Standard name | |
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Human Ortholog | |
Description | Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | 5 | 1 | 1 | 2 | 6 | 4 |
Bud | 1 | 1 | 1 | 2 | 1 | 2 | 3 | 9 | – | 6 | 3 | 6 | 0 | 0 | 1 | – | – | – | 5 | 1 | 0 | 0 | 3 | 8 |
Bud Neck | 2 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | – | 2 | 0 | 0 | 3 | 0 | 3 | – | – | – | 3 | 0 | 1 | 0 | 7 | 10 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 6 | – | 2 | 3 | 2 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | – | 1 | 0 | 0 | 1 | 0 | 0 | – | – | – | 1 | 0 | 0 | 0 | 3 | 2 |
Cytoplasm | 368 | 110 | 81 | 83 | 96 | 137 | 82 | 147 | – | 79 | 63 | 70 | 287 | 267 | 386 | – | – | – | 314 | 96 | 57 | 45 | 123 | 118 |
Endoplasmic Reticulum | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 1 | 1 | 0 | – | – | – | 5 | 0 | 1 | 2 | 4 | 2 |
Endosome | 2 | 2 | 0 | 0 | 3 | 0 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | 1 | – | – | – | 10 | 3 | 2 | 0 | 4 | 5 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | 0 | 1 | 0 | 1 | 0 | 0 |
Mitochondria | 6 | 12 | 12 | 17 | 20 | 74 | 76 | 151 | – | 103 | 116 | 139 | 0 | 0 | 0 | – | – | – | 40 | 15 | 33 | 45 | 88 | 74 |
Nucleus | 0 | 1 | 1 | 0 | 1 | 2 | 0 | 5 | – | 0 | 0 | 1 | 1 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 2 | 0 |
Nucleolus | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 2 | 0 | 1 | 0 | 5 | 5 | 6 | – | 6 | 6 | 11 | 0 | 0 | 3 | – | – | – | 8 | 1 | 5 | 1 | 7 | 6 |
Vac/Vac Membrane | 6 | 1 | 0 | 1 | 2 | 0 | 2 | 2 | – | 1 | 2 | 2 | 1 | 0 | 3 | – | – | – | 10 | 2 | 1 | 12 | 21 | 36 |
Unique Cell Count | 381 | 124 | 91 | 93 | 111 | 184 | 132 | 255 | 153 | 157 | 179 | 291 | 268 | 393 | 422 | 128 | 108 | 118 | 278 | 282 | ||||
Labelled Cell Count | 393 | 133 | 96 | 105 | 125 | 224 | 169 | 329 | 200 | 194 | 233 | 296 | 268 | 398 | 422 | 128 | 108 | 118 | 278 | 282 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 4.0 | 3.2 | 3.4 | 4.0 | 3.6 | 2.9 | 3.2 | – | 2.7 | 3.1 | 2.8 | 5.2 | 4.8 | 4.9 | – | – | – | 5.9 | 5.4 | 5.5 |
Std Deviation (1e-4) | 0.8 | 0.9 | 0.6 | 1.1 | 2.5 | 2.1 | 1.2 | 1.9 | – | 1.1 | 2.0 | 1.7 | 1.1 | 1.0 | 1.3 | – | – | – | 2.1 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.29 | 0.13 | -0.18 | -0.01 | – | -0.25 | -0.07 | -0.2 | 0.67 | 0.57 | 0.6 | – | – | – | 0.87 | 0.74 | 0.77 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.7772 | -0.258 | -0.5436 | -0.6338 | -0.7395 | -0.4008 | 1.8205 | 1.5972 | 1.999 | 2.5396 | 2.2949 | 2.2469 | -0.6672 | -0.6296 | -0.4695 | -0.4251 | 0.7137 | -0.4815 |
Actin | 0.001 | 0.0012 | 0.0007 | 0.0003 | 0.0001 | 0.0004 | 0.0457 | 0.0008 | 0.0214 | 0.0025 | 0.0166 | 0.0036 | 0.0486 | 0 | 0.0364 | 0.0648 | 0.0543 | 0.0002 |
Bud | 0.0002 | 0.0002 | 0.0033 | 0.0001 | 0.0001 | 0.0006 | 0.0043 | 0.0011 | 0.0003 | 0.0004 | 0.0108 | 0.0004 | 0 | 0 | 0.0019 | 0.0001 | 0.0012 | 0 |
Bud Neck | 0.0021 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0051 | 0.0022 | 0 | 0.0002 | 0.0008 | 0.0061 | 0.0004 | 0.0007 | 0 | 0.0014 | 0.0002 | 0.0006 | 0.0001 |
Bud Periphery | 0.0002 | 0.0003 | 0.0031 | 0.0003 | 0.0001 | 0.0005 | 0.0123 | 0.0074 | 0.0012 | 0.0008 | 0.0248 | 0.0019 | 0.0001 | 0 | 0.0032 | 0.0001 | 0.0008 | 0 |
Bud Site | 0.0031 | 0.0025 | 0.0051 | 0.0001 | 0 | 0.0002 | 0.0023 | 0.0004 | 0.0004 | 0.0006 | 0.0214 | 0.0001 | 0.0002 | 0.0002 | 0.0062 | 0.0001 | 0.0017 | 0 |
Cell Periphery | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0014 | 0.001 | 0.0006 | 0.0001 | 0.0011 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0474 | 0.0658 | 0.0531 | 0.1155 | 0.0634 | 0.0801 | 0.0723 | 0.0761 | 0.0828 | 0.186 | 0.1094 | 0.1606 | 0.0546 | 0.1082 | 0.0776 | 0.0984 | 0.059 | 0.1458 |
Cytoplasmic Foci | 0.0157 | 0.0086 | 0.0118 | 0.0054 | 0.0062 | 0.0121 | 0.0093 | 0.0084 | 0.0078 | 0.042 | 0.0304 | 0.0054 | 0.0053 | 0.002 | 0.0159 | 0.0042 | 0.0351 | 0.0022 |
Eisosomes | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0005 | 0.0002 | 0.0006 | 0.0012 | 0.0005 | 0.0002 | 0.0005 | 0.0003 | 0.0004 | 0.0001 |
Endoplasmic Reticulum | 0.0008 | 0.0011 | 0.0007 | 0.0015 | 0.0005 | 0.0009 | 0.0104 | 0.0111 | 0.0209 | 0.0008 | 0.0485 | 0.0268 | 0.0023 | 0.0013 | 0.0023 | 0.0015 | 0.0071 | 0.0011 |
Endosome | 0.0017 | 0.0019 | 0.001 | 0.0011 | 0.0027 | 0.0033 | 0.0067 | 0.0039 | 0.0028 | 0.0111 | 0.0221 | 0.0012 | 0.0013 | 0.0008 | 0.0099 | 0.0013 | 0.0394 | 0.0004 |
Golgi | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0012 | 0.0004 | 0.0007 | 0.0065 | 0.0041 | 0.0003 | 0.0005 | 0 | 0.0112 | 0.0026 | 0.0183 | 0 |
Lipid Particles | 0.0015 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0012 | 0.0022 | 0.0008 | 0.0006 | 0.0135 | 0.0025 | 0.0005 | 0.0011 | 0.0002 | 0.0034 | 0.0014 | 0.0758 | 0.0001 |
Mitochondria | 0.0003 | 0.0007 | 0.0006 | 0.0011 | 0.0004 | 0.0005 | 0.0011 | 0.0034 | 0.0007 | 0.006 | 0.0105 | 0.0009 | 0.0003 | 0 | 0.0043 | 0.0004 | 0.0049 | 0.0001 |
None | 0.9178 | 0.9091 | 0.9122 | 0.871 | 0.9228 | 0.8856 | 0.8107 | 0.8762 | 0.8535 | 0.7006 | 0.6638 | 0.7936 | 0.8804 | 0.8863 | 0.7862 | 0.8195 | 0.627 | 0.8492 |
Nuclear Periphery | 0.0006 | 0.0007 | 0.0005 | 0.0005 | 0.0004 | 0.0004 | 0.0016 | 0.0048 | 0.0012 | 0.0003 | 0.0084 | 0.0008 | 0.001 | 0.0001 | 0.0066 | 0.0003 | 0.0202 | 0.0001 |
Nucleolus | 0.0004 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0008 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0021 | 0.0003 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0005 | 0 |
Nucleus | 0.0012 | 0.0019 | 0.0013 | 0.0008 | 0.0005 | 0.0012 | 0.0012 | 0.0014 | 0.0007 | 0.0004 | 0.0053 | 0.0006 | 0.0009 | 0.0001 | 0.0251 | 0.0003 | 0.0085 | 0.0002 |
Peroxisomes | 0.0011 | 0.0006 | 0.0007 | 0.0004 | 0.0001 | 0.0005 | 0.0102 | 0.0004 | 0.0004 | 0.0251 | 0.0031 | 0.0003 | 0.0002 | 0 | 0.0029 | 0.0033 | 0.0276 | 0 |
Punctate Nuclear | 0.0034 | 0.0028 | 0.0035 | 0.0006 | 0.0006 | 0.0055 | 0.0015 | 0.0003 | 0.001 | 0.0008 | 0.0046 | 0.0005 | 0.0008 | 0.0001 | 0.0023 | 0.0004 | 0.0143 | 0.0001 |
Vacuole | 0.0006 | 0.0009 | 0.0006 | 0.0004 | 0.0013 | 0.0005 | 0.0024 | 0.0013 | 0.0019 | 0.001 | 0.0024 | 0.0004 | 0.0007 | 0.0002 | 0.0012 | 0.0005 | 0.0018 | 0.0002 |
Vacuole Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0006 | 0.0003 | 0.0002 | 0.0014 | 0.0001 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0013 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 97.8827 | 116.6681 | 59.0269 | 102.8619 | 103.8347 | 47.432 | 100.5367 | 166.4721 | 121.8448 | 155.1717 |
Translational Efficiency | 3.4799 | 2.2007 | 4.1576 | 1.3162 | 2.4609 | 9.9655 | 3.5645 | 2.6307 | 3.1491 | 2.4526 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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