Standard name | |
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Human Ortholog | |
Description | Protein containing a UCS (UNC-45/CRO1/SHE4) domain; binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 2 | 5 | 5 |
Bud | 1 | 0 | 1 | 2 | 1 | 3 | 6 | 2 | 8 | 13 | 10 | 12 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 8 | 4 |
Bud Neck | 0 | 1 | 10 | 2 | 2 | 1 | 4 | 7 | 8 | 4 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 4 | 5 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 5 | 2 | 7 | 2 | 2 | 6 | 7 | 15 | 4 | 2 | 3 | 4 | 3 | 8 | 13 | 11 | 0 | 0 | 4 | 1 | 3 | 2 |
Cytoplasm | 136 | 239 | 288 | 181 | 349 | 428 | 598 | 596 | 447 | 450 | 300 | 339 | 241 | 335 | 463 | 176 | 126 | 90 | 135 | 223 | 246 | 173 | 265 | 323 |
Endoplasmic Reticulum | 0 | 5 | 0 | 0 | 1 | 1 | 0 | 3 | 1 | 0 | 2 | 1 | 1 | 1 | 0 | 6 | 8 | 1 | 1 | 0 | 1 | 6 | 13 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 3 | 1 | 0 | 2 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 1 | 0 | 1 | 0 | 1 |
Mitochondria | 2 | 9 | 0 | 10 | 2 | 33 | 61 | 35 | 167 | 155 | 147 | 95 | 0 | 4 | 2 | 1 | 0 | 1 | 1 | 6 | 20 | 17 | 36 | 19 |
Nucleus | 0 | 0 | 0 | 4 | 2 | 0 | 2 | 10 | 1 | 1 | 2 | 1 | 0 | 0 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 0 | 2 | 1 | 5 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 7 | 1 | 9 | 5 | 8 | 0 | 0 | 1 | 4 | 0 | 2 | 1 | 2 | 1 | 8 | 14 | 12 |
Unique Cell Count | 139 | 247 | 289 | 187 | 352 | 442 | 630 | 639 | 540 | 553 | 386 | 412 | 242 | 337 | 465 | 182 | 135 | 100 | 144 | 249 | 292 | 221 | 360 | 392 |
Labelled Cell Count | 141 | 258 | 306 | 203 | 365 | 470 | 678 | 675 | 643 | 652 | 475 | 467 | 246 | 344 | 473 | 200 | 151 | 111 | 144 | 249 | 292 | 221 | 360 | 392 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 4.2 | 5.1 | 4.8 | 5.2 | 4.3 | 4.6 | 4.9 | 4.0 | 4.0 | 3.8 | 4.3 | 4.8 | 5.1 | 4.9 | 6.9 | 7.1 | 6.8 | 4.6 | 4.5 | 4.8 |
Std Deviation (1e-4) | 0.3 | 0.8 | 0.9 | 1.4 | 1.1 | 0.8 | 1.1 | 1.0 | 1.0 | 0.8 | 1.2 | 0.7 | 0.8 | 1.3 | 1.1 | 1.2 | 2.1 | 1.1 | 1.3 | 1.3 | 1.0 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.04 | -0.26 | -0.15 | -0.06 | -0.35 | -0.35 | -0.41 | -0.24 | -0.09 | -0.01 | -0.05 | 0.44 | 0.48 | 0.41 | -0.14 | -0.17 | -0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3685 | 3.1209 | 2.8869 | 2.6619 | 2.6198 | 2.8513 | 0.3025 | 0.7053 | 0.4227 | 0.3122 | 0.1248 | 0.3657 | -0.1339 | 1.0247 | 0.5342 | 0.5573 | 0.1308 | 0.1485 |
Actin | 0.0228 | 0.0008 | 0.0083 | 0.0001 | 0.0379 | 0.0031 | 0.04 | 0.002 | 0.014 | 0.0024 | 0.0141 | 0.0069 | 0.0258 | 0.001 | 0.0294 | 0.0686 | 0.0355 | 0.0125 |
Bud | 0.0008 | 0.0006 | 0.0008 | 0.0002 | 0.0025 | 0.0003 | 0.0013 | 0.0026 | 0.003 | 0.0002 | 0.0049 | 0.0021 | 0.0003 | 0.001 | 0.0022 | 0.0002 | 0.0002 | 0.0002 |
Bud Neck | 0.0056 | 0.0011 | 0.0008 | 0.0002 | 0.0098 | 0.0065 | 0.0041 | 0.0016 | 0.0012 | 0.0009 | 0.0056 | 0.0025 | 0.0113 | 0.0002 | 0.001 | 0.0002 | 0.0004 | 0.0006 |
Bud Periphery | 0.0016 | 0.0006 | 0.0011 | 0.0002 | 0.0052 | 0.0012 | 0.002 | 0.0032 | 0.0038 | 0.0003 | 0.0121 | 0.0018 | 0.0004 | 0.0006 | 0.002 | 0.0001 | 0.0002 | 0.0002 |
Bud Site | 0.0074 | 0.0114 | 0.0033 | 0.0003 | 0.0028 | 0.0001 | 0.0062 | 0.0108 | 0.0068 | 0.0002 | 0.002 | 0.0004 | 0.0008 | 0.0111 | 0.0044 | 0.0002 | 0.0002 | 0.0001 |
Cell Periphery | 0.0015 | 0.001 | 0.001 | 0.0001 | 0.0006 | 0.0003 | 0.0005 | 0.001 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0005 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.418 | 0.6113 | 0.5542 | 0.6719 | 0.4782 | 0.6234 | 0.5962 | 0.7572 | 0.7138 | 0.75 | 0.5715 | 0.8315 | 0.6416 | 0.7715 | 0.6737 | 0.7548 | 0.7497 | 0.8077 |
Cytoplasmic Foci | 0.0253 | 0.0092 | 0.0188 | 0.0069 | 0.011 | 0.0132 | 0.0453 | 0.0213 | 0.0307 | 0.0285 | 0.058 | 0.0169 | 0.026 | 0.0126 | 0.0264 | 0.0188 | 0.0131 | 0.0162 |
Eisosomes | 0.0013 | 0.0001 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0083 | 0.0025 | 0.0034 | 0.0011 | 0.003 | 0.0029 | 0.0074 | 0.0041 | 0.0045 | 0.0036 | 0.0031 | 0.0036 | 0.0107 | 0.004 | 0.0064 | 0.0162 | 0.0057 | 0.0037 |
Endosome | 0.0154 | 0.0049 | 0.0061 | 0.0042 | 0.0139 | 0.0113 | 0.0448 | 0.0178 | 0.026 | 0.0277 | 0.0444 | 0.0135 | 0.0255 | 0.0059 | 0.0213 | 0.0252 | 0.0158 | 0.0148 |
Golgi | 0.0045 | 0.0015 | 0.0023 | 0.0003 | 0.0079 | 0.0025 | 0.0102 | 0.0014 | 0.0084 | 0.0017 | 0.0161 | 0.0013 | 0.0065 | 0.0005 | 0.0048 | 0.0015 | 0.0073 | 0.0021 |
Lipid Particles | 0.0078 | 0.0002 | 0.0019 | 0.0001 | 0.0012 | 0.0014 | 0.0096 | 0.001 | 0.0036 | 0.0002 | 0.0037 | 0.0018 | 0.0159 | 0.0008 | 0.0027 | 0.0036 | 0.0492 | 0.0004 |
Mitochondria | 0.0024 | 0.0007 | 0.0102 | 0.0002 | 0.0252 | 0.0009 | 0.007 | 0.0006 | 0.0082 | 0.0005 | 0.0558 | 0.0007 | 0.0034 | 0.0001 | 0.0024 | 0.0003 | 0.0015 | 0.0015 |
None | 0.4408 | 0.3504 | 0.3702 | 0.3118 | 0.3923 | 0.3221 | 0.1946 | 0.1394 | 0.1589 | 0.1752 | 0.1789 | 0.107 | 0.211 | 0.1846 | 0.2078 | 0.1029 | 0.0921 | 0.1355 |
Nuclear Periphery | 0.0038 | 0.0003 | 0.0006 | 0.0003 | 0.001 | 0.0052 | 0.0057 | 0.0011 | 0.0018 | 0.0008 | 0.0017 | 0.0015 | 0.0042 | 0.0008 | 0.0017 | 0.0012 | 0.0066 | 0.0005 |
Nucleolus | 0.0012 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0068 | 0.0002 | 0.0001 | 0.0007 | 0.0005 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 |
Nucleus | 0.0069 | 0.0007 | 0.0012 | 0.0005 | 0.0008 | 0.0019 | 0.0023 | 0.0036 | 0.0017 | 0.0013 | 0.0015 | 0.002 | 0.0009 | 0.0009 | 0.0012 | 0.0006 | 0.0011 | 0.0007 |
Peroxisomes | 0.0097 | 0.0001 | 0.0117 | 0.0001 | 0.0018 | 0.0009 | 0.01 | 0.0003 | 0.0052 | 0.0007 | 0.0072 | 0.0011 | 0.0082 | 0.0001 | 0.0048 | 0.0012 | 0.016 | 0.0011 |
Punctate Nuclear | 0.0113 | 0.0003 | 0.0008 | 0.0002 | 0.0012 | 0.0011 | 0.0046 | 0.001 | 0.0024 | 0.0007 | 0.002 | 0.0025 | 0.0013 | 0.0008 | 0.003 | 0.0004 | 0.0029 | 0.0003 |
Vacuole | 0.0029 | 0.002 | 0.0023 | 0.001 | 0.0022 | 0.001 | 0.0056 | 0.0199 | 0.0046 | 0.0038 | 0.0102 | 0.0019 | 0.0036 | 0.0025 | 0.0035 | 0.0031 | 0.0016 | 0.0014 |
Vacuole Periphery | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0007 | 0.0004 | 0.0014 | 0.0033 | 0.0008 | 0.0007 | 0.0058 | 0.0003 | 0.0017 | 0.0002 | 0.0006 | 0.0006 | 0.0005 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.0786 | 8.74 | 10.5003 | 16.8983 | 10.2618 | 13.4941 | 17.9897 | 17.0412 | 17.1977 | 17.6482 |
Translational Efficiency | 0.7245 | 0.9392 | 0.8055 | 0.6685 | 0.7676 | 0.7536 | 0.689 | 0.58 | 0.7452 | 0.6826 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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