Standard name
Human Ortholog
Description Protein required for flavinylation of Sdh1p; binds to Sdh1p and promotes FAD cofactor attachment, which is necessary for succinate dehydrogenase (SDH) complex assembly and activity; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.06 0.07 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0.08 0.12 0 0 0 0 0 0
Cytoplasm 0.1 0.08 0.06 0.05 0 0.05 – 0 0 0 0 0 0.12 0.09 0.08 0.1 0.14 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0.11 0 0 0.07 0 – 0 0 0 0 0 0.07 0.13 0.09 0.17 0.11 0.11 0.06 0.08 0.12 0.05 0.08 0.08
Golgi 0 0.05 0 0 0 0 – 0 0 0 0 0 0.14 0.13 0.09 0.15 0.09 0.07 0 0 0 0.05 0.06 0.06
Mitochondria 0.88 0.71 0.94 0.95 0.94 0.9 – 0.92 0.96 0.92 0.96 0.95 0.7 0.72 0.68 0.1 0.08 0.1 0.72 0.7 0.67 0.68 0.6 0.56
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.1 – 0.09 0.06 0.06 0.07 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.05 0.05 – 0.09 0.07 0.07 0.06 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.05 0.28 0.18 0.27 0 0 0.08 0.07 0.08 0.05
SpindlePole 0 0 0.07 0 0.05 0 – 0 0 0 0 0 0 0 0 0.08 0.15 0.09 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.23 0 0 0.06 0 – 0 0 0 0 0 0.09 0.07 0.14 0.21 0.2 0.22 0.06 0.05 0 0 0.05 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 1 0 0 2 1 0 0 2 0 3 9 1 2 6 5
Bud 7 4 8 9 5 2 – 3 6 5 8 6 2 2 0 2 9 5 4 2 0 0 0 3
Bud Neck 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 3 2 1 0 1 2
Bud Site 2 1 2 0 2 0 – 4 3 3 4 5 0 0 0 1 2 2 – – – – – –
Cell Periphery 3 11 0 0 1 2 – 0 0 2 1 2 5 3 1 12 11 20 0 1 0 0 0 0
Cytoplasm 27 21 8 6 2 6 – 8 5 7 3 4 24 12 12 18 19 22 5 2 1 4 3 3
Endoplasmic Reticulum 3 4 0 0 0 1 – 0 0 1 2 1 7 2 5 5 4 10 3 2 0 2 1 1
Endosome 5 30 1 0 11 0 – 0 0 1 1 0 14 17 14 30 15 18 18 27 18 8 15 15
Golgi 0 13 0 1 2 0 – 0 0 0 0 0 28 17 13 26 13 11 4 14 2 8 10 10
Mitochondria 237 198 130 120 145 115 – 176 198 185 221 249 138 97 102 17 11 16 208 246 99 116 106 111
Nucleus 5 2 3 0 1 0 – 2 3 1 2 1 0 1 1 6 7 8 0 0 0 1 1 2
Nuclear Periphery 1 4 4 2 4 13 – 17 13 12 16 23 1 2 2 2 1 1 2 1 0 3 0 2
Nucleolus 2 2 1 2 7 6 – 18 14 14 14 27 1 0 0 3 5 5 1 0 0 1 0 3
Peroxisomes 7 9 1 1 5 0 – 1 0 1 2 2 7 7 8 49 24 44 8 14 11 11 14 10
SpindlePole 6 2 10 0 8 1 – 5 1 2 5 4 1 2 2 15 20 15 3 2 2 2 4 6
Vac/Vac Membrane 49 63 5 2 10 3 – 4 5 6 3 4 18 9 21 37 28 37 18 18 2 3 8 15
Unique Cell Count 270 279 138 126 155 128 191 206 201 231 262 197 135 150 177 137 166 290 351 149 170 179 198
Labelled Cell Count 354 364 173 143 203 149 238 248 242 282 328 248 172 181 223 171 214 290 351 149 170 179 198


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.8 3.8 4.3 5.7 5.6 – 5.4 5.2 5.3 5.2 5.1 5.7 6.1 6.2 7.6 7.9 7.3 5.8 6.2 6.2
Std Deviation (1e-4) 1.0 1.1 0.8 1.1 1.7 1.9 – 2.3 1.8 1.8 1.7 1.7 1.1 1.3 1.3 1.9 2.6 1.9 1.6 2.2 1.7
Intensity Change (Log2) – – – 0.21 0.61 0.58 – 0.53 0.46 0.5 0.47 0.45 0.61 0.71 0.72 1.02 1.06 0.97 0.63 0.73 0.73


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -1.1 0 – 0 -1.3 -1.6 -1.1 0 0 0 0 0 0.3 -1.2
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 3.1 3.4 4.2
Cytoplasm -0.4 0 -0.4 – -0.7 -1.6 -1.0 0 0 2.0 1.0 0.7 1.4 2.2 2.2
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.7 0 – 0 0 0 0 0 2.8 4.0 3.3 4.8 3.6 3.6
Golgi 0 0 0 – 0 0 0 0 0 4.6 4.3 3.5 4.7 3.7 0
Mitochondria 0 -0.2 -1.3 – -0.7 0.8 -0.8 0.6 0.4 -5.4 -4.9 -5.6 -14.9 -14.3 -14.7
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.4 – 2.2 1.4 1.3 1.7 2.2 0 0 0 0 0 0
Nucleolus 0 0 0 – 3.3 2.7 2.7 2.5 3.6 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 6.5 4.8 6.3
SpindlePole 0 -0.7 -2.6 – -2.0 0 0 -2.4 0 0 -2.3 -2.5 0.4 2.0 0.6
Vacuole 0 1.1 0 – 0 0 0 0 0 2.0 1.1 3.1 4.5 4.3 4.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9287 3.0509 2.9169 2.4119 3.1558 2.9544 1.6829 1.7584 1.5431 1.7037 1.5848 1.698 4.5124 4.8271 4.3566 4.1468 4.3036 4.4292
Actin 0.0027 0.0018 0.0011 0.0002 0.0004 0.0008 0.0313 0.012 0.0175 0.0087 0.0073 0.0085 0.0023 0.0098 0.004 0.0014 0.0018 0.0021
Bud 0.0001 0.0002 0.0003 0 0.0001 0.0001 0.0009 0.0019 0.0015 0.0045 0.0017 0.0007 0.0003 0.0009 0.0005 0.0002 0.0001 0.0004
Bud Neck 0.0001 0.0001 0.0001 0 0 0.0001 0.0006 0.0006 0.0006 0.002 0.0003 0.0007 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001
Bud Periphery 0.0003 0.0005 0.0009 0.0001 0.0002 0.0002 0.0013 0.0018 0.0031 0.0056 0.0047 0.0016 0.0009 0.0024 0.0026 0.0012 0.0003 0.0022
Bud Site 0.0006 0.0012 0.0011 0 0.0001 0.0001 0.0024 0.0171 0.0085 0.0268 0.0007 0.001 0.0007 0.0017 0.0004 0.0003 0.0002 0.0001
Cell Periphery 0.0001 0.0001 0.0003 0 0.0001 0.0001 0.0003 0.001 0.0004 0.0004 0.0003 0.0004 0.0002 0.0002 0.0003 0.0001 0.0001 0.0005
Cytoplasm 0.0009 0.0002 0.0004 0 0.0002 0.0002 0.0042 0.0016 0.0025 0.0014 0.0014 0.0032 0.0045 0.0006 0.0005 0.0002 0.0004 0.0006
Cytoplasmic Foci 0.0078 0.0125 0.0075 0.0021 0.0033 0.0066 0.0219 0.0288 0.0192 0.0242 0.0107 0.0266 0.012 0.0106 0.0073 0.0117 0.0177 0.0091
Eisosomes 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0005 0.0003 0.0016 0.0003 0.0002 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0004 0.0004 0.0001 0 0.0001 0.0003 0.0029 0.0007 0.0007 0.0004 0.0007 0.0025 0.0008 0.0002 0.0003 0.0001 0.0001 0.0003
Endosome 0.0273 0.0248 0.0179 0.0088 0.022 0.0126 0.054 0.0498 0.031 0.0226 0.0481 0.0183 0.0264 0.0322 0.0194 0.0254 0.0186 0.0193
Golgi 0.0276 0.0661 0.0158 0.0123 0.0263 0.0218 0.0472 0.0702 0.031 0.0427 0.0643 0.0338 0.0353 0.0462 0.0288 0.0279 0.0471 0.0293
Lipid Particles 0.0114 0.0071 0.0046 0.0011 0.0015 0.01 0.0351 0.0155 0.0045 0.0176 0.0305 0.0075 0.0297 0.0063 0.0025 0.0148 0.0034 0.0101
Mitochondria 0.8856 0.8582 0.926 0.9658 0.9325 0.9215 0.7207 0.7544 0.8233 0.7985 0.8085 0.8531 0.845 0.8554 0.9038 0.8601 0.8431 0.9008
None 0.001 0.0001 0.0001 0 0.0001 0.0002 0.0051 0.0003 0.0092 0.0008 0.001 0.0017 0.0018 0.0012 0.0004 0.0002 0.0001 0.0013
Nuclear Periphery 0.001 0.0016 0.0001 0 0 0.0007 0.0169 0.0003 0.0035 0.0003 0.0004 0.0029 0.0008 0.0001 0.0001 0.0001 0 0.0009
Nucleolus 0.0003 0.0001 0.0002 0 0.0001 0.0001 0.0004 0.0006 0.0003 0.0012 0.0001 0.0003 0.0003 0.0003 0.0001 0.0003 0 0.0001
Nucleus 0.0006 0.0001 0.0001 0 0 0.0001 0.006 0.0002 0.0005 0.0007 0.0002 0.0002 0.0004 0.0003 0.0001 0.0001 0 0.0001
Peroxisomes 0.0261 0.0207 0.0167 0.0076 0.0101 0.0216 0.0366 0.0339 0.0332 0.0336 0.0132 0.0317 0.0303 0.024 0.0247 0.0508 0.0658 0.0198
Punctate Nuclear 0.0004 0 0 0 0 0.0001 0.0017 0.0003 0.0033 0.0025 0.0003 0.0013 0.0002 0.0021 0 0.0001 0 0.0001
Vacuole 0.0028 0.0013 0.004 0.0005 0.0012 0.0009 0.0057 0.0048 0.0028 0.0025 0.0022 0.0014 0.0028 0.0028 0.0019 0.0024 0.0003 0.0009
Vacuole Periphery 0.0027 0.0027 0.0026 0.0011 0.0018 0.0019 0.0042 0.0039 0.0019 0.0026 0.0031 0.0024 0.0052 0.0024 0.0019 0.0022 0.0006 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.154 4.0343 10.4776 13.2783 5.8512 27.0624 17.8458 21.3363 26.586 20.8738
Translational Efficiency 3.0612 4.9308 2.4482 2.9355 4.9703 1.946 2.2933 2.4158 2.0914 2.3417

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein required for flavinylation of Sdh1p; binds to Sdh1p and promotes FAD cofactor attachment, which is necessary for succinate dehydrogenase (SDH) complex assembly and activity; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma)
Localization
Cell Percentages mitochondrion (87%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Sdh5

Sdh5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sdh5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available