Standard name
Human Ortholog
Description Nuclear exosome exonuclease component; has 3'-5' exonuclease activity that is regulated by Lrp1p; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; role in sn/snoRNAs precursor degradation; forms a stable heterodimer with Lrp1p; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.12 0 0.1 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.12 0 0 0 0.06 0.12 0.07 0.29 0.27 0.37 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.77 0.71 0.89 0.93 0.93 0.89 0.85 0.86 0.83 0.79 0.75 0.79 0.7 0.66 0.7 0.78 0.75 0.74 0.57 0.62 0.68 0.46 0.29 0.28
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Nucleolus 0.3 0.32 0.09 0 0 0.07 0.07 0.05 0.07 0.09 0.08 0.09 0.28 0.27 0.22 0.11 0.1 0.09 0.36 0.28 0.22 0.26 0.35 0.29
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.12 0 0.05 0.05 0.22 0.27 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 1 0 6 4 3 4 5 6 8 0 0 0 0 0 0 3 2 1 2 6 4
Bud Neck 0 0 1 0 1 1 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0 0 1 4
Bud Site 0 0 0 0 0 6 5 6 14 10 9 12 1 2 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 5 6 14 8 7 18 16 7 15 12 10 21 34 52 10 17 7 0 5 0 0 1 0
Endoplasmic Reticulum 0 1 0 1 0 0 0 1 0 0 0 0 1 0 1 3 5 3 0 0 1 0 1 0
Endosome 1 1 1 2 0 0 0 0 1 0 0 2 2 2 3 5 1 1 2 1 4 1 3 10
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 1 0 2
Mitochondria 2 42 0 4 4 38 70 43 151 149 213 95 7 2 3 1 3 1 1 1 2 2 6 7
Nucleus 172 252 359 348 308 551 490 509 436 442 427 377 251 230 304 204 122 125 142 237 320 75 95 88
Nuclear Periphery 0 1 1 4 2 16 3 5 11 14 3 9 8 5 7 7 1 3 0 0 3 0 3 15
Nucleolus 67 112 38 7 9 44 40 28 39 51 44 45 102 92 93 29 17 15 89 107 103 42 115 94
Peroxisomes 0 0 0 0 0 0 0 2 0 0 0 0 1 5 2 0 0 0 0 0 0 0 0 1
SpindlePole 0 1 0 2 0 8 1 7 4 7 1 10 3 10 0 0 0 1 0 2 3 1 3 9
Vac/Vac Membrane 6 11 12 5 10 14 11 17 4 5 15 10 4 9 13 22 10 21 8 20 21 35 89 78
Unique Cell Count 223 353 405 375 330 618 579 592 527 561 571 479 360 347 432 261 162 168 249 383 469 164 331 321
Labelled Cell Count 252 427 418 388 342 691 642 638 672 700 730 580 401 391 478 281 177 177 249 383 469 164 331 321


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 7.6 8.6 7.8 7.5 6.0 6.0 6.2 5.2 5.1 4.8 5.4 9.1 8.7 8.4 11.1 11.6 10.8 8.6 8.4 8.6
Std Deviation (1e-4) 1.7 1.5 1.3 1.6 1.3 1.1 1.1 1.2 1.1 1.1 1.2 1.0 2.8 2.7 2.4 2.2 2.7 2.7 1.6 1.8 1.7
Intensity Change (Log2) -0.15 -0.2 -0.52 -0.53 -0.47 -0.74 -0.77 -0.84 -0.67 0.08 0.02 -0.03 0.36 0.43 0.33 -0.01 -0.04 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.3 0 0 3.2 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 0.9 -0.5 1.6 1.3 -0.2 1.3 0.7 0.7 3.3 5.1 6.0 1.9 4.9 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.1 7.3 5.5 11.8 11.3 0 9.5 0 0 0 0 0 0
Nucleus 2.0 2.2 0.3 -1.8 -1.2 -2.5 -4.0 -5.4 -3.9 -6.5 -7.4 -6.5 -3.7 -4.0 -4.3
Nuclear Periphery 0 0 2.9 0 0 2.5 2.8 0 0 0 0 0 0 0 0
Nucleolus -4.5 -3.7 -1.3 -1.4 -2.9 -1.1 -0.2 0 0 6.8 6.2 4.8 0.7 0.4 -0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.6 0.1 -0.7 -1.1 -0.1 -2.6 -2.4 -0.3 -0.8 -1.8 -0.3 0 3.1 1.8 4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0645 4.8449 4.5165 3.1471 3.2569 3.9287 6.5218 6.5777 6.3982 5.5991 5.9302 5.9092 5.0179 5.1157 4.6819 4.6329 4.8632 4.8489
Actin 0.0104 0 0.0001 0 0.0032 0 0.0012 0 0.0041 0.0162 0.0144 0 0.005 0 0.0065 0.0119 0.0502 0.0046
Bud 0.0004 0 0.0001 0 0.0001 0.0001 0.0002 0 0.0002 0.0003 0.0001 0 0.0003 0 0.0002 0.0002 0.0005 0.0001
Bud Neck 0.0006 0.0003 0.0002 0.0002 0.0005 0.0011 0.0008 0.0002 0.0004 0.0009 0.0014 0.0006 0.0015 0.0001 0.0005 0.0003 0.0016 0.0008
Bud Periphery 0.0012 0.0001 0.0002 0.0001 0.0008 0.0003 0.0009 0 0.0003 0.0005 0.0003 0.0001 0.001 0.0001 0.0005 0.0002 0.0012 0.0001
Bud Site 0.0011 0.0001 0.0002 0.0001 0.0003 0.0002 0.0006 0.0001 0.0006 0.0015 0.0002 0.0001 0.001 0.0001 0.001 0.0007 0.0012 0.0001
Cell Periphery 0.0003 0.0001 0.0002 0 0.0008 0.0002 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0001 0.0004 0 0.0002 0
Cytoplasm 0.0018 0.0003 0.0003 0 0.0015 0 0.0001 0 0.0006 0.0003 0.0001 0 0.0009 0 0.0003 0.0012 0.001 0.0001
Cytoplasmic Foci 0.0115 0 0.0077 0 0.0012 0.0001 0.0011 0 0.0029 0.003 0.0034 0.0001 0.0033 0 0.0016 0.0091 0.0045 0.0001
Eisosomes 0.0001 0 0 0 0.0004 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0002 0.0001 0.0004 0
Endoplasmic Reticulum 0.0022 0 0.0001 0 0.0008 0 0.0007 0 0.0012 0.0006 0.0003 0 0.0012 0 0.001 0.0005 0.0022 0.0003
Endosome 0.0072 0.0001 0.0013 0 0.0033 0.0001 0.0053 0 0.005 0.0114 0.0035 0.0005 0.0031 0 0.0025 0.0057 0.0218 0.0009
Golgi 0.0014 0 0.0002 0 0.0005 0.0001 0.002 0 0.0017 0.0109 0.0029 0.0001 0.0011 0 0.0006 0.002 0.0093 0.0004
Lipid Particles 0.0072 0 0.002 0 0.002 0.0015 0.0083 0 0.0064 0.006 0.0197 0.0003 0.0118 0 0.0047 0.003 0.0127 0.0002
Mitochondria 0.0025 0.0001 0.0009 0.0002 0.0108 0.0008 0.0154 0.0001 0.0067 0.0028 0.0014 0.0004 0.0053 0.0001 0.0042 0.0005 0.0109 0.0002
None 0.0015 0 0.0001 0 0.0148 0 0.0002 0 0.0003 0.0002 0.0005 0 0.0006 0 0.0005 0.0031 0.0017 0.0001
Nuclear Periphery 0.0081 0.0058 0.0055 0.0081 0.0204 0.0044 0.0073 0.0029 0.009 0.0016 0.0066 0.0002 0.0058 0.0004 0.0062 0.0023 0.0366 0.0018
Nucleolus 0.4032 0.3397 0.4857 0.379 0.3842 0.4481 0.3735 0.4367 0.467 0.356 0.301 0.4661 0.3975 0.4125 0.4363 0.4327 0.4242 0.4796
Nucleus 0.5174 0.6462 0.4915 0.6095 0.5114 0.5389 0.5684 0.5557 0.4847 0.5819 0.6131 0.5284 0.5458 0.5839 0.5267 0.5078 0.3971 0.5082
Peroxisomes 0.0103 0 0.0002 0 0.0007 0.0001 0.0019 0 0.0035 0.0016 0.003 0 0.0032 0 0.0007 0.0067 0.0056 0
Punctate Nuclear 0.0075 0.0018 0.0013 0.0015 0.0167 0.002 0.003 0.0036 0.0033 0.0029 0.0257 0.0013 0.0066 0.0013 0.0037 0.0118 0.0125 0.0021
Vacuole 0.0029 0.0046 0.0009 0.0003 0.0087 0.001 0.0053 0.0004 0.0013 0.0005 0.0008 0.0012 0.003 0.0006 0.0013 0.0002 0.0019 0.0002
Vacuole Periphery 0.0013 0.0008 0.0011 0.0008 0.0169 0.001 0.0033 0.0002 0.0009 0.0004 0.0012 0.0004 0.0013 0.0006 0.0005 0.0001 0.0029 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.8346 39.8672 34.5691 39.5039 29.8856 29.5481 36.4598 30.5689 31.7538 35.0856
Translational Efficiency 0.7605 0.95 1.0063 0.7731 0.8622 0.8588 0.8141 0.8048 0.8333 0.7957

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Nuclear exosome exonuclease component; has 3'-5' exonuclease activity that is regulated by Lrp1p; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; role in sn/snoRNAs precursor degradation; forms a stable heterodimer with Lrp1p; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based
Localization
Cell Percentages nucleus (74%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rrp6

Rrp6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rrp6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available