Standard name
Human Ortholog
Description Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0 0 0 0 0 0 0.06 0.13 0.17 0.06 0.06 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.25 0.41 0.63 0.57 0.36 0.69 0.7 0.63 0.87 0.85 0.84 0.84 0 0 0.12 0.07 0.06 0.1 0 0 0 0 0 0
Nucleus 0.85 0.81 0.74 0.79 0.8 0.55 0.52 0.56 0.45 0.46 0.51 0.48 0.92 0.84 0.72 0.81 0.84 0.79 0.74 0.77 0.66 0.84 0.72 0.54
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.06 0 0 0.05 0.06 0.08 0.08 0.06 0.09 0.08 0.12 0 0 0 0 0 0 0.12 0.07 0.08 0 0.08 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.08 0 0 0.14 0 0.12 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2
Bud 2 0 0 0 1 5 2 17 5 11 9 10 1 0 2 1 0 0 0 0 0 0 5 3
Bud Neck 0 0 0 0 0 1 1 2 0 1 0 1 0 0 0 0 0 0 0 0 1 0 0 2
Bud Site 0 0 0 0 0 8 20 21 6 18 12 21 0 0 3 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 0 2 10 4 9 9 24 13 15 9 20 17 67 110 14 14 32 2 0 0 1 3 1
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 0 1 1 2 10 6 12 0 0 0 0 1 3
Endosome 0 0 0 0 2 2 4 4 1 0 0 0 0 2 1 11 2 12 2 1 1 1 7 27
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 2
Mitochondria 39 22 59 118 104 216 356 427 268 435 206 457 6 14 75 15 14 40 0 3 1 0 4 12
Nucleus 135 44 70 165 231 172 267 383 138 238 125 260 241 438 453 181 198 325 125 58 66 110 339 357
Nuclear Periphery 3 0 0 0 2 2 5 3 1 6 2 6 0 2 6 2 1 0 4 0 0 0 0 4
Nucleolus 7 3 1 4 14 19 43 56 19 44 19 67 0 8 13 3 3 4 20 5 8 5 37 77
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 7 6 29 46 6 18 7 11 0 6 2 2 3 10 5 1 4 4 8 20
Vac/Vac Membrane 2 1 0 0 2 6 6 5 5 3 0 3 3 7 20 21 17 34 7 2 14 5 57 134
Unique Cell Count 159 54 94 208 290 311 509 678 308 514 244 541 262 521 629 224 237 413 171 76 100 132 471 662
Labelled Cell Count 193 70 133 298 368 446 742 988 462 789 389 857 269 546 689 261 259 470 171 76 100 132 471 662


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.2 3.5 4.0 4.1 3.7 3.4 3.5 3.0 3.1 3.1 2.9 5.6 5.4 4.8 6.5 7.0 7.5 5.2 5.2 5.6
Std Deviation (1e-4) 0.6 0.6 0.5 1.2 1.0 1.0 0.8 0.8 0.9 0.8 0.9 0.9 1.0 1.2 1.0 1.4 1.3 1.7 1.0 1.1 1.1
Intensity Change (Log2) – – – 0.17 0.22 0.06 -0.05 -0.0 -0.23 -0.19 -0.2 -0.27 0.67 0.63 0.45 0.9 1.0 1.09 0.57 0.58 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – 0
Bud Neck – – – – – – – – – – – – 0 – 0
Bud Site – – – – – – – – – – – – 0 – 0
Cell Periphery – – – – – – – – – – – – 0 – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – 0
Endosome – – – – – – – – – – – – 0 – 0
Golgi – – – – – – – – – – – – 0 – 0
Mitochondria – – – – – – – – – – – – 0 – 0
Nuclear Periphery – – – – – – – – – – – – 0 – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – 0
Peroxisomes – – – – – – – – – – – – 0 – 0
SpindlePole – – – – – – – – – – – – 0 – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – 0
Cytoplasm – – – – – – – – – – – – 0 – 0
Nucleus – – – – – – – – – – – – 0 – 0
Vacuole – – – – – – – – – – – – 3.1 – 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4492 1.7947 1.7296 1.3065 1.0972 1.7424 1.9941 2.1403 1.9943 1.7698 2.2038 1.9101 0.4384 0.7638 -0.0368 0.7638 0.2049 0.1039
Actin 0.0438 0.0004 0.0062 0.0002 0.0202 0.0087 0.0369 0.0019 0.0287 0.0124 0.0033 0.0035 0.0121 0.0002 0.0001 0.0002 0.0002 0
Bud 0.001 0.0002 0.0002 0.0001 0.0009 0.0002 0.0008 0.0002 0.0002 0.0005 0.0003 0.0002 0.0002 0.0001 0 0.0001 0 0.0001
Bud Neck 0.0133 0.0025 0.0013 0.001 0.0035 0.0062 0.0047 0.0012 0.0007 0.0016 0.0054 0.0023 0.0007 0.0005 0.0005 0.0007 0.0007 0.0037
Bud Periphery 0.003 0.0005 0.0004 0.0003 0.0031 0.0007 0.0014 0.0006 0.0004 0.0007 0.0011 0.0005 0.0003 0.0003 0.0001 0.0002 0.0001 0.0001
Bud Site 0.0073 0.0103 0.0007 0.0002 0.0135 0.0005 0.0034 0.0031 0.0013 0.0022 0.0011 0.0003 0.0015 0.0007 0.0003 0.0004 0.0002 0.0003
Cell Periphery 0.0009 0.0003 0.0001 0.0001 0.0012 0.0001 0.0002 0.0002 0.0001 0.0002 0.0004 0.0001 0.0001 0.0001 0 0.0001 0 0.0001
Cytoplasm 0.0041 0.0057 0.0013 0.0006 0.0008 0.0007 0.0033 0.0046 0.0023 0.0019 0.0022 0.0001 0.0049 0.0016 0.0012 0.0031 0.0042 0.0006
Cytoplasmic Foci 0.0209 0.0008 0.0102 0.0001 0.004 0.0007 0.0276 0.0036 0.0042 0.0091 0.0086 0.0008 0.0026 0.0001 0.0001 0.0001 0.0003 0.0002
Eisosomes 0.0008 0.0001 0.0001 0 0.0003 0.0001 0.0002 0 0.0002 0.0001 0.0001 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.005 0.0014 0.0021 0.0002 0.0008 0.0009 0.0022 0.0005 0.0033 0.0013 0.0065 0.0002 0.0009 0.0003 0.0001 0.0001 0.0006 0.0001
Endosome 0.0266 0.0013 0.0131 0.0003 0.0044 0.0042 0.0146 0.0016 0.0111 0.0192 0.042 0.0028 0.0092 0.0005 0.0003 0.0004 0.0008 0.0003
Golgi 0.0099 0.0002 0.006 0 0.0029 0.002 0.011 0.0015 0.005 0.0167 0.011 0.0037 0.0019 0 0 0.0001 0 0
Lipid Particles 0.0322 0.0011 0.0192 0.0001 0.0091 0.0017 0.0492 0.0014 0.0073 0.0048 0.0326 0.005 0.003 0.0002 0.0001 0.0003 0.0001 0.0001
Mitochondria 0.0145 0.0006 0.0039 0.0049 0.0252 0.0062 0.006 0.0022 0.018 0.0113 0.0851 0.0012 0.0011 0.001 0.0003 0.0015 0.0005 0.0001
None 0.0025 0.0016 0.0016 0.0006 0.0007 0.0007 0.001 0.0007 0.0005 0.0008 0.0006 0.0001 0.0084 0.0133 0.0239 0.013 0.0282 0.0007
Nuclear Periphery 0.0649 0.0354 0.065 0.0155 0.0352 0.0209 0.0469 0.0175 0.0219 0.0124 0.0685 0.0022 0.0144 0.0345 0.021 0.0149 0.0212 0.0062
Nucleolus 0.0777 0.0933 0.1265 0.1544 0.1546 0.1562 0.0434 0.0588 0.0851 0.0735 0.0588 0.1529 0.0546 0.0768 0.0658 0.1744 0.0697 0.1044
Nucleus 0.6218 0.7929 0.6861 0.7647 0.6829 0.7616 0.7042 0.8899 0.7807 0.7486 0.6494 0.8158 0.8573 0.8335 0.863 0.7699 0.8211 0.86
Peroxisomes 0.0087 0.0002 0.014 0.0001 0.0074 0.0004 0.0253 0.0017 0.007 0.023 0.002 0.0008 0.0016 0 0 0.0001 0 0
Punctate Nuclear 0.0306 0.0487 0.0381 0.0554 0.0174 0.0258 0.0124 0.0063 0.0174 0.0569 0.0103 0.0069 0.0231 0.0343 0.0226 0.0197 0.0513 0.0226
Vacuole 0.0063 0.0021 0.0018 0.0005 0.0076 0.0007 0.0029 0.0016 0.0031 0.0016 0.0077 0.0003 0.0013 0.001 0.0003 0.0004 0.0005 0.0004
Vacuole Periphery 0.0043 0.0006 0.0023 0.0008 0.0044 0.0008 0.0023 0.0008 0.0014 0.0012 0.0029 0.0003 0.0008 0.0011 0.0002 0.0003 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.2753 9.8861 4.7328 13.5412 8.5568 7.844 12.1723 11.8608 11.1329 14.1298
Translational Efficiency 0.7434 0.8136 1.5746 0.5021 0.8784 0.9826 0.8545 0.6512 0.7664 0.638

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Hal9

Hal9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hal9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available