Standard name
Human Ortholog
Description Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0 0.05 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.06 0.06 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.32 0.33 0.29 0.25 0.2 0.27 0.13 0.18 0.12 0.09 0.12 0.12 0.31 0.41 0.42 0.16 0.18 0.13 0.17 0.19 0.08 0.26 0.2 0.19
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.08 0.06 0.28 0.14 0.23 0.14 0.07 0.09 0 0 0 0 0.16 0.15 0.14 0.11 0.14 0.21 0.12 0.11 0.23 0.09 0.09 0.12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.51 0.25 0.2 0.42 0.29 0.44 0.66 0.51 0.66 0.79 0.76 0.81 0 0 0 0.17 0.1 0.11 0.11 0.08 0.15 0.07 0.07 0.06
Nucleus 0.07 0.33 0.1 0.14 0.16 0.11 0.13 0.17 0.15 0.16 0.15 0.12 0.21 0.3 0.33 0.5 0.47 0.39 0.07 0.14 0.06 0.08 0.07 0.08
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0.05 0.11 0.12 0.09 0.11 0.09 0.12 0.1 0.11 0.09 0.06 0 0 0.11 0.15 0.17 0 0.08 0.06 0.1 0.06 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.12 0.06 0.22 0.17 0.19 0.19 0.15 0.21 0.19 0.15 0.12 0.09 0.33 0.17 0.14 0.06 0.09 0.09 0.28 0.22 0.25 0.27 0.35 0.33
Vac/Vac Membrane 0.13 0.12 0.06 0 0.05 0.05 0 0 0 0 0 0 0 0.05 0.07 0.13 0.11 0.21 0.1 0.08 0 0.05 0.06 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3
Bud 0 0 2 6 10 3 22 11 18 22 23 18 1 0 1 0 1 0 2 1 1 1 6 10
Bud Neck 0 0 2 2 3 1 7 3 2 5 1 3 8 1 3 0 0 0 0 2 0 0 1 4
Bud Site 0 0 1 0 5 5 15 11 9 21 21 29 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 54 16 55 66 65 74 56 58 40 32 44 53 106 122 169 14 19 11 36 22 18 47 81 83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 2 2 1 0 2 0 2 1
Endosome 13 3 53 38 77 39 32 28 11 2 4 3 55 45 55 10 15 18 26 12 54 17 36 50
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 3 0 0 4
Mitochondria 86 12 38 112 96 120 291 165 223 293 284 351 8 5 2 15 10 10 22 9 36 12 29 25
Nucleus 11 16 19 38 52 30 56 55 51 60 58 54 71 91 132 45 49 34 14 15 14 14 29 32
Nuclear Periphery 2 0 1 5 2 2 5 2 5 2 4 4 0 1 0 3 4 0 4 2 8 2 1 4
Nucleolus 9 2 10 28 40 25 47 29 41 36 43 37 19 12 6 10 16 15 7 9 13 17 22 21
Peroxisomes 0 0 5 3 9 3 5 11 1 3 3 0 4 1 0 0 0 0 0 0 2 0 0 1
SpindlePole 20 3 42 45 63 53 67 67 63 56 46 41 113 51 56 5 9 8 60 25 60 50 142 142
Vac/Vac Membrane 22 6 11 5 17 13 16 6 5 5 6 17 10 15 28 12 11 18 20 9 9 9 26 28
Unique Cell Count 167 48 190 264 333 273 439 322 337 373 376 433 340 300 405 90 104 87 213 116 240 183 409 433
Labelled Cell Count 217 58 239 349 440 368 619 447 469 537 537 610 395 346 454 116 136 116 213 116 240 183 409 433


Microtubule

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.2 4.5 4.0 4.2 4.9 3.9 3.6 3.4 3.2 3.1 3.0 5.3 5.3 5.2 7.1 7.2 6.6 6.0 5.4 6.2
Std Deviation (1e-4) 0.4 0.7 1.9 1.6 2.1 5.0 2.7 1.5 1.7 1.3 1.8 1.0 1.1 1.1 1.0 1.8 2.1 2.1 2.6 1.6 2.4
Intensity Change (Log2) – – – -0.18 -0.09 0.13 -0.21 -0.34 -0.4 -0.49 -0.52 -0.61 0.24 0.22 0.21 0.65 0.67 0.55 0.41 0.26 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.1 1.5 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3751 0.3062 0.3206 0.6146 0.6065 0.5026 -0.8974 -0.575 -0.5276 -0.1643 -0.2015 -0.7787 -1.9476 -1.6233 -1.7548 -1.6396 -2.0474 -1.8265
Actin 0.0318 0.0006 0.0089 0.011 0.0493 0.0008 0.0192 0.0003 0.0211 0.0522 0.0591 0.0052 0.0332 0.0006 0.0036 0.0265 0.004 0.0004
Bud 0.0007 0.0007 0.0015 0.0032 0.0201 0.0018 0.0005 0.0001 0.0015 0.0004 0.0019 0.0002 0.0005 0.0002 0.0005 0.0002 0.0001 0.0001
Bud Neck 0.007 0.0007 0.001 0.0006 0.0007 0.0026 0.001 0.001 0.0026 0.0009 0.0026 0.0037 0.0024 0.0007 0.0021 0.0006 0.0011 0.0121
Bud Periphery 0.0012 0.0004 0.0008 0.0175 0.0069 0.0008 0.0009 0.0001 0.0027 0.0006 0.0034 0.0003 0.0005 0.0004 0.0011 0.0003 0.0001 0.0001
Bud Site 0.0138 0.0047 0.0024 0.0003 0.0011 0.0003 0.0046 0.0016 0.0054 0.0012 0.0039 0.0005 0.0031 0.0022 0.0012 0.0005 0.0003 0.0003
Cell Periphery 0.0002 0.0001 0.0001 0.0002 0.0004 0.0001 0.0003 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0 0 0
Cytoplasm 0.0476 0.0404 0.0398 0.0374 0.0342 0.0652 0.0938 0.1156 0.1378 0.0963 0.046 0.1496 0.0434 0.0576 0.0619 0.0359 0.0333 0.0818
Cytoplasmic Foci 0.0314 0.0345 0.0421 0.0226 0.1094 0.0264 0.0459 0.0758 0.0402 0.0253 0.0903 0.0737 0.0962 0.0661 0.0704 0.0632 0.1125 0.0538
Eisosomes 0.0003 0.0001 0.0002 0.0003 0.0005 0.0001 0.0003 0.0001 0.0003 0.0002 0.0003 0.0001 0.0002 0.0001 0.0002 0.0002 0.0001 0
Endoplasmic Reticulum 0.003 0.0017 0.0022 0.0032 0.0037 0.001 0.0051 0.0044 0.0054 0.0052 0.0044 0.0042 0.0028 0.0024 0.0024 0.0016 0.0019 0.0027
Endosome 0.016 0.0084 0.0103 0.0103 0.0337 0.0054 0.0294 0.0201 0.0316 0.0136 0.0708 0.0226 0.022 0.0117 0.0218 0.0134 0.0193 0.0102
Golgi 0.0039 0.0007 0.0018 0.0009 0.0138 0.0014 0.0052 0.0009 0.0077 0.007 0.0283 0.0039 0.0089 0.001 0.0033 0.0071 0.0034 0.0011
Lipid Particles 0.0189 0.022 0.0144 0.0042 0.0329 0.0097 0.0353 0.0168 0.0192 0.0121 0.0202 0.0179 0.0386 0.0109 0.031 0.0506 0.0346 0.0302
Mitochondria 0.0017 0.0012 0.0018 0.0033 0.02 0.0032 0.008 0.0004 0.0227 0.0425 0.0155 0.0027 0.0024 0.0007 0.0043 0.0057 0.0006 0.0003
None 0.4451 0.5382 0.4682 0.4293 0.2071 0.5064 0.3144 0.3655 0.3183 0.2902 0.1693 0.2617 0.3244 0.4115 0.363 0.3321 0.2326 0.2793
Nuclear Periphery 0.0256 0.0216 0.0221 0.0695 0.0183 0.0108 0.0389 0.0282 0.0256 0.0477 0.0312 0.02 0.0206 0.0121 0.0321 0.0127 0.0134 0.0164
Nucleolus 0.0153 0.0133 0.0202 0.0026 0.0088 0.0152 0.01 0.0125 0.0142 0.0076 0.0072 0.029 0.0105 0.0066 0.0086 0.0448 0.0472 0.024
Nucleus 0.1122 0.0981 0.0885 0.017 0.0727 0.098 0.0839 0.1166 0.082 0.1241 0.0811 0.1129 0.0707 0.0534 0.0436 0.0425 0.038 0.1213
Peroxisomes 0.0053 0.0018 0.0064 0.002 0.0175 0.0014 0.009 0.002 0.011 0.0107 0.0151 0.0041 0.0084 0.0045 0.003 0.0446 0.014 0.0015
Punctate Nuclear 0.2164 0.2092 0.2654 0.3603 0.3443 0.2473 0.2875 0.2335 0.2419 0.2573 0.3404 0.2857 0.3093 0.3561 0.3419 0.3161 0.4424 0.363
Vacuole 0.0017 0.0013 0.0012 0.0015 0.0028 0.0016 0.0048 0.0036 0.0056 0.0024 0.0059 0.0017 0.0012 0.001 0.0022 0.0005 0.0008 0.0011
Vacuole Periphery 0.0008 0.0005 0.0006 0.0028 0.0015 0.0005 0.002 0.0007 0.0025 0.0024 0.0031 0.0005 0.0006 0.0003 0.0014 0.0009 0.0004 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.9246 8.5846 8.2812 12.8551 8.7157 12.4772 16.0579 11.7167 12.2321 13.9891
Translational Efficiency 0.4182 0.807 0.7951 0.5318 0.7095 0.6265 0.5938 0.6589 0.6662 0.6101

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages nucleus (23%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Msn1

Msn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available