Standard name | |
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Human Ortholog | |
Description | Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 32 | 20 | 35 | 17 | 8 | 14 | 8 | 7 | – | 8 | 2 | 5 | 40 | 110 | 79 | 14 | 11 | 12 | 17 | 11 | 12 | 19 | 42 | 24 |
Bud | 3 | 7 | 7 | 3 | 3 | 5 | 10 | 14 | – | 12 | 5 | 8 | 23 | 27 | 11 | 0 | 2 | 1 | 11 | 12 | 8 | 34 | 39 | 28 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 4 | 9 | 12 | 7 | 10 |
Bud Site | 1 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | – | 1 | 0 | 1 | 0 | 2 | 2 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 6 | 10 | 0 | 0 | 1 | 0 | 6 | – | 1 | 2 | 5 | 28 | 56 | 89 | 4 | 5 | 1 | 1 | 0 | 2 | 1 | 0 | 0 |
Cytoplasm | 28 | 25 | 59 | 140 | 169 | 323 | 355 | 423 | – | 415 | 355 | 458 | 62 | 76 | 77 | 55 | 36 | 40 | 6 | 2 | 3 | 2 | 2 | 1 |
Endoplasmic Reticulum | 92 | 43 | 84 | 47 | 62 | 73 | 53 | 16 | – | 32 | 6 | 10 | 55 | 81 | 59 | 4 | 8 | 7 | 73 | 30 | 67 | 12 | 25 | 27 |
Endosome | 1 | 0 | 3 | 7 | 4 | 2 | 3 | 16 | – | 0 | 2 | 6 | 0 | 4 | 1 | 4 | 2 | 1 | 3 | 7 | 8 | 6 | 15 | 9 |
Golgi | 19 | 2 | 4 | 2 | 4 | 1 | 0 | 0 | – | 1 | 0 | 0 | 2 | 6 | 8 | 6 | 6 | 6 | 11 | 3 | 4 | 2 | 15 | 8 |
Mitochondria | 3 | 2 | 10 | 3 | 9 | 25 | 27 | 22 | – | 17 | 28 | 33 | 0 | 0 | 2 | 10 | 9 | 5 | 3 | 2 | 3 | 4 | 7 | 5 |
Nucleus | 3 | 2 | 1 | 8 | 38 | 78 | 61 | 62 | – | 65 | 41 | 45 | 2 | 4 | 2 | 37 | 23 | 19 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 2 |
Nucleolus | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 8 | – | 3 | 2 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 6 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 2 |
SpindlePole | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | – | 0 | 0 | 0 | 1 | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 |
Vac/Vac Membrane | 130 | 42 | 100 | 150 | 290 | 326 | 448 | 480 | – | 313 | 247 | 265 | 14 | 14 | 8 | 164 | 133 | 111 | 190 | 75 | 199 | 70 | 146 | 154 |
Unique Cell Count | 270 | 125 | 263 | 324 | 486 | 675 | 822 | 918 | 742 | 588 | 703 | 174 | 292 | 264 | 260 | 207 | 181 | 330 | 154 | 323 | 176 | 320 | 283 | |
Labelled Cell Count | 323 | 150 | 315 | 379 | 590 | 852 | 966 | 1056 | 870 | 692 | 838 | 227 | 390 | 345 | 302 | 237 | 203 | 330 | 154 | 323 | 176 | 320 | 283 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.3 | 15.1 | 10.7 | 10.5 | 10.9 | 9.4 | 9.0 | 9.1 | – | 9.3 | 8.5 | 8.6 | 12.5 | 11.6 | 11.7 | 12.2 | 11.5 | 11.9 | 14.3 | 14.3 | 13.9 |
Std Deviation (1e-4) | 3.2 | 3.1 | 2.0 | 2.0 | 2.7 | 2.4 | 2.5 | 2.2 | – | 2.4 | 2.2 | 2.3 | 2.1 | 2.4 | 2.4 | 2.7 | 2.7 | 2.6 | 2.3 | 3.3 | 2.5 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.04 | -0.18 | -0.24 | -0.22 | – | -0.2 | -0.34 | -0.31 | 0.23 | 0.12 | 0.13 | 0.2 | 0.11 | 0.15 | 0.42 | 0.43 | 0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -3.4 | -6.5 | -6.9 | -8.9 | -9.7 | – | -8.4 | -8.6 | -8.7 | 2.6 | 6.5 | 4.6 | -3.1 | -2.9 | -2.2 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 4.3 | 3.2 | 1.0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 4.5 | 5.6 | 8.8 | -1.6 | -0.8 | 0 |
Cytoplasm | 5.3 | 3.5 | 7.1 | 6.0 | 6.9 | – | 9.4 | 10.2 | 11.8 | 3.0 | 1.0 | 1.8 | -0.4 | -1.4 | -0.1 |
Endoplasmic Reticulum | -5.0 | -6.3 | -7.8 | -10.8 | -15.5 | – | -12.0 | -13.6 | -14.2 | -0.1 | -1.1 | -2.5 | -9.3 | -7.6 | -7.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.2 | 0 | 0 | 0 |
Mitochondria | -2.4 | -1.6 | -0.1 | -0.4 | -1.2 | – | -1.3 | 0.6 | 0.6 | 0 | 0 | -2.3 | 0 | 0.3 | -0.6 |
Nucleus | 0 | 4.4 | 5.5 | 4.3 | 4.1 | – | 4.7 | 4.1 | 3.9 | 0 | 0 | 0 | 6.1 | 5.2 | 5.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.0 | 5.7 | 2.8 | 4.7 | 4.1 | – | 1.2 | 1.1 | -0.1 | -7.0 | -9.7 | -10.0 | 5.7 | 5.6 | 4.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.5979 | 8.426 | 8.2535 | 7.4746 | 7.2038 | 8.1775 | 11.3323 | 12.1846 | 11.949 | 11.3195 | 11.5687 | 12.2654 | 8.7183 | 9.0675 | 9.1746 | 8.968 | 8.6319 | 9.2268 |
Actin | 0.0485 | 0.0127 | 0.0285 | 0.0025 | 0.0123 | 0.014 | 0.0244 | 0.0066 | 0.0134 | 0.0099 | 0.004 | 0.0088 | 0.0486 | 0.0163 | 0.0295 | 0.0017 | 0.0096 | 0.0086 |
Bud | 0.0249 | 0.0144 | 0.1447 | 0.0379 | 0.0292 | 0.0042 | 0.0163 | 0.0239 | 0.0835 | 0.0198 | 0.015 | 0.0061 | 0.0248 | 0.0218 | 0.1445 | 0.0245 | 0.0274 | 0.0076 |
Bud Neck | 0.0302 | 0.023 | 0.0111 | 0.0037 | 0.0051 | 0.4193 | 0.0174 | 0.0097 | 0.0063 | 0.003 | 0.0027 | 0.3336 | 0.0279 | 0.014 | 0.0036 | 0.0048 | 0.0277 | 0.3727 |
Bud Periphery | 0.0597 | 0.0302 | 0.3292 | 0.2293 | 0.1058 | 0.0231 | 0.0708 | 0.0528 | 0.284 | 0.1483 | 0.1103 | 0.0275 | 0.0588 | 0.04 | 0.33 | 0.1832 | 0.1507 | 0.0246 |
Bud Site | 0.1192 | 0.2626 | 0.0391 | 0.0074 | 0.013 | 0.0065 | 0.0661 | 0.1808 | 0.0309 | 0.0132 | 0.0038 | 0.0116 | 0.126 | 0.2688 | 0.0564 | 0.0401 | 0.0182 | 0.0118 |
Cell Periphery | 0.1109 | 0.0433 | 0.0181 | 0.0371 | 0.0625 | 0.063 | 0.1684 | 0.0366 | 0.0377 | 0.0579 | 0.1034 | 0.0844 | 0.1368 | 0.0387 | 0.0104 | 0.0376 | 0.0348 | 0.0519 |
Cytoplasm | 0.0933 | 0.0733 | 0.033 | 0.0597 | 0.0441 | 0.0252 | 0.0778 | 0.0403 | 0.029 | 0.0386 | 0.0197 | 0.0415 | 0.119 | 0.0754 | 0.0262 | 0.0195 | 0.0394 | 0.0566 |
Cytoplasmic Foci | 0.0414 | 0.017 | 0.0064 | 0.0018 | 0.0317 | 0.0134 | 0.0114 | 0.0102 | 0.0062 | 0.004 | 0.006 | 0.0124 | 0.0271 | 0.0209 | 0.0218 | 0.0081 | 0.0139 | 0.0199 |
Eisosomes | 0.001 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0321 | 0.0262 | 0.009 | 0.0101 | 0.0122 | 0.0241 | 0.0561 | 0.0365 | 0.0142 | 0.0411 | 0.0164 | 0.0328 | 0.0284 | 0.0266 | 0.0091 | 0.0104 | 0.0485 | 0.0319 |
Endosome | 0.0697 | 0.091 | 0.0587 | 0.0407 | 0.0857 | 0.0676 | 0.0626 | 0.1216 | 0.0606 | 0.0721 | 0.0575 | 0.053 | 0.0649 | 0.0875 | 0.0789 | 0.0878 | 0.0924 | 0.0751 |
Golgi | 0.0341 | 0.0261 | 0.0125 | 0.0039 | 0.0302 | 0.0261 | 0.0242 | 0.0273 | 0.0146 | 0.0106 | 0.0095 | 0.0181 | 0.0162 | 0.0136 | 0.0291 | 0.0074 | 0.025 | 0.0328 |
Lipid Particles | 0.0577 | 0.0157 | 0.002 | 0.0028 | 0.0503 | 0.0111 | 0.0468 | 0.0119 | 0.0028 | 0.0039 | 0.0059 | 0.0151 | 0.0235 | 0.0164 | 0.0089 | 0.0132 | 0.0127 | 0.0197 |
Mitochondria | 0.013 | 0.0044 | 0.0071 | 0.0037 | 0.0141 | 0.0147 | 0.0114 | 0.0052 | 0.0179 | 0.0124 | 0.0024 | 0.0049 | 0.0028 | 0.0029 | 0.0152 | 0.0031 | 0.038 | 0.0028 |
None | 0.004 | 0.0004 | 0.0004 | 0.0003 | 0.0035 | 0.0007 | 0.0017 | 0.0011 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0.0087 | 0.0003 | 0.0016 | 0.0002 | 0.005 | 0.003 |
Nuclear Periphery | 0.0083 | 0.0046 | 0.0037 | 0.0048 | 0.0031 | 0.0052 | 0.0048 | 0.0052 | 0.0037 | 0.0076 | 0.0055 | 0.0026 | 0.004 | 0.0029 | 0.0041 | 0.0047 | 0.0258 | 0.0044 |
Nucleolus | 0.002 | 0.0022 | 0.0013 | 0.0017 | 0.0048 | 0.002 | 0.0016 | 0.0042 | 0.0013 | 0.0007 | 0.0009 | 0.002 | 0.0015 | 0.0017 | 0.0008 | 0.0012 | 0.0027 | 0.0018 |
Nucleus | 0.0037 | 0.0043 | 0.0034 | 0.0039 | 0.0057 | 0.0041 | 0.0034 | 0.0106 | 0.0038 | 0.0029 | 0.003 | 0.0033 | 0.0029 | 0.0034 | 0.0022 | 0.0026 | 0.008 | 0.0037 |
Peroxisomes | 0.0155 | 0.0006 | 0.0005 | 0.0002 | 0.0039 | 0.0012 | 0.0021 | 0.0005 | 0.0003 | 0.0005 | 0.0002 | 0.0006 | 0.0028 | 0.0006 | 0.0039 | 0.0003 | 0.0014 | 0.001 |
Punctate Nuclear | 0.0029 | 0.0004 | 0.0002 | 0.0001 | 0.0007 | 0.0005 | 0.0005 | 0.002 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0045 | 0.0003 | 0.0054 | 0.0001 | 0.002 | 0.0015 |
Vacuole | 0.2184 | 0.3251 | 0.2483 | 0.4836 | 0.4454 | 0.2607 | 0.3142 | 0.3818 | 0.3429 | 0.4881 | 0.5707 | 0.3254 | 0.2625 | 0.3292 | 0.1818 | 0.4885 | 0.3805 | 0.2586 |
Vacuole Periphery | 0.0096 | 0.0222 | 0.0427 | 0.0649 | 0.0366 | 0.013 | 0.0172 | 0.0313 | 0.0463 | 0.0643 | 0.0626 | 0.0156 | 0.008 | 0.0183 | 0.0363 | 0.0609 | 0.036 | 0.0101 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 56.7715 | 30.3634 | 24.4622 | 37.8826 | 31.4559 | 54.3153 | 42.7613 | 43.2174 | 38.9377 | 53.0903 |
Translational Efficiency | 1.0314 | 1.2147 | 1.3817 | 0.6952 | 1.1887 | 1.1075 | 0.9016 | 0.8251 | 0.9499 | 0.8888 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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