Standard name
Human Ortholog
Description NAD-dependent glycerol 3-phosphate dehydrogenase; expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1; GPD2 has a paralog, GPD1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0.11 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.99 1.0 0.99 0.94 0.91 0.81 0.87 0.82 0.82 0.84 1.0 0.99 1.0 0.99 0.99 0.98 0.96 0.97 0.95 0.96 0.96 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.08 0.07 0.11 0.07 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 3 3 4 12 6 0 1 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 4 0 1 4 0 4 0 0 1 0 0 0 2 0 1 1 4 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 3 1 4 12 22 16 19 46 20 15 0 2 2 1 3 2 0 0 0 0 0 0
Cytoplasm 127 96 311 204 241 302 467 289 324 358 216 276 170 218 265 175 92 96 114 93 290 100 229 303
Endoplasmic Reticulum 0 1 2 2 0 10 26 30 26 49 18 32 0 2 1 3 4 1 0 0 4 0 0 2
Endosome 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1 0 0 0 0 0 0 4 1 1 1 3 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 2 0 0 0 0 0 2 4 7 5 14 9 0 0 0 1 0 0 0 0 1 0 1 0
Nucleus 0 0 0 0 0 1 0 1 3 2 1 2 0 0 0 0 0 2 0 1 0 0 1 4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 1 0 1 1 4 24 6 4 4 0 0 0 1 3 0 2 0 0 2 1 0 3
Unique Cell Count 130 97 315 205 243 322 512 359 374 435 263 330 170 220 265 177 93 98 120 96 307 105 239 319
Labelled Cell Count 131 98 317 207 247 328 525 372 390 474 288 347 170 223 270 184 99 103 120 96 307 105 239 319


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 38.6 62.7 55.3 53.9 47.6 38.1 34.1 30.8 30.0 28.4 26.0 25.8 68.4 69.8 64.1 82.0 85.9 75.5 57.8 60.8 60.1
Std Deviation (1e-4) 9.9 13.2 12.2 11.9 11.4 9.1 7.7 7.4 7.0 6.0 5.8 5.7 17.4 18.3 13.7 28.5 28.1 33.8 14.0 18.6 17.2
Intensity Change (Log2) – – – -0.04 -0.22 -0.54 -0.7 -0.85 -0.88 -0.96 -1.09 -1.1 0.31 0.33 0.21 0.57 0.63 0.45 0.06 0.14 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 3.8 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2.3 2.7 2.7 3.1 5.3 4.1 2.8 0 0 0 0 0 0
Cytoplasm 0.9 0.5 -3.3 -4.4 -7.6 -5.9 -7.2 -7.0 -6.7 1.5 0.4 1.8 0.1 0.1 -0.6
Endoplasmic Reticulum 0 0 2.3 3.4 4.7 4.2 5.7 4.1 5.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 4.1 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 4.4 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 58.4901 73.8611 66.0082 67.554 55.9921 65.8246 58.1601 80.0481 67.3403 64.5176 65.4749 74.3506 65.0105 84.3561 81.5726 73.436 61.1841 79.4984
Actin 0.0088 0.0001 0.0061 0 0.0026 0.0001 0.0095 0 0.0112 0.0099 0 0.0008 0.0066 0.0001 0.0009 0.0002 0.0119 0.0004
Bud 0.0002 0 0.0001 0 0.0008 0.0001 0.0006 0 0.0003 0.0006 0 0 0.0014 0.0001 0.0003 0.0004 0.001 0.0001
Bud Neck 0.0002 0.0001 0.0026 0.0001 0.0006 0.0002 0.0009 0 0.0002 0.0002 0.0001 0.0002 0.0049 0.0001 0.0001 0.0002 0.0013 0.0006
Bud Periphery 0.0001 0 0.0001 0 0.0005 0 0.0005 0 0.0003 0.0006 0 0 0.0009 0 0.0003 0.0001 0.0019 0.0001
Bud Site 0.0002 0 0.0003 0 0.0008 0 0.0025 0 0.0011 0.0016 0 0.0001 0.0029 0 0.0002 0.0001 0.0034 0.0001
Cell Periphery 0.0002 0 0 0 0.0004 0 0.0005 0 0.0003 0.0002 0 0 0.0011 0 0.0003 0.0001 0.0004 0.0001
Cytoplasm 0.9359 0.9969 0.9734 0.9933 0.8106 0.987 0.9032 0.9982 0.9203 0.9343 0.9872 0.9365 0.8816 0.9901 0.9499 0.9508 0.7999 0.9623
Cytoplasmic Foci 0.0068 0 0.0008 0.0001 0.04 0.0003 0.012 0 0.0082 0.0062 0.0001 0.0035 0.0124 0.0014 0.011 0.0004 0.0164 0.0014
Eisosomes 0.0001 0 0.0001 0 0.0004 0 0.0001 0 0.0002 0.0001 0 0 0.0003 0 0.0008 0.0001 0.0003 0
Endoplasmic Reticulum 0.001 0 0.001 0 0.0007 0.0002 0.0032 0 0.0007 0.0013 0 0.0001 0.0022 0 0.0022 0.0005 0.0039 0.0003
Endosome 0.0024 0 0.0025 0 0.0029 0.0008 0.0057 0 0.0058 0.0072 0 0.0009 0.0089 0 0.004 0.0015 0.0325 0.0002
Golgi 0.001 0 0.0007 0 0.0013 0 0.0013 0 0.0049 0.0115 0 0.0021 0.0022 0 0.0016 0.0001 0.0038 0
Lipid Particles 0.0041 0 0.0007 0 0.0186 0 0.0049 0 0.0039 0.0014 0 0.0177 0.0094 0.0006 0.0028 0.0004 0.0067 0.0001
Mitochondria 0.0015 0 0.0002 0 0.0391 0.0001 0.0067 0 0.0247 0.0074 0 0.0059 0.0115 0.0042 0.0028 0.0008 0.0173 0.0006
None 0.0043 0 0.0013 0.0001 0.0063 0.0001 0.0065 0 0.0026 0.0087 0.0001 0.0001 0.0067 0.0002 0.0084 0.0224 0.0112 0.0029
Nuclear Periphery 0.0047 0.0003 0.0038 0.0007 0.0066 0.0015 0.0045 0.0001 0.0011 0.002 0.001 0.0004 0.0047 0.0002 0.0021 0.0038 0.0294 0.0016
Nucleolus 0.0015 0 0.0001 0 0.0173 0 0.0071 0 0.0039 0.0003 0 0 0.0034 0.0004 0.002 0.0008 0.0177 0.009
Nucleus 0.0181 0.0025 0.0045 0.0053 0.0227 0.0069 0.0134 0.0014 0.0037 0.0033 0.0115 0.0037 0.016 0.0018 0.0043 0.0099 0.0195 0.0177
Peroxisomes 0.0021 0 0.0006 0 0.0051 0 0.0015 0 0.0011 0.0004 0 0.0274 0.0075 0.0004 0.0011 0.0001 0.0016 0
Punctate Nuclear 0.0036 0 0.0008 0 0.0064 0.0001 0.0064 0 0.0008 0.001 0 0.0003 0.009 0 0.0012 0.0011 0.011 0.0013
Vacuole 0.0018 0 0.0003 0.0001 0.0068 0.0018 0.0054 0 0.0027 0.0011 0 0.0001 0.0042 0.0001 0.0021 0.0039 0.0043 0.0007
Vacuole Periphery 0.0013 0 0.0002 0.0001 0.0096 0.0006 0.0035 0 0.0021 0.0007 0 0.0001 0.0022 0.0002 0.0019 0.0025 0.0045 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 212.2573 228.887 131.6706 146.4863 117.3253 222.5593 257.6232 225.6474 188.1657 150.5803
Translational Efficiency 1.2583 1.423 1.4124 1.3177 1.3905 1.5218 1.4969 1.4532 1.4608 1.3343

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description NAD-dependent glycerol 3-phosphate dehydrogenase; expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1; GPD2 has a paralog, GPD1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Gpd2

Gpd2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gpd2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available