Standard name | |
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Human Ortholog | |
Description | Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | – | 13 | 2 | 3 | 6 | 2 | 3 | 10 | – | 6 | 1 | 10 | 9 | 5 | 34 | 17 | 34 | – | – | 22 | 4 | 12 | 16 |
Bud | – | – | 9 | 9 | 9 | 15 | 11 | 9 | 21 | – | 24 | 19 | 11 | 6 | 11 | 6 | 2 | 7 | – | – | 2 | 0 | 1 | 2 |
Bud Neck | – | – | 11 | 0 | 1 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 1 | – | – | 38 | 14 | 22 | 31 |
Bud Site | – | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | – | 121 | 122 | 139 | 104 | 115 | 91 | 80 | – | 74 | 100 | 197 | 244 | 226 | 504 | 239 | 379 | – | – | 160 | 33 | 94 | 112 |
Cytoplasm | – | – | 417 | 367 | 481 | 495 | 534 | 767 | 430 | – | 384 | 422 | 326 | 365 | 402 | 543 | 375 | 477 | – | – | 202 | 160 | 127 | 181 |
Endoplasmic Reticulum | – | – | 4 | 1 | 0 | 5 | 4 | 2 | 6 | – | 3 | 4 | 1 | 4 | 3 | 41 | 19 | 25 | – | – | 15 | 2 | 10 | 11 |
Endosome | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | – | – | 1 | 0 | 1 | 2 |
Golgi | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | – | – | 1 | 0 | 0 | 1 |
Mitochondria | – | – | 2 | 17 | 33 | 70 | 102 | 142 | 161 | – | 182 | 176 | 0 | 0 | 1 | 20 | 1 | 9 | – | – | 19 | 1 | 3 | 4 |
Nucleus | – | – | 0 | 1 | 0 | 1 | 0 | 3 | 3 | – | 0 | 1 | 0 | 0 | 2 | 16 | 10 | 15 | – | – | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | – | 0 | 1 | 0 | 0 | 0 | 2 | 0 | – | 0 | 2 | 1 | 0 | 3 | 0 | 0 | 0 | – | – | 0 | 0 | 1 | 0 |
Nucleolus | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Peroxisomes | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
SpindlePole | – | – | 1 | 0 | 1 | 0 | 1 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 6 | 2 | 8 | – | – | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | – | 0 | 0 | 5 | 2 | 6 | 5 | 0 | – | 5 | 0 | 0 | 1 | 0 | 32 | 13 | 27 | – | – | 3 | 3 | 3 | 4 |
Unique Cell Count | 454 | 379 | 530 | 552 | 613 | 850 | 547 | 507 | 554 | 358 | 408 | 429 | 854 | 512 | 729 | 474 | 226 | 282 | 373 | |||||
Labelled Cell Count | 579 | 520 | 672 | 698 | 777 | 1025 | 711 | 679 | 728 | 546 | 630 | 656 | 1205 | 681 | 983 | 474 | 226 | 282 | 373 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | – | 5.2 | 5.0 | 5.4 | 5.0 | 4.8 | 4.9 | 4.3 | – | 4.2 | 4.2 | 6.6 | 6.8 | 5.9 | 9.4 | 9.6 | 9.5 | 6.3 | 6.6 | 7.4 |
Std Deviation (1e-4) | – | – | 0.7 | 1.0 | 1.2 | 1.1 | 0.9 | 0.8 | 0.7 | – | 0.7 | 0.7 | 0.9 | 0.9 | 0.8 | 1.2 | 1.4 | 1.5 | 1.1 | 1.2 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.05 | -0.07 | -0.11 | -0.09 | -0.28 | – | -0.3 | -0.32 | 0.34 | 0.39 | 0.17 | 0.85 | 0.88 | 0.86 | 0.28 | 0.34 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.5 | -2.8 | -2.1 | 0 | -3.9 | 0 | – | 0 | 0 | -0.1 | -0.6 | -1.8 | 1.0 | 0.4 | 1.5 |
Bud | 0.4 | -0.3 | 0.8 | 0 | -1.4 | 0 | – | 0 | 0 | 1.0 | -0.6 | 0.6 | -2.1 | -2.3 | -1.5 |
Bud Neck | -3.1 | -3.2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -2.7 | -2.0 | 0 | -3.1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.8 | -0.2 | -3.0 | 0 | -7.4 | 0 | – | 0 | 0 | 8.2 | 9.8 | 7.9 | 11.2 | 6.4 | 8.6 |
Cytoplasm | 3.0 | -0.6 | -1.2 | -2.5 | -1.0 | -5.8 | – | -6.7 | -6.6 | -0.4 | -1.2 | 1.1 | -11.0 | -7.5 | -10.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.7 | 2.9 | 2.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.9 | 4.9 | 7.5 | 0 | 8.9 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 3.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4.2 | 3.4 | 4.1 |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.8915 | 4.8872 | 4.0694 | 3.679 | 3.3801 | 4.048 | 1.1965 | 2.3336 | 1.9486 | 1.5183 | 1.6356 | 1.9086 | 1.8144 | 2.5145 | 1.8185 | 2.7123 | 2.2965 | 2.0943 |
Actin | 0.0351 | 0.0009 | 0.0129 | 0.0157 | 0.0029 | 0.0032 | 0.0198 | 0.0023 | 0.0213 | 0.0539 | 0.0372 | 0.032 | 0.0524 | 0.001 | 0.0318 | 0.0486 | 0.0333 | 0.0128 |
Bud | 0.0007 | 0.0001 | 0.0008 | 0.0008 | 0.0009 | 0.0005 | 0.0029 | 0.0004 | 0.0048 | 0.0005 | 0.003 | 0.0016 | 0.0044 | 0.0047 | 0.0116 | 0.0019 | 0.0299 | 0.0016 |
Bud Neck | 0.0152 | 0.0015 | 0.0146 | 0.0094 | 0.0185 | 0.0175 | 0.0115 | 0.0048 | 0.0224 | 0.0142 | 0.0525 | 0.041 | 0.0052 | 0.0019 | 0.0103 | 0.0051 | 0.0206 | 0.0076 |
Bud Periphery | 0.0031 | 0.0008 | 0.0051 | 0.0056 | 0.0083 | 0.0036 | 0.0088 | 0.0014 | 0.0106 | 0.0031 | 0.0053 | 0.0107 | 0.0065 | 0.0059 | 0.0139 | 0.0052 | 0.0297 | 0.0051 |
Bud Site | 0.0166 | 0.031 | 0.0247 | 0.003 | 0.0045 | 0.0048 | 0.0148 | 0.1206 | 0.0646 | 0.0047 | 0.048 | 0.0024 | 0.0163 | 0.0908 | 0.0626 | 0.0322 | 0.0817 | 0.0013 |
Cell Periphery | 0.1052 | 0.0786 | 0.1201 | 0.0869 | 0.1337 | 0.0678 | 0.158 | 0.1235 | 0.1383 | 0.1905 | 0.0571 | 0.0931 | 0.0637 | 0.0488 | 0.0706 | 0.0525 | 0.0345 | 0.0291 |
Cytoplasm | 0.596 | 0.7762 | 0.6325 | 0.7026 | 0.687 | 0.815 | 0.6509 | 0.715 | 0.6631 | 0.6323 | 0.6982 | 0.7723 | 0.5419 | 0.766 | 0.6422 | 0.7079 | 0.4833 | 0.8301 |
Cytoplasmic Foci | 0.0208 | 0.0006 | 0.004 | 0.0017 | 0.0016 | 0.0013 | 0.037 | 0.0007 | 0.0044 | 0.0036 | 0.024 | 0.0163 | 0.0231 | 0.0011 | 0.0059 | 0.015 | 0.0136 | 0.0041 |
Eisosomes | 0.0021 | 0.0004 | 0.0023 | 0.0009 | 0.0007 | 0.0002 | 0.0005 | 0.0005 | 0.0011 | 0.0005 | 0.0005 | 0.0004 | 0.0011 | 0.0005 | 0.0011 | 0.0003 | 0.0007 | 0.0002 |
Endoplasmic Reticulum | 0.0099 | 0.0017 | 0.003 | 0.0054 | 0.0086 | 0.0023 | 0.0047 | 0.0022 | 0.0061 | 0.0036 | 0.0102 | 0.0039 | 0.0126 | 0.0022 | 0.0071 | 0.0034 | 0.0069 | 0.0041 |
Endosome | 0.011 | 0.0001 | 0.0009 | 0.0003 | 0.0036 | 0.0005 | 0.0112 | 0.0001 | 0.0042 | 0.0006 | 0.0067 | 0.0018 | 0.0314 | 0.0001 | 0.0065 | 0.0077 | 0.0526 | 0.0039 |
Golgi | 0.008 | 0.0001 | 0.0013 | 0.0004 | 0.0004 | 0.0006 | 0.0053 | 0.0001 | 0.0049 | 0.0026 | 0.0055 | 0.0018 | 0.0123 | 0.0001 | 0.0043 | 0.0093 | 0.0229 | 0.005 |
Lipid Particles | 0.0085 | 0.0003 | 0.003 | 0.001 | 0.0021 | 0.0019 | 0.0076 | 0.0003 | 0.0037 | 0.0019 | 0.002 | 0.0016 | 0.0209 | 0.0005 | 0.0062 | 0.004 | 0.0339 | 0.0017 |
Mitochondria | 0.0032 | 0.0001 | 0.0019 | 0.0002 | 0.0004 | 0.0002 | 0.0031 | 0 | 0.0024 | 0.0022 | 0.0026 | 0.0005 | 0.0121 | 0 | 0.0236 | 0.0022 | 0.0126 | 0.0037 |
None | 0.1184 | 0.106 | 0.1601 | 0.1627 | 0.0847 | 0.0759 | 0.024 | 0.0262 | 0.0398 | 0.0781 | 0.0167 | 0.0167 | 0.1391 | 0.0739 | 0.07 | 0.0901 | 0.1109 | 0.0826 |
Nuclear Periphery | 0.0071 | 0.0001 | 0.0002 | 0.0004 | 0.0109 | 0.0002 | 0.0043 | 0.0001 | 0.0006 | 0.0001 | 0.0039 | 0.0004 | 0.0131 | 0.0002 | 0.0051 | 0.0008 | 0.0035 | 0.001 |
Nucleolus | 0.0022 | 0.0001 | 0.0003 | 0.0002 | 0.0013 | 0.0001 | 0.0025 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0.0025 | 0.0002 | 0.0032 | 0.0013 | 0.0039 | 0.0002 |
Nucleus | 0.0081 | 0.0003 | 0.0005 | 0.0008 | 0.0205 | 0.0003 | 0.0043 | 0.0004 | 0.0008 | 0.0003 | 0.0157 | 0.0004 | 0.0138 | 0.0005 | 0.0083 | 0.0014 | 0.0049 | 0.0009 |
Peroxisomes | 0.0129 | 0 | 0.0092 | 0.0001 | 0.0001 | 0.0029 | 0.0113 | 0 | 0.0038 | 0.0056 | 0.0046 | 0.0007 | 0.0089 | 0 | 0.0032 | 0.0039 | 0.0059 | 0.0006 |
Punctate Nuclear | 0.0105 | 0 | 0.0004 | 0.0004 | 0.0033 | 0.0003 | 0.0125 | 0.0001 | 0.0006 | 0.0001 | 0.0014 | 0.0011 | 0.0137 | 0.0003 | 0.0049 | 0.0041 | 0.0063 | 0.0009 |
Vacuole | 0.0044 | 0.0011 | 0.0019 | 0.0014 | 0.0056 | 0.0009 | 0.0042 | 0.0011 | 0.0023 | 0.0012 | 0.0036 | 0.001 | 0.0041 | 0.0013 | 0.0062 | 0.003 | 0.0068 | 0.0034 |
Vacuole Periphery | 0.0006 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0006 | 0 | 0.0002 | 0 | 0.0007 | 0.0001 | 0.0011 | 0 | 0.0015 | 0.0002 | 0.0015 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.3965 | 13.2459 | 12.2317 | 16.936 | 11.7403 | 17.442 | 16.9643 | 23.2009 | 20.866 | 18.4688 |
Translational Efficiency | 0.6889 | 0.3838 | 0.4872 | 0.3634 | 0.3361 | 0.3929 | 0.4295 | 0.3122 | 0.416 | 0.3414 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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