Standard name
Human Ortholog
Description Subunit of the NuA3 histone acetyltransferase complex; this complex acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3; shares significant sequence identity with the human candidate tumor suppressor p33-ING1 in C-terminal region
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.17 0.23 0.38 0.5 0.33 0.47 – 0.18 0.26 0.15 0.15 0.27 0.32 0.34 0.26 0.16 0.14 0 0.08 0.08 0.09 0.08 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.17 0 0.05 0 0 0 0 0 0
Endosome 0.11 0.05 0.18 0.08 0.19 0 0.1 – 0 0.06 0 0 0.14 0.25 0.27 0 0.23 0 0.08 0 0 0 0.05 0.06
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.3 0.31 0.06 0.1 0.15 0.59 0.27 – 0.7 0.5 0.59 0.65 0.06 0 0 0 0.13 0.14 0 0.05 0.09 0.07 0.08 0.09
Nucleus 0.47 0.44 0.35 0.27 0.09 0.14 0.12 – 0.27 0.28 0.33 0.36 0.49 0.34 0.3 0.52 0.42 0.55 0.28 0.38 0.23 0.23 0.2 0.13
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.15 0.11 0.08 0.09 0.09 0.07
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.09 0.05 0.21 0.15 0.12 0 0.09 – 0.06 0.11 0 0.06 0.09 0.1 0.05 0.17 0.12 0.05 0.16 0.12 0.19 0.18 0.25 0.24
Vac/Vac Membrane 0.07 0.15 0 0.06 0.09 0 0.07 – 0 0.08 0 0 0.06 0.07 0.11 0.09 0.12 0.18 0.14 0.14 0.18 0.18 0.11 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 1 1 3 4
Bud 0 2 0 0 0 1 0 – 6 2 7 4 0 0 0 0 0 0 5 4 1 3 9 13
Bud Neck 0 2 4 4 2 6 4 – 1 5 10 5 1 0 2 0 0 0 3 5 2 2 8 15
Bud Site 0 0 0 0 0 1 0 – 14 5 4 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 31 51 28 37 75 55 114 – 64 57 28 29 38 63 98 6 14 3 16 37 15 23 33 49
Endoplasmic Reticulum 0 1 0 0 0 3 0 – 0 0 1 1 0 2 0 4 3 1 2 0 0 1 2 3
Endosome 32 16 22 8 29 2 25 – 7 13 7 5 20 50 79 0 21 0 40 19 6 9 20 24
Golgi 2 0 0 0 0 0 1 – 0 1 0 0 1 1 0 0 1 0 4 1 1 1 1 0
Mitochondria 88 94 8 10 22 99 65 – 247 108 113 129 8 0 2 0 12 3 20 22 17 18 34 38
Nucleus 135 136 43 26 13 23 29 – 94 61 64 71 68 68 88 12 38 12 136 184 47 58 83 55
Nuclear Periphery 0 0 0 0 0 0 2 – 0 1 1 0 0 0 0 0 0 0 2 0 1 2 1 4
Nucleolus 1 0 0 0 1 0 0 – 4 1 1 0 0 1 0 0 1 0 74 53 16 24 37 29
Peroxisomes 0 0 0 1 0 0 1 – 0 0 0 0 0 2 7 0 0 0 0 0 0 0 0 1
SpindlePole 27 14 26 15 18 7 21 – 22 24 6 11 13 19 14 4 11 1 76 59 39 47 102 100
Vac/Vac Membrane 19 46 5 6 13 5 17 – 9 17 7 7 9 13 31 2 11 4 69 68 35 47 44 54
Unique Cell Count 289 308 124 97 151 169 243 354 216 193 198 140 198 292 23 90 22 486 482 203 257 409 424
Labelled Cell Count 335 362 136 107 173 202 279 468 295 249 265 158 219 321 28 112 24 486 482 203 257 409 424


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.0 5.0 5.3 5.6 3.4 4.3 – 3.5 4.0 4.1 3.8 4.9 5.0 5.1 5.9 6.1 5.7 4.4 4.3 4.5
Std Deviation (1e-4) 0.8 0.7 1.4 5.0 4.6 0.8 1.5 – 0.9 0.9 2.9 0.6 1.3 1.7 1.6 1.6 1.6 1.4 1.7 1.5 1.5
Intensity Change (Log2) – – – 0.1 0.17 -0.55 -0.21 – -0.52 -0.32 -0.29 -0.39 -0.03 0.01 0.04 0.25 0.3 0.19 -0.18 -0.21 -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 2.2 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.1975 -1.1908 -1.4066 -1.5304 -1.2052 -1.2253 1.223 0.2607 0.2488 -0.0569 -0.3789 0.3366 2.1098 1.8073 2.7012 3.2454 2.7514 2.7834
Actin 0.0164 0.0005 0.0008 0.0008 0.0033 0.0008 0.0682 0.001 0.0075 0.0023 0.0005 0.0043 0.0003 0.0003 0.0003 0.0012 0.0003 0.0025
Bud 0.0004 0.0002 0.0024 0.0106 0.0003 0.0014 0.0006 0.0004 0.0007 0.0002 0.0001 0.0012 0.0001 0.0001 0.0001 0.0003 0 0.0005
Bud Neck 0.0029 0.001 0.0012 0.0009 0.0014 0.0037 0.0059 0.001 0.0011 0.0012 0.002 0.0109 0.0013 0.0007 0.0009 0.0014 0.0007 0.007
Bud Periphery 0.0007 0.0002 0.0006 0.0017 0.0007 0.0006 0.0012 0.0002 0.0008 0.0001 0.0001 0.0021 0.0001 0.0001 0.0001 0.0002 0 0.0006
Bud Site 0.0017 0.0016 0.0007 0.0017 0.0008 0.0003 0.0039 0.0087 0.0045 0.0004 0.0004 0.0039 0.0006 0.0016 0.0009 0.0008 0.0002 0.0009
Cell Periphery 0.0002 0.0001 0.0001 0.0003 0.0001 0 0.0004 0.0001 0.0001 0 0 0.0001 0 0.0001 0 0 0 0.0001
Cytoplasm 0.0687 0.1311 0.0946 0.0452 0.0998 0.0625 0.064 0.1283 0.1066 0.054 0.1643 0.088 0.074 0.1349 0.0944 0.0946 0.1234 0.1187
Cytoplasmic Foci 0.0207 0.0077 0.0076 0.0106 0.0176 0.0066 0.0255 0.0072 0.0104 0.0183 0.0069 0.0096 0.0054 0.0108 0.0047 0.0102 0.0083 0.0084
Eisosomes 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0006 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0058 0.0034 0.0025 0.0024 0.0039 0.0011 0.0063 0.0025 0.002 0.0007 0.0022 0.0027 0.0011 0.0036 0.0016 0.0019 0.0024 0.002
Endosome 0.0242 0.0048 0.0047 0.0118 0.0306 0.0037 0.0256 0.0034 0.0042 0.0064 0.0036 0.0098 0.0026 0.003 0.004 0.0047 0.0033 0.0042
Golgi 0.0033 0.0002 0.0001 0.0009 0.0179 0.0004 0.0063 0.0002 0.0012 0.0048 0.0001 0.0021 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002
Lipid Particles 0.0114 0.0042 0.001 0.0041 0.0127 0.0031 0.0106 0.0005 0.0018 0.0287 0.0004 0.0023 0.0003 0.0005 0.0003 0.0001 0.0003 0.0009
Mitochondria 0.0056 0.0007 0.0013 0.0028 0.005 0.0043 0.002 0.0008 0.0018 0.0225 0.0013 0.0024 0.0005 0.0006 0.0008 0.0014 0.0004 0.0007
None 0.1763 0.2497 0.1985 0.1399 0.1986 0.1333 0.1356 0.2704 0.2478 0.0783 0.2687 0.1682 0.1743 0.2377 0.2469 0.305 0.472 0.2149
Nuclear Periphery 0.0488 0.0207 0.0214 0.0312 0.0404 0.0131 0.031 0.0134 0.0147 0.0085 0.0166 0.0175 0.0069 0.0141 0.01 0.018 0.0157 0.0091
Nucleolus 0.0091 0.0104 0.0126 0.0136 0.016 0.0174 0.0066 0.0074 0.0079 0.0048 0.0113 0.0107 0.0097 0.0066 0.0104 0.0079 0.0083 0.0082
Nucleus 0.48 0.4795 0.5505 0.597 0.3726 0.658 0.4131 0.4522 0.5114 0.6321 0.4171 0.5676 0.5674 0.5241 0.4983 0.3825 0.2838 0.5254
Peroxisomes 0.003 0.0005 0.0003 0.0009 0.0022 0.0008 0.0056 0.0004 0.0023 0.0384 0.0003 0.0007 0.0002 0.0001 0.0002 0.0004 0.0001 0.0006
Punctate Nuclear 0.1127 0.0801 0.0968 0.1183 0.1633 0.0875 0.1833 0.0994 0.0708 0.0969 0.1024 0.0926 0.1537 0.0578 0.1239 0.1674 0.0791 0.0939
Vacuole 0.0049 0.0029 0.0017 0.0037 0.0088 0.001 0.0028 0.0024 0.002 0.0008 0.0012 0.0022 0.0012 0.0028 0.0014 0.0011 0.001 0.0011
Vacuole Periphery 0.0029 0.0005 0.0005 0.0015 0.0039 0.0004 0.0011 0.0003 0.0004 0.0005 0.0003 0.0009 0.0002 0.0004 0.0004 0.0004 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.8514 8.9671 5.545 9.3123 6.5028 6.6836 6.4222 9.6889 9.0503 9.1549
Translational Efficiency 0.8838 0.4879 0.8478 0.7316 0.6726 0.7595 0.9286 0.7404 0.7192 0.2593

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the NuA3 histone acetyltransferase complex; this complex acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3; shares significant sequence identity with the human candidate tumor suppressor p33-ING1 in C-terminal region
Localization
Cell Percentages nucleus (83%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Yng1

Yng1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yng1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available