Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0.06 0.05 0 0 0 0 0 0.07 0.14 0.12 0.06 0.16 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.16 0.18 0.23 0.15 0.3 0.37 0.35 0.5 0.53 0.47 0.47 0 0 0.08 0.08 0 0 0 0 0 0 0 0
Nucleus 0.84 0.87 0.89 0.87 0.88 0.77 0.77 0.79 0.68 0.65 0.7 0.69 0.84 0.81 0.79 0.78 0.71 0.81 0.72 0.88 0.81 0.71 0.7 0.64
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.25 0.21 0.14 0.16 0.1 0.17 0.18 0.19 0.26 0.25 0.29 0.38 0.22 0.19 0.25 0.2 0.13 0.19 0.2 0.06 0.12 0.14 0.2 0.25
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 1 0 0 0 0 0 7 1 3 1 2 0 0 1 0 0 0 1 1 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 2 1 4 6 10 7 4 13 9 11 0 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 4 0 3 12 13 18 23 17 12 10 8 4 12 25 25 5 14 7 0 0 0 0 1 0
Endoplasmic Reticulum 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Endosome 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 2 1 0 3 1 4 1 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 2 2 1 2
Mitochondria 1 18 51 55 48 88 161 170 198 259 149 228 4 6 16 7 3 3 1 0 3 0 0 2
Nucleus 131 96 248 205 278 227 330 387 269 318 225 334 138 145 159 66 61 59 131 101 241 54 152 134
Nuclear Periphery 0 1 2 0 6 3 12 10 8 12 6 11 1 3 2 1 0 0 1 0 0 0 0 0
Nucleolus 39 23 40 37 30 50 78 95 103 122 94 185 36 34 50 17 11 14 37 6 34 10 43 53
Peroxisomes 1 0 0 0 0 0 1 0 0 0 0 0 3 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 1 1 4 1 0 2 1 1 2 1 0 0 1 0 2 0 1 0 1 1
Vac/Vac Membrane 1 1 2 1 3 1 0 1 1 2 1 1 1 2 1 3 1 3 1 3 6 3 11 9
Unique Cell Count 156 110 278 236 315 296 430 490 396 493 320 482 164 180 201 85 86 73 183 115 298 77 217 211
Labelled Cell Count 179 141 348 311 384 394 621 695 596 742 495 777 198 217 257 102 95 86 183 115 298 77 217 211


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.1 5.7 5.4 5.1 5.6 4.9 4.7 4.7 4.3 4.3 4.2 4.2 8.0 7.1 6.5 9.5 10.2 10.7 7.4 8.2 8.6
Std Deviation (1e-4) 1.3 1.0 1.0 1.2 1.3 1.4 1.4 1.3 1.2 1.1 1.1 1.3 1.9 2.1 1.8 1.9 2.6 2.3 1.4 1.6 1.6
Intensity Change (Log2) – – – -0.09 0.04 -0.14 -0.2 -0.22 -0.35 -0.32 -0.36 -0.37 0.55 0.38 0.27 0.81 0.91 0.98 0.45 0.6 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.7 2.3 3.2 2.9 2.0 1.7 0 1.3 0 3.5 5.6 5.2 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.4 -1.0 3.2 0 0 0 0 0 0 -4.9 -4.8 -3.2 -2.2 -3.4 -3.0
Nucleus -0.8 -0.4 -4.0 -4.2 -3.6 -6.4 -7.5 -5.7 -6.2 -1.5 -2.6 -3.1 -2.7 -4.1 -1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.4 -1.8 0.8 0 0 0 0 0 0 2.0 1.3 2.9 1.2 -0.4 1.0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0836 4.5921 3.6597 3.5647 3.5617 3.5954 3.5369 4.6639 4.3048 4.7434 3.951 4.3409 6.6971 5.8579 5.4624 5.2825 5.8723 5.32
Actin 0.0514 0.0031 0.0119 0.038 0.019 0.0001 0.0354 0.0027 0.0449 0.0155 0.0032 0.036 0.018 0 0.0004 0.0018 0.0001 0
Bud 0.0007 0.0004 0.0016 0.0002 0.0006 0.0001 0.0003 0.0001 0.0007 0.0002 0.0029 0.0005 0.0003 0 0 0.0002 0 0
Bud Neck 0.0104 0.0002 0.001 0.0004 0.0027 0.0011 0.0007 0.0002 0.0004 0.0001 0.002 0.0015 0.0003 0.0001 0.0001 0.0006 0.0011 0.001
Bud Periphery 0.0018 0.0001 0.0026 0.0004 0.0019 0.0003 0.0004 0.0001 0.0012 0.0002 0.0052 0.001 0.0004 0 0 0.0003 0.0001 0.0001
Bud Site 0.01 0.0007 0.005 0.0006 0.0036 0.0001 0.0014 0.0004 0.002 0.0003 0.0067 0.0006 0.0003 0 0.0001 0.0015 0.0002 0.0001
Cell Periphery 0.001 0.0001 0.0005 0.0002 0.0007 0.0002 0.0001 0 0.0002 0 0.0004 0.0001 0.0001 0 0 0.0001 0 0
Cytoplasm 0.0018 0.001 0.0005 0.0001 0.0003 0.0002 0.028 0 0.0012 0.0051 0.0161 0.0013 0.0084 0 0.0015 0.0005 0.0001 0
Cytoplasmic Foci 0.0148 0.0006 0.0097 0.0022 0.0101 0.0001 0.0159 0.0028 0.0039 0.0047 0.003 0.003 0.0007 0 0.0048 0.0071 0 0
Eisosomes 0.0009 0 0.0002 0.0003 0.0007 0 0.0002 0 0.0002 0.0001 0.0001 0.0001 0.0005 0 0 0.0001 0 0
Endoplasmic Reticulum 0.004 0.0008 0.0013 0.0005 0.0007 0.0001 0.0026 0 0.0023 0.0022 0.0048 0.0018 0.006 0 0.002 0.0006 0 0
Endosome 0.0231 0.0012 0.0136 0.0054 0.0124 0.0002 0.0106 0.0002 0.0035 0.0131 0.0153 0.0082 0.0053 0 0.0084 0.0024 0.0001 0
Golgi 0.0144 0.0002 0.0076 0.0088 0.0045 0 0.0046 0.0007 0.0026 0.0016 0.0014 0.0051 0.0006 0 0.0006 0.0011 0 0
Lipid Particles 0.0232 0.0001 0.0161 0.0361 0.0063 0.0006 0.0179 0.0004 0.0039 0.0021 0.0011 0.0047 0.0006 0 0.0054 0.0067 0.0001 0
Mitochondria 0.0217 0.0005 0.0296 0.0714 0.0439 0.0062 0.0025 0.0002 0.0132 0.0053 0.0124 0.0053 0.0023 0.0001 0.0003 0.002 0.0004 0.0001
None 0.0017 0.0005 0.0006 0.0002 0.0004 0.0001 0.0223 0 0.0005 0.0036 0.0041 0.0007 0.0623 0 0.0005 0.0008 0.0001 0
Nuclear Periphery 0.0147 0.0029 0.0058 0.0011 0.0032 0.0031 0.0207 0.0066 0.009 0.042 0.0222 0.0127 0.0338 0.0005 0.0164 0.015 0.0033 0.0002
Nucleolus 0.0614 0.0522 0.0541 0.0213 0.3127 0.1593 0.0425 0.0515 0.0328 0.0134 0.1205 0.1131 0.0324 0.0326 0.0303 0.0365 0.1754 0.1137
Nucleus 0.7016 0.934 0.8153 0.8054 0.5492 0.8259 0.7314 0.9259 0.854 0.8695 0.7486 0.79 0.8212 0.9664 0.907 0.8925 0.81 0.8835
Peroxisomes 0.0151 0 0.0073 0.0037 0.0109 0.0001 0.0144 0.0066 0.004 0.0034 0.0008 0.0038 0.0002 0 0.0006 0.0011 0 0
Punctate Nuclear 0.0129 0.0008 0.0027 0.0006 0.0024 0.0009 0.0468 0.001 0.0173 0.0161 0.0186 0.0092 0.0038 0.0002 0.0209 0.0279 0.0085 0.0013
Vacuole 0.0076 0.0004 0.0061 0.0011 0.0064 0.0006 0.0008 0.0002 0.0014 0.0004 0.0083 0.0007 0.0008 0 0.0003 0.0006 0.0002 0.0001
Vacuole Periphery 0.0054 0.0001 0.0067 0.0019 0.0072 0.0008 0.0005 0.0003 0.0008 0.0012 0.0022 0.0006 0.0017 0 0.0003 0.0006 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.4748 26.1332 27.5095 38.0522 28.8679 21.7433 36.1783 42.2 33.6645 37.6987
Translational Efficiency 1.3673 1.3928 1.3268 0.7421 1.0421 1.5925 0.9326 0.8873 1.216 1.0439

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Gal11

Gal11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gal11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available