Standard name | |
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Human Ortholog | |
Description | Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of TRAMP; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; required for mRNA surveillance and maintenance of genome integrity, serving as a link between RNA and DNA metabolism; overlapping but non-redundant functions with Trf5p; relocalizes to cytosol in response to hypoxia |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | – | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 1 | 0 | 2 | 4 | 2 | 7 | 13 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 6 | – | 9 | 12 | 6 | 22 | 21 | 9 | 17 | 12 | 9 | 3 | 20 | 46 | 10 | 6 | 13 | 1 | 0 | 0 | 1 | 1 | 4 |
Endoplasmic Reticulum | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 3 | 0 | 1 | 0 | 0 | 1 | 1 |
Endosome | 0 | 0 | – | 0 | 2 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 |
Golgi | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 0 |
Mitochondria | 21 | 1 | – | 1 | 0 | 11 | 35 | 31 | 50 | 49 | 75 | 64 | 0 | 0 | 1 | 8 | 4 | 13 | 2 | 0 | 0 | 0 | 1 | 3 |
Nucleus | 159 | 240 | – | 203 | 284 | 254 | 431 | 562 | 223 | 365 | 235 | 300 | 49 | 154 | 165 | 268 | 204 | 287 | 154 | 235 | 32 | 27 | 115 | 120 |
Nuclear Periphery | 0 | 0 | – | 1 | 0 | 1 | 7 | 4 | 1 | 4 | 1 | 5 | 0 | 2 | 4 | 1 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 4 | 1 | – | 0 | 3 | 2 | 9 | 13 | 23 | 15 | 13 | 31 | 1 | 14 | 12 | 16 | 11 | 18 | 1 | 2 | 0 | 0 | 5 | 7 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | 0 | 0 | 0 | 2 | 5 | 1 | 7 | 0 | 4 | 0 | 0 | 5 | 0 | 0 | 3 | 0 | 0 | 0 | 1 | 1 | 0 |
Vac/Vac Membrane | 0 | 5 | – | 1 | 9 | 4 | 7 | 17 | 1 | 9 | 4 | 6 | 1 | 2 | 8 | 12 | 7 | 15 | 4 | 5 | 1 | 2 | 15 | 41 |
Unique Cell Count | 169 | 251 | 213 | 306 | 265 | 477 | 617 | 250 | 414 | 274 | 366 | 52 | 180 | 231 | 302 | 227 | 327 | 171 | 253 | 39 | 37 | 149 | 185 | |
Labelled Cell Count | 190 | 254 | 215 | 311 | 279 | 515 | 659 | 313 | 475 | 348 | 434 | 54 | 193 | 248 | 321 | 240 | 357 | 171 | 253 | 39 | 37 | 149 | 185 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.7 | 8.0 | – | 7.6 | 7.8 | 6.5 | 6.2 | 6.5 | 5.2 | 5.5 | 5.3 | 5.4 | 8.3 | 8.0 | 7.9 | 10.8 | 11.0 | 9.7 | – | 7.5 | 8.1 |
Std Deviation (1e-4) | 0.8 | 1.6 | – | 1.6 | 1.6 | 2.6 | 1.6 | 1.5 | 1.1 | 1.3 | 1.4 | 1.6 | 1.5 | 1.6 | 1.8 | 2.7 | 2.8 | 2.6 | – | 1.4 | 1.2 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9512 | 4.3079 | 3.802 | 3.4633 | 3.4399 | 3.771 | 3.5708 | 4.0713 | 3.8116 | 3.4349 | 3.3925 | 3.7495 | 1.669 | 2.5473 | 1.8128 | 1.8388 | 1.4578 | 1.5067 |
Actin | 0.0134 | 0.0001 | 0.0123 | 0.0006 | 0.0423 | 0.0027 | 0.0418 | 0 | 0.0246 | 0.0017 | 0.079 | 0.017 | 0.0139 | 0.0099 | 0.0069 | 0.0097 | 0.0006 | 0 |
Bud | 0.0008 | 0.0001 | 0.0004 | 0.0006 | 0.0007 | 0.0002 | 0.0007 | 0 | 0.0005 | 0.0002 | 0.0027 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 |
Bud Neck | 0.0029 | 0.0003 | 0.0052 | 0.0011 | 0.0011 | 0.001 | 0.002 | 0.0001 | 0.0014 | 0.0007 | 0.0098 | 0.0026 | 0.0013 | 0.0001 | 0.0002 | 0.0003 | 0.0008 | 0.0008 |
Bud Periphery | 0.0023 | 0.0003 | 0.0006 | 0.0023 | 0.0008 | 0.0004 | 0.0012 | 0 | 0.001 | 0.0002 | 0.0038 | 0.0005 | 0.0012 | 0.0001 | 0.0003 | 0.0003 | 0.0009 | 0.0002 |
Bud Site | 0.0029 | 0.0006 | 0.0025 | 0.0007 | 0.0083 | 0.0001 | 0.0102 | 0.0001 | 0.0063 | 0.004 | 0.0215 | 0.0003 | 0.0019 | 0.0016 | 0.0004 | 0.0004 | 0.001 | 0.0001 |
Cell Periphery | 0.0013 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.001 | 0.0001 | 0.0005 | 0.0004 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.0026 | 0.0001 | 0.0033 | 0.0003 | 0.0006 | 0.0002 | 0.002 | 0.0001 | 0.0027 | 0.0001 | 0.0038 | 0.0041 | 0.0028 | 0 | 0.0013 | 0.0003 | 0.0005 | 0 |
Cytoplasmic Foci | 0.0092 | 0.0002 | 0.0082 | 0.0003 | 0.0154 | 0.0001 | 0.0079 | 0 | 0.0045 | 0.0103 | 0.0303 | 0.0029 | 0.0121 | 0 | 0.0014 | 0.0142 | 0.0021 | 0 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0005 | 0 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0039 | 0.0001 | 0.0032 | 0.0007 | 0.0008 | 0.0009 | 0.0022 | 0 | 0.0023 | 0 | 0.0033 | 0.0009 | 0.0016 | 0 | 0.001 | 0.0004 | 0.0006 | 0 |
Endosome | 0.0282 | 0.0005 | 0.0181 | 0.0023 | 0.0148 | 0.0013 | 0.0134 | 0 | 0.0033 | 0.0019 | 0.0166 | 0.0046 | 0.0066 | 0 | 0.0031 | 0.0282 | 0.0052 | 0 |
Golgi | 0.0084 | 0.0001 | 0.0086 | 0.0004 | 0.0178 | 0.0007 | 0.0059 | 0 | 0.0036 | 0.004 | 0.0153 | 0.0027 | 0.0029 | 0 | 0.0005 | 0.0206 | 0.0009 | 0 |
Lipid Particles | 0.0154 | 0.0005 | 0.0089 | 0.006 | 0.0154 | 0.0001 | 0.0184 | 0 | 0.0044 | 0.021 | 0.0103 | 0.0019 | 0.0102 | 0 | 0.0046 | 0.018 | 0.0042 | 0 |
Mitochondria | 0.0152 | 0.0039 | 0.0052 | 0.018 | 0.0089 | 0.0029 | 0.0127 | 0.0002 | 0.0061 | 0.0012 | 0.006 | 0.007 | 0.006 | 0.0004 | 0.0013 | 0.007 | 0.0045 | 0.0002 |
None | 0.0023 | 0.0001 | 0.0017 | 0.0004 | 0.0005 | 0.0001 | 0.0037 | 0 | 0.0031 | 0.0002 | 0.0065 | 0.0005 | 0.0149 | 0 | 0.0008 | 0.0012 | 0.0005 | 0 |
Nuclear Periphery | 0.0221 | 0.0032 | 0.0223 | 0.0103 | 0.0055 | 0.0053 | 0.0072 | 0.0039 | 0.0064 | 0.0007 | 0.0091 | 0.0034 | 0.0048 | 0.0005 | 0.0097 | 0.0025 | 0.0107 | 0.0042 |
Nucleolus | 0.0894 | 0.0681 | 0.0893 | 0.1508 | 0.0757 | 0.1046 | 0.0743 | 0.0869 | 0.0869 | 0.124 | 0.0546 | 0.1382 | 0.0722 | 0.0701 | 0.1244 | 0.1467 | 0.0975 | 0.1138 |
Nucleus | 0.7568 | 0.9117 | 0.7856 | 0.7832 | 0.7787 | 0.8757 | 0.7781 | 0.9067 | 0.8287 | 0.7708 | 0.6664 | 0.808 | 0.8322 | 0.9153 | 0.8339 | 0.7253 | 0.8453 | 0.8796 |
Peroxisomes | 0.0029 | 0.0001 | 0.0041 | 0.0003 | 0.0077 | 0 | 0.0088 | 0 | 0.0046 | 0.0443 | 0.0443 | 0.0013 | 0.0071 | 0 | 0.0002 | 0.0157 | 0.0004 | 0 |
Punctate Nuclear | 0.0063 | 0.0081 | 0.0161 | 0.0091 | 0.0029 | 0.0027 | 0.0033 | 0.0014 | 0.0026 | 0.014 | 0.0128 | 0.0008 | 0.0035 | 0.001 | 0.0063 | 0.0073 | 0.0189 | 0.0003 |
Vacuole | 0.0086 | 0.0011 | 0.0026 | 0.0063 | 0.0011 | 0.0004 | 0.0026 | 0.0002 | 0.0044 | 0.0004 | 0.002 | 0.0021 | 0.0022 | 0.0004 | 0.0021 | 0.0008 | 0.0031 | 0.0003 |
Vacuole Periphery | 0.0049 | 0.0008 | 0.0016 | 0.0056 | 0.0007 | 0.0004 | 0.0024 | 0.0001 | 0.0014 | 0.0001 | 0.0011 | 0.0008 | 0.0012 | 0.0003 | 0.0012 | 0.0008 | 0.0019 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.0621 | 22.4815 | 21.6869 | 19.3179 | 26.4928 | 26.3916 | 20.7421 | 26.273 | 23.1241 | 23.7658 |
Translational Efficiency | 0.9802 | 0.8662 | 0.8168 | 0.7052 | 0.615 | 0.6466 | 0.861 | 0.6247 | 0.6107 | 0.6904 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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