Standard name
Human Ortholog
Description Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.71 0.75 0.82 0.83 0.86 0.83 0.67 0.63 0.62 0.62 0.58 0.57 0.95 0.98 0.99 0.91 0.95 0.95 0.6 0.59 0.71 0.28 0.37 0.56
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.08 0.2 0.31 0.26 0.24 0.3 0.36 0.37 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.19 0.17 0.14 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.22 0.23 0.11 0.53 0.33 0.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.18 0.11 0.18 0.11 0.08 0.11 0.24 0.22 0.13 0.2 0.12 0.06 0 0 0.12 0.07 0.06 0.07 0.07 0.07 0.07 0.11 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 2 1 0 0 1
Bud 1 0 0 1 1 2 6 2 3 7 3 2 1 0 0 0 0 0 6 8 3 7 17 21
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 3 2 1 3 5 4 13 9 9 9 0 4 2 21 15 14 0 0 0 0 0 0
Cytoplasm 209 170 211 163 230 217 287 242 218 244 196 219 279 218 324 429 396 230 195 144 191 52 114 205
Endoplasmic Reticulum 2 2 0 0 2 2 1 2 8 11 0 7 0 0 0 13 10 4 1 1 0 1 2 1
Endosome 0 0 1 0 2 0 1 4 0 0 0 1 1 0 1 0 0 0 3 7 4 2 10 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 1 1 0 0 0 0 0
Mitochondria 9 4 1 12 21 51 134 101 86 120 121 141 0 0 0 0 0 1 2 0 2 1 2 3
Nucleus 56 38 35 3 6 3 10 8 5 14 3 14 2 0 0 16 4 3 70 57 30 100 102 74
Nuclear Periphery 0 0 1 0 0 1 1 0 2 1 1 1 0 0 0 0 0 0 0 0 0 1 2 2
Nucleolus 1 0 0 1 0 0 3 0 0 0 0 1 0 0 0 0 0 0 1 0 4 1 5 5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 12 2 7 4 11 11
Vac/Vac Membrane 46 41 29 36 29 21 48 93 76 50 69 45 18 7 7 57 30 15 22 16 17 13 32 32
Unique Cell Count 295 228 256 197 268 260 427 387 353 396 338 384 295 223 328 470 417 243 323 245 270 188 308 368
Labelled Cell Count 326 255 281 219 292 300 496 457 411 457 403 440 301 229 335 536 459 268 323 245 270 188 308 368


Bud, Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 9.0 8.0 7.0 7.0 6.7 6.7 6.7 6.8 6.9 6.6 6.5 9.4 9.9 10.1 15.7 14.8 15.2 9.1 9.8 10.4
Std Deviation (1e-4) 1.0 1.4 1.2 0.9 1.3 1.3 1.4 1.4 1.4 1.6 1.5 1.7 1.7 1.3 1.4 3.0 3.1 3.4 1.3 1.6 1.6
Intensity Change (Log2) -0.19 -0.18 -0.26 -0.26 -0.27 -0.24 -0.22 -0.28 -0.29 0.23 0.31 0.33 0.97 0.88 0.93 0.18 0.28 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1.9 0 1.3 0 0 0 0 2.4 1.9 2.8
Cytoplasm 0.1 1.1 0.3 -4.3 -5.4 -5.5 -5.6 -6.3 -6.7 4.5 5.5 7.1 3.5 5.3 4.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.6 4.2 7.3 9.8 8.7 8.3 9.6 10.6 10.8 0 0 0 0 0 0
Nucleus -4.6 -4.9 -5.4 -5.8 -5.8 -6.0 -4.8 -6.3 -4.7 -6.1 -5.7 -6.9 -5.2 -6.9 -5.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.1 -0.2 -1.2 0 4.0 3.3 0.5 3.0 0.2 -2.2 -3.4 -4.6 0.3 -1.8 -2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8333 4.7922 4.3011 4.0184 3.806 4.3754 5.5591 6.3999 5.8165 4.6619 5.5943 5.9142 3.9427 5.0433 4.6485 4.1749 3.9586 4.4021
Actin 0.0081 0.0041 0.004 0.0009 0.0186 0.0022 0.0151 0.0011 0.0248 0.0014 0.0124 0.0069 0.0008 0.001 0.004 0.0166 0.0108 0.0014
Bud 0.0011 0.011 0.0048 0.001 0.0053 0.0011 0.0021 0.0016 0.0012 0.0003 0.0011 0.0011 0.0002 0.0005 0.001 0.0003 0.0006 0.0014
Bud Neck 0.0012 0.0017 0.0012 0.0008 0.0017 0.0054 0.0064 0.0007 0.0059 0.0019 0.0028 0.0065 0.0009 0.0039 0.0012 0.0007 0.0027 0.0055
Bud Periphery 0.0005 0.0024 0.001 0.0004 0.0057 0.0004 0.0024 0.0009 0.0013 0.0001 0.0016 0.0012 0.0001 0.0001 0.0004 0.0001 0.0004 0.0007
Bud Site 0.0034 0.0215 0.0018 0.0003 0.0017 0.0006 0.0056 0.003 0.0059 0.0006 0.0035 0.0008 0.0003 0.015 0.0024 0.0004 0.0023 0.0021
Cell Periphery 0.0001 0.0002 0.0001 0 0.0001 0 0.0003 0.0001 0.0002 0 0.0003 0.0001 0 0.0001 0.0001 0 0.0002 0.0001
Cytoplasm 0.5045 0.755 0.8296 0.9172 0.8194 0.5795 0.4709 0.8945 0.8128 0.8475 0.6797 0.6588 0.6453 0.8608 0.9164 0.8566 0.8424 0.691
Cytoplasmic Foci 0.0222 0.0099 0.012 0.0108 0.016 0.0115 0.0153 0.0043 0.0078 0.0167 0.0118 0.0055 0.0027 0.009 0.0045 0.0072 0.0185 0.0085
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0.0002 0 0.0001 0.0001 0 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0037 0.0032 0.0036 0.0027 0.003 0.003 0.0056 0.0028 0.004 0.0044 0.0067 0.0036 0.0055 0.0036 0.0032 0.0044 0.0103 0.0033
Endosome 0.0162 0.0062 0.0063 0.0048 0.0055 0.0086 0.0267 0.0034 0.0109 0.0083 0.0362 0.0087 0.0032 0.0032 0.003 0.0059 0.0327 0.0036
Golgi 0.0018 0.0003 0.0004 0.0002 0.005 0.0011 0.0044 0.0003 0.0037 0.0038 0.0073 0.0021 0.0001 0.0002 0.0003 0.0005 0.0069 0.0002
Lipid Particles 0.0018 0.0001 0.0002 0.0001 0.003 0.0012 0.0023 0.0001 0.0051 0.0005 0.0226 0.0017 0 0.0001 0.0001 0.0003 0.0046 0.0001
Mitochondria 0.0029 0.0009 0.0007 0.0007 0.0013 0.0009 0.0035 0.0012 0.0034 0.001 0.0146 0.0061 0.0006 0.0003 0.0004 0.001 0.0008 0.001
None 0.0359 0.0264 0.0395 0.0191 0.0393 0.0195 0.0152 0.0065 0.0119 0.0139 0.0069 0.0079 0.0151 0.01 0.0101 0.0144 0.0119 0.0092
Nuclear Periphery 0.008 0.0086 0.005 0.0044 0.0025 0.0095 0.0183 0.0042 0.0082 0.0039 0.042 0.0079 0.0073 0.0061 0.0038 0.0147 0.0082 0.007
Nucleolus 0.0009 0.0007 0.0003 0.0005 0.0004 0.0014 0.0014 0.0003 0.0006 0.0001 0.0032 0.0009 0.0004 0.0002 0.0002 0.0002 0.0007 0.0006
Nucleus 0.3702 0.1379 0.0795 0.0236 0.0302 0.3306 0.388 0.0706 0.0808 0.0822 0.1168 0.2695 0.3126 0.0808 0.04 0.0689 0.0244 0.2479
Peroxisomes 0.0064 0.0005 0.0023 0.0003 0.0277 0.0016 0.0018 0.0003 0.0032 0.0101 0.005 0.0019 0.0001 0.0002 0.0002 0.0003 0.0015 0.0003
Punctate Nuclear 0.0078 0.0054 0.0052 0.0094 0.0124 0.0188 0.0094 0.002 0.0055 0.0021 0.0151 0.0054 0.0024 0.0025 0.0074 0.0049 0.0149 0.0137
Vacuole 0.0029 0.0034 0.002 0.0022 0.0009 0.0025 0.0038 0.0017 0.0019 0.0009 0.0074 0.0026 0.0022 0.002 0.0012 0.0019 0.0046 0.002
Vacuole Periphery 0.0005 0.0006 0.0003 0.0005 0.0002 0.0005 0.0014 0.0004 0.0006 0.0002 0.0029 0.0008 0.0003 0.0003 0.0002 0.0005 0.0005 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.5789 26.5691 20.9476 26.3265 20.4717 32.4897 29.8022 26.3471 30.02 32.5903
Translational Efficiency 1.0384 1.3326 1.5395 1.1932 1.4232 1.1125 1.2672 1.3461 1.294 1.1433

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (87%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Sgt1

Sgt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sgt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available