018 | 0.0006 | 0.0035 | 0.0003 | 0.0071 | 0.0003 | 0.0019 | 0.0014 |
None | 0.0024 | 0 | 0.0001 | 0.003 | 0.0021 | 0.0002 | 0.0018 | 0.0001 | 0.0011 | 0 | 0.0001 | 0 | 0.0044 | 0 | 0.0034 | 0 | 0.0004 | 0.0005 |
Nuclear Periphery | 0.0414 | 0.0098 | 0.0105 | 0.0144 | 0.063 | 0.024 | 0.017 | 0.0026 | 0.0132 | 0.0106 | 0.016 | 0.0143 | 0.0216 | 0.0086 | 0.0119 | 0.0029 | 0.0091 | 0.0116 |
Nucleolus | 0.0423 | 0.0068 | 0.0111 | 0.0076 | 0.0878 | 0.0339 | 0.0223 | 0.0059 | 0.0269 | 0.0127 | 0.0278 | 0.0345 | 0.022 | 0.0071 | 0.0078 | 0.0023 | 0.0502 | 0.0119 |
Nucleus | 0.8229 | 0.9751 | 0.9516 | 0.9058 | 0.7764 | 0.9113 | 0.8944 | 0.9492 | 0.9291 | 0.9716 | 0.8988 | 0.945 | 0.8251 | 0.9834 | 0.9182 | 0.9942 | 0.9027 | 0.9486 |
Peroxisomes | 0.0005 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0013 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0012 | 0 | 0.0017 | 0 | 0 | 0.0002 |
Punctate Nuclear | 0.0141 | 0.0001 | 0.0001 | 0.0071 | 0.0139 | 0.0008 | 0.009 | 0.0364 | 0.004 | 0.0004 | 0.0003 | 0.0005 | 0.0415 | 0 | 0.0064 | 0 | 0.0046 | 0.0055 |
Vacuole | 0.0053 | 0.0002 | 0.0039 | 0.0024 | 0.008 | 0.0028 | 0.0014 | 0 | 0.0011 | 0.0002 | 0.0012 | 0.0013 | 0.0038 | 0 | 0.0008 | 0 | 0.0031 | 0.0013 |
Vacuole Periphery | 0.0049 | 0.0004 | 0.0017 | 0.0019 | 0.0092 | 0.0033 | 0.0017 | 0 | 0.002 | 0.0009 | 0.0082 | 0.0019 | 0.0032 | 0.0002 | 0.0019 | 0.0001 | 0.0035 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 134.1394 | 131.5168 | 147.3435 | 137.423 | 134.6458 | 167.221 | 146.0134 | 132.8655 | 166.9565 | 109.9634 |
Translational Efficiency | 1.6032 | 1.6218 | 1.4314 | 1.9577 | 1.2981 | 1.3361 | 1.5221 | 1.6414 | 1.5302 | 1.5004 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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