Standard name
Human Ortholog
Description Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter; YPQ1 has a paralog, RTC2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0 0 0 0.06 0.11 0.15 0.16 0.26 0.23 0.27 0.34 0 0 0 0.11 0.07 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.07 0 0 0.08 0.06 0.06 0.05 0.05 0.09 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.1 0.11 0.08 0 0.05 0.11 0.07 0.05 0.05 0.05 0 0 0.05 0 0.06 0.21 0.21 0.21 0 0.07 0.05 0.07 0.07 0.06
Nuclear Periphery 0.21 0 0.13 0.28 0.16 0.12 0.17 0.1 0.12 0.07 0.09 0.06 0.07 0.13 0.09 0.08 0.08 0.06 0 0 0.06 0 0 0
Nucleolus 0.08 0 0.08 0.09 0.05 0 0 0 0 0 0 0 0.1 0.08 0 0 0.08 0 0 0 0 0 0.05 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.62 0.88 0.8 0.84 0.81 0.7 0.7 0.72 0.66 0.66 0.64 0.59 0.84 0.88 0.93 0.75 0.76 0.77 0.83 0.87 0.84 0.79 0.81 0.79
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 3 2 0 1 0 2 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 0 0 0 1 2 5 1 4 4 3 0 0 0 0 0 0 0 2 1 1 3 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 0 1 1 4 13 7 8 11 6 5 0 1 1 0 1 0 0 0 0 0 0 0
Cytoplasm 5 11 9 4 26 50 100 100 107 124 85 147 7 9 6 13 5 8 1 1 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 3 3 5 11 9 11 4 9 0 1 2 4 4 0 0 0 0 0 0 0
Endosome 0 0 1 4 2 2 9 2 3 5 1 2 1 2 1 0 0 0 1 4 3 4 6 6
Golgi 2 8 2 10 4 0 0 1 0 1 1 0 9 3 6 0 0 0 0 0 0 1 0 3
Mitochondria 0 6 19 7 19 35 42 38 20 28 28 41 5 3 0 0 1 0 0 1 2 2 1 5
Nucleus 4 49 21 5 22 48 46 33 19 29 11 17 14 14 22 25 15 14 2 34 13 14 20 20
Nuclear Periphery 8 8 34 65 70 52 114 65 48 38 27 24 19 41 36 10 6 4 2 5 16 8 5 7
Nucleolus 3 16 21 22 22 9 14 15 3 8 3 3 28 27 11 1 6 0 1 7 6 8 14 16
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 1 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 24 389 212 196 347 307 462 453 274 361 199 255 234 279 356 91 55 51 55 420 244 163 237 263
Unique Cell Count 39 442 265 232 427 440 662 627 414 543 310 435 277 318 384 121 72 66 67 485 292 207 292 334
Labelled Cell Count 49 488 319 314 516 512 811 732 492 622 369 508 318 381 441 144 93 77 67 485 292 207 292 334


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 22.9 31.9 20.6 21.4 15.8 12.3 11.5 10.4 10.5 9.6 9.7 9.5 29.9 28.2 23.1 35.1 36.4 36.6 31.5 33.6 32.6
Std Deviation (1e-4) 8.3 10.7 7.7 6.2 5.2 5.0 4.8 4.1 4.0 3.9 3.9 3.7 10.6 8.6 7.3 17.5 17.1 18.1 11.6 11.9 12.6
Intensity Change (Log2) – – – 0.06 -0.38 -0.74 -0.84 -0.98 -0.96 -1.11 -1.08 -1.11 0.54 0.45 0.17 0.77 0.82 0.83 0.61 0.71 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.2 1.6 3.7 5.0 5.2 7.6 7.0 7.8 9.4 -0.6 -0.4 -1.5 2.9 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 2.6 0 0 0 0 0 0 0 0 2.1 0 0 0 0 0
Mitochondria -2.1 -1.5 0.4 -0.5 -0.6 -1.3 -1.1 0.8 1.0 -3.0 -3.9 -5.3 -3.0 0 0
Nucleus -2.9 -1.5 1.3 -0.5 -1.5 -1.8 -1.4 -2.3 -2.3 -1.4 -1.8 -1.1 3.6 3.1 3.1
Nuclear Periphery 4.2 1.3 -0.4 1.6 -1.1 -0.5 -2.7 -1.6 -3.4 -2.3 0 -1.4 -1.3 -1.0 -1.5
Nucleolus 0.6 -1.5 -3.7 -4.2 -3.8 -5.0 -4.6 -4.2 -5.1 0.9 0.2 -2.9 -2.8 0.1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.3 0.4 -3.0 -3.2 -2.4 -3.9 -4.0 -4.2 -5.8 1.4 2.6 4.8 -1.1 -0.7 -0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 21.4792 28.5049 25.9696 23.3663 18.8786 25.3692 22.5592 31.3427 27.828 22.7434 21.0128 26.6092 23.6645 32.117 26.6341 26.4825 23.8745 27.9348
Actin 0.0002 0 0.0012 0 0.0018 0.0001 0.0006 0.0003 0.003 0.0337 0.0331 0.0106 0.0032 0 0 0 0 0
Bud 0.0001 0 0.0001 0 0.0002 0 0.0004 0 0.0003 0.0019 0.0024 0.0002 0.0001 0 0.0003 0 0 0
Bud Neck 0 0 0 0 0.0001 0 0.0002 0 0.0001 0.0004 0.001 0.0001 0.0002 0 0.0001 0 0 0
Bud Periphery 0.0003 0.0001 0.0002 0.0001 0.0005 0.0001 0.0013 0.0001 0.0011 0.0034 0.0036 0.0008 0.0002 0.0001 0.0007 0.0001 0.0001 0.0001
Bud Site 0.0003 0 0.0001 0 0.0002 0 0.0009 0.0001 0.0002 0.0014 0.024 0.0002 0.0001 0 0.0001 0 0 0
Cell Periphery 0.003 0.0025 0.0033 0.0023 0.0029 0.0014 0.0044 0.0051 0.003 0.0028 0.0016 0.0055 0.0039 0.0021 0.0027 0.0028 0.0009 0.0007
Cytoplasm 0.0003 0.0029 0.0002 0.0001 0.0017 0.0004 0.0045 0.0001 0.0007 0.0009 0.0071 0.0001 0.0004 0.0001 0.0001 0 0.0001 0.0001
Cytoplasmic Foci 0.0045 0 0.0005 0 0.0048 0 0.0033 0.0033 0.0005 0.016 0.0076 0.0005 0.0003 0 0 0 0 0
Eisosomes 0.0001 0 0 0 0 0 0 0 0.0002 0.0003 0.0005 0.0001 0 0 0 0 0 0
Endoplasmic Reticulum 0.0001 0 0.0002 0 0.0004 0.0002 0.0008 0 0.002 0.0014 0.0071 0.0006 0.0002 0 0 0 0 0
Endosome 0.0019 0.0006 0.0019 0.0008 0.0066 0.0014 0.0066 0.0017 0.0038 0.011 0.0216 0.0031 0.0018 0.0007 0.0013 0.0008 0.0008 0.0007
Golgi 0.0002 0 0.0004 0 0.0013 0 0.0006 0.002 0.003 0.0085 0.0055 0.0025 0.0002 0 0 0 0 0
Lipid Particles 0.0029 0.0001 0.0009 0.0007 0.0075 0.0002 0.005 0.002 0.0023 0.0179 0.0073 0.0051 0.001 0.0002 0.0007 0.0005 0.0003 0.0004
Mitochondria 0.0104 0.0001 0.0043 0.0066 0.004 0.0004 0.0188 0.002 0.005 0.0197 0.0087 0.0029 0.0065 0.0012 0.0017 0.0003 0.0002 0.0002
None 0.0008 0 0.0001 0 0.0006 0.0001 0.0019 0 0.0007 0.0018 0.0067 0.0002 0.0008 0 0 0 0 0
Nuclear Periphery 0.002 0.0053 0.0016 0.0006 0.0033 0.0065 0.003 0.0005 0.0037 0.0024 0.0077 0.0074 0.0009 0.001 0.0014 0.0003 0.0005 0.0023
Nucleolus 0.0119 0.0012 0.0101 0.0095 0.0037 0.0046 0.0191 0.0063 0.0016 0.0086 0.0014 0.0093 0.0084 0.0026 0.0025 0.0012 0.0006 0.0013
Nucleus 0.0052 0.0032 0.0101 0.0037 0.0048 0.0091 0.0107 0.0024 0.0013 0.0032 0.0036 0.0021 0.0031 0.0036 0.0032 0.0006 0.0005 0.0102
Peroxisomes 0.0004 0 0.0003 0 0.0035 0 0.0007 0.0033 0.0002 0.0034 0.003 0.0004 0.0001 0 0.0001 0 0 0
Punctate Nuclear 0.0005 0 0.0003 0 0.0046 0.0004 0.0011 0.0001 0.0001 0.0017 0.007 0.0003 0.0004 0 0 0 0 0
Vacuole 0.3119 0.3759 0.3496 0.3808 0.3715 0.2932 0.2811 0.2933 0.2927 0.3282 0.2917 0.2733 0.3121 0.3184 0.3255 0.3465 0.2819 0.264
Vacuole Periphery 0.6428 0.6082 0.6145 0.5946 0.576 0.6816 0.6348 0.6773 0.6745 0.5315 0.5479 0.6749 0.6561 0.6697 0.6595 0.6468 0.714 0.72

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 66.6566 61.715 58.4284 67.4242 61.7308 66.6197 62.9379 46.4726 50.0328 72.0783
Translational Efficiency 1.6309 1.5295 1.5298 1.2283 1.2794 1.4464 1.2777 1.2276 1.2014 1.0499

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter; YPQ1 has a paralog, RTC2, that arose from the whole genome duplication
Localization
Cell Percentages vacuolar membrane (74%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Ypq1

Ypq1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypq1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available