Standard name
Human Ortholog
Description Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.12 0.16 0.13 0.05 0 0 0 0 0 0 0 0.23 0.38 0.3 0.05 0.05 0.07 0.05 0.07 0 0.11 0.13 0.09
Bud 0 0.06 0 0 0 0 0 0 0 0 0 0.13 0.09 0 0 0 0 0 0.08 0 0.19 0.12 0.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0.16 0.19 0.34 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.2 0.22 0.43 0.35 0.48 0.43 0.46 0.56 0.6 0.65 0.36 0.26 0.29 0.21 0.17 0.22 0 0 0 0 0 0
Endoplasmic Reticulum 0.34 0.34 0.32 0.15 0.13 0.11 0.06 0 0 0 0 0.32 0.28 0.22 0 0 0 0.22 0.2 0.21 0.07 0.08 0.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0
Golgi 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0.08 0.12 0.07 0.07 0.09 0.07 0.06 0 0 0 0.14 0.11 0.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.48 0.34 0.38 0.46 0.6 0.48 0.55 0.52 0.42 0.42 0.38 0.08 0.05 0 0.63 0.64 0.61 0.58 0.49 0.62 0.4 0.46 0.55
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 32 20 35 17 8 14 8 7 8 2 5 40 110 79 14 11 12 17 11 12 19 42 24
Bud 3 7 7 3 3 5 10 14 12 5 8 23 27 11 0 2 1 11 12 8 34 39 28
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 4 9 12 7 10
Bud Site 1 0 2 0 0 2 0 0 1 0 1 0 2 2 0 1 0
Cell Periphery 8 6 10 0 0 1 0 6 1 2 5 28 56 89 4 5 1 1 0 2 1 0 0
Cytoplasm 28 25 59 140 169 323 355 423 415 355 458 62 76 77 55 36 40 6 2 3 2 2 1
Endoplasmic Reticulum 92 43 84 47 62 73 53 16 32 6 10 55 81 59 4 8 7 73 30 67 12 25 27
Endosome 1 0 3 7 4 2 3 16 0 2 6 0 4 1 4 2 1 3 7 8 6 15 9
Golgi 19 2 4 2 4 1 0 0 1 0 0 2 6 8 6 6 6 11 3 4 2 15 8
Mitochondria 3 2 10 3 9 25 27 22 17 28 33 0 0 2 10 9 5 3 2 3 4 7 5
Nucleus 3 2 1 8 38 78 61 62 65 41 45 2 4 2 37 23 19 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 0 1 2 1 0 1 0 0 0 0 0 0 1 1 3 2
Nucleolus 2 1 0 0 0 0 1 8 3 2 1 0 0 0 2 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 3 1 0 0 1 0 0 0 6 4 0 0 0 0 0 0 3 5 2
SpindlePole 1 0 0 1 0 1 0 2 0 0 0 1 3 3 2 0 0 0 0 0 2 2 1
Vac/Vac Membrane 130 42 100 150 290 326 448 480 313 247 265 14 14 8 164 133 111 190 75 199 70 146 154
Unique Cell Count 270 125 263 324 486 675 822 918 742 588 703 174 292 264 260 207 181 330 154 323 176 320 283
Labelled Cell Count 323 150 315 379 590 852 966 1056 870 692 838 227 390 345 302 237 203 330 154 323 176 320 283


Ambiguous, Bud, Bud Neck, Cytoplasm, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.3 15.1 10.7 10.5 10.9 9.4 9.0 9.1 9.3 8.5 8.6 12.5 11.6 11.7 12.2 11.5 11.9 14.3 14.3 13.9
Std Deviation (1e-4) 3.2 3.1 2.0 2.0 2.7 2.4 2.5 2.2 2.4 2.2 2.3 2.1 2.4 2.4 2.7 2.7 2.6 2.3 3.3 2.5
Intensity Change (Log2) -0.02 0.04 -0.18 -0.24 -0.22 -0.2 -0.34 -0.31 0.23 0.12 0.13 0.2 0.11 0.15 0.42 0.43 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -3.4 -6.5 -6.9 -8.9 -9.7 -8.4 -8.6 -8.7 2.6 6.5 4.6 -3.1 -2.9 -2.2
Bud 0 0 0 0 0 0 0 0 4.3 3.2 1.0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 4.5 5.6 8.8 -1.6 -0.8 0
Cytoplasm 5.3 3.5 7.1 6.0 6.9 9.4 10.2 11.8 3.0 1.0 1.8 -0.4 -1.4 -0.1
Endoplasmic Reticulum -5.0 -6.3 -7.8 -10.8 -15.5 -12.0 -13.6 -14.2 -0.1 -1.1 -2.5 -9.3 -7.6 -7.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 1.2 0 0 0
Mitochondria -2.4 -1.6 -0.1 -0.4 -1.2 -1.3 0.6 0.6 0 0 -2.3 0 0.3 -0.6
Nucleus 0 4.4 5.5 4.3 4.1 4.7 4.1 3.9 0 0 0 6.1 5.2 5.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.0 5.7 2.8 4.7 4.1 1.2 1.1 -0.1 -7.0 -9.7 -10.0 5.7 5.6 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.5979 8.426 8.2535 7.4746 7.2038 8.1775 11.3323 12.1846 11.949 11.3195 11.5687 12.2654 8.7183 9.0675 9.1746 8.968 8.6319 9.2268
Actin 0.0485 0.0127 0.0285 0.0025 0.0123 0.014 0.0244 0.0066 0.0134 0.0099 0.004 0.0088 0.0486 0.0163 0.0295 0.0017 0.0096 0.0086
Bud 0.0249 0.0144 0.1447 0.0379 0.0292 0.0042 0.0163 0.0239 0.0835 0.0198 0.015 0.0061 0.0248 0.0218 0.1445 0.0245 0.0274 0.0076
Bud Neck 0.0302 0.023 0.0111 0.0037 0.0051 0.4193 0.0174 0.0097 0.0063 0.003 0.0027 0.3336 0.0279 0.014 0.0036 0.0048 0.0277 0.3727
Bud Periphery 0.0597 0.0302 0.3292 0.2293 0.1058 0.0231 0.0708 0.0528 0.284 0.1483 0.1103 0.0275 0.0588 0.04 0.33 0.1832 0.1507 0.0246
Bud Site 0.1192 0.2626 0.0391 0.0074 0.013 0.0065 0.0661 0.1808 0.0309 0.0132 0.0038 0.0116 0.126 0.2688 0.0564 0.0401 0.0182 0.0118
Cell Periphery 0.1109 0.0433 0.0181 0.0371 0.0625 0.063 0.1684 0.0366 0.0377 0.0579 0.1034 0.0844 0.1368 0.0387 0.0104 0.0376 0.0348 0.0519
Cytoplasm 0.0933 0.0733 0.033 0.0597 0.0441 0.0252 0.0778 0.0403 0.029 0.0386 0.0197 0.0415 0.119 0.0754 0.0262 0.0195 0.0394 0.0566
Cytoplasmic Foci 0.0414 0.017 0.0064 0.0018 0.0317 0.0134 0.0114 0.0102 0.0062 0.004 0.006 0.0124 0.0271 0.0209 0.0218 0.0081 0.0139 0.0199
Eisosomes 0.001 0.0002 0.0002 0 0.0001 0.0002 0.0007 0.0001 0.0002 0.0005 0.0001 0.0002 0.0005 0.0001 0.0002 0 0.0003 0.0001
Endoplasmic Reticulum 0.0321 0.0262 0.009 0.0101 0.0122 0.0241 0.0561 0.0365 0.0142 0.0411 0.0164 0.0328 0.0284 0.0266 0.0091 0.0104 0.0485 0.0319
Endosome 0.0697 0.091 0.0587 0.0407 0.0857 0.0676 0.0626 0.1216 0.0606 0.0721 0.0575 0.053 0.0649 0.0875 0.0789 0.0878 0.0924 0.0751
Golgi 0.0341 0.0261 0.0125 0.0039 0.0302 0.0261 0.0242 0.0273 0.0146 0.0106 0.0095 0.0181 0.0162 0.0136 0.0291 0.0074 0.025 0.0328
Lipid Particles 0.0577 0.0157 0.002 0.0028 0.0503 0.0111 0.0468 0.0119 0.0028 0.0039 0.0059 0.0151 0.0235 0.0164 0.0089 0.0132 0.0127 0.0197
Mitochondria 0.013 0.0044 0.0071 0.0037 0.0141 0.0147 0.0114 0.0052 0.0179 0.0124 0.0024 0.0049 0.0028 0.0029 0.0152 0.0031 0.038 0.0028
None 0.004 0.0004 0.0004 0.0003 0.0035 0.0007 0.0017 0.0011 0.0003 0.0005 0.0003 0.0002 0.0087 0.0003 0.0016 0.0002 0.005 0.003
Nuclear Periphery 0.0083 0.0046 0.0037 0.0048 0.0031 0.0052 0.0048 0.0052 0.0037 0.0076 0.0055 0.0026 0.004 0.0029 0.0041 0.0047 0.0258 0.0044
Nucleolus 0.002 0.0022 0.0013 0.0017 0.0048 0.002 0.0016 0.0042 0.0013 0.0007 0.0009 0.002 0.0015 0.0017 0.0008 0.0012 0.0027 0.0018
Nucleus 0.0037 0.0043 0.0034 0.0039 0.0057 0.0041 0.0034 0.0106 0.0038 0.0029 0.003 0.0033 0.0029 0.0034 0.0022 0.0026 0.008 0.0037
Peroxisomes 0.0155 0.0006 0.0005 0.0002 0.0039 0.0012 0.0021 0.0005 0.0003 0.0005 0.0002 0.0006 0.0028 0.0006 0.0039 0.0003 0.0014 0.001
Punctate Nuclear 0.0029 0.0004 0.0002 0.0001 0.0007 0.0005 0.0005 0.002 0.0001 0.0001 0.0002 0.0003 0.0045 0.0003 0.0054 0.0001 0.002 0.0015
Vacuole 0.2184 0.3251 0.2483 0.4836 0.4454 0.2607 0.3142 0.3818 0.3429 0.4881 0.5707 0.3254 0.2625 0.3292 0.1818 0.4885 0.3805 0.2586
Vacuole Periphery 0.0096 0.0222 0.0427 0.0649 0.0366 0.013 0.0172 0.0313 0.0463 0.0643 0.0626 0.0156 0.008 0.0183 0.0363 0.0609 0.036 0.0101

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 56.7715 30.3634 24.4622 37.8826 31.4559 54.3153 42.7613 43.2174 38.9377 53.0903
Translational Efficiency 1.0314 1.2147 1.3817 0.6952 1.1887 1.1075 0.9016 0.8251 0.9499 0.8888

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
Localization
Cell Percentages vacuole (28%), ER (7%), mixed (46%)
Cell Cycle Regulation Yes
vacuole - S/G2 (5.5e-05)
ER - T/G1 (2.1e-05)
Subcompartmental Group N/A

Slg1

Slg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Slg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available