Standard name | |
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Human Ortholog | |
Description | Protein of unknown function with 8 putative transmembrane segments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 18 | 5 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 13 | 13 | 6 | 3 | 7 | 6 | 4 | 22 | 22 | 11 | 25 | 34 |
Bud | 2 | 5 | 12 | 13 | 6 | 23 | 26 | 27 | – | 20 | 27 | 29 | 2 | 2 | 1 | 1 | 3 | 4 | 2 | 6 | 2 | 1 | 6 | 10 |
Bud Neck | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 5 | 7 | 0 | 3 | 0 | 0 | 1 | 8 | 0 | 2 | 5 | 5 |
Bud Site | 0 | 1 | 1 | 0 | 0 | 6 | 2 | 1 | – | 7 | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | – | 2 | 2 | 0 | 3 | 3 | 2 | 2 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 38 | 205 | 120 | 130 | 185 | 231 | 246 | 317 | – | 171 | 127 | 110 | 208 | 208 | 248 | 181 | 117 | 90 | 22 | 74 | 23 | 21 | 41 | 53 |
Endoplasmic Reticulum | 5 | 40 | 6 | 3 | 3 | 0 | 1 | 0 | – | 8 | 3 | 6 | 11 | 11 | 8 | 9 | 18 | 13 | 1 | 6 | 7 | 5 | 13 | 10 |
Endosome | 22 | 27 | 67 | 46 | 54 | 28 | 12 | 18 | – | 1 | 0 | 4 | 35 | 43 | 55 | 39 | 77 | 61 | 42 | 134 | 115 | 78 | 86 | 116 |
Golgi | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3 | 3 | 2 | 0 | 0 | 1 | 11 | 41 | 54 | 43 | 52 | 79 |
Mitochondria | 23 | 131 | 93 | 76 | 60 | 217 | 295 | 400 | – | 430 | 416 | 448 | 9 | 9 | 3 | 41 | 20 | 16 | 58 | 161 | 138 | 171 | 207 | 216 |
Nucleus | 4 | 0 | 0 | 2 | 3 | 0 | 3 | 2 | – | 1 | 0 | 1 | 0 | 0 | 2 | 6 | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | – | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 |
Nucleolus | 3 | 0 | 0 | 0 | 2 | 0 | 5 | 5 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 27 | 11 | 17 | 5 | 2 | 7 | – | 2 | 1 | 0 | 16 | 14 | 5 | 0 | 2 | 5 | 3 | 9 | 30 | 18 | 15 | 14 |
SpindlePole | 4 | 10 | 18 | 11 | 11 | 15 | 20 | 17 | – | 13 | 10 | 16 | 18 | 13 | 15 | 28 | 35 | 18 | 2 | 18 | 17 | 15 | 18 | 15 |
Vac/Vac Membrane | 6 | 10 | 12 | 10 | 14 | 8 | 15 | 16 | – | 13 | 16 | 11 | 16 | 13 | 23 | 11 | 19 | 28 | 18 | 43 | 8 | 14 | 29 | 30 |
Unique Cell Count | 91 | 397 | 303 | 257 | 297 | 417 | 484 | 675 | 526 | 481 | 520 | 310 | 316 | 351 | 280 | 256 | 200 | 173 | 541 | 427 | 389 | 515 | 599 | |
Labelled Cell Count | 110 | 449 | 364 | 304 | 357 | 533 | 630 | 814 | 673 | 609 | 630 | 339 | 339 | 370 | 324 | 302 | 249 | 173 | 541 | 427 | 389 | 515 | 599 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.5 | 4.8 | 4.4 | 4.3 | 4.3 | 3.3 | 3.2 | 3.4 | – | 2.9 | 3.0 | 3.1 | 6.0 | 6.0 | 6.1 | 5.0 | 5.8 | 6.4 | 6.0 | 6.1 | 6.2 |
Std Deviation (1e-4) | 0.7 | 0.8 | 1.0 | 1.2 | 1.8 | 1.1 | 1.1 | 0.8 | – | 0.8 | 0.6 | 0.6 | 1.1 | 1.1 | 1.0 | 1.0 | 1.2 | 1.6 | 1.1 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.01 | -0.42 | -0.46 | -0.34 | – | -0.6 | -0.52 | -0.49 | 0.47 | 0.47 | 0.49 | 0.2 | 0.41 | 0.56 | 0.46 | 0.48 | 0.51 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 1.8 | 4.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.21 | 1.2747 | 1.497 | 0.9947 | 1.3903 | 1.0403 | 2.3141 | 2.7368 | 2.459 | 2.3467 | 2.2197 | 2.6498 | 3.1015 | 2.4554 | 2.5851 | 2.5863 | 2.6325 | 2.4274 |
Actin | 0.0224 | 0.0012 | 0.027 | 0.0073 | 0.0025 | 0.001 | 0.0392 | 0.0115 | 0.0018 | 0.0045 | 0.0054 | 0.0062 | 0.0341 | 0.0036 | 0.0141 | 0.0009 | 0.0004 | 0.0024 |
Bud | 0.0007 | 0.0014 | 0.0008 | 0.0014 | 0.002 | 0.0004 | 0.0007 | 0.0011 | 0.0005 | 0.0001 | 0.0013 | 0.0004 | 0.0002 | 0.0002 | 0.0019 | 0.0004 | 0.0001 | 0.0002 |
Bud Neck | 0.0016 | 0.0006 | 0.0042 | 0.0016 | 0.0022 | 0.0018 | 0.0016 | 0.0008 | 0.0003 | 0.0013 | 0.0018 | 0.0025 | 0.001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0039 |
Bud Periphery | 0.0011 | 0.0029 | 0.0013 | 0.0027 | 0.0048 | 0.001 | 0.002 | 0.0008 | 0.0009 | 0.0001 | 0.0077 | 0.0012 | 0.0002 | 0.0003 | 0.0034 | 0.0006 | 0.0001 | 0.0002 |
Bud Site | 0.0037 | 0.007 | 0.0086 | 0.0035 | 0.0077 | 0.0004 | 0.0008 | 0.0172 | 0.0011 | 0.0011 | 0.0011 | 0.0003 | 0.0009 | 0.001 | 0.0017 | 0.0015 | 0.0001 | 0.0003 |
Cell Periphery | 0.0005 | 0.0008 | 0.0006 | 0.0018 | 0.0004 | 0.0002 | 0.0003 | 0.0005 | 0.0004 | 0.0001 | 0.0007 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasm | 0.0888 | 0.1448 | 0.1123 | 0.1116 | 0.0385 | 0.1247 | 0.0649 | 0.1468 | 0.1074 | 0.0191 | 0.0555 | 0.1667 | 0.1084 | 0.1939 | 0.1356 | 0.1252 | 0.0858 | 0.2246 |
Cytoplasmic Foci | 0.1012 | 0.0978 | 0.1211 | 0.1438 | 0.213 | 0.1209 | 0.1548 | 0.1733 | 0.1351 | 0.2189 | 0.1458 | 0.1837 | 0.1602 | 0.0837 | 0.1186 | 0.1422 | 0.1145 | 0.1256 |
Eisosomes | 0.0006 | 0.0003 | 0.0007 | 0.0006 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0006 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0169 | 0.0085 | 0.0055 | 0.0091 | 0.002 | 0.0022 | 0.0077 | 0.0061 | 0.0037 | 0.001 | 0.003 | 0.0127 | 0.0046 | 0.009 | 0.0045 | 0.0074 | 0.0079 | 0.0085 |
Endosome | 0.0854 | 0.1439 | 0.135 | 0.164 | 0.2756 | 0.138 | 0.1433 | 0.1867 | 0.1741 | 0.2444 | 0.2212 | 0.1378 | 0.1157 | 0.1098 | 0.1141 | 0.2697 | 0.1578 | 0.1615 |
Golgi | 0.0087 | 0.012 | 0.0111 | 0.0206 | 0.0409 | 0.0192 | 0.0218 | 0.0204 | 0.0429 | 0.0293 | 0.0204 | 0.0163 | 0.0259 | 0.0103 | 0.0316 | 0.0342 | 0.0045 | 0.0283 |
Lipid Particles | 0.0167 | 0.0207 | 0.0206 | 0.0231 | 0.0223 | 0.0209 | 0.0497 | 0.0183 | 0.0365 | 0.0239 | 0.053 | 0.0252 | 0.0412 | 0.0086 | 0.0263 | 0.0215 | 0.0174 | 0.0219 |
Mitochondria | 0.0092 | 0.0135 | 0.0178 | 0.1039 | 0.033 | 0.0392 | 0.0183 | 0.0022 | 0.0113 | 0.0028 | 0.0091 | 0.0067 | 0.0042 | 0.0062 | 0.0048 | 0.0173 | 0.002 | 0.009 |
None | 0.5477 | 0.4985 | 0.451 | 0.3578 | 0.2897 | 0.4921 | 0.4371 | 0.3837 | 0.4557 | 0.3301 | 0.4085 | 0.412 | 0.3961 | 0.5539 | 0.5184 | 0.36 | 0.5958 | 0.3916 |
Nuclear Periphery | 0.0199 | 0.0034 | 0.0022 | 0.0077 | 0.0019 | 0.0012 | 0.0123 | 0.0015 | 0.002 | 0.001 | 0.0113 | 0.0013 | 0.0132 | 0.0023 | 0.0011 | 0.0022 | 0.0017 | 0.0041 |
Nucleolus | 0.0014 | 0.0013 | 0.002 | 0.0018 | 0.0065 | 0.0004 | 0.0004 | 0.0007 | 0.0004 | 0.0008 | 0.0029 | 0.0002 | 0.0009 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0002 |
Nucleus | 0.0153 | 0.0027 | 0.0022 | 0.003 | 0.0031 | 0.0011 | 0.0054 | 0.0017 | 0.0012 | 0.0007 | 0.0036 | 0.0006 | 0.0578 | 0.0009 | 0.0006 | 0.0008 | 0.0005 | 0.0011 |
Peroxisomes | 0.0062 | 0.0084 | 0.0262 | 0.0167 | 0.0294 | 0.0219 | 0.0283 | 0.0107 | 0.0163 | 0.0773 | 0.0246 | 0.0169 | 0.0135 | 0.0068 | 0.0178 | 0.0028 | 0.0041 | 0.0058 |
Punctate Nuclear | 0.0186 | 0.0024 | 0.0124 | 0.0031 | 0.0046 | 0.0015 | 0.0026 | 0.0081 | 0.0018 | 0.0392 | 0.0127 | 0.0016 | 0.0155 | 0.0012 | 0.0012 | 0.0054 | 0.001 | 0.0034 |
Vacuole | 0.0285 | 0.0251 | 0.0337 | 0.0113 | 0.0162 | 0.0088 | 0.0056 | 0.0066 | 0.0045 | 0.0033 | 0.0073 | 0.0058 | 0.0048 | 0.0057 | 0.0028 | 0.0043 | 0.0044 | 0.0055 |
Vacuole Periphery | 0.005 | 0.0029 | 0.0036 | 0.0034 | 0.0037 | 0.0031 | 0.0025 | 0.0011 | 0.0016 | 0.0009 | 0.0029 | 0.0016 | 0.0009 | 0.0012 | 0.0007 | 0.0029 | 0.0016 | 0.0017 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.3398 | 7.9227 | 10.7781 | 15.3275 | 11.0121 | 6.6433 | 9.6795 | 11.9397 | 12.9352 | 13.7821 |
Translational Efficiency | 1.3433 | 1.4259 | 1.2726 | 0.8693 | 0.9761 | 1.9244 | 1.1771 | 1.0646 | 1.0892 | 0.9865 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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