Standard name
Human Ortholog
Description Nuclear protein that inhibits replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.07 0 0 0 0 0 0 0 0.09 0.13 0.16 0 0 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.09 0.05 0.09 0.15 0.18 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.94 0.95 0.93 0.88 0.82 0.83 0.82 0.79 0.77 0.76 0.75 0.9 0.83 0.76 0.85 0.89 0.83 0.93 0.94 0.93 0.85 0.7 0.59
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.06 0.18 0.21 0.22 0.29 0.25 0.31 0.3 0 0 0 0.06 0.05 0.09 0 0 0 0 0.09 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0.08 0.18 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 0 0 0 0 0 2 2 1 1 4 0 0 1 0 0 1 0 4 1 0 1 3 3
Bud Neck 0 0 0 0 0 0 1 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 3 7 9 12 12 22 15 13 0 0 1 0 2 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 4 7 10 18 7 13 15 7 9 5 6 28 59 77 12 10 17 0 0 0 2 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 5 0 0 0 0 0 0
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 1 1 5 1 0 2 4 1 1 0 2 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5
Mitochondria 7 6 2 4 9 39 44 27 30 54 63 63 0 0 0 5 4 9 0 1 3 4 3 5
Nucleus 291 202 301 279 242 247 387 401 265 286 273 323 284 381 361 248 267 249 298 209 303 270 287 251
Nuclear Periphery 2 0 1 1 0 4 5 3 5 7 2 4 0 1 2 2 1 4 0 0 0 0 1 2
Nucleolus 9 5 9 12 16 55 96 106 97 93 110 128 0 4 14 18 14 26 4 2 3 6 36 39
Peroxisomes 0 0 0 0 0 1 0 0 1 1 1 0 0 2 2 0 0 2 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 2 0 0 0 1 9 15 0 0 2 0 0 1 0 2 1
Vac/Vac Membrane 2 2 2 2 4 2 1 2 3 4 0 3 4 5 12 19 14 9 3 4 9 26 73 104
Unique Cell Count 311 215 317 300 276 303 466 491 334 371 357 431 317 457 476 293 299 300 320 223 326 317 413 424
Labelled Cell Count 316 219 323 308 292 362 558 570 423 478 473 540 318 464 490 307 314 325 320 223 326 317 413 424


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.5 12.0 12.5 10.8 10.2 7.8 7.9 8.2 7.7 7.4 6.8 6.8 15.0 14.1 12.7 20.2 19.1 18.9 15.6 15.8 16.5
Std Deviation (1e-4) 3.3 3.7 3.4 3.2 3.4 2.5 2.8 2.6 2.5 2.5 2.2 2.2 5.5 5.7 5.7 8.3 7.1 7.6 5.5 5.0 4.5
Intensity Change (Log2) – – – -0.2 -0.29 -0.67 -0.65 -0.61 -0.69 -0.75 -0.88 -0.87 0.26 0.18 0.03 0.7 0.61 0.6 0.32 0.34 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.4 4.4 3.7 3.1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 2.6 0.1 0.5 0.7 -0.1 0.2 -0.8 -0.8 3.7 5.2 6.3 1.3 0.9 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.4 6.1 5.1 3.6 4.9 6.7 7.5 6.7 0 0 0 0 0 2.2
Nucleus -1.0 -3.2 -5.2 -5.0 -5.5 -5.9 -6.6 -6.7 -7.3 -2.5 -4.9 -7.1 -4.2 -2.6 -4.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.8 1.8 6.3 7.2 7.4 9.1 8.2 9.5 9.4 0 -2.1 0.1 2.0 1.2 3.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 3.2 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 2.0 4.0 3.2 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.3676 15.4412 14.6317 13.8732 14.287 14.457 13.1509 16.3257 15.6236 14.6029 10.7023 14.541 17.0733 15.4932 14.0216 13.6355 12.644 13.521
Actin 0 0 0.0002 0 0 0 0.0133 0 0.0003 0 0.0109 0.0064 0.0005 0 0.0001 0 0.0021 0.0021
Bud 0.0003 0 0 0.0002 0.0001 0 0.0031 0 0 0.0009 0.001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0001 0.0001 0.0001 0.0001 0.0002 0.0004 0.0004 0 0 0.0004 0.0007 0.0005 0.0001 0 0.0001 0.0001 0.0003 0.0005
Bud Periphery 0.0005 0 0.0001 0.0003 0.0002 0.0001 0.0036 0 0.0001 0.0019 0.0023 0.0001 0.0002 0 0.0002 0.0002 0.0001 0.0003
Bud Site 0.0002 0 0.0004 0.0001 0 0.0001 0.0015 0 0.0002 0.0003 0.0035 0.0001 0.0003 0 0.0003 0.0001 0.0004 0.0001
Cell Periphery 0.0004 0 0.0001 0.0004 0.0001 0.0001 0.001 0 0 0.0009 0.0026 0.0001 0.0002 0 0.0002 0.0002 0.0001 0.0002
Cytoplasm 0.0001 0 0.0007 0.0001 0.0001 0.0001 0.0071 0.0003 0.0001 0.0003 0.0005 0.0002 0.0011 0 0.0012 0 0.0002 0.0001
Cytoplasmic Foci 0.0019 0 0.0033 0 0 0.0015 0.0413 0.0035 0.0009 0.0004 0.0172 0.001 0.0015 0 0.0019 0 0.0139 0.0003
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.0001 0.0003 0 0 0 0.003 0 0.0002 0 0.0004 0.0003 0.0004 0 0.0001 0 0.0002 0.0001
Endosome 0.0008 0 0.0028 0 0 0.0002 0.0222 0.0001 0.0121 0.0004 0.0138 0.0029 0.0045 0 0.0002 0 0.004 0.0013
Golgi 0.0002 0 0.0028 0 0 0 0.0135 0 0.0027 0 0.0209 0.0013 0.0003 0 0 0 0.0028 0.0006
Lipid Particles 0.0029 0 0.0042 0.0001 0 0.0007 0.0255 0.0008 0.0027 0.0003 0.0475 0.0022 0.0009 0 0.001 0 0.0336 0.001
Mitochondria 0.0012 0.0001 0.0012 0.0007 0.0011 0.0002 0.0126 0.0005 0.0029 0.0007 0.0278 0.0006 0.0016 0.0001 0.0045 0.0007 0.0009 0.0045
None 0 0 0.0006 0.0001 0 0.0001 0.0026 0.0006 0.0001 0.0001 0.0004 0.0005 0.001 0 0.001 0 0.0003 0.0001
Nuclear Periphery 0.0004 0.015 0.0015 0.0004 0.0008 0.0034 0.0277 0.0021 0.0012 0.0001 0.0025 0.0077 0.0076 0.0013 0.0027 0.0005 0.0028 0.0016
Nucleolus 0.0336 0.0253 0.0241 0.0773 0.1138 0.0946 0.0653 0.0125 0.006 0.0249 0.1462 0.0551 0.0283 0.0145 0.0207 0.0256 0.1208 0.0659
Nucleus 0.955 0.9586 0.9532 0.9174 0.8817 0.8961 0.7081 0.9777 0.9652 0.9565 0.6545 0.9148 0.9382 0.9837 0.9617 0.9699 0.8004 0.9182
Peroxisomes 0.0003 0 0.0005 0 0 0 0.0098 0.0001 0.0003 0.0002 0.0156 0.0023 0.0071 0 0.0002 0 0.007 0.0007
Punctate Nuclear 0.0008 0.0002 0.0023 0.0001 0.0002 0.0012 0.0266 0.0008 0.0002 0.0001 0.0016 0.0032 0.0041 0 0.0019 0 0.0081 0.0009
Vacuole 0.0007 0.0002 0.001 0.0014 0.0007 0.0006 0.006 0.0004 0.0039 0.0092 0.0125 0.0003 0.0009 0.0001 0.001 0.0012 0.0006 0.0007
Vacuole Periphery 0.0004 0.0003 0.0006 0.0015 0.0008 0.0006 0.0057 0.0004 0.0008 0.0023 0.0175 0.0004 0.0009 0.0002 0.001 0.0013 0.0012 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 69.0184 90.7562 88.0211 67.6876 73.3203 89.8968 61.7793 66.552 63.0985 68.8894
Translational Efficiency 1.2736 1.2325 1.0894 1.6612 1.3069 0.8515 1.338 1.2901 1.2492 1.2309

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nuclear protein that inhibits replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Ett1

Ett1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ett1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available