Standard name
Human Ortholog
Description Component of the small subunit processome; involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.07 0.08 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0.06 0.07 0 0 0 0 0 0 0 0.11 0.1 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0
Endosome 0 0 0.07 0 0 0.05 0.07 0.1 0 0 0 0 0 0.05 0.05 0.11 0.12 0.1 0 0.05 0 0 0 0.05
Golgi 0 0 0.13 0.11 0 0 0 0 0 0 0 0 0.32 0.34 0.26 0.1 0.39 0.12 0.05 0.08 0.05 0.08 0.08 0.08
Mitochondria 0.93 0.9 0.8 0.9 0.83 0.79 0.73 0.73 0.92 0.88 0.92 0.93 0.8 0.73 0.7 0.06 0.08 0.08 0.81 0.75 0.77 0.76 0.72 0.72
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0.06 0.07 0.09 0.08 0.11 0.07 0.06 0.07 0.07 0 0 0 0 0.08 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.08 0.08 0.08 0.07 0.09 0.06 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0.31 0.14 0.11 0 0 0.09 0.05 0.07 0
SpindlePole 0 0 0.06 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0.12 0.06 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.21 0.07 0.05 0.06 0.11 0.1 0.1 0 0.06 0.05 0 0.13 0.08 0.15 0.11 0.13 0.21 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 2 1 0 0 1 1 0 3 1 0 0 3 1 0 1 2 4 10 2 0 4 3
Bud 1 5 5 5 4 1 11 6 6 8 6 6 2 0 1 9 4 3 0 2 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 1 4 0 0 0 1 1 0 2 3 2 3 0 0 0 3 1 3 – – – – – –
Cell Periphery 6 31 6 3 1 6 4 2 1 5 2 2 5 7 5 13 9 10 0 1 0 0 0 0
Cytoplasm 0 11 4 5 2 12 19 20 8 6 3 2 3 6 11 12 13 10 0 1 1 0 0 0
Endoplasmic Reticulum 3 4 2 2 2 1 1 0 2 1 2 2 1 4 10 4 3 13 0 1 0 0 0 2
Endosome 5 21 12 7 1 14 22 29 2 6 4 6 1 10 12 12 15 13 11 31 6 5 6 13
Golgi 11 17 23 18 0 4 3 8 0 2 4 1 46 76 69 11 49 16 20 50 10 14 19 19
Mitochondria 350 567 144 151 72 204 220 218 279 275 345 325 115 163 184 7 10 11 310 484 158 130 184 180
Nucleus 2 3 1 0 2 5 8 6 1 5 6 5 0 0 1 0 4 7 0 1 0 0 1 0
Nuclear Periphery 16 14 4 3 5 19 27 25 34 22 24 24 10 2 10 5 5 10 4 5 1 0 3 3
Nucleolus 10 14 3 3 7 20 25 22 26 18 25 21 1 2 2 4 0 5 1 2 0 1 1 1
Peroxisomes 5 21 10 1 0 2 7 4 4 3 4 6 7 13 9 35 18 15 15 11 17 9 18 8
SpindlePole 2 5 11 1 4 3 15 8 7 12 5 7 2 3 6 13 7 14 2 0 1 2 1 1
Vac/Vac Membrane 51 136 13 8 5 27 31 29 12 19 18 12 19 17 39 12 16 28 9 37 3 5 10 9
Unique Cell Count 375 633 179 168 87 257 302 297 302 312 373 348 144 222 261 113 127 132 382 646 206 173 255 251
Labelled Cell Count 464 857 240 208 105 319 395 378 384 388 451 422 212 306 360 140 155 160 382 646 206 173 255 251


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 7.7 7.9 7.7 5.6 7.6 7.3 7.7 6.0 6.7 6.4 6.1 8.6 8.4 7.8 6.6 8.0 7.6 9.8 10.0 10.0
Std Deviation (1e-4) 1.6 1.3 1.3 1.3 1.2 1.7 1.7 1.6 1.3 1.4 1.5 1.3 1.5 1.5 1.4 1.7 2.2 2.0 2.3 2.3 1.9
Intensity Change (Log2) – – – -0.04 -0.49 -0.06 -0.12 -0.04 -0.4 -0.24 -0.3 -0.37 0.12 0.09 -0.02 -0.26 0.02 -0.04 0.31 0.34 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.5 0 0 0 0 0 0 0 0 2.0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 -0.1 0 2.8 1.5 1.7
Cytoplasm 0 0 1.3 2.0 2.2 0 0 0 0 0 0 1.1 3.1 3.0 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.6
Endosome -1.0 0 -0.5 0.2 1.2 -3.8 -2.7 -3.7 -3.0 -2.7 -1.0 -1.0 1.2 1.6 1.0
Golgi -0.6 -3.5 -4.8 -5.6 -4.3 -6.4 -5.9 -6.0 -6.6 4.2 4.9 3.4 -0.8 5.2 -0.2
Mitochondria 2.5 0.5 -0.3 -1.9 -1.7 3.9 2.3 4.2 4.5 -0.1 -1.7 -2.4 -12.4 -12.5 -12.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.4 2.9 2.7 3.5 2.3 2.1 2.3 2.1 0 0.9 0 0 2.2
Nucleolus 0 0 2.8 3.0 2.7 3.1 2.2 2.5 2.3 0 0 0 0 0 0
Peroxisomes -2.7 0 0 -1.9 -2.7 -2.7 0 0 -2.4 -0.3 0.1 -1.1 5.9 2.6 1.9
SpindlePole -2.8 0 -2.9 -0.6 -1.9 -2.1 -1.2 -3.1 -2.5 -2.2 -2.6 -2.1 1.6 -0.2 1.4
Vacuole -1.0 -0.5 1.2 1.1 0.9 -1.6 -0.5 -1.2 -2.0 1.8 0.1 2.5 1.0 1.6 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6081 4.2481 4.2884 3.9325 3.7558 4.6545 9.5179 9.9732 9.6153 9.147 9.1517 10.3324 7.312 7.1276 7.1251 6.6852 6.5865 7.3326
Actin 0.0237 0.0039 0.0093 0.0278 0.0069 0.0022 0.0177 0.0076 0.0038 0.0017 0.056 0.0127 0.0214 0.0189 0.0123 0.0158 0.0007 0.0021
Bud 0.0003 0.0003 0.0001 0.0001 0.0002 0 0.0001 0.0003 0.0001 0.0012 0.0001 0.0002 0.0004 0.0004 0.0002 0.0002 0.0001 0.0001
Bud Neck 0.0011 0.0002 0.0009 0.0012 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001 0.0003 0.0008 0.0003 0.0003 0.0002 0.0008 0 0.0001
Bud Periphery 0.0005 0.0004 0.0002 0.0001 0.0003 0.0001 0.0003 0.0004 0.0002 0.0032 0.0001 0.0006 0.0009 0.0006 0.0006 0.0003 0.0003 0.0002
Bud Site 0.0008 0.0008 0.0016 0.0002 0.0003 0.0001 0.0005 0.0008 0.0002 0.0008 0.0003 0.0008 0.0009 0.0026 0.0006 0.0016 0.0001 0.0001
Cell Periphery 0.0002 0.0002 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0003 0.0037 0.0001 0.0001 0.0001
Cytoplasm 0.0002 0.0002 0.0004 0 0.0002 0 0.0012 0.0001 0.0001 0.0004 0 0.0001 0.0014 0.0001 0.0015 0 0.0002 0.0002
Cytoplasmic Foci 0.0062 0.0069 0.0029 0.0037 0.0039 0.0029 0.0268 0.0067 0.0012 0.0131 0.0043 0.001 0.008 0.0056 0.0099 0.0008 0.002 0.0009
Eisosomes 0.0006 0.0002 0.0002 0.0001 0.0002 0.0001 0.0007 0.0002 0.0001 0.0001 0.0008 0.0003 0.0006 0.0003 0.0003 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.0012 0.0001 0.0001 0 0.0003 0 0.0011 0 0.0003 0.0007 0 0.0002 0.0023 0.0001 0.0005 0 0.0002 0.0008
Endosome 0.0206 0.0172 0.0045 0.0031 0.0216 0.0135 0.0147 0.0113 0.0144 0.0566 0.0044 0.0038 0.0188 0.0155 0.0165 0.0007 0.0054 0.0079
Golgi 0.0298 0.0462 0.0105 0.0089 0.0353 0.0293 0.0102 0.0167 0.0099 0.0123 0.0113 0.0109 0.0195 0.0502 0.0212 0.0016 0.0086 0.023
Lipid Particles 0.0177 0.0157 0.0104 0.0033 0.0052 0.0054 0.0157 0.0081 0.0121 0.0502 0.0113 0.0037 0.0114 0.0104 0.0043 0.0033 0.0311 0.0024
Mitochondria 0.8725 0.8939 0.9266 0.9251 0.9197 0.9333 0.8848 0.931 0.9473 0.8247 0.8595 0.9463 0.8802 0.8696 0.9049 0.9667 0.9012 0.954
None 0.0013 0 0.0044 0 0.0001 0 0.0014 0 0 0.0003 0 0.0003 0.0006 0.0001 0.0004 0.0001 0.0001 0.0001
Nuclear Periphery 0.0011 0.0001 0.0001 0 0.0001 0 0.0019 0 0.0014 0.002 0 0.0007 0.0016 0.0001 0.0019 0 0.0132 0.0013
Nucleolus 0.0002 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0 0.0003 0 0.0036 0.0002 0.0002 0.0005 0.0001 0.0003 0
Nucleus 0.0002 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0.0002 0 0.0009 0.0004 0.0001 0.0009 0 0.0161 0.0002
Peroxisomes 0.0152 0.0105 0.0225 0.0257 0.0029 0.0114 0.0171 0.0146 0.006 0.0263 0.051 0.0061 0.0165 0.0199 0.0146 0.0069 0.0133 0.0035
Punctate Nuclear 0.0004 0 0.0006 0 0 0 0.0029 0 0.0001 0.0012 0 0.002 0.0064 0.0001 0.0019 0.0001 0.0002 0.0001
Vacuole 0.0021 0.0013 0.0012 0.0001 0.001 0.0004 0.0008 0.0009 0.0009 0.0022 0.0001 0.0016 0.0048 0.0025 0.0012 0.0001 0.0019 0.0007
Vacuole Periphery 0.0041 0.0016 0.0031 0.0003 0.0013 0.0009 0.0016 0.0007 0.0017 0.0022 0.0002 0.003 0.0029 0.0023 0.0019 0.0002 0.0047 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 57.6944 48.9965 49.7524 28.0548 41.1418 46.1299 32.9182 21.1177 25.4913 37.5747
Translational Efficiency 2.3101 2.6646 1.5724 2.2072 2.112 1.6478 1.8013 2.4491 1.4079 1.8123

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the small subunit processome; involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Utp23

Utp23


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Utp23-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available