Standard name | |
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Human Ortholog | |
Description | Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway; involved in maintenance of cell wall integrity; involved in response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis; WSC3 has a paralog, WSC2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 28 | 35 | 9 | 18 | 5 | 5 | 2 | 2 | – | 0 | 0 | 2 | 38 | 25 | 19 | – | – | 45 | 46 | 56 | 39 | 48 | 77 | 48 |
Bud | 10 | 10 | 6 | 8 | 14 | 14 | 17 | 9 | – | 19 | 19 | 18 | 3 | 9 | 5 | – | – | 13 | 9 | 13 | 4 | 9 | 12 | 11 |
Bud Neck | 6 | 1 | 1 | 2 | 6 | 2 | 1 | 4 | – | 5 | 0 | 3 | 23 | 22 | 18 | – | – | 2 | 34 | 27 | 9 | 15 | 30 | 28 |
Bud Site | 3 | 7 | 4 | 5 | 3 | 7 | 3 | 1 | – | 4 | 0 | 2 | 6 | 0 | 2 | – | – | 5 | – | – | – | – | – | – |
Cell Periphery | 21 | 11 | 15 | 17 | 9 | 9 | 6 | 7 | – | 1 | 3 | 6 | 39 | 57 | 39 | – | – | 20 | 29 | 36 | 34 | 30 | 55 | 44 |
Cytoplasm | 188 | 162 | 117 | 143 | 189 | 209 | 170 | 234 | – | 236 | 116 | 168 | 544 | 553 | 679 | – | – | 128 | 101 | 78 | 35 | 55 | 145 | 104 |
Endoplasmic Reticulum | 5 | 6 | 1 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 6 | 5 | 3 | 5 | – | – | 15 | 4 | 3 | 4 | 2 | 3 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | – | 0 | 0 | 2 | 0 | 0 | 1 | – | – | 1 | 4 | 3 | 2 | 1 | 2 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | – | – | 0 | 0 | 0 | 2 | 0 | 0 | 1 |
Mitochondria | 9 | 17 | 9 | 18 | 13 | 54 | 81 | 94 | – | 164 | 121 | 197 | 0 | 0 | 0 | – | – | 15 | 26 | 20 | 19 | 28 | 38 | 31 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | – | 2 | 1 | 1 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 7 | 6 | 7 | 8 | 2 | 4 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 5 | 2 | 1 | 2 | 1 | 2 | 2 |
SpindlePole | 7 | 1 | 3 | 7 | 9 | 3 | 7 | 11 | – | 10 | 9 | 9 | 24 | 9 | 8 | – | – | 27 | 1 | 1 | 0 | 1 | 3 | 4 |
Vac/Vac Membrane | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | – | – | 3 | 6 | 5 | 3 | 2 | 7 | 6 |
Unique Cell Count | 239 | 220 | 151 | 187 | 230 | 258 | 234 | 309 | 348 | 205 | 324 | 604 | 596 | 719 | 215 | 269 | 255 | 164 | 199 | 385 | 302 | |||
Labelled Cell Count | 278 | 251 | 173 | 225 | 257 | 316 | 291 | 368 | 441 | 269 | 415 | 682 | 678 | 777 | 279 | 269 | 255 | 164 | 199 | 385 | 302 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 4.1 | 4.0 | 4.0 | 3.9 | 3.1 | 3.2 | 3.0 | – | 2.4 | 2.7 | 2.6 | 5.2 | 5.1 | 5.5 | 4.0 | 4.3 | 5.0 | 5.2 | 5.4 | 5.6 |
Std Deviation (1e-4) | 0.7 | 1.0 | 1.6 | 1.6 | 1.6 | 1.1 | 1.7 | 1.3 | – | 0.9 | 0.8 | 0.6 | 0.8 | 0.6 | 0.9 | 1.0 | 0.8 | 0.9 | 1.1 | 1.2 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.03 | -0.35 | -0.32 | -0.4 | – | -0.71 | -0.57 | -0.64 | 0.39 | 0.34 | 0.46 | 0.01 | 0.1 | 0.34 | 0.38 | 0.44 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.9288 | 1.1737 | 1.1623 | 2.0256 | 1.3021 | 1.5074 | 0.8802 | 1.3994 | 1.5278 | 2.4246 | 2.0179 | 1.8752 | 0.8591 | 0.8944 | 0.8805 | 1.3951 | 0.903 | 1.0838 |
Actin | 0.0597 | 0.0235 | 0.0451 | 0.0237 | 0.0442 | 0.037 | 0.0475 | 0.0158 | 0.055 | 0.0095 | 0.0086 | 0.0658 | 0.0052 | 0.0075 | 0.0267 | 0.0046 | 0.0097 | 0.0322 |
Bud | 0.0056 | 0.0092 | 0.0069 | 0.0191 | 0.0157 | 0.0062 | 0.0027 | 0.0018 | 0.005 | 0.0006 | 0.0032 | 0.0017 | 0.0014 | 0.0023 | 0.003 | 0.0021 | 0.0013 | 0.0017 |
Bud Neck | 0.0382 | 0.0097 | 0.0645 | 0.0973 | 0.0631 | 0.1635 | 0.0357 | 0.0093 | 0.0545 | 0.1164 | 0.1685 | 0.1829 | 0.028 | 0.0098 | 0.0575 | 0.1588 | 0.2288 | 0.1689 |
Bud Periphery | 0.0272 | 0.0232 | 0.0589 | 0.0521 | 0.0397 | 0.0401 | 0.0171 | 0.0069 | 0.0343 | 0.0073 | 0.031 | 0.0192 | 0.0056 | 0.0138 | 0.0147 | 0.0666 | 0.011 | 0.0275 |
Bud Site | 0.0538 | 0.248 | 0.1279 | 0.0592 | 0.02 | 0.0176 | 0.1096 | 0.324 | 0.1077 | 0.0047 | 0.0425 | 0.0145 | 0.0361 | 0.2701 | 0.1171 | 0.0257 | 0.019 | 0.0039 |
Cell Periphery | 0.1109 | 0.0724 | 0.1075 | 0.0925 | 0.106 | 0.099 | 0.2311 | 0.1094 | 0.1633 | 0.2088 | 0.2968 | 0.199 | 0.3073 | 0.0623 | 0.1378 | 0.1439 | 0.0967 | 0.1683 |
Cytoplasm | 0.1766 | 0.2036 | 0.1965 | 0.2626 | 0.1807 | 0.2298 | 0.3468 | 0.2316 | 0.2252 | 0.3082 | 0.2507 | 0.274 | 0.2196 | 0.2268 | 0.2881 | 0.214 | 0.1887 | 0.3589 |
Cytoplasmic Foci | 0.0163 | 0.0084 | 0.015 | 0.0218 | 0.0379 | 0.0183 | 0.023 | 0.0032 | 0.0176 | 0.0094 | 0.0103 | 0.0047 | 0.0043 | 0.0038 | 0.0051 | 0.0049 | 0.005 | 0.0161 |
Eisosomes | 0.0099 | 0.0093 | 0.0042 | 0.0021 | 0.0026 | 0.0021 | 0.0083 | 0.0028 | 0.0059 | 0.0037 | 0.0015 | 0.0032 | 0.0088 | 0.0027 | 0.005 | 0.0026 | 0.0005 | 0.001 |
Endoplasmic Reticulum | 0.0104 | 0.0024 | 0.0023 | 0.0053 | 0.0065 | 0.0026 | 0.0016 | 0.0009 | 0.0028 | 0.0038 | 0.0036 | 0.001 | 0.0023 | 0.0025 | 0.0022 | 0.0022 | 0.0008 | 0.0013 |
Endosome | 0.0209 | 0.0007 | 0.0074 | 0.016 | 0.0172 | 0.0029 | 0.0018 | 0.0002 | 0.0039 | 0.0119 | 0.0011 | 0.0003 | 0.0001 | 0.0005 | 0.0003 | 0.0003 | 0.0001 | 0.0003 |
Golgi | 0.0068 | 0.0012 | 0.0044 | 0.0052 | 0.0069 | 0.0013 | 0.0026 | 0.0004 | 0.0064 | 0.0234 | 0.0003 | 0.0006 | 0.0004 | 0.0004 | 0.0008 | 0.0003 | 0.0003 | 0.0005 |
Lipid Particles | 0.0135 | 0.0008 | 0.008 | 0.0155 | 0.0257 | 0.0019 | 0.0105 | 0.0003 | 0.0049 | 0.0149 | 0.0002 | 0.0005 | 0.001 | 0.0007 | 0.0007 | 0.0012 | 0.0004 | 0.0015 |
Mitochondria | 0.006 | 0.0014 | 0.0065 | 0.0068 | 0.0028 | 0.0011 | 0.0009 | 0.0002 | 0.0028 | 0.029 | 0.0028 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0002 |
None | 0.3948 | 0.3713 | 0.3235 | 0.2417 | 0.3859 | 0.3548 | 0.1495 | 0.2915 | 0.3017 | 0.2369 | 0.131 | 0.2289 | 0.3765 | 0.3931 | 0.3368 | 0.3689 | 0.4361 | 0.2151 |
Nuclear Periphery | 0.014 | 0.0005 | 0.0042 | 0.0121 | 0.0039 | 0.0042 | 0.0003 | 0.0001 | 0.0004 | 0.0003 | 0.0222 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0001 |
Nucleolus | 0.0018 | 0.001 | 0.0023 | 0.003 | 0.0023 | 0.0011 | 0.0008 | 0.0003 | 0.0005 | 0.0005 | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0003 | 0.0003 | 0.0001 | 0.0001 |
Nucleus | 0.0102 | 0.0022 | 0.0029 | 0.0185 | 0.0088 | 0.0051 | 0.0005 | 0.0004 | 0.0007 | 0.0006 | 0.0216 | 0.0004 | 0.0004 | 0.0007 | 0.0006 | 0.0004 | 0.0001 | 0.0003 |
Peroxisomes | 0.007 | 0.0053 | 0.0061 | 0.0144 | 0.0189 | 0.0023 | 0.0064 | 0.0002 | 0.0042 | 0.0055 | 0.0002 | 0.0006 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0004 |
Punctate Nuclear | 0.0062 | 0.0038 | 0.0014 | 0.0192 | 0.0074 | 0.0042 | 0.0011 | 0.0004 | 0.0006 | 0.0002 | 0.0005 | 0.0001 | 0.0004 | 0.0003 | 0.0008 | 0.0003 | 0.0002 | 0.0001 |
Vacuole | 0.0078 | 0.002 | 0.0038 | 0.0093 | 0.0033 | 0.0047 | 0.0023 | 0.0007 | 0.0024 | 0.0038 | 0.0022 | 0.0018 | 0.0014 | 0.0014 | 0.0017 | 0.0021 | 0.0006 | 0.0015 |
Vacuole Periphery | 0.0025 | 0.0001 | 0.0007 | 0.0024 | 0.0006 | 0.0003 | 0.0001 | 0 | 0.0002 | 0.0006 | 0.001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.5462 | 8.2641 | 9.1984 | 7.5935 | 6.4211 | 11.2193 | 12.5337 | 10.8764 | 8.6212 | 10.7425 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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