Standard name
Human Ortholog
Description Hsp90 cochaperone; regulates spatial organization of amyloid-like proteins in the cytosol, thereby buffering the proteotoxicity caused by amyloid-like proteins; interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.06 0.08 0.07 0.09 0.05 0.08 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Cytoplasm 1.0 0.98 0.98 1.0 0.95 0.94 0.85 0.81 0.79 0.79 0.81 0.83 1.0 1.0 0.97 0.99 0.99 0.99 0.99 0.95 0.91 0.94 1.0 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0.12 0.14 0.15 0.12 0.15 0.13 0 0 0 0 0.09 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 2 0 4 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 1 0 1 2 0 1 0 3 0 0 0 0 0 0 0 2 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 7 4 3 7 7 17 25 14 22 6 15 0 0 0 14 14 10 0 0 0 0 0 0
Cytoplasm 184 309 98 181 225 187 241 258 149 198 93 151 16 36 30 288 245 278 180 304 89 6 14 61
Endoplasmic Reticulum 0 3 0 0 10 8 33 44 28 31 17 24 0 0 1 8 21 14 0 2 2 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 2 3 2 2 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 1 0 1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 1 1 1 2 0 0 0 0 0 1 1 0 0 4 1 0 0 0
Unique Cell Count 184 314 100 181 238 198 282 319 189 250 115 183 16 36 31 292 247 280 183 319 99 7 15 64
Labelled Cell Count 185 320 102 185 243 202 295 333 196 256 122 196 16 37 31 312 281 304 183 319 99 7 15 64


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 91.3 113.3 105.3 97.6 93.1 89.5 84.8 89.1 79.9 82.1 69.5 73.4 162.2 146.4 145.3 173.6 177.3 159.8 – – 149.7
Std Deviation (1e-4) 15.1 29.3 24.1 22.3 22.5 24.9 26.3 25.4 29.6 28.0 27.9 24.3 37.1 48.6 41.4 72.6 75.5 66.8 – – 43.6
Intensity Change (Log2) – – – -0.11 -0.18 -0.24 -0.31 -0.24 -0.4 -0.36 -0.6 -0.52 0.62 0.47 0.46 0.72 0.75 0.6 – – 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.8 1.3 1.1 1.5 0 1.3 0 0 0 0 0.6 0
Cytoplasm 1.9 -1.4 -1.4 -3.4 -4.2 -4.4 -4.4 -4.0 -3.8 0.6 0.9 -0.4 0.4 0.9 1.1
Endoplasmic Reticulum 0 0 0 3.6 3.9 4.1 3.7 4.0 3.8 0 0 0 0 3.0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 68.9322 99.0109 86.8093 90.536 74.5962 88.9856 68.6892 101.115 89.5774 70.6537 75.9139 90.1067 50.3815 84.4747 75.5309 75.6799 72.7706 80.9573
Actin 0.0011 0.0001 0.0015 0 0.0003 0.0001 0.005 0.0003 0.0064 0.0034 0.0003 0.0012 0.0456 0.0003 0.0143 0.0001 0.061 0.0087
Bud 0.0009 0.0001 0.0005 0.0001 0.0004 0.0001 0.001 0 0.0018 0.0006 0.0002 0.0007 0.0055 0.0003 0.0004 0.0001 0.0009 0.0002
Bud Neck 0.0003 0.0004 0.001 0.0005 0.0006 0.0013 0.0009 0.0001 0.0005 0.0254 0.0003 0.0013 0.0033 0.0002 0.0005 0.0001 0.0007 0.0006
Bud Periphery 0.0007 0 0.0004 0 0.0003 0 0.0013 0 0.0015 0.0006 0.0001 0.0004 0.0035 0.0001 0.0002 0 0.002 0.0002
Bud Site 0.0006 0.0001 0.0024 0 0.0002 0 0.0019 0 0.0036 0.0017 0.0003 0.0001 0.0044 0.0002 0.0023 0.0001 0.0016 0.0001
Cell Periphery 0.004 0.0022 0.0038 0.0009 0.0019 0.0003 0.0037 0.0004 0.0008 0.0014 0.0008 0.0021 0.0011 0.0017 0.0002 0 0.0001 0.0001
Cytoplasm 0.879 0.9797 0.9253 0.9612 0.8577 0.9676 0.8396 0.995 0.9364 0.8229 0.8473 0.979 0.7587 0.9774 0.8914 0.9588 0.8532 0.9099
Cytoplasmic Foci 0.0121 0.0033 0.0054 0.0003 0.0225 0.0008 0.0256 0.0002 0.0055 0.0242 0.0267 0.0014 0.0377 0.0029 0.0371 0.0016 0.0065 0.0028
Eisosomes 0.0004 0 0.0004 0 0.0005 0 0.0003 0 0.0002 0.0007 0.0004 0 0.0003 0 0.0001 0 0.0003 0.0001
Endoplasmic Reticulum 0.0168 0.0053 0.0026 0.0031 0.0099 0.0169 0.0178 0.0015 0.0007 0.0011 0.0137 0.0027 0.0102 0.0004 0.0009 0.0032 0.0014 0.0009
Endosome 0.0027 0.0002 0.0027 0.0005 0.0036 0.0012 0.0168 0.0001 0.0018 0.0045 0.0015 0.0023 0.0189 0.0003 0.0059 0.0125 0.0153 0.0358
Golgi 0.0014 0.0001 0.0013 0 0.0004 0.0001 0.0028 0 0.0026 0.0022 0.0003 0.0005 0.0096 0 0.0049 0.0002 0.0111 0.0171
Lipid Particles 0.0042 0.0003 0.0036 0.0002 0.0044 0.0001 0.0084 0.0001 0.0027 0.0231 0.007 0.0002 0.0163 0.0003 0.0052 0.0002 0.0211 0.0041
Mitochondria 0.0123 0.0001 0.0086 0 0.0213 0.0001 0.0069 0 0.0122 0.0366 0.0047 0.0005 0.03 0.0004 0.0051 0.0001 0.0049 0.006
None 0.0034 0.0004 0.0025 0 0.0067 0.0001 0.0153 0.0001 0.0022 0.0068 0.0251 0.0002 0.0104 0.001 0.0031 0.0079 0.0024 0.0003
Nuclear Periphery 0.0053 0.0012 0.0028 0.0021 0.0026 0.0027 0.01 0.0006 0.0009 0.0019 0.0052 0.0013 0.0083 0.001 0.0018 0.006 0.0052 0.0043
Nucleolus 0.0133 0.0001 0.0063 0 0.0209 0 0.0035 0 0.0037 0.0145 0.0242 0.0001 0.0012 0.0025 0.0019 0.0001 0.0001 0
Nucleus 0.0073 0.0009 0.0038 0.0015 0.0079 0.0019 0.0076 0.0005 0.0021 0.006 0.0105 0.002 0.0063 0.0041 0.0024 0.0045 0.0014 0.0035
Peroxisomes 0.0035 0 0.0014 0 0.0005 0 0.0036 0 0.0016 0.0044 0.0007 0 0.006 0 0.0052 0 0.0058 0.0003
Punctate Nuclear 0.0006 0.0002 0.0007 0 0.015 0 0.0033 0 0.0006 0.0012 0.024 0.0001 0.0082 0.0019 0.0077 0.0007 0.003 0.0002
Vacuole 0.0256 0.0048 0.0185 0.0284 0.0156 0.006 0.0185 0.0008 0.0075 0.0104 0.0042 0.0034 0.0111 0.0034 0.0066 0.0029 0.0013 0.0029
Vacuole Periphery 0.0046 0.0002 0.0046 0.001 0.0066 0.0008 0.0064 0.0001 0.0047 0.0064 0.0025 0.0005 0.0033 0.0012 0.0028 0.0006 0.0008 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 181.0955 333.8075 324.6147 370.7057 267.1284 248.5958 401.606 476.893 453.6003 282.0439
Translational Efficiency 3.2301 3.0304 2.8116 2.9244 3.1937 2.5238 2.3695 2.428 2.3902 2.6422

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Hsp90 cochaperone; regulates spatial organization of amyloid-like proteins in the cytosol, thereby buffering the proteotoxicity caused by amyloid-like proteins; interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Sti1

Sti1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sti1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available