Standard name
Human Ortholog
Description Putative RNA exonuclease; possibly involved in pre-rRNA processing and ribosome assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.06 0 0 0.06 0.06 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0.06 0 0 0 0.06 0.08 0.11 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0.08 0.05 0.1 0.11 0.08 0.07 0 0.1 0.11 0.16 0.19 0.06 0.06 0.07 0 0 0 0 0 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.05 0.05 0.06 0 0.1 0.09 0.06 0 0 0 0 0.08 0.07 0.15 0 0 0 0 0.07 0.07 0.07 0.16 0.16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.24 0 0.06 0.31 0.21 0.19 0.2 0.54 0.35 0.56 0.55 0 0 0 0.09 0.07 0 0 0 0 0 0 0.05
Nucleus 0.42 0.31 0.23 0.21 0.33 0.16 0.15 0.11 0.12 0.15 0.16 0.14 0.1 0.15 0.16 0.18 0.21 0.24 0.21 0.17 0.1 0.15 0.1 0.09
Nuclear Periphery 0.05 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0
Nucleolus 0.53 0.56 0.64 0.69 0.39 0.3 0.24 0.23 0.25 0.25 0.25 0.24 0.75 0.63 0.59 0.75 0.73 0.68 0.56 0.48 0.4 0.42 0.32 0.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.16 0.43 0.48 0.3 0.55 0.53 0.5 0.35 0.5 0.33 0.33 0.54 0.46 0.41 0.16 0.19 0.19 0.05 0.14 0.29 0.17 0.19 0.2
Vac/Vac Membrane 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 2 0 1 12 9 10 21 26 15 11 23 0 2 1 1 0 1 1 7 0 1 6 4
Bud Neck 0 2 3 2 4 3 14 18 6 11 9 14 9 6 0 1 0 1 0 3 2 1 4 5
Bud Site 2 8 0 4 12 9 11 14 23 28 33 22 0 1 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 4 6 13 10 17 18 45 42 35 23 13 34 22 37 61 21 14 21 0 1 0 4 6 15
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 8 0 0 2 0 2 1
Endosome 1 25 12 14 2 33 38 24 10 5 11 8 17 17 48 16 10 13 7 42 18 11 49 49
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 1 0 0 0 3 3
Mitochondria 15 131 5 13 67 69 84 75 224 118 167 192 2 1 2 33 17 12 1 16 4 4 9 14
Nucleus 82 168 58 49 71 55 67 43 52 52 49 50 20 35 52 64 51 70 49 99 29 24 29 27
Nuclear Periphery 10 49 5 0 0 3 0 0 1 1 1 1 0 0 0 6 3 3 22 22 8 3 5 6
Nucleolus 104 305 163 160 84 101 107 87 102 84 73 84 154 149 195 273 180 198 135 283 115 67 99 92
Peroxisomes 0 1 0 0 0 2 3 3 7 0 1 1 0 0 5 4 3 0 0 1 1 0 3 2
SpindlePole 4 89 108 111 64 185 234 188 147 170 99 115 110 110 136 58 46 55 11 80 83 26 58 61
Vac/Vac Membrane 11 15 4 0 3 11 11 15 9 7 5 5 1 0 4 10 2 8 9 23 10 9 18 16
Unique Cell Count 197 543 253 231 215 336 438 374 416 342 297 349 204 238 328 363 245 290 241 586 286 159 308 315
Labelled Cell Count 233 801 371 364 337 498 624 531 643 514 472 549 335 358 505 491 329 391 241 586 286 159 308 315


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 5.4 7.4 6.4 4.5 4.7 4.7 4.8 3.7 4.2 3.9 3.9 7.2 6.3 5.9 9.3 11.7 10.6 7.4 7.8 7.8
Std Deviation (1e-4) 1.4 1.0 2.0 1.6 1.2 1.1 1.4 1.0 0.9 1.1 1.0 0.8 1.7 1.8 1.7 3.0 3.1 3.5 1.8 1.6 1.8
Intensity Change (Log2) – – – -0.21 -0.72 -0.66 -0.65 -0.64 -0.99 -0.81 -0.93 -0.94 -0.05 -0.23 -0.34 0.32 0.65 0.51 0.0 0.06 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 2.1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 1.2 0.1 2.3 2.6 1.6 0.8 -0.4 2.1 2.3 3.8 4.8 0.3 0.3 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.6 0 0 0 0 0 0 0 0 1.6 1.1 3.9 -0.2 -0.4 -0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.1 0 0 0 0 0 0 0 0 0 0 0 3.6 2.7 1.4
Nucleus -0.5 2.4 -2.0 -2.5 -3.8 -3.5 -2.4 -1.9 -2.7 -3.7 -2.3 -2.2 -1.6 -0.6 0.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.1 0 0 0 0 0 0 0 0 2.6 -0.4 -1.2 2.9 2.2 0.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1.2 0 0 0 0 0 0 0 0 2.4 0.8 -0.3 -7.4 -5.8 -6.0
Vacuole 0 0 1.3 0.8 1.7 0 0 0 0 0 0 0 1.0 0 0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.7341 5.2377 4.7379 4.4268 4.703 4.7569 7.3622 6.8992 8.042 6.6405 7.1673 8.0419 5.6169 5.8687 5.1484 5.0766 5.4835 5.6314
Actin 0.0209 0.0001 0.0011 0.0004 0.0002 0.0019 0.0981 0.029 0 0.0211 0.0002 0.0001 0.009 0 0.0039 0.044 0.0015 0.0001
Bud 0.0004 0.0001 0.0002 0.0002 0.0001 0.0002 0.0009 0.0003 0.0001 0.0003 0.0003 0.0001 0.0003 0.0003 0.0001 0.0004 0.0003 0
Bud Neck 0.0025 0.0011 0.0012 0.0014 0.0008 0.0023 0.0022 0.0026 0.0008 0.0039 0.0017 0.0019 0.0018 0.0008 0.0009 0.0025 0.0015 0.0011
Bud Periphery 0.0008 0.0002 0.0003 0.001 0.0005 0.001 0.0032 0.0006 0.0001 0.0006 0.0013 0.0002 0.0007 0.0005 0.0001 0.0009 0.0014 0.0002
Bud Site 0.0041 0.0009 0.0015 0.001 0.0003 0.0007 0.0055 0.0425 0.001 0.0039 0.0003 0.0004 0.0022 0.0021 0.0005 0.0042 0.0011 0.0003
Cell Periphery 0.0005 0.0005 0.0005 0.0008 0.0003 0.0006 0.0004 0.0007 0.0004 0.001 0.0003 0.0003 0.0006 0.0003 0.0002 0.0006 0.0006 0.0002
Cytoplasm 0.0019 0.0003 0.0016 0.0001 0.0001 0.0001 0.0048 0.0018 0.0012 0.0002 0.0003 0 0.0039 0.0001 0.0002 0.0012 0.0034 0.0001
Cytoplasmic Foci 0.0296 0.0051 0.017 0.0031 0.0007 0.0044 0.0176 0.0069 0.017 0.0175 0.0302 0.0071 0.0225 0.0028 0.0058 0.0027 0.0071 0.0023
Eisosomes 0.0006 0 0.0001 0.0001 0.0001 0.0001 0.0009 0.0003 0 0.0001 0 0 0.0003 0 0.0001 0.0003 0.0001 0
Endoplasmic Reticulum 0.0013 0.0002 0.0078 0.0003 0.0002 0.0005 0.0045 0.0003 0.0001 0.0002 0.0006 0 0.0032 0.0001 0.0002 0.0038 0.0047 0.0003
Endosome 0.0129 0.0028 0.0082 0.0055 0.0008 0.0033 0.0425 0.0025 0.0119 0.0124 0.007 0.0017 0.0072 0.0006 0.0035 0.0035 0.0311 0.0019
Golgi 0.006 0.001 0.0082 0.0025 0.0007 0.0049 0.0087 0.0048 0.0008 0.0256 0.0018 0.0015 0.0035 0.0005 0.0014 0.0015 0.0078 0.0007
Lipid Particles 0.0868 0.0342 0.0462 0.047 0.0248 0.0245 0.0363 0.0329 0.0324 0.0745 0.106 0.0652 0.0325 0.0161 0.0297 0.0308 0.0484 0.0256
Mitochondria 0.0053 0.0004 0.0034 0.0249 0.0026 0.0138 0.0175 0.0055 0.0005 0.0035 0.0006 0.0007 0.0045 0.0002 0.0031 0.0062 0.0293 0.0016
None 0.0181 0.0002 0.0011 0.0002 0.0004 0.0002 0.0136 0.0007 0.0025 0.0003 0.0007 0.0001 0.0109 0.0001 0.0003 0.0012 0.001 0.0002
Nuclear Periphery 0.0216 0.0225 0.0131 0.0237 0.1088 0.0318 0.06 0.0197 0.0007 0.0043 0.058 0.0021 0.0226 0.0193 0.0079 0.0294 0.1141 0.0454
Nucleolus 0.5822 0.7355 0.7355 0.7552 0.6171 0.7273 0.5192 0.6601 0.8061 0.6433 0.6553 0.8168 0.7188 0.8004 0.7906 0.6206 0.4397 0.6824
Nucleus 0.0978 0.121 0.0984 0.0705 0.1596 0.1135 0.0336 0.0908 0.057 0.06 0.0811 0.0615 0.0886 0.1164 0.0916 0.1191 0.2023 0.1088
Peroxisomes 0.0159 0.0005 0.0066 0.0012 0.0004 0.0015 0.0095 0.0182 0.0011 0.0138 0.0012 0.0023 0.0055 0.0005 0.0007 0.0015 0.0021 0.0004
Punctate Nuclear 0.0852 0.0623 0.0365 0.0481 0.0769 0.0616 0.1116 0.0783 0.0592 0.1069 0.0411 0.0353 0.056 0.037 0.0576 0.1193 0.087 0.1255
Vacuole 0.0036 0.0076 0.0098 0.0077 0.0013 0.0029 0.0028 0.0006 0.0065 0.0054 0.0051 0.0018 0.0039 0.0013 0.0012 0.0031 0.0081 0.0014
Vacuole Periphery 0.0021 0.0034 0.0017 0.005 0.0035 0.003 0.0066 0.0009 0.0006 0.0013 0.0069 0.0009 0.0015 0.0006 0.0003 0.0033 0.0073 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.8533 35.1467 17.6674 16.4649 24.6657 10.1406 19.4879 11.2188 11.3083 22.3187
Translational Efficiency 1.6361 1.4821 2.0783 1.5732 1.8294 3.699 1.4872 1.9464 1.4175 1.7736

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative RNA exonuclease; possibly involved in pre-rRNA processing and ribosome assembly
Localization
Cell Percentages nucleolus (47%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-1

Rex4

Rex4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rex4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available