Standard name
Human Ortholog
Description Arginosuccinate synthetase; catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0.06 0.07 0.05 0.05 0.06 0 0.05 0 0.26 0.23 0.32 0 0 0 0 0 0
Cytoplasm 1.0 – 1.0 1.0 0.99 0.99 0.94 0.89 0.88 0.89 0.88 0.88 0.99 0.99 0.99 0.86 0.79 0.78 0.92 0.9 0.95 0.96 0.97 0.96
Endoplasmic Reticulum 0 – 0 0 0 0 0.05 0.07 0.08 0.08 0.07 0.08 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 2 2 2 5 5 1 2 0 0 1 0 0 0 2 0 0 2 0 6
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 1 2 4 12 24 23 19 12 16 12 21 18 24 17 37 0 0 0 0 0 0
Cytoplasm 200 – 240 210 278 363 441 346 305 373 198 240 343 395 421 81 59 89 180 27 223 265 363 452
Endoplasmic Reticulum 0 – 0 0 1 0 23 28 27 34 16 22 1 0 1 2 3 8 2 0 2 0 0 2
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 1 0 0 0 0 0 0 0 0 0 0 2 0 0 2 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 – 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 2 0 0 0 2 0 0 1 0 3 2 3
Unique Cell Count 200 240 211 281 366 468 387 348 421 224 273 347 399 425 94 75 114 195 30 236 278 376 472
Labelled Cell Count 200 240 213 282 369 478 400 362 432 227 280 358 416 443 107 81 136 195 30 236 278 376 472


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 360.0 – 376.3 339.7 286.7 249.0 209.9 197.0 181.6 168.5 159.0 156.9 389.3 385.5 385.1 530.0 529.1 559.3 363.9 358.2 369.4
Std Deviation (1e-4) 92.9 – 75.3 65.1 68.6 54.6 57.4 58.3 55.3 51.9 57.9 57.4 92.3 92.3 105.8 177.9 173.4 179.1 85.4 96.6 99.3
Intensity Change (Log2) – – – -0.15 -0.39 -0.6 -0.84 -0.93 -1.05 -1.16 -1.24 -1.26 0.05 0.03 0.03 0.49 0.49 0.57 -0.05 -0.07 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 3.9 4.1 3.3 3.6 3.8 0 3.6 3.2 8.1 0 9.3
Cytoplasm -1.1 -1.6 -1.4 -3.8 -5.2 -5.7 -5.4 -5.4 -5.6 -1.7 -1.6 -1.5 -5.9 -7.3 -7.5
Endoplasmic Reticulum 0 0 0 3.5 4.3 4.4 4.5 4.2 4.5 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0009 0.0004 0.0011 0.0013 0.0002 0.0004 0.0107 0.0002 0.0025 0.0006 0.0028 0.0003 – – – – – –
Bud 0.0009 0.0004 0.0006 0.0003 0.0002 0.0002 0.0023 0.003 0.0018 0.0003 0.0022 0.0007 – – – – – –
Bud Neck 0.0003 0.0002 0.002 0.0005 0.0003 0.0004 0.0004 0.0001 0.0011 0.0014 0.0003 0.0007 – – – – – –
Bud Periphery 0.0003 0.0001 0.0026 0.0001 0 0 0.001 0.0018 0.0017 0.0003 0.0011 0.0006 – – – – – –
Bud Site 0.0006 0.0001 0.0008 0.0004 0 0 0.0018 0.0005 0.0038 0.0013 0.0011 0.0002 – – – – – –
Cell Periphery 0.0015 0.0002 0.0162 0.0004 0.0001 0 0.0024 0.0004 0.0023 0.0005 0.0025 0.0005 – – – – – –
Cytoplasm 0.8057 0.9691 0.9173 0.8772 0.9566 0.95 0.7142 0.9826 0.8674 0.808 0.6245 0.9094 – – – – – –
Cytoplasmic Foci 0.0334 0.0002 0.0069 0.0305 0.0001 0.0003 0.0306 0.0001 0.0194 0.029 0.043 0.0005 – – – – – –
Eisosomes 0.0012 0 0.0013 0.0008 0 0 0.0022 0 0.0008 0.0007 0.0047 0 – – – – – –
Endoplasmic Reticulum 0.0006 0.0001 0.0007 0.0002 0.0001 0 0.0047 0.0001 0.0025 0.0007 0.0017 0.0002 – – – – – –
Endosome 0.0005 0 0.0001 0.0003 0 0 0.0169 0 0.0076 0.0097 0.0671 0.0005 – – – – – –
Golgi 0.0002 0 0.0001 0.0005 0 0 0.0042 0 0.0023 0.001 0.0143 0 – – – – – –
Lipid Particles 0.0049 0 0.0028 0.0108 0 0 0.0078 0 0.0068 0.0041 0.0188 0.0001 – – – – – –
Mitochondria 0.0178 0.0022 0.007 0.0061 0.0007 0.0007 0.0266 0.0011 0.0128 0.0073 0.093 0.003 – – – – – –
None 0.012 0.0014 0.0027 0.0057 0.0004 0.0006 0.0299 0.0005 0.0254 0.0242 0.0561 0.0014 – – – – – –
Nuclear Periphery 0.015 0.0022 0.0034 0.011 0.0052 0.0049 0.0423 0.001 0.0046 0.0057 0.0112 0.0042 – – – – – –
Nucleolus 0.0463 0.0012 0.0107 0.0086 0.0001 0.0001 0.0372 0.0001 0.01 0.0653 0.0203 0.0184 – – – – – –
Nucleus 0.0479 0.0208 0.0181 0.0372 0.0342 0.042 0.0417 0.008 0.0153 0.0287 0.0153 0.0499 – – – – – –
Peroxisomes 0.001 0.0001 0.0005 0.0015 0 0 0.0029 0 0.0015 0.0006 0.0056 0.0001 – – – – – –
Punctate Nuclear 0.0019 0.0001 0.0002 0.0039 0.0001 0.0004 0.0091 0 0.0012 0.0041 0.002 0.0005 – – – – – –
Vacuole 0.0039 0.0008 0.0037 0.001 0.0008 0.0001 0.0057 0.0004 0.0066 0.0044 0.0059 0.0048 – – – – – –
Vacuole Periphery 0.0034 0.0005 0.001 0.0018 0.0007 0.0001 0.0053 0.0001 0.0027 0.002 0.0066 0.004 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 447.7393 338.1638 227.1718 249.3192 269.0185 733.4494 754.9024 658.4701 671.1573 529.7273
Translational Efficiency 1.3823 1.4341 1.4563 1.5238 1.6817 1.4117 1.3609 1.3884 1.137 1.2294

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Arginosuccinate synthetase; catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate
Localization
Cell Percentages cytoplasm (74%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Arg1

Arg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available