Standard name
Human Ortholog
Description Subunit of SEACAT, a subcomplex of the SEA complex; Rtc1p, along with Mtc5p and Sea4p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; null suppresses cdc13-1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.28 0.5 0.69 0.76 0.75 0.74 0.79 0.81 0.74 – 0.67 0.61 0.6 0.69 0.79 0.61 0.63 0.63 0 0.08 0.09 0.06 0.05 0.08
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0.3 0.11 0.05 0 0.1 0 0 0 0 – 0 0 0.1 0.06 0 0.1 0.12 0.11 0.38 0.18 0.19 0.2 0.26 0.26
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0.13 0.31 0.13 0.35 0.39 0.33 0.52 – 0.56 0.59 0 0.06 0.19 0 0 0 0.08 0.08 0.13 0.05 0.1 0.07
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.08 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Vac/Vac Membrane 0.53 0.48 0.2 0.11 0.2 0.07 0.06 0 0.05 – 0.07 0.09 0.39 0.31 0.13 0.35 0.38 0.35 0.41 0.59 0.52 0.59 0.45 0.46
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 3 2 2 0 0 2
Bud 0 1 5 1 0 6 8 9 10 – 7 5 3 2 7 2 0 4 0 2 2 2 2 5
Bud Neck 1 0 1 0 0 0 0 2 0 – 0 2 0 1 0 0 0 0 1 2 0 2 1 1
Bud Site 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 1 1 1 – 0 0 0 0 2 2 1 0 0 0 0 0 0 0
Cytoplasm 113 224 277 215 255 264 339 468 232 – 111 144 144 209 153 280 194 302 10 38 45 14 10 27
Endoplasmic Reticulum 1 2 0 1 0 6 0 4 0 – 0 1 1 4 2 21 14 22 2 1 5 1 3 3
Endosome 120 48 20 0 34 5 6 6 3 – 0 0 25 19 1 48 36 52 180 93 93 48 53 88
Golgi 2 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 4 0 1 1 1 0
Mitochondria 4 30 51 88 44 127 169 194 163 – 93 139 9 19 36 19 6 20 36 41 65 12 19 22
Nucleus 3 8 1 4 0 2 3 4 2 – 1 4 0 1 3 3 2 7 1 3 1 2 1 1
Nuclear Periphery 3 7 0 0 0 2 0 0 1 – 3 0 0 1 3 1 0 0 13 3 4 1 5 4
Nucleolus 7 2 0 0 0 0 1 0 0 – 0 1 0 2 0 5 0 1 4 1 0 3 0 2
Peroxisomes 0 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 1 1 0 0 0 0 0 0
SpindlePole 32 9 6 2 3 3 3 5 0 – 0 2 0 1 2 7 8 3 12 9 3 6 9 15
Vac/Vac Membrane 212 216 80 31 69 25 26 20 15 – 11 21 95 95 26 160 118 166 194 299 256 144 93 158
Unique Cell Count 397 447 401 284 340 359 430 581 312 166 235 242 305 194 458 309 481 470 504 490 246 208 341
Labelled Cell Count 499 549 443 342 406 440 556 713 427 226 319 277 354 235 548 381 579 470 504 490 246 208 341


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 4.3 3.8 3.7 4.2 3.7 3.5 3.5 3.4 – 3.3 3.2 4.9 4.1 3.1 6.1 6.9 6.3 4.7 4.9 5.2
Std Deviation (1e-4) 0.8 0.7 0.8 1.2 1.7 1.3 1.1 1.4 1.6 – 1.3 1.3 2.4 1.2 1.0 1.4 2.0 1.8 1.4 1.8 1.7
Intensity Change (Log2) – – – -0.02 0.17 -0.03 -0.12 -0.09 -0.15 – -0.18 -0.21 0.39 0.14 -0.27 0.69 0.89 0.74 0.34 0.37 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.9 5.4 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9406 3.4232 3.1666 3.1676 3.9172 3.2181 -1.6175 -0.7393 -0.8805 -1.0852 -1.1594 -0.8308 3.5202 3.7092 3.0752 3.936 3.1506 3.4628
Actin 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0006 0.0002 0 0.0019 0.0001 0.017 0.0001 0.0009 0.0002 0.0001 0.0017
Bud 0.0004 0.0004 0.0006 0.0001 0.0003 0.0004 0.0006 0.0046 0.0041 0.0007 0.0047 0.0008 0.0005 0.0016 0.0007 0.0008 0.0024 0.0018
Bud Neck 0.0003 0.0005 0.0107 0.0003 0.0006 0.0019 0.0059 0.0016 0.0017 0.0005 0.0007 0.0048 0.0021 0.0002 0.0014 0.0008 0.0003 0.0031
Bud Periphery 0.0011 0.0004 0.0011 0.0002 0.0005 0.0007 0.0008 0.0047 0.0045 0.0007 0.0057 0.0008 0.0008 0.0016 0.0008 0.0018 0.0079 0.0053
Bud Site 0.0009 0.0062 0.0049 0.0002 0.0007 0.0008 0.0018 0.0145 0.0065 0.0004 0.0007 0.0009 0.0019 0.0013 0.0013 0.0017 0.0008 0.0006
Cell Periphery 0.0015 0.001 0.0009 0.0002 0.0006 0.0004 0.0003 0.0009 0.0006 0.0001 0.0003 0.0001 0.0008 0.0006 0.0003 0.0008 0.0011 0.0004
Cytoplasm 0.1565 0.3312 0.3259 0.1577 0.3109 0.3661 0.1086 0.1592 0.133 0.1502 0.0907 0.1785 0.0621 0.1879 0.0907 0.1323 0.1372 0.1434
Cytoplasmic Foci 0.0386 0.0505 0.0568 0.0431 0.0608 0.0448 0.0718 0.0627 0.0787 0.0617 0.0491 0.0622 0.0669 0.0569 0.0856 0.1093 0.0618 0.0947
Eisosomes 0.0005 0.0001 0.0001 0.0001 0 0 0 0 0 0 0.0001 0 0.0005 0.0001 0.0008 0.0001 0 0.0001
Endoplasmic Reticulum 0.0208 0.0142 0.0141 0.0075 0.0118 0.0168 0.0062 0.0074 0.0072 0.0028 0.0033 0.0036 0.0069 0.0095 0.0071 0.0053 0.0044 0.0052
Endosome 0.3055 0.2088 0.1964 0.2885 0.2613 0.2667 0.4435 0.3417 0.3609 0.3611 0.5017 0.408 0.3942 0.2439 0.4025 0.3582 0.357 0.3408
Golgi 0.0036 0.0053 0.0034 0.0024 0.0059 0.0043 0.0041 0.0054 0.0059 0.0031 0.006 0.004 0.0084 0.005 0.0125 0.0048 0.0051 0.0096
Lipid Particles 0.0134 0.011 0.0263 0.0138 0.0129 0.0059 0.0104 0.0064 0.0105 0.0023 0.0066 0.0018 0.0155 0.0091 0.0111 0.0181 0.0083 0.0088
Mitochondria 0.0079 0.0018 0.0028 0.0025 0.0093 0.0117 0.0041 0.0061 0.0114 0.0047 0.0066 0.0048 0.0112 0.0145 0.0253 0.0052 0.0047 0.0193
None 0.1444 0.1685 0.1746 0.2265 0.1667 0.0889 0.0642 0.1001 0.1129 0.0329 0.0525 0.0293 0.1282 0.3371 0.2311 0.1856 0.1703 0.2079
Nuclear Periphery 0.0118 0.0119 0.0076 0.0072 0.007 0.0094 0.0168 0.0116 0.0122 0.0048 0.0088 0.0057 0.0452 0.0076 0.0052 0.0034 0.0035 0.005
Nucleolus 0.0019 0.0007 0.0016 0.0005 0.0004 0.0005 0.0012 0.0028 0.0038 0.0006 0.0012 0.0018 0.0034 0.0009 0.0013 0.0021 0.0014 0.0007
Nucleus 0.0049 0.0042 0.0057 0.0026 0.0025 0.0041 0.0082 0.0081 0.0106 0.0042 0.0044 0.0134 0.0079 0.0038 0.0024 0.0042 0.0031 0.0024
Peroxisomes 0.0005 0.0007 0.0018 0.0007 0.0008 0.0007 0.0025 0.0013 0.002 0.0008 0.0016 0.0004 0.0052 0.0006 0.0049 0.0029 0.0009 0.0052
Punctate Nuclear 0.0014 0.0011 0.0038 0.0006 0.0006 0.0011 0.0021 0.0025 0.006 0.0009 0.0022 0.0015 0.0219 0.0008 0.0012 0.0039 0.0003 0.0028
Vacuole 0.2478 0.1603 0.1392 0.2002 0.1294 0.1378 0.2072 0.2083 0.1893 0.2793 0.1914 0.2238 0.1767 0.0943 0.09 0.1379 0.2064 0.1161
Vacuole Periphery 0.0362 0.0212 0.0213 0.0451 0.0171 0.0369 0.04 0.0496 0.038 0.0882 0.0599 0.0536 0.0227 0.0225 0.0229 0.0207 0.023 0.0252

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.5777 9.5551 11.0899 13.1609 7.8176 8.2175 13.0673 14.4624 13.8766 11.3362
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –
Standard name
Human Ortholog
Description Subunit of SEACAT, a subcomplex of the SEA complex; Rtc1p, along with Mtc5p and Sea4p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; null suppresses cdc13-1
Localization
Cell Percentages cytoplasm (13%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rtc1

Rtc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rtc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available