Standard name
Human Ortholog
Description Ubiquitin-binding protein; functions as ubiquitin-Atg8p adaptor in ubiquitin-dependent autophagy; serves as proteaphagy receptor for inactivated 26S proteasomes; contains CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; CUE5 has a paralog, DON1, that arose from the whole genome duplication; human TOLLIP is a functional CUE-domain homolog, can complement yeast null mutant, rescuing hypersensitivity of cue5 null mutant cells to Htt-96Q
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 0.05 0.21 0.12 0.1 0.12 0.1 0.07 0.11 0.13 0.09 0.1 0.07 0.09 0 0 0 0.07 0 0 0.1 0.07 0.08 0.12
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.1 0 0 0 0.05 0.06 0.1 0.08 0.05 0.06 0 0.07 0.07 0 0.08 0 0 0 0 0 0 0
Cytoplasm 0.78 0.87 0.44 0.54 0.62 0.49 0.41 0.4 0.33 0.32 0.31 0.31 0.7 0.72 0.85 0.88 0.79 0.78 0.52 0.56 0.3 0.31 0.31 0.26
Endoplasmic Reticulum 0.08 0.12 0.28 0.23 0.22 0.23 0.22 0.2 0.25 0.26 0.13 0.16 0.27 0.23 0.12 0 0.05 0.06 0.15 0.17 0.22 0.18 0.28 0.29
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0
Golgi 0 0 0.19 0.21 0.07 0.09 0.07 0.05 0.09 0 0.05 0 0 0.06 0 0.08 0.1 0.05 0.13 0.1 0.24 0.13 0.19 0.19
Mitochondria 0.12 0 0 0.12 0.1 0.34 0.45 0.46 0.42 0.49 0.59 0.56 0 0 0 0 0 0 0 0 0.06 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0.09 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 6 55 17 23 35 41 23 25 47 24 31 9 9 5 4 8 10 3 5 31 4 9 12
Bud 0 0 2 2 6 4 8 8 1 12 6 10 0 1 1 0 1 0 1 1 0 0 3 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 26 4 5 11 20 19 23 29 14 19 3 7 10 6 14 6 0 0 10 0 0 0
Cytoplasm 71 110 117 76 150 142 162 130 74 119 82 96 93 71 129 148 145 113 53 73 93 21 36 29
Endoplasmic Reticulum 7 15 75 32 52 68 87 64 57 96 33 50 36 23 18 6 9 9 15 22 70 12 32 32
Endosome 1 0 1 0 3 2 0 2 3 0 1 2 0 0 0 0 2 2 1 2 8 5 3 3
Golgi 2 1 50 29 18 26 26 16 21 12 13 13 2 6 6 14 19 7 13 13 75 8 21 21
Mitochondria 11 3 12 17 25 98 179 150 96 180 154 174 0 0 0 0 8 1 3 2 19 3 3 4
Nucleus 1 1 0 0 2 0 0 1 0 0 2 0 0 0 1 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 3 0 0 0 0 1 0 1 0 0 0 0 0
Nucleolus 2 1 1 0 0 0 2 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0
Vac/Vac Membrane 0 0 3 1 1 1 5 9 6 10 4 10 1 1 0 3 7 12 2 6 1 6 4 4
Unique Cell Count 91 127 268 140 240 290 399 326 226 367 262 313 132 99 151 169 184 144 102 131 315 70 116 112
Labelled Cell Count 103 138 343 178 285 387 531 422 306 505 333 409 144 118 170 181 215 160 102 131 315 70 116 112


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.5 15.1 13.6 12.5 12.6 11.9 11.2 11.4 11.5 11.0 10.0 10.5 15.4 15.3 15.1 25.6 22.5 22.8 – 16.4 16.1
Std Deviation (1e-4) 2.5 2.7 1.8 2.0 1.8 1.8 2.1 2.2 2.4 2.4 2.5 2.5 2.4 2.3 2.5 7.2 6.5 6.7 – 2.3 2.9
Intensity Change (Log2) – – – -0.12 -0.11 -0.19 -0.27 -0.25 -0.23 -0.3 -0.43 -0.37 0.18 0.17 0.15 0.92 0.73 0.75 – 0.28 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.1 -3.4 -2.7 -3.7 -4.8 -2.8 -2.6 -3.7 -3.6 -3.5 -2.6 -4.8 -5.4 -4.9 -3.6
Bud 0 0 0 0 0 0 2.1 0 2.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.5 -3.6 -2.8 -2.3 -1.8 0.2 -0.8 -1.9 -1.6 -2.7 -0.8 -1.1 -2.4 -0.8 -2.0
Cytoplasm 2.0 4.2 1.3 -0.8 -0.9 -2.5 -2.9 -2.9 -3.2 5.0 4.8 8.3 9.2 7.4 6.8
Endoplasmic Reticulum -1.1 -1.6 -1.2 -1.8 -2.4 -0.7 -0.5 -4.4 -3.5 -0.1 -0.9 -3.8 -6.4 -6.2 -5.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.5 -3.7 -3.3 -4.8 -5.3 -3.0 -6.5 -4.9 -5.6 -4.8 -3.0 -4.2 -3.0 -2.4 -3.9
Mitochondria 2.9 2.6 8.7 11.3 11.3 10.2 12.1 13.5 13.2 0 0 0 0 -0.1 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 1.4 0 1.4 0 1.7 0 0 0 0 0 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.4555 11.2564 10.343 9.5178 8.7185 10.283 8.5897 12.084 11.0527 10.0467 9.6408 10.7956 9.0941 11.9135 10.2953 9.5871 8.8725 10.3226
Actin 0.0623 0.0195 0.0431 0.1134 0.0665 0.0415 0.0465 0.0137 0.0243 0.0143 0.0792 0.0723 0.0325 0.0299 0.0665 0.0359 0.1409 0.0525
Bud 0.0024 0.0026 0.0025 0.0036 0.0023 0.0017 0.0012 0.002 0.0021 0.0014 0.0016 0.0014 0.0022 0.003 0.0047 0.006 0.0013 0.001
Bud Neck 0.0018 0.0009 0.0015 0.0014 0.0026 0.0096 0.0016 0.0007 0.0022 0.0024 0.0015 0.0205 0.0016 0.0008 0.0032 0.0024 0.0021 0.0105
Bud Periphery 0.0027 0.0013 0.0014 0.0043 0.0023 0.0021 0.0009 0.0007 0.0014 0.0011 0.0011 0.0025 0.0024 0.0009 0.0021 0.0037 0.0007 0.0009
Bud Site 0.003 0.0065 0.0026 0.0033 0.002 0.0008 0.004 0.0062 0.0073 0.0011 0.0008 0.0011 0.0021 0.0066 0.0087 0.0008 0.0014 0.0005
Cell Periphery 0.0018 0.0004 0.0006 0.001 0.0008 0.0008 0.002 0.0005 0.0007 0.0005 0.0004 0.0016 0.0011 0.0007 0.0007 0.0006 0.0004 0.0005
Cytoplasm 0.2766 0.3666 0.3566 0.2642 0.1678 0.1954 0.3399 0.5838 0.5287 0.4296 0.3508 0.3157 0.3439 0.4867 0.4012 0.3599 0.1035 0.202
Cytoplasmic Foci 0.0717 0.0987 0.0756 0.0579 0.1013 0.0746 0.0765 0.0692 0.0804 0.1399 0.0869 0.07 0.0708 0.079 0.0829 0.0908 0.0821 0.0676
Eisosomes 0.0004 0.0001 0.0001 0.0014 0.0004 0.0005 0.0005 0 0.0001 0.0001 0.0001 0.0007 0.0003 0.0001 0.0005 0.0002 0.0002 0.0002
Endoplasmic Reticulum 0.0427 0.0111 0.0133 0.0069 0.0132 0.0182 0.0241 0.0132 0.0254 0.0226 0.0156 0.0329 0.0646 0.0113 0.0168 0.0129 0.0111 0.0408
Endosome 0.2034 0.2245 0.2653 0.2635 0.2446 0.222 0.2519 0.1913 0.1927 0.23 0.1772 0.1838 0.2054 0.1911 0.2155 0.3267 0.1711 0.2393
Golgi 0.2713 0.2446 0.2033 0.2285 0.3538 0.3763 0.168 0.1033 0.0922 0.1255 0.1813 0.2464 0.2062 0.1748 0.1457 0.1154 0.4466 0.3408
Lipid Particles 0.0011 0.0006 0.0022 0.0006 0.0007 0.0005 0.0192 0.0016 0.0039 0.0013 0.0036 0.0026 0.0042 0.0004 0.0073 0.0058 0.0037 0.0016
Mitochondria 0.0435 0.0134 0.0183 0.0315 0.0299 0.0477 0.026 0.0043 0.0142 0.006 0.056 0.0388 0.0266 0.0072 0.02 0.006 0.0102 0.0291
None 0.0012 0.0006 0.0007 0.0005 0.0015 0.0005 0.0053 0.0005 0.0021 0.0006 0.0006 0.0009 0.0023 0.0008 0.0017 0.0009 0.0003 0.0008
Nuclear Periphery 0.002 0.0006 0.0007 0.0008 0.0009 0.0005 0.0058 0.0007 0.0013 0.0008 0.0005 0.001 0.0103 0.0005 0.0028 0.0025 0.0003 0.0039
Nucleolus 0 0 0 0 0 0 0.0004 0 0.0001 0 0 0.0001 0.0002 0.0001 0.0002 0.0001 0 0.0001
Nucleus 0.0006 0.0004 0.0004 0.0004 0.0004 0.0003 0.0056 0.0004 0.0007 0.0005 0.0003 0.0005 0.0039 0.0005 0.001 0.0006 0.0001 0.0005
Peroxisomes 0.0006 0.0013 0.0014 0.0008 0.001 0.0007 0.009 0.0007 0.003 0.0052 0.0328 0.0021 0.0015 0.0006 0.0042 0.0017 0.0202 0.0011
Punctate Nuclear 0.0002 0.0001 0.0003 0.0002 0.0001 0.0001 0.0028 0.0001 0.0046 0.0079 0.0033 0.0003 0.0017 0.0002 0.001 0.001 0.0005 0.0011
Vacuole 0.0068 0.0038 0.0063 0.0073 0.0045 0.0032 0.0055 0.0048 0.0082 0.0059 0.0033 0.0026 0.0128 0.0031 0.0094 0.0192 0.0017 0.0024
Vacuole Periphery 0.004 0.0023 0.0038 0.0085 0.0033 0.0032 0.0035 0.0021 0.0045 0.0033 0.003 0.0025 0.0036 0.0019 0.0038 0.0067 0.0014 0.0029

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 56.2415 35.9118 55.6649 67.3306 59.0301 47.2881 55.0707 67.2582 74.1963 58.0455
Translational Efficiency 1.7573 2.3429 1.756 1.7313 1.6317 2.2017 1.4368 1.6927 1.6778 1.5335

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ubiquitin-binding protein; functions as ubiquitin-Atg8p adaptor in ubiquitin-dependent autophagy; serves as proteaphagy receptor for inactivated 26S proteasomes; contains CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; CUE5 has a paralog, DON1, that arose from the whole genome duplication; human TOLLIP is a functional CUE-domain homolog, can complement yeast null mutant, rescuing hypersensitivity of cue5 null mutant cells to Htt-96Q
Localization
Cell Percentages cytoplasm (32%), Golgi (0%), mixed (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cue5

Cue5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cue5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available