Standard name
Human Ortholog
Description Cytoplasmic malate dehydrogenase; one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0.05 0.09 0.07 0.09 0 0.09 0.1 0 0.07 0.08 0.09 0 0 0 0 0 0.06 0 0.05 0.06 0.09
Bud 0.08 0.07 0.11 0.12 0.15 0.18 0.18 0.17 0.17 0.19 0.17 0.15 0.09 0.08 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0.1 0.06 0.05 0 0.05 0.08 0.07 0 0.07 0.05 0.06 0.06 0 0 0 0 0 0.06
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.09 0.07 0.1 0.25 0.12 0.1 0.14 0.09 0.09 0.06 0.06 0.06 0.09 0.13 0.18 0.16 0.16 0.09 0.12 0.06 0.11 0.14 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.38 0.47 0.16 0.24 0.11 0.21 0.27 0.19 0.34 0.33 0.54 0.42 0.1 0.1 0.11 0.08 0.1 0.09 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.07 0 0 0.05 0.05 0.05 0 0.05 0.05 0.05 0.05 0 0 0 0.07 0.07 0.08 0 0 0 0 0 0
Peroxisomes 0.67 0.55 0.7 0.73 0.58 0.66 0.57 0.61 0.59 0.59 0.44 0.54 0.63 0.64 0.69 0.57 0.56 0.58 0.71 0.56 0.72 0.5 0.56 0.7
SpindlePole 0.34 0.18 0.51 0.38 0.29 0.3 0.29 0.33 0.28 0.22 0.17 0.2 0.37 0.37 0.38 0.38 0.41 0.4 0.12 0.13 0.13 0.19 0.13 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 12 12 15 40 35 65 28 45 58 22 37 19 26 12 5 5 5 4 15 7 13 21 39
Bud 22 17 39 36 64 89 124 110 89 110 84 81 22 22 13 10 8 8 2 7 7 7 9 7
Bud Neck 0 1 3 5 0 3 2 1 2 2 2 1 1 1 1 1 3 0 2 4 4 6 5 2
Bud Site 27 14 18 10 19 40 51 22 34 27 28 34 8 4 3 8 7 12
Cell Periphery 0 1 0 0 0 2 3 4 6 7 3 2 1 0 2 1 0 0 0 0 0 0 0 0
Cytoplasm 2 3 9 3 20 5 15 22 8 15 7 7 6 12 10 12 4 13 0 0 0 2 0 3
Endoplasmic Reticulum 0 0 0 0 4 1 0 0 0 0 0 2 3 0 0 4 1 1 0 0 1 1 1 1
Endosome 11 21 24 30 104 60 72 92 47 53 32 32 15 25 43 46 41 31 25 32 21 27 52 25
Golgi 0 0 0 0 10 3 0 2 2 0 0 1 3 8 4 10 8 1 4 7 5 6 6 10
Mitochondria 101 116 58 71 46 105 187 121 175 188 271 223 24 27 36 20 26 16 2 8 1 6 10 5
Nucleus 2 2 1 1 0 2 2 6 0 1 1 1 2 0 2 1 2 3 1 0 0 1 0 0
Nuclear Periphery 2 1 0 0 0 2 0 1 1 2 3 3 0 0 0 2 1 0 0 0 0 0 0 0
Nucleolus 10 18 4 10 20 27 32 22 23 26 23 29 10 10 10 19 18 15 1 0 0 2 0 0
Peroxisomes 180 135 249 213 244 333 395 397 298 332 224 292 154 175 225 146 142 109 200 156 259 128 209 293
SpindlePole 92 44 181 110 122 151 200 213 142 122 85 106 91 102 122 96 104 75 34 35 47 48 49 21
Vac/Vac Membrane 0 2 0 3 4 2 5 18 6 10 8 9 1 1 3 3 3 5 0 2 0 0 2 3
Unique Cell Count 267 245 358 292 422 507 693 650 509 567 505 537 245 274 324 255 253 188 281 277 358 257 375 421
Labelled Cell Count 450 387 598 507 697 860 1153 1059 878 953 793 860 360 413 486 384 373 294 281 277 358 257 375 421


Peroxisomes

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 7.1 6.0 6.7 9.5 8.1 7.3 7.7 7.6 7.9 7.1 7.5 9.1 9.7 10.3 10.5 10.0 9.3 8.6 8.3 8.5
Std Deviation (1e-4) 1.0 1.5 1.4 1.5 2.5 2.2 1.9 2.1 2.2 2.4 2.1 2.4 2.1 2.4 3.1 3.0 3.1 2.4 2.7 2.2 2.4
Intensity Change (Log2) 0.17 0.65 0.43 0.28 0.36 0.33 0.39 0.24 0.31 0.6 0.68 0.77 0.8 0.74 0.63 0.52 0.47 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.1 3.4 2.3 3.6 0.7 3.2 3.9 0.7 2.3 2.4 3.2 0.2 -1.0 -1.0 -0.4
Bud 0.6 1.8 2.7 3.0 2.6 2.7 3.4 2.4 1.8 -0.8 -1.2 -3.4 -3.1 -3.5 -2.6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site -1.0 -0.3 1.7 1.4 -1.3 1.0 -0.2 0.3 0.8 -1.0 -2.4 -3.1 -1.1 -1.4 0.7
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.4 1.6 -1.8 -0.4 0.8 -1.0 0.1 -1.2 -1.3 -0.1 1.3 0.5 1.5 -0.8 2.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 1.6 6.7 2.5 2.0 3.5 1.3 1.4 -0.2 -0.5 -0.3 1.1 2.9 4.3 3.8 3.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.6 -2.2 1.7 3.9 1.0 5.9 5.7 11.2 8.0 -2.3 -2.3 -1.9 -3.1 -2.1 -2.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.0 2.9 3.3 3.0 2.2 2.8 2.9 2.9 3.3 2.4 2.1 1.8 4.1 3.9 4.2
Peroxisomes 0.9 -3.4 -1.2 -4.0 -2.7 -3.3 -3.4 -7.3 -4.5 -1.7 -1.5 0 -3.1 -3.4 -2.7
SpindlePole -3.3 -6.2 -6.2 -6.9 -5.5 -6.8 -9.2 -10.6 -9.7 -3.3 -3.3 -3.4 -3.2 -2.3 -2.4
Vacuole 0 0 0 0 3.2 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.8109 7.9354 7.891 7.0558 8.3726 7.9376 3.4306 5.1644 4.307 3.4433 4.0376 4.4724 3.8392 5.4795 4.8737 5.3389 4.0066 4.6555
Actin 0.1042 0.0547 0.0957 0.017 0.0124 0.0034 0.0375 0.046 0.0616 0.0016 0.0296 0.0405 0.0212 0.032 0.0256 0.0019 0.0347 0.0353
Bud 0.0004 0.0024 0.0009 0.0001 0.0001 0.0001 0.0013 0.0029 0.0026 0.0001 0.0005 0.0004 0.0002 0.003 0.0012 0 0.0032 0.0003
Bud Neck 0.0023 0.008 0.0027 0.0006 0.0007 0.0003 0.0016 0.004 0.009 0.0001 0.0112 0.0215 0.0004 0.0035 0.0019 0.0048 0.0004 0.0006
Bud Periphery 0.0005 0.0029 0.0009 0.0001 0.0001 0.0003 0.0015 0.0019 0.0014 0.0001 0.0005 0.0005 0.0002 0.0026 0.0012 0.0001 0.0031 0.0004
Bud Site 0.0035 0.0376 0.0136 0.0005 0.0003 0.0003 0.0128 0.0458 0.0104 0.0003 0.0022 0.001 0.0035 0.0294 0.0045 0.0003 0.0013 0.0008
Cell Periphery 0.0004 0.0005 0.0001 0 0.0001 0 0.0003 0.0002 0.0003 0 0.0001 0.0001 0.0001 0.0002 0.0001 0 0.0002 0.0001
Cytoplasm 0.0077 0.0005 0.0001 0 0.0001 0.0001 0.0068 0.0003 0.0002 0.0002 0.0003 0.0002 0.0009 0.0006 0.0002 0 0.0009 0.0001
Cytoplasmic Foci 0.2274 0.1935 0.1883 0.2586 0.2947 0.2029 0.232 0.278 0.2405 0.2376 0.3253 0.2234 0.1996 0.1947 0.2079 0.1471 0.343 0.2633
Eisosomes 0.0006 0.0001 0.0002 0 0 0 0.0003 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001
Endoplasmic Reticulum 0.0067 0.0001 0 0 0 0 0.0068 0 0 0.0007 0.0001 0 0.0007 0 0 0 0.0012 0.0003
Endosome 0.0343 0.0123 0.0033 0.0024 0.0097 0.003 0.0565 0.0095 0.0118 0.0247 0.0177 0.0111 0.0188 0.0164 0.0048 0.0057 0.0133 0.0053
Golgi 0.0103 0.0201 0.0103 0.0091 0.0408 0.0077 0.0319 0.0154 0.0235 0.0218 0.0359 0.0182 0.0158 0.0216 0.0191 0.0145 0.0192 0.0133
Lipid Particles 0.0774 0.0584 0.097 0.0484 0.1742 0.1542 0.1245 0.0727 0.0617 0.1857 0.2673 0.134 0.1189 0.0636 0.058 0.2055 0.1922 0.22
Mitochondria 0.0033 0.015 0.0233 0.0091 0.0123 0.0104 0.0183 0.0092 0.0205 0.0044 0.0039 0.0049 0.0179 0.0224 0.0222 0.0122 0.0579 0.0231
None 0.0044 0.0002 0.0003 0.0001 0 0.0001 0.0025 0.0001 0.0001 0.0001 0.0001 0 0.0009 0.0001 0.0001 0 0.0008 0.0002
Nuclear Periphery 0.0205 0.0001 0 0 0 0.0007 0.01 0.0002 0 0.0015 0 0 0.001 0.0001 0 0 0.018 0.0013
Nucleolus 0.0007 0.0159 0.0004 0.0001 0.0005 0.0028 0.0011 0.0096 0.0016 0.0008 0.0005 0.0003 0.0006 0.0019 0.0015 0.0004 0.0021 0.0069
Nucleus 0.0017 0.0014 0.0001 0 0 0.0006 0.0014 0.0009 0.0003 0.0001 0.0001 0 0.0003 0.0002 0.0004 0 0.0043 0.0005
Peroxisomes 0.4423 0.5685 0.5501 0.6502 0.3491 0.6013 0.4426 0.4917 0.5395 0.5043 0.3018 0.5419 0.5777 0.5909 0.6478 0.6059 0.2923 0.4216
Punctate Nuclear 0.048 0.0048 0.0125 0.0035 0.1043 0.0109 0.004 0.0071 0.014 0.0142 0.0022 0.0016 0.02 0.0145 0.0026 0.001 0.0044 0.006
Vacuole 0.0023 0.0025 0.0001 0.0001 0.0004 0.0007 0.0035 0.0036 0.0007 0.0007 0.0005 0.0002 0.0008 0.0016 0.0003 0.0001 0.0034 0.0002
Vacuole Periphery 0.0011 0.0005 0.0001 0.0001 0.0003 0.0004 0.003 0.0008 0.0003 0.0009 0.0002 0.0001 0.0004 0.0008 0.0003 0.0002 0.0042 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.7041 34.7928 16.7815 23.253 10.7233 40.8442 53.2082 45.1125 42.1392 36.6253
Translational Efficiency 1.1947 1.1773 1.6531 0.9866 1.6034 1.5373 1.2612 1.5586 1.4156 1.2065

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Cytoplasmic malate dehydrogenase; one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1
Localization
Cell Percentages peroxisome (75%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mdh2

Mdh2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mdh2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available