Standard name
Human Ortholog
Description Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus, remodeling messenger ribonucleoprotein particles (mRNPs), with ATPase activity stimulated by Gle1p, IP6 and Nup159p; involved in translation termination along with Sup45p (eRF1); role in the cellular response to heat stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0.08 0.11 0.17 0.14 0.12 0.24 0.13 0.21 0.21 0.13 0.14 0.13 0.21 0.19 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0.06 0.12 0.06 0.05 0 0.12 0 0.09 0.05 0.05 0 0.07 0.16 0.14 0.07 0 0 0 0.06 0.09 0.08 0.09 0.09 0.07
Endosome 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0.06 0.09 0.17
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.15 0.16 0.33 0.39 0.46 0 0 0 0 0.1 0.09
Mitochondria 0 0 0.12 0.12 0.1 0.24 0.32 0.2 0.34 0.39 0.47 0.48 0 0 0 0.07 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Nuclear Periphery 0.19 0.25 0.31 0.46 0.32 0.32 0.37 0.33 0.29 0.38 0.23 0.31 0.17 0.13 0.05 0 0 0 0.78 0.51 0.79 0.66 0.57 0.47
Nucleolus 0.1 0.12 0.06 0.14 0.08 0.09 0.1 0.1 0.09 0.05 0.08 0.09 0.05 0.07 0.05 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.76 0.75 0.62 0.52 0.62 0.28 0.41 0.36 0.22 0.26 0.12 0.15 0.65 0.58 0.68 0.52 0.59 0.64 0.05 0.19 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 1 0 1 1 1 1 1 0 0 3 9 9 15 2 3 1 0 0 5 7 32
Bud 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 2 2 0 4 8 26
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 5 1 0 5 10 19 20 25 35 24 15 32 34 56 71 54 46 17 2 1 0 2 3 3
Endoplasmic Reticulum 9 2 1 3 3 14 4 18 8 10 1 10 41 56 39 9 8 2 10 5 5 17 45 42
Endosome 0 0 0 2 0 7 3 11 3 1 1 1 1 10 24 4 0 0 4 1 3 11 43 100
Golgi 2 0 0 1 1 4 1 3 1 0 1 2 42 62 86 87 96 68 1 0 0 7 49 55
Mitochondria 5 0 2 8 9 27 46 42 50 72 34 73 0 0 1 17 9 5 0 0 1 1 4 10
Nucleus 1 0 0 1 1 5 1 4 8 8 3 4 2 1 2 1 1 0 3 3 0 0 2 3
Nuclear Periphery 28 4 5 30 29 36 53 67 42 70 17 47 43 53 27 7 3 3 147 33 53 126 273 281
Nucleolus 15 2 1 9 7 10 15 21 13 10 6 13 12 28 25 7 5 3 2 1 0 1 4 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2 4
SpindlePole 0 0 0 0 0 0 0 2 0 0 0 0 0 0 2 0 1 0 0 1 0 1 5 5
Vac/Vac Membrane 113 12 10 34 57 31 59 75 33 48 9 23 165 241 357 137 146 95 9 12 0 7 25 26
Unique Cell Count 149 16 16 65 92 112 145 206 147 186 73 151 255 414 526 261 247 149 189 66 68 193 482 605
Labelled Cell Count 178 22 19 94 117 154 203 269 195 246 88 207 343 517 644 339 317 196 189 66 68 193 482 605


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.7 18.4 13.0 12.6 12.0 10.4 9.6 9.8 9.2 9.2 8.0 8.3 21.3 20.6 20.4 22.1 25.3 30.5 20.0 19.8 20.7
Std Deviation (1e-4) 3.9 4.7 2.9 3.4 2.9 2.7 2.5 2.4 2.3 2.1 2.1 2.1 5.0 4.5 4.7 6.5 7.5 7.5 4.5 4.1 4.3
Intensity Change (Log2) – – – -0.05 -0.11 -0.32 -0.43 -0.4 -0.49 -0.51 -0.7 -0.65 0.71 0.66 0.65 0.76 0.96 1.23 0.62 0.61 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 2.8 3.1 3.5
Mitochondria 0 0 0 0 0 1.8 2.1 2.5 2.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1.1 0 0.1 0.4 0.1 0 0.5 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.7 0 -2.8 -1.7 -2.1 0 0 0 0 0.2 -0.3 0.5 -0.8 -0.3 0.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.5665 16.8899 16.7192 15.7612 17.1848 16.0576 12.9463 13.1523 12.7349 11.8698 10.6437 11.9062 14.8184 17.3042 15.2752 15.1367 14.3482 14.9917
Actin 0.0139 0.0034 0.0093 0.0022 0.0002 0.0004 0.0122 0.0347 0.0046 0.0175 0.0049 0.0035 0.0358 0.0011 0.0088 0.0265 0.0006 0.0103
Bud 0.0001 0.0003 0.0002 0 0 0 0.0002 0.0002 0.0002 0.0003 0.0069 0.0002 0.0001 0 0.0001 0.0004 0 0.0001
Bud Neck 0.0004 0.0003 0.0005 0.0006 0 0.0002 0.0003 0.0004 0.0003 0.0005 0.0011 0.0002 0.0003 0.0001 0.0002 0.0011 0 0.0002
Bud Periphery 0.0003 0.0008 0.0002 0.0001 0 0.0001 0.0004 0.0005 0.0006 0.0008 0.0074 0.0003 0.0002 0.0001 0.0003 0.0015 0 0.0002
Bud Site 0.0002 0.0007 0.0021 0 0 0 0.0035 0.0018 0.0006 0.0004 0.042 0.0005 0.0006 0.0001 0.0003 0.0006 0 0.0001
Cell Periphery 0.0001 0.0013 0.0001 0.0001 0 0 0.0002 0.0003 0.0002 0.0002 0.0008 0.0001 0.0001 0.0001 0.0001 0.0005 0 0
Cytoplasm 0.0055 0.0384 0.009 0.0044 0.0074 0.0031 0.0112 0.0132 0.0028 0.0013 0.0262 0.0022 0.0057 0.0053 0.0009 0.0071 0.0002 0.0004
Cytoplasmic Foci 0.0057 0.0004 0.0012 0.0002 0.0001 0.0001 0.0227 0.0028 0.0095 0.0013 0.0114 0.0032 0.0187 0.0004 0.0076 0.0168 0.0001 0.0023
Eisosomes 0.0002 0.0006 0.0001 0 0 0 0.0002 0.0005 0.0002 0.0001 0.0002 0.0001 0.0002 0 0.0001 0.0002 0 0.0001
Endoplasmic Reticulum 0.0148 0.0228 0.0202 0.0246 0.0033 0.0033 0.0096 0.0314 0.0194 0.0284 0.0043 0.0083 0.015 0.0395 0.0262 0.0766 0.0113 0.0054
Endosome 0.0198 0.0027 0.0042 0.0035 0.0014 0.0013 0.0273 0.008 0.0308 0.0113 0.0349 0.0184 0.0219 0.0083 0.0064 0.0058 0.0043 0.0041
Golgi 0.0024 0.0004 0.0008 0.0007 0.0005 0.0001 0.0063 0.0049 0.0097 0.0043 0.0051 0.0071 0.0033 0.001 0.0018 0.0026 0.0005 0.0028
Lipid Particles 0.0034 0.0007 0.0012 0.0014 0.0022 0.0004 0.0155 0.011 0.017 0.0054 0.0014 0.0181 0.0195 0.0058 0.0044 0.0027 0.0009 0.0063
Mitochondria 0.0025 0.0025 0.0007 0.0007 0.0002 0.0003 0.0037 0.0026 0.003 0.0055 0.044 0.0181 0.0019 0.0011 0.0011 0.0031 0.0006 0.0018
None 0.0029 0.0002 0.0003 0.0001 0.0001 0.0001 0.0175 0.0059 0.0232 0.0005 0.0005 0.0005 0.0046 0.0002 0.0008 0.0013 0.0001 0.0003
Nuclear Periphery 0.8524 0.8084 0.8742 0.8442 0.8934 0.9407 0.8132 0.8128 0.812 0.8513 0.7703 0.8917 0.8155 0.8609 0.8851 0.7436 0.9537 0.9317
Nucleolus 0.0007 0.0043 0.0006 0.0003 0.0002 0.0003 0.0003 0.0003 0.001 0.001 0.0009 0.0002 0.0008 0.0005 0.0002 0.0003 0.0001 0.0002
Nucleus 0.0343 0.0889 0.0531 0.0952 0.0727 0.0362 0.0114 0.0101 0.0136 0.0201 0.0036 0.0055 0.0089 0.0391 0.0151 0.0539 0.0074 0.0128
Peroxisomes 0.0003 0 0.0002 0 0 0 0.0066 0.0004 0.0047 0.0002 0.001 0.0005 0.0026 0 0.0017 0.0114 0 0.0032
Punctate Nuclear 0.0137 0.0024 0.0021 0.002 0.0014 0.0014 0.0022 0.0021 0.0056 0.007 0.0008 0.0008 0.01 0.003 0.0059 0.005 0.0009 0.0016
Vacuole 0.003 0.0045 0.0018 0.002 0.0007 0.0013 0.0022 0.0039 0.0037 0.0043 0.0092 0.0015 0.0044 0.006 0.0029 0.0031 0.0009 0.0011
Vacuole Periphery 0.0235 0.0161 0.018 0.0177 0.0159 0.0107 0.0333 0.0522 0.0373 0.0384 0.0231 0.0189 0.0299 0.0272 0.0299 0.036 0.0184 0.0149

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 111.5588 106.1937 78.2952 104.9463 109.5914 84.479 96.0157 87.3346 80.8701 103.2363
Translational Efficiency 1.87 2.0253 2.3243 1.5438 1.6927 2.4511 1.7615 1.7943 2.0011 1.7059

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus, remodeling messenger ribonucleoprotein particles (mRNPs), with ATPase activity stimulated by Gle1p, IP6 and Nup159p; involved in translation termination along with Sup45p (eRF1); role in the cellular response to heat stress
Localization
Cell Percentages nuclear periphery (72%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Dbp5

Dbp5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dbp5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available