Standard name
Human Ortholog
Description 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; restores resistance to glycolaldehyde by coupling reduction of glycolaldehyde to ethylene glycol and oxidation of NADPH to NADP+; protein abundance increases in response to DNA replication stress; methylglyoxal reductase (NADPH-dependent) is also known as D-lactaldehyde dehydrogenase

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.98 0.98 0.93 0.84 0.77 0.81 0.69 0.71 0.65 0.7 0.97 0.96 0.97 0.93 0.91 0.93 0.89 0.9 0.93 0.89 0.88 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.12 0.05 0.29 0.17 0.28 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0.1 0.18 0.13 0.12 0.2 0.13 0.14 0 0 0 0.08 0.13 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 1 0 0 0 3 0 0 0 0 0 0 0 1 1 1 1 3
Bud 0 2 1 0 0 1 1 1 6 3 19 10 0 1 0 0 0 0 12 5 5 4 12 19
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2 1 4 8 7 3 4 4 5 5 0 2 5 12 11 5 0 0 0 0 0 0
Cytoplasm 281 203 289 298 452 317 361 347 192 235 225 248 323 548 600 289 324 260 259 181 270 251 422 413
Endoplasmic Reticulum 0 1 0 0 1 4 6 7 3 5 1 0 3 3 3 4 6 4 1 1 1 0 5 3
Endosome 0 1 0 0 0 0 0 0 0 1 0 0 1 2 2 0 1 1 3 0 1 1 2 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 2 2
Mitochondria 4 2 0 1 6 34 54 22 80 57 96 57 0 0 3 2 0 1 0 0 2 2 4 0
Nucleus 2 1 0 2 4 4 5 14 2 9 10 9 2 9 3 1 1 2 1 3 2 0 2 1
Nuclear Periphery 0 0 0 0 1 1 0 0 0 4 2 0 0 0 0 0 0 0 0 0 1 0 2 1
Nucleolus 0 0 0 0 1 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 3 5 8
Vac/Vac Membrane 0 2 6 7 35 37 85 54 34 68 45 48 11 22 17 25 46 17 6 4 1 8 9 7
Unique Cell Count 285 208 295 305 485 376 466 430 278 333 344 353 334 568 621 312 355 279 293 203 292 282 478 475
Labelled Cell Count 287 212 299 309 504 406 520 450 322 386 403 380 340 587 633 334 390 290 293 203 292 282 478 475


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 10.4 8.1 8.1 8.3 7.1 7.2 7.9 6.9 7.8 7.2 8.2 12.9 13.0 13.2 19.1 21.1 20.2 7.8 8.1 8.3
Std Deviation (1e-4) 2.5 3.7 2.1 2.5 2.3 1.9 2.0 2.3 2.5 2.9 3.7 3.6 5.8 5.4 6.0 7.6 11.1 8.0 2.2 2.5 2.3
Intensity Change (Log2) 0.01 0.03 -0.18 -0.17 -0.04 -0.24 -0.06 -0.17 0.01 0.67 0.68 0.7 1.23 1.38 1.32 -0.06 -0.01 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 3.8 2.4 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.6 2.2 0
Cytoplasm -0.2 -3.0 -5.9 -7.8 -7.0 -9.4 -9.2 -10.3 -9.3 -1.0 -1.2 -1.1 -3.1 -3.7 -2.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.3 6.1 3.9 9.9 7.5 9.8 7.2 0 0 0 0 0 0
Nucleus 0 0 0 0 3.1 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.2 3.1 4.1 6.7 5.1 4.8 7.1 5.1 5.3 1.0 1.4 0.6 3.3 5.1 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.1332 9.1052 9.0664 8.3436 8.0112 8.7586 5.9889 7.8352 7.3037 7.0082 5.9269 7.8721 9.1817 12.4018 11.1871 11.9348 8.3251 10.8902
Actin 0.0068 0.0012 0.0021 0.0019 0.0015 0.0015 0.0243 0.0052 0.003 0.001 0.0048 0.0011 0.0021 0.0019 0.0006 0.0003 0.0022 0.0006
Bud 0.0018 0.0063 0.0026 0.0115 0.004 0.0008 0.0022 0.0037 0.0036 0.0008 0.0282 0.0003 0.0003 0.0032 0.0008 0.001 0.015 0.0015
Bud Neck 0.002 0.0005 0.0011 0.0032 0.0014 0.0017 0.0088 0.0005 0.0005 0.001 0.0013 0.0019 0.0004 0.0004 0.0005 0.0005 0.0008 0.0015
Bud Periphery 0.0009 0.0027 0.0014 0.0053 0.001 0.0004 0.0019 0.0014 0.0016 0.0021 0.012 0.0002 0.0002 0.0012 0.0004 0.0004 0.0167 0.0012
Bud Site 0.0049 0.0059 0.0026 0.0044 0.0012 0.0003 0.0049 0.0152 0.0021 0.0004 0.0027 0.0002 0.0011 0.0027 0.002 0.0003 0.0016 0.0002
Cell Periphery 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0.0003 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.8586 0.918 0.9159 0.8417 0.8357 0.8993 0.8101 0.9278 0.9122 0.8856 0.8252 0.9085 0.9479 0.9511 0.9603 0.9175 0.9229 0.9433
Cytoplasmic Foci 0.0151 0.004 0.0065 0.0063 0.0175 0.0118 0.0118 0.0046 0.0058 0.004 0.0075 0.0085 0.0044 0.0047 0.0023 0.0029 0.0023 0.0028
Eisosomes 0.0001 0 0 0 0.0001 0 0.0003 0 0 0.0001 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0054 0.002 0.0024 0.0031 0.0039 0.0034 0.0088 0.0015 0.0019 0.0032 0.0024 0.0025 0.0027 0.0018 0.0016 0.0011 0.0021 0.0024
Endosome 0.0108 0.0024 0.0026 0.0028 0.0035 0.0059 0.0242 0.0012 0.0035 0.0011 0.0082 0.0041 0.003 0.001 0.001 0.0278 0.0013 0.0018
Golgi 0.001 0.0002 0.0002 0.0003 0.0003 0.0002 0.0044 0.0003 0.0004 0.0001 0.007 0.0002 0.0007 0.0001 0.0001 0.0022 0.0001 0
Lipid Particles 0.0007 0 0.0001 0.0001 0.0004 0.0001 0.0024 0 0.0001 0.0001 0.0161 0.0001 0.0012 0 0 0.0006 0 0
Mitochondria 0.0008 0.0005 0.0005 0.0014 0.0046 0.0009 0.0045 0.0003 0.0009 0.0008 0.0066 0.0004 0.0009 0.0003 0.0002 0.0121 0.0003 0.0002
None 0.0292 0.0074 0.0096 0.0235 0.0106 0.0119 0.0454 0.0149 0.0437 0.0749 0.0442 0.0337 0.0083 0.0058 0.0051 0.0047 0.0038 0.0027
Nuclear Periphery 0.0092 0.0041 0.0049 0.0074 0.0105 0.0059 0.0186 0.002 0.0024 0.0019 0.0032 0.0066 0.0039 0.0028 0.0029 0.0015 0.0034 0.0038
Nucleolus 0.0004 0.0002 0.0001 0.0018 0.0022 0.0002 0.0007 0.0001 0.0001 0.0001 0.0007 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0418 0.0387 0.0387 0.0732 0.0836 0.0469 0.014 0.0168 0.0149 0.0197 0.0205 0.0266 0.0186 0.0179 0.0198 0.01 0.0238 0.0347
Peroxisomes 0.0012 0.0002 0.002 0.0004 0.0008 0.0002 0.0022 0.0017 0.0003 0.0002 0.0021 0.0003 0.0002 0.0021 0.0001 0.0002 0.0001 0
Punctate Nuclear 0.0055 0.0021 0.0046 0.0074 0.0132 0.0061 0.0053 0.0014 0.0015 0.0009 0.0032 0.0023 0.0014 0.001 0.001 0.0002 0.0012 0.0012
Vacuole 0.0032 0.003 0.0015 0.0034 0.0034 0.002 0.0038 0.0011 0.0011 0.0013 0.0027 0.0015 0.0019 0.0015 0.0012 0.01 0.0019 0.0016
Vacuole Periphery 0.0005 0.0003 0.0003 0.0006 0.0005 0.0004 0.0012 0.0001 0.0003 0.0003 0.0011 0.0004 0.0003 0.0002 0.0002 0.0064 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 48.2538 42.1774 39.8331 44.6042 34.0622 56.8008 51.6108 46.4451 51.3903 39.5074
Translational Efficiency 1.1326 1.3758 1.5938 1.2703 1.2614 1.2564 1.5011 1.7474 1.4771 1.5217

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; restores resistance to glycolaldehyde by coupling reduction of glycolaldehyde to ethylene glycol and oxidation of NADPH to NADP+; protein abundance increases in response to DNA replication stress; methylglyoxal reductase (NADPH-dependent) is also known as D-lactaldehyde dehydrogenase
Localization
Cell Percentages cytoplasm (82%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gre2

Gre2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gre2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available