Standard name
Human Ortholog
Description Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0.05 0
Bud 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.08 0.08
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.27 0.32 0.26 0.19 0.19 0.11 0.15 0.15 0.18 0.55 0.6 0.53 0.59 0.47 0.52 0.34 0.15 0.34 0.3
Cytoplasm 0.92 0.97 0.91 0.9 0.87 0.9 0.79 0.76 0.76 0.91 0.89 0.94 0.64 0.73 0.65 0.43 0.71 0.45 0.49
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.13 0.17 0.17 0.29 0.36 0.32 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 13 2 3 6 2 3 10 6 1 10 9 5 34 17 34 22 4 12 16
Bud 9 9 9 15 11 9 21 24 19 11 6 11 6 2 7 2 0 1 2
Bud Neck 11 0 1 0 1 0 0 0 0 0 1 3 0 1 1 38 14 22 31
Bud Site 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
Cell Periphery 121 122 139 104 115 91 80 74 100 197 244 226 504 239 379 160 33 94 112
Cytoplasm 417 367 481 495 534 767 430 384 422 326 365 402 543 375 477 202 160 127 181
Endoplasmic Reticulum 4 1 0 5 4 2 6 3 4 1 4 3 41 19 25 15 2 10 11
Endosome 0 0 0 0 0 0 0 0 1 0 0 0 1 2 0 1 0 1 2
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 2 0 1 1 0 0 1
Mitochondria 2 17 33 70 102 142 161 182 176 0 0 1 20 1 9 19 1 3 4
Nucleus 0 1 0 1 0 3 3 0 1 0 0 2 16 10 15 0 0 0 0
Nuclear Periphery 0 1 0 0 0 2 0 0 2 1 0 3 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 1 0 1 0 0 1 1 0 0 0 6 2 8 0 0 0 0
Vac/Vac Membrane 0 0 5 2 6 5 0 5 0 0 1 0 32 13 27 3 3 3 4
Unique Cell Count 454 379 530 552 613 850 547 507 554 358 408 429 854 512 729 474 226 282 373
Labelled Cell Count 579 520 672 698 777 1025 711 679 728 546 630 656 1205 681 983 474 226 282 373


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.0 5.4 5.0 4.8 4.9 4.3 4.2 4.2 6.6 6.8 5.9 9.4 9.6 9.5 6.3 6.6 7.4
Std Deviation (1e-4) 0.7 1.0 1.2 1.1 0.9 0.8 0.7 0.7 0.7 0.9 0.9 0.8 1.2 1.4 1.5 1.1 1.2 1.2
Intensity Change (Log2) -0.05 0.05 -0.07 -0.11 -0.09 -0.28 -0.3 -0.32 0.34 0.39 0.17 0.85 0.88 0.86 0.28 0.34 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.5 -2.8 -2.1 0 -3.9 0 0 0 -0.1 -0.6 -1.8 1.0 0.4 1.5
Bud 0.4 -0.3 0.8 0 -1.4 0 0 0 1.0 -0.6 0.6 -2.1 -2.3 -1.5
Bud Neck -3.1 -3.2 0 0 0 0 0 0 0 -2.7 -2.0 0 -3.1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.8 -0.2 -3.0 0 -7.4 0 0 0 8.2 9.8 7.9 11.2 6.4 8.6
Cytoplasm 3.0 -0.6 -1.2 -2.5 -1.0 -5.8 -6.7 -6.6 -0.4 -1.2 1.1 -11.0 -7.5 -10.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 3.7 2.9 2.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.9 4.9 7.5 0 8.9 0 0 0 0 0 0 2.5 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 2.9 0 3.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 4.2 3.4 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8915 4.8872 4.0694 3.679 3.3801 4.048 1.1965 2.3336 1.9486 1.5183 1.6356 1.9086 1.8144 2.5145 1.8185 2.7123 2.2965 2.0943
Actin 0.0351 0.0009 0.0129 0.0157 0.0029 0.0032 0.0198 0.0023 0.0213 0.0539 0.0372 0.032 0.0524 0.001 0.0318 0.0486 0.0333 0.0128
Bud 0.0007 0.0001 0.0008 0.0008 0.0009 0.0005 0.0029 0.0004 0.0048 0.0005 0.003 0.0016 0.0044 0.0047 0.0116 0.0019 0.0299 0.0016
Bud Neck 0.0152 0.0015 0.0146 0.0094 0.0185 0.0175 0.0115 0.0048 0.0224 0.0142 0.0525 0.041 0.0052 0.0019 0.0103 0.0051 0.0206 0.0076
Bud Periphery 0.0031 0.0008 0.0051 0.0056 0.0083 0.0036 0.0088 0.0014 0.0106 0.0031 0.0053 0.0107 0.0065 0.0059 0.0139 0.0052 0.0297 0.0051
Bud Site 0.0166 0.031 0.0247 0.003 0.0045 0.0048 0.0148 0.1206 0.0646 0.0047 0.048 0.0024 0.0163 0.0908 0.0626 0.0322 0.0817 0.0013
Cell Periphery 0.1052 0.0786 0.1201 0.0869 0.1337 0.0678 0.158 0.1235 0.1383 0.1905 0.0571 0.0931 0.0637 0.0488 0.0706 0.0525 0.0345 0.0291
Cytoplasm 0.596 0.7762 0.6325 0.7026 0.687 0.815 0.6509 0.715 0.6631 0.6323 0.6982 0.7723 0.5419 0.766 0.6422 0.7079 0.4833 0.8301
Cytoplasmic Foci 0.0208 0.0006 0.004 0.0017 0.0016 0.0013 0.037 0.0007 0.0044 0.0036 0.024 0.0163 0.0231 0.0011 0.0059 0.015 0.0136 0.0041
Eisosomes 0.0021 0.0004 0.0023 0.0009 0.0007 0.0002 0.0005 0.0005 0.0011 0.0005 0.0005 0.0004 0.0011 0.0005 0.0011 0.0003 0.0007 0.0002
Endoplasmic Reticulum 0.0099 0.0017 0.003 0.0054 0.0086 0.0023 0.0047 0.0022 0.0061 0.0036 0.0102 0.0039 0.0126 0.0022 0.0071 0.0034 0.0069 0.0041
Endosome 0.011 0.0001 0.0009 0.0003 0.0036 0.0005 0.0112 0.0001 0.0042 0.0006 0.0067 0.0018 0.0314 0.0001 0.0065 0.0077 0.0526 0.0039
Golgi 0.008 0.0001 0.0013 0.0004 0.0004 0.0006 0.0053 0.0001 0.0049 0.0026 0.0055 0.0018 0.0123 0.0001 0.0043 0.0093 0.0229 0.005
Lipid Particles 0.0085 0.0003 0.003 0.001 0.0021 0.0019 0.0076 0.0003 0.0037 0.0019 0.002 0.0016 0.0209 0.0005 0.0062 0.004 0.0339 0.0017
Mitochondria 0.0032 0.0001 0.0019 0.0002 0.0004 0.0002 0.0031 0 0.0024 0.0022 0.0026 0.0005 0.0121 0 0.0236 0.0022 0.0126 0.0037
None 0.1184 0.106 0.1601 0.1627 0.0847 0.0759 0.024 0.0262 0.0398 0.0781 0.0167 0.0167 0.1391 0.0739 0.07 0.0901 0.1109 0.0826
Nuclear Periphery 0.0071 0.0001 0.0002 0.0004 0.0109 0.0002 0.0043 0.0001 0.0006 0.0001 0.0039 0.0004 0.0131 0.0002 0.0051 0.0008 0.0035 0.001
Nucleolus 0.0022 0.0001 0.0003 0.0002 0.0013 0.0001 0.0025 0.0001 0.0002 0.0001 0.0007 0.0002 0.0025 0.0002 0.0032 0.0013 0.0039 0.0002
Nucleus 0.0081 0.0003 0.0005 0.0008 0.0205 0.0003 0.0043 0.0004 0.0008 0.0003 0.0157 0.0004 0.0138 0.0005 0.0083 0.0014 0.0049 0.0009
Peroxisomes 0.0129 0 0.0092 0.0001 0.0001 0.0029 0.0113 0 0.0038 0.0056 0.0046 0.0007 0.0089 0 0.0032 0.0039 0.0059 0.0006
Punctate Nuclear 0.0105 0 0.0004 0.0004 0.0033 0.0003 0.0125 0.0001 0.0006 0.0001 0.0014 0.0011 0.0137 0.0003 0.0049 0.0041 0.0063 0.0009
Vacuole 0.0044 0.0011 0.0019 0.0014 0.0056 0.0009 0.0042 0.0011 0.0023 0.0012 0.0036 0.001 0.0041 0.0013 0.0062 0.003 0.0068 0.0034
Vacuole Periphery 0.0006 0 0.0001 0 0.0004 0 0.0006 0 0.0002 0 0.0007 0.0001 0.0011 0 0.0015 0.0002 0.0015 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.3965 13.2459 12.2317 16.936 11.7403 17.442 16.9643 23.2009 20.866 18.4688
Translational Efficiency 0.6889 0.3838 0.4872 0.3634 0.3361 0.3929 0.4295 0.3122 0.416 0.3414

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid
Localization
Cell Percentages cytoplasm (70%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Duf1

Duf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Duf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available