Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0.05 0.05 – 0 0.05 0.06 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.95 1.0 0.9 0.88 0.81 – 0.79 0.72 0.81 0.75 0.97 0.97 0.99 0.98 0.97 0.93 0.98 0.92 0.93 0.93 0.9 0.89
Endoplasmic Reticulum 0 0 0 0 0 0.08 0.14 – 0.11 0.15 0.06 0.08 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.09 0.06 0.12 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 5 – 7 7 6 12 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 2 0 1 1 1 1 7 4
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 6 4 0 7 9 – 7 8 8 19 5 7 4 0 3 0 0 0 2 0 1 0
Cytoplasm 195 210 123 102 84 122 145 – 134 115 104 119 225 306 342 177 138 98 185 188 121 182 294 233
Endoplasmic Reticulum 1 2 4 0 2 11 25 – 18 24 8 12 7 5 2 9 5 5 0 2 3 1 9 10
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 1 – 3 4 3 10 0 0 0 3 0 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 3 1 1 – 15 10 15 13 0 0 0 2 1 0 0 1 0 0 1 0
Nucleus 0 1 0 0 0 0 1 – 0 0 2 0 0 1 0 0 0 0 0 5 0 3 0 0
Nuclear Periphery 0 0 0 0 1 0 0 – 0 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 0 5 1 2 – 0 2 3 3 1 1 3 1 1 5 1 3 0 2 4 7
Unique Cell Count 196 213 129 102 93 138 179 170 159 128 158 233 315 347 180 142 105 190 205 131 195 326 262
Labelled Cell Count 198 216 133 106 95 143 189 184 171 150 189 238 320 351 192 150 109 190 205 131 195 326 262


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 59.1 52.8 55.1 50.5 27.8 31.6 28.3 – 24.8 24.2 23.2 22.4 52.2 51.5 47.4 65.2 84.8 76.0 56.2 60.1 55.9
Std Deviation (1e-4) 12.2 13.7 11.6 9.1 10.6 7.7 7.0 – 6.1 6.5 7.0 6.8 14.7 10.9 13.1 20.3 24.0 27.1 16.9 16.8 18.7
Intensity Change (Log2) – – – -0.13 -0.99 -0.8 -0.96 – -1.16 -1.19 -1.25 -1.3 -0.08 -0.1 -0.22 0.24 0.62 0.46 0.03 0.12 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 3.2 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.2 0.2 – -0.2 0.1 0.6 2.2 0 0 0 0 0 0
Cytoplasm 2.2 -1.5 -2.1 -3.7 – -4.1 -5.1 -3.5 -4.6 0.6 0.9 2.1 1.5 0.8 -0.7
Endoplasmic Reticulum 0 0 1.7 3.2 – 2.5 3.4 1.2 1.7 0 0 0 0.8 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 3.5 0 4.0 3.3 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 61.2611 76.9483 69.9087 71.3644 64.9003 70.4138 71.7325 100.6957 90.5123 91.8757 80.4538 89.7652 62.3694 80.1286 75.8274 62.846 78.3275 66.2988
Actin 0.0146 0.0003 0.0109 0.0001 0.051 0.0031 0.0054 0.0002 0.0057 0.0001 0.0009 0.0006 0.0153 0.0003 0.0183 0.0109 0.0008 0.0092
Bud 0.0013 0.0004 0.0005 0.0013 0.0007 0.0036 0.0019 0.0004 0.0017 0.0007 0.0031 0.0005 0.0021 0.0002 0.0005 0.003 0.0002 0.0034
Bud Neck 0.0014 0.0003 0.0014 0.0006 0.0035 0.0024 0.0008 0.0007 0.0011 0.0009 0.0011 0.0037 0.0008 0.0002 0.0005 0.0009 0.0005 0.0022
Bud Periphery 0.0016 0.0008 0.0004 0.0007 0.0005 0.0052 0.0032 0.0005 0.0022 0.0005 0.0041 0.0003 0.0044 0.0001 0.0004 0.0011 0.0001 0.0055
Bud Site 0.0036 0.0001 0.0009 0.0002 0.0002 0.0011 0.0011 0.0005 0.0006 0.0001 0.0012 0.0001 0.0052 0.0002 0.0008 0.0084 0 0.016
Cell Periphery 0.0076 0.0047 0.0033 0.0037 0.0023 0.0056 0.0119 0.0106 0.007 0.0117 0.0072 0.003 0.0014 0.0007 0.0025 0.0025 0.0028 0.0008
Cytoplasm 0.7531 0.9575 0.8506 0.7619 0.8076 0.8029 0.7608 0.9235 0.8652 0.8184 0.7999 0.8753 0.7907 0.977 0.9166 0.7707 0.9092 0.879
Cytoplasmic Foci 0.0051 0.001 0.0056 0.0052 0.0038 0.0049 0.0082 0.0005 0.0051 0.001 0.0136 0.0052 0.0522 0.0007 0.002 0.0175 0.0062 0.0123
Eisosomes 0.0006 0 0.0001 0 0.0002 0.0002 0.0005 0 0.0002 0 0.0004 0 0.0001 0 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.049 0.0174 0.0428 0.0196 0.0054 0.0709 0.0437 0.0177 0.0214 0.0062 0.0113 0.0462 0.0368 0.0123 0.0196 0.0043 0.0046 0.0164
Endosome 0.0097 0.002 0.0077 0.0345 0.0114 0.0208 0.0088 0.0012 0.0036 0.0039 0.0057 0.0043 0.0115 0.0008 0.0034 0.059 0.0376 0.0087
Golgi 0.0022 0.0004 0.0012 0.0011 0.0019 0.0028 0.0012 0.0001 0.0006 0.0002 0.0006 0.0005 0.0047 0.0001 0.0008 0.0228 0.0012 0.0027
Lipid Particles 0.0033 0.0004 0.0016 0.0022 0.0008 0.0023 0.0032 0.0008 0.0025 0.001 0.0051 0.001 0.0035 0.0001 0.0007 0.0132 0.0006 0.0029
Mitochondria 0.0084 0.0004 0.0015 0.0007 0.0016 0.004 0.0073 0.0011 0.0059 0.0072 0.0181 0.0006 0.0038 0.0001 0.0008 0.0065 0.0003 0.0049
None 0.0042 0.0001 0.005 0.0011 0.0014 0.0025 0.0074 0.0002 0.0049 0.0009 0.0146 0.0037 0.005 0.0001 0.0079 0.0011 0.0002 0.0112
Nuclear Periphery 0.0302 0.0019 0.0075 0.0167 0.0178 0.0068 0.0242 0.0028 0.0075 0.011 0.0099 0.0097 0.017 0.001 0.0046 0.0081 0.0038 0.0062
Nucleolus 0.0036 0 0.004 0.0002 0.0017 0.0022 0.0093 0.0004 0.0026 0.0035 0.0097 0.0005 0.0004 0 0.0002 0.0003 0 0.0016
Nucleus 0.0109 0.001 0.005 0.0034 0.0065 0.0044 0.0119 0.0028 0.0077 0.0061 0.013 0.0074 0.0083 0.0008 0.0018 0.0038 0.0013 0.0048
Peroxisomes 0.0107 0 0.0059 0.0001 0.0001 0.001 0.0004 0 0.0003 0.0001 0.0009 0.0001 0.0025 0 0.0004 0.0051 0 0.0006
Punctate Nuclear 0.0012 0 0.0012 0.0004 0.0015 0.0024 0.0015 0 0.0009 0 0.0043 0.0003 0.0077 0 0.0003 0.0015 0.0001 0.0035
Vacuole 0.0586 0.0098 0.0366 0.1162 0.0559 0.0341 0.0727 0.0323 0.0448 0.0995 0.0606 0.0323 0.0182 0.0048 0.0147 0.0453 0.0271 0.0055
Vacuole Periphery 0.0191 0.0013 0.0064 0.0302 0.0241 0.0168 0.0147 0.0037 0.0085 0.027 0.0148 0.0047 0.0084 0.0004 0.0032 0.0136 0.0034 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1279.6955 1448.9526 1733.0843 1406.8647 1530.9194 1300.5359 1189.9106 1133.483 1307.3258 1180.3971
Translational Efficiency 1.5448 1.4956 1.3598 1.5058 1.6422 1.5684 1.6209 1.6764 1.539 1.8325

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rps19a

Rps19a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps19a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available