Standard name | |
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Human Ortholog | |
Description | Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; similar to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 1 | 2 | 13 | 8 | 12 | 13 | 7 | 19 | 1 | 0 | 0 | 4 | 3 | 4 | 1 | 0 | 0 | 0 | 1 | 3 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 2 | 3 | 7 | 1 | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 0 | 3 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 0 | 2 | 16 | 25 | 21 | 34 | 13 | 19 | 44 | 15 | 38 | 8 | 9 | 6 | 46 | 32 | 32 | 0 | 0 | 0 | 1 | 0 | 2 |
Cytoplasm | 193 | 17 | 85 | 173 | 264 | 228 | 263 | 256 | 202 | 254 | 160 | 192 | 138 | 148 | 168 | 444 | 356 | 428 | 183 | 53 | 98 | 67 | 86 | 105 |
Endoplasmic Reticulum | 1 | 0 | 3 | 14 | 20 | 18 | 46 | 44 | 32 | 50 | 15 | 40 | 3 | 4 | 0 | 13 | 9 | 14 | 4 | 0 | 3 | 3 | 2 | 6 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Golgi | 2 | 0 | 1 | 1 | 2 | 1 | 3 | 1 | 2 | 0 | 0 | 3 | 0 | 0 | 0 | 19 | 16 | 9 | 0 | 0 | 0 | 0 | 2 | 8 |
Mitochondria | 0 | 0 | 0 | 0 | 2 | 1 | 10 | 4 | 16 | 13 | 22 | 10 | 0 | 0 | 0 | 3 | 6 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 3 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 0 | 1 | 10 | 7 | 3 | 15 | 7 | 13 | 15 | 5 | 14 | 0 | 0 | 0 | 13 | 4 | 4 | 0 | 0 | 0 | 0 | 2 | 1 |
Unique Cell Count | 196 | 17 | 89 | 199 | 300 | 258 | 345 | 320 | 278 | 338 | 211 | 273 | 143 | 150 | 168 | 492 | 387 | 451 | 192 | 57 | 109 | 77 | 100 | 139 |
Labelled Cell Count | 203 | 17 | 93 | 215 | 322 | 275 | 388 | 339 | 298 | 394 | 231 | 319 | 150 | 161 | 174 | 549 | 431 | 500 | 192 | 57 | 109 | 77 | 100 | 139 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 20.7 | 23.9 | 19.9 | 21.4 | 21.7 | 20.4 | 19.2 | 18.1 | 17.1 | 17.2 | 15.5 | 16.5 | 27.6 | 26.1 | 26.1 | 27.7 | 31.4 | 30.4 | 21.3 | 22.4 | 23.8 |
Std Deviation (1e-4) | 3.7 | 3.0 | 3.6 | 4.3 | 4.8 | 4.0 | 4.0 | 4.1 | 4.1 | 4.2 | 5.0 | 4.7 | 6.1 | 4.8 | 5.0 | 6.9 | 7.7 | 6.6 | 6.3 | 4.6 | 5.8 |
Intensity Change (Log2) | – | – | – | 0.1 | 0.13 | 0.03 | -0.06 | -0.14 | -0.23 | -0.21 | -0.36 | -0.27 | 0.47 | 0.39 | 0.39 | 0.48 | 0.66 | 0.61 | 0.09 | 0.17 | 0.26 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.9 | 2.0 | 1.9 | 2.3 | 0 | 1.6 | 2.9 | 1.7 | 3.1 | 0 | 0 | 0 | 2.2 | 2.0 | 1.7 |
Cytoplasm | -2.2 | -2.0 | -2.0 | -4.1 | -3.5 | -4.5 | -4.2 | -4.0 | -4.9 | 0.4 | 1.5 | 2.8 | -1.6 | -1.1 | -0.2 |
Endoplasmic Reticulum | 1.2 | 1.2 | 1.2 | 2.6 | 2.7 | 2.3 | 2.9 | 1.2 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 17.1088 | 24.6371 | 21.5454 | 20.2858 | 17.6715 | 21.8235 | 20.225 | 26.3117 | 24.1622 | 22.0193 | 21.4336 | 22.8399 | 19.0616 | 24.5507 | 22.4691 | 21.1236 | 14.9478 | 22.3352 |
Actin | 0.0092 | 0.0002 | 0.0052 | 0.0219 | 0.0028 | 0.0015 | 0.0017 | 0.0002 | 0.0003 | 0 | 0.0004 | 0.0016 | 0.0062 | 0.0034 | 0.0125 | 0.0008 | 0.0019 | 0.0005 |
Bud | 0.0007 | 0.0001 | 0.0004 | 0.0002 | 0.0012 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0069 | 0.0003 |
Bud Neck | 0.0006 | 0.0002 | 0.0007 | 0.0006 | 0.0005 | 0.0009 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0006 | 0.0008 | 0.0004 | 0.0001 | 0.0005 | 0.0002 | 0.0009 | 0.0006 |
Bud Periphery | 0.001 | 0 | 0.0004 | 0.0001 | 0.0031 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0077 | 0.0001 |
Bud Site | 0.0015 | 0.0002 | 0.0021 | 0.001 | 0.0007 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0014 | 0.0001 | 0.0005 | 0.0001 | 0.0043 | 0.0001 |
Cell Periphery | 0.0006 | 0.0001 | 0.0004 | 0.0001 | 0.0017 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0 |
Cytoplasm | 0.8207 | 0.9795 | 0.9153 | 0.8856 | 0.7473 | 0.9321 | 0.9297 | 0.9722 | 0.9718 | 0.9892 | 0.9515 | 0.9479 | 0.9159 | 0.982 | 0.9464 | 0.93 | 0.6896 | 0.9492 |
Cytoplasmic Foci | 0.0285 | 0.0033 | 0.0094 | 0.0106 | 0.0096 | 0.0103 | 0.0044 | 0.0011 | 0.0049 | 0.0011 | 0.0038 | 0.003 | 0.0088 | 0.0011 | 0.0041 | 0.0043 | 0.0338 | 0.0054 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0254 | 0.0049 | 0.007 | 0.0253 | 0.015 | 0.0199 | 0.0336 | 0.0226 | 0.0108 | 0.0034 | 0.018 | 0.0341 | 0.019 | 0.0079 | 0.0103 | 0.0106 | 0.0072 | 0.03 |
Endosome | 0.0493 | 0.006 | 0.0227 | 0.0234 | 0.0459 | 0.0134 | 0.0056 | 0.0008 | 0.0028 | 0.0014 | 0.0059 | 0.0047 | 0.0176 | 0.0015 | 0.0089 | 0.0105 | 0.0995 | 0.0063 |
Golgi | 0.0117 | 0.0013 | 0.006 | 0.0105 | 0.0224 | 0.0046 | 0.0012 | 0.0001 | 0.0007 | 0 | 0.001 | 0.0016 | 0.0035 | 0.0015 | 0.0019 | 0.0089 | 0.0178 | 0.0006 |
Lipid Particles | 0.008 | 0.0003 | 0.0037 | 0.0019 | 0.0059 | 0.0006 | 0.0013 | 0 | 0.0016 | 0 | 0.0005 | 0.0005 | 0.0035 | 0 | 0.0027 | 0.0012 | 0.0285 | 0.0004 |
Mitochondria | 0.0139 | 0.0001 | 0.0171 | 0.0046 | 0.0915 | 0.0073 | 0.0024 | 0 | 0.001 | 0 | 0.0038 | 0.0007 | 0.0051 | 0.0005 | 0.0027 | 0.021 | 0.0321 | 0.0011 |
None | 0.0019 | 0.0002 | 0.0015 | 0.0004 | 0.0009 | 0.0005 | 0.0027 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0004 | 0.0017 | 0.0001 | 0.0005 | 0.0003 | 0.0012 | 0.0003 |
Nuclear Periphery | 0.005 | 0.0006 | 0.0009 | 0.0022 | 0.0238 | 0.0013 | 0.005 | 0.0006 | 0.0007 | 0.0007 | 0.0011 | 0.0013 | 0.0043 | 0.0006 | 0.002 | 0.0043 | 0.002 | 0.0016 |
Nucleolus | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0008 | 0 |
Nucleus | 0.0017 | 0.0012 | 0.0009 | 0.0011 | 0.0075 | 0.0017 | 0.003 | 0.0007 | 0.0007 | 0.001 | 0.001 | 0.0013 | 0.0019 | 0.0004 | 0.001 | 0.0011 | 0.0025 | 0.0009 |
Peroxisomes | 0.0068 | 0 | 0.0016 | 0.0047 | 0.0013 | 0.0014 | 0.0005 | 0 | 0.0006 | 0 | 0 | 0 | 0.0029 | 0 | 0.0007 | 0.0002 | 0.0199 | 0.0001 |
Punctate Nuclear | 0.0029 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0002 | 0.0027 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0018 | 0 | 0.0006 | 0.0001 | 0.0005 | 0.0001 |
Vacuole | 0.007 | 0.0013 | 0.0035 | 0.0045 | 0.0097 | 0.0026 | 0.0041 | 0.0009 | 0.003 | 0.0021 | 0.006 | 0.0013 | 0.0037 | 0.0003 | 0.0027 | 0.0035 | 0.0293 | 0.0018 |
Vacuole Periphery | 0.0032 | 0.0001 | 0.0007 | 0.001 | 0.0081 | 0.0009 | 0.0007 | 0.0001 | 0.0002 | 0.0003 | 0.0015 | 0.0003 | 0.0012 | 0.0001 | 0.0009 | 0.0026 | 0.0126 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 288.3055 | 330.6871 | 337.491 | 387.478 | 362.1194 | 346.4107 | 362.3802 | 464.9163 | 461.4006 | 352.8765 |
Translational Efficiency | 2.1279 | 1.9362 | 1.7377 | 1.6795 | 1.5493 | 1.7593 | 1.8307 | 1.5728 | 1.6428 | 1.7209 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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