Standard name
Human Ortholog
Description Subunit of HIR nucleosome assembly complex; involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.08 0.11 0.12 0.18 0.13 0.11 0.06 0.06 0.06 0 0.05 0.14 0.12 0.09 0.05 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0.07 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0.43 0 0.12 0.1 0.36 0.4 0.3 0.68 0.69 0.65 0.5 0.15 0 0.06 0.08 0.08 0.18 0 0 0 0 0 0
Nucleus 0.79 0.8 0.82 0.78 0.74 0.61 0.64 0.69 0.65 0.61 0.64 0.77 0.83 0.76 0.78 0.74 0.8 0.74 0.6 0.61 0.49 0.49 0.35 0.41
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.15 0.07 0 0 0 0 0 0 0 0 0.08 0 0 0.05 0.06 0.07 0.07 0.05 0.2 0.15 0.2 0.19 0.22 0.19
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.05 0.17 0.09 0.14 0 0.09 0.11 0.05 0 0 0.07 0 0 0 0.08 0.07 0.06 0.06 0.06 0.07 0.06 0.1 0.11
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.11 0.13 0.14 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 1 1
Bud 0 1 1 2 1 2 7 1 3 12 3 7 1 0 1 0 0 1 1 0 1 2 6 12
Bud Neck 0 0 2 2 5 0 10 8 6 6 2 2 1 0 0 0 1 0 0 0 3 2 5 9
Bud Site 0 3 2 4 1 4 13 7 16 18 6 14 2 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 3 22 27 35 56 64 58 27 23 12 10 19 47 49 20 15 22 1 0 8 1 2 1
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 1 0 1 1 2 8 7 9 0 0 0 1 1 3
Endosome 0 0 5 0 4 5 6 6 1 1 2 3 2 4 8 11 13 7 5 5 15 12 26 22
Golgi 2 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 3 1
Mitochondria 32 49 11 30 29 115 192 153 285 250 141 148 55 13 26 18 24 56 1 4 8 6 17 11
Nucleus 107 92 235 187 213 195 302 353 271 222 140 228 304 248 321 166 250 230 95 91 176 130 145 174
Nuclear Periphery 1 0 0 0 0 2 0 2 4 4 1 1 2 3 1 2 5 1 1 0 2 3 5 5
Nucleolus 20 8 0 2 4 5 7 12 15 12 17 9 14 17 26 16 22 15 32 22 71 49 90 80
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1
SpindlePole 3 6 50 21 41 14 43 55 19 14 6 21 12 10 10 19 22 19 9 8 24 14 40 48
Vac/Vac Membrane 0 1 5 1 6 4 7 11 1 1 0 4 7 6 5 8 12 12 6 11 39 34 57 39
Unique Cell Count 136 115 286 241 286 318 475 515 418 361 218 297 368 327 412 224 313 310 158 150 363 267 414 428
Labelled Cell Count 171 163 334 277 339 403 651 666 648 563 331 447 420 350 451 268 371 374 158 150 363 267 414 428


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.1 5.4 4.8 5.5 4.4 4.5 5.0 3.9 3.8 3.7 4.1 4.8 4.9 4.8 6.8 7.1 6.7 4.6 4.6 5.0
Std Deviation (1e-4) 1.3 0.7 1.4 1.4 1.6 1.7 1.6 1.6 1.3 1.1 1.1 0.9 1.0 1.3 1.5 1.3 1.6 1.4 1.3 1.3 1.9
Intensity Change (Log2) – – – -0.17 0.03 -0.31 -0.26 -0.13 -0.46 -0.52 -0.54 -0.39 -0.17 -0.15 -0.17 0.33 0.4 0.31 -0.25 -0.23 -0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.3 1.5 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.705 3.096 2.8372 2.4315 2.1014 3.0027 0.6386 0.7853 0.5455 0.5624 0.3945 0.4622 0.6629 1.4888 2.7937 0.6477 10.7035 0.7822
Actin 0.0371 0.0026 0.0291 0.0001 0 0.0358 0.0104 0.0001 0.0007 0.0023 0.0009 0.0001 0.0244 0.0003 0.0078 0.0001 0.0025 0.0076
Bud 0.0003 0.0001 0.0007 0 0 0.0003 0.0005 0 0.0003 0.0002 0.0001 0.0001 0.001 0.0001 0.0006 0 0.0006 0.0002
Bud Neck 0.0024 0.0009 0.0045 0.0002 0.0001 0.0064 0.0036 0.0003 0.0007 0.0005 0.0023 0.0024 0.0018 0.0011 0.0305 0.0003 0.0051 0.0171
Bud Periphery 0.0004 0.0002 0.0008 0 0 0.0005 0.0011 0.0001 0.0006 0.0003 0.0001 0.0003 0.0022 0.0001 0.0013 0.0001 0.0055 0.0004
Bud Site 0.003 0.0004 0.0098 0.0001 0 0.0007 0.0042 0.0002 0.0055 0.0013 0.0003 0.0003 0.0078 0.0198 0.0132 0.0001 0.0021 0.0009
Cell Periphery 0.0003 0 0.0004 0 0 0.0001 0.0006 0 0.0002 0.0001 0 0.0001 0.0017 0.0007 0.0019 0.0001 0.0996 0.0035
Cytoplasm 0.015 0.0009 0.0015 0.0004 0.0002 0.0008 0.0089 0.0002 0.0007 0.0026 0.0041 0.0003 0.0044 0.001 0.0127 0.0053 0.002 0.0009
Cytoplasmic Foci 0.0185 0.0003 0.0177 0 0 0.0075 0.0237 0 0.0052 0.0008 0.0345 0.0003 0.0153 0.0006 0.004 0.0006 0.013 0.0041
Eisosomes 0.0004 0 0.0011 0 0 0.0002 0.0002 0 0 0.0001 0 0 0.0004 0.0002 0.0001 0.0001 0.008 0.0001
Endoplasmic Reticulum 0.009 0.0022 0.0004 0.0001 0 0.0006 0.0039 0.0001 0.0002 0.001 0.0005 0.0001 0.0033 0.0001 0.0014 0.0018 0.0034 0.0008
Endosome 0.0244 0.0012 0.0045 0 0 0.0179 0.0203 0.0001 0.0081 0.0054 0.0162 0.0012 0.0205 0.0002 0.0043 0.0028 0.0346 0.0119
Golgi 0.0087 0.0002 0.0069 0 0 0.0226 0.0055 0 0.0037 0.0005 0.0038 0.0008 0.0094 0 0.0016 0 0.0048 0.0097
Lipid Particles 0.0104 0.0003 0.0049 0 0 0.0113 0.0237 0 0.003 0.0006 0.0135 0.001 0.0223 0.0001 0.0011 0.0003 0.0193 0.006
Mitochondria 0.0212 0.0006 0.0234 0.0003 0.0001 0.0151 0.0051 0.0003 0.0021 0.0026 0.0006 0.0028 0.0057 0.0002 0.0012 0.0007 0.0052 0.0065
None 0.033 0.0034 0.0282 0.0001 0.0002 0.0007 0.0019 0.0001 0.0007 0.0037 0.0007 0.0001 0.0057 0.0003 0.0017 0.0513 0.0013 0.0008
Nuclear Periphery 0.0093 0.0392 0.0116 0.0076 0.011 0.0012 0.017 0.0116 0.0026 0.0103 0.0479 0.0031 0.022 0.0084 0.0156 0.0149 0.0206 0.0041
Nucleolus 0.0533 0.0506 0.0845 0.0256 0.0197 0.1147 0.0601 0.0711 0.0677 0.0659 0.1647 0.1321 0.0364 0.0618 0.0523 0.0285 0.0823 0.0839
Nucleus 0.6183 0.8295 0.7165 0.9617 0.9625 0.732 0.7668 0.9039 0.8801 0.8841 0.5949 0.8449 0.7688 0.821 0.7479 0.8403 0.5768 0.7934
Peroxisomes 0.0052 0.0001 0.013 0 0 0.0126 0.0055 0 0.003 0.0003 0.0233 0.0002 0.0042 0 0.0012 0 0.0012 0.0008
Punctate Nuclear 0.1278 0.0665 0.0394 0.0037 0.0058 0.0171 0.0283 0.0115 0.0123 0.0158 0.0894 0.0091 0.0397 0.0834 0.0951 0.0502 0.1044 0.0464
Vacuole 0.0014 0.0003 0.0007 0.0001 0 0.0011 0.0077 0.0001 0.0022 0.001 0.0015 0.0006 0.0021 0.0006 0.0038 0.0017 0.005 0.0006
Vacuole Periphery 0.0008 0.0006 0.0005 0.0001 0 0.0005 0.0014 0.0002 0.0004 0.0008 0.0004 0.0002 0.0011 0.0001 0.0008 0.0006 0.0029 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.535 6.0053 6.4058 12.1462 18.5087 10.0712 11.0055 9.8613 10.8298 16.0049
Translational Efficiency 1.0689 1.3858 1.0894 0.703 0.6329 1.5924 0.7569 0.7315 0.7418 0.8301

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of HIR nucleosome assembly complex; involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Hir2

Hir2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hir2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available