Standard name
Human Ortholog
Description Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.08 0.05 0 0 0.05 0.07 0.1 0.08 0.06 0.09 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.91 0.93 0.91 0.8 0.68 0.7 0.63 0.57 0.54 0.54 0.98 0.98 0.99 0.93 0.96 0.95 0.93 0.93 0.91 0.94 0.92 0.93
Endoplasmic Reticulum 0 0 0.05 0 0 0 0.1 0.13 0.12 0.17 0.09 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.1 0.06 0.21 0.28 0.17 0.22 0.3 0.34 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.05 0.05 0.09 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 2 0 4 11 6 9 14 11 13 0 0 0 0 1 1 0 0 1 0 3 1
Bud 0 0 0 0 0 0 1 2 7 5 1 5 0 0 0 0 0 1 2 0 1 0 5 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 2 19 9 5 8 14 20 29 23 12 26 9 12 10 9 17 20 0 0 3 0 0 0
Cytoplasm 169 143 221 172 197 169 175 198 180 170 114 162 299 262 343 211 313 314 164 128 229 156 287 280
Endoplasmic Reticulum 1 1 13 1 7 7 27 36 34 49 19 36 3 5 2 9 12 9 1 2 5 1 5 2
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 1 2 0 0
Golgi 0 0 0 0 1 1 1 4 1 0 0 2 0 0 0 1 2 0 2 0 0 0 0 0
Mitochondria 7 1 12 19 13 44 73 49 64 89 72 93 0 0 0 4 5 3 1 1 2 0 0 0
Nucleus 0 0 0 1 1 0 0 0 1 4 1 1 2 1 2 2 2 2 0 0 1 1 1 1
Nuclear Periphery 0 0 0 1 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 5 3 3 0 2 12 15 15 19 21 1 2 3 8 7 6 1 1 1 0 1 3
Unique Cell Count 174 146 244 185 217 210 259 282 287 296 212 298 304 268 347 227 326 331 176 138 251 167 311 300
Labelled Cell Count 182 148 270 208 227 233 304 327 340 369 250 361 314 283 360 244 359 356 176 138 251 167 311 300


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.3 15.7 13.5 12.4 14.0 13.8 13.5 14.3 14.0 13.3 13.0 13.6 14.5 14.5 14.4 20.8 22.1 22.7 13.5 16.4 19.6
Std Deviation (1e-4) 2.5 3.2 3.4 2.9 3.1 3.2 3.5 3.6 3.6 3.7 3.8 4.3 3.2 2.9 3.0 6.6 6.4 6.7 3.3 4.6 6.0
Intensity Change (Log2) – – – -0.12 0.04 0.02 0.0 0.08 0.05 -0.03 -0.06 0.01 0.1 0.1 0.09 0.62 0.71 0.75 -0.01 0.28 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.3 0 0 3.4 3.6 3.3 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.2 -2.6 -1.8 -1.1 -0.3 0.9 0 -0.9 0.4 -2.6 -1.6 -2.7 -1.8 -1.2 -0.8
Cytoplasm 0.9 0.1 -3.1 -6.3 -5.8 -7.4 -8.6 -8.9 -9.2 4.1 3.5 4.7 0.9 2.6 2.0
Endoplasmic Reticulum -2.8 -1.1 -1.0 2.1 2.9 2.6 4.1 1.5 2.7 -3.0 -2.1 -3.6 -0.7 -1.0 -1.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.1 0.5 5.2 7.0 4.5 5.7 7.5 8.0 7.7 -3.9 -3.7 0 -1.9 -2.4 -3.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 1.4 1.9 1.8 3.3 2.7 0 0 0 1.0 0.1 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.6529 11.2739 10.6754 8.9884 9.396 9.885 8.1045 10.6985 10.0061 8.8633 8.996 9.6 7.7736 11.9207 10.8444 7.8578 11.2836 9.9689
Actin 0.004 0.001 0.0076 0.0096 0.0008 0.0006 0.0087 0.0002 0.0046 0.0006 0.0007 0.0043 0.0269 0.0003 0.0037 0.0023 0.0013 0.0016
Bud 0.0009 0.0005 0.0013 0.001 0.0003 0.0004 0.0005 0.0003 0.0005 0.0004 0.0002 0.0003 0.0012 0.0002 0.0004 0.0005 0.0004 0.0011
Bud Neck 0.0032 0.0003 0.0005 0.0008 0.0005 0.0012 0.0011 0.0003 0.0003 0.0009 0.0004 0.0009 0.0019 0.0001 0.0019 0.0013 0.0004 0.0036
Bud Periphery 0.001 0.0001 0.001 0.0007 0.0001 0.0003 0.0005 0.0002 0.0003 0.0004 0.0001 0.0002 0.001 0.0001 0.0003 0.0003 0.0001 0.0004
Bud Site 0.0024 0.0018 0.0011 0.0047 0.0002 0.0002 0.0044 0.001 0.0005 0.0004 0.0001 0.0002 0.0031 0.0002 0.0025 0.0003 0.0005 0.0002
Cell Periphery 0.0009 0.0004 0.0004 0.0003 0.0001 0.0003 0.0007 0.0001 0.0002 0.0001 0 0.0001 0.0004 0.0001 0.0004 0.0006 0.0001 0.0001
Cytoplasm 0.848 0.968 0.9268 0.8141 0.9516 0.9278 0.8181 0.9746 0.924 0.8539 0.8324 0.9307 0.8075 0.9596 0.9231 0.8529 0.9588 0.9273
Cytoplasmic Foci 0.0154 0.0058 0.0084 0.0261 0.0119 0.0085 0.029 0.0038 0.0092 0.0194 0.0148 0.0065 0.0124 0.0079 0.006 0.0094 0.0109 0.0093
Eisosomes 0.0001 0 0.0002 0 0 0 0.0001 0 0.0001 0 0 0 0.0002 0 0.0003 0.0001 0 0
Endoplasmic Reticulum 0.0523 0.0045 0.0156 0.0353 0.0078 0.0284 0.0237 0.0032 0.0135 0.012 0.0062 0.0104 0.066 0.0086 0.0267 0.0207 0.0055 0.0172
Endosome 0.0251 0.0086 0.0149 0.0405 0.0171 0.0198 0.0467 0.0061 0.0153 0.0647 0.0653 0.0174 0.0324 0.0168 0.0174 0.0418 0.01 0.0241
Golgi 0.0039 0.0011 0.0019 0.0106 0.0017 0.0025 0.0079 0.0004 0.0046 0.0127 0.0129 0.0065 0.0067 0.0008 0.0008 0.0018 0.0011 0.0035
Lipid Particles 0.0014 0.0001 0.0005 0.0044 0.0001 0.0002 0.0131 0.0001 0.0022 0.001 0.0374 0.0037 0.0093 0.0001 0.0008 0.0009 0.0003 0.0001
Mitochondria 0.0029 0.0003 0.0009 0.0056 0.0003 0.0034 0.0028 0.0002 0.0088 0.0056 0.0105 0.0033 0.0025 0.0003 0.0016 0.0024 0.0016 0.0056
None 0.0102 0.0014 0.0062 0.0008 0.0019 0.001 0.0034 0.0009 0.0016 0.0009 0.0011 0.0006 0.0049 0.0007 0.0027 0.0027 0.0026 0.0007
Nuclear Periphery 0.0059 0.0004 0.0012 0.0021 0.0007 0.001 0.0047 0.0007 0.0012 0.0036 0.0018 0.004 0.0061 0.0008 0.0021 0.0237 0.0006 0.0009
Nucleolus 0.0002 0 0 0.0002 0 0 0.0009 0.0001 0 0 0.0001 0.0001 0.0003 0 0.0002 0.0016 0 0
Nucleus 0.0054 0.0009 0.0009 0.0013 0.0008 0.0008 0.0044 0.0017 0.0011 0.0017 0.0015 0.0019 0.0019 0.0007 0.0011 0.0143 0.0008 0.0009
Peroxisomes 0.0007 0 0.0026 0.0224 0.0001 0.0001 0.008 0 0.001 0.001 0.0026 0.0008 0.0049 0 0.0002 0.0002 0.0001 0.0002
Punctate Nuclear 0.0022 0.0001 0.0001 0.0026 0.0001 0.0004 0.0077 0.0001 0.0012 0.0002 0.0003 0.0033 0.0032 0.0001 0.0003 0.0042 0.0005 0.0001
Vacuole 0.0122 0.0042 0.007 0.0143 0.0033 0.0025 0.0119 0.0058 0.0087 0.0168 0.0092 0.0037 0.0059 0.0023 0.0065 0.0151 0.0033 0.0024
Vacuole Periphery 0.0015 0.0004 0.0009 0.0026 0.0006 0.0007 0.0015 0.0005 0.0009 0.0036 0.0024 0.0009 0.0012 0.0004 0.0011 0.0031 0.001 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.3364 41.8329 46.2904 38.8337 13.8378 12.308 66.2285 73.1224 57.1232 23.3079
Translational Efficiency 0.8972 0.7459 0.6472 0.6871 0.5961 0.3373 0.67 0.6073 0.57 0.4508

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Hst3

Hst3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hst3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available