Standard name | |
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Human Ortholog | |
Description | Cytochrome c1; component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 3 | 0 | 1 | 0 | 1 | 0 | 3 | – | 1 | 0 | 0 | 2 | 1 | 3 | 2 | 3 | 5 | 6 | 29 | 6 | 8 | 7 | 14 |
Bud | 0 | 5 | 8 | 2 | 6 | 14 | 14 | 13 | – | 5 | 10 | 17 | 1 | 2 | 2 | 0 | 4 | 2 | 0 | 1 | 0 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 6 | 5 | 2 | – | 6 | 6 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 15 | 3 | 2 | 3 | 0 | 1 | 1 | – | 1 | 1 | 0 | 3 | 3 | 7 | 5 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 3 | 4 | 1 | 2 | 6 | 6 | 4 | – | 3 | 2 | 6 | 3 | 3 | 1 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 1 | 11 | 5 | 4 | – | 4 | 6 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1 | 1 | 6 | 3 | 7 | 0 | 3 | 4 | – | 2 | 0 | 1 | 6 | 14 | 19 | 0 | 1 | 1 | 0 | 12 | 10 | 5 | 6 | 5 |
Golgi | 3 | 15 | 0 | 2 | 1 | 2 | 0 | 0 | – | 0 | 2 | 1 | 29 | 37 | 51 | 8 | 19 | 11 | 5 | 38 | 16 | 14 | 25 | 26 |
Mitochondria | 47 | 316 | 250 | 151 | 219 | 318 | 426 | 525 | – | 410 | 334 | 341 | 107 | 106 | 136 | 0 | 4 | 6 | 48 | 253 | 192 | 86 | 99 | 106 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 4 | – | 4 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 10 | 11 | 3 | 10 | 24 | 44 | 46 | – | 37 | 28 | 31 | 2 | 3 | 3 | 1 | 3 | 3 | 1 | 1 | 0 | 1 | 2 | 2 |
Nucleolus | 8 | 5 | 14 | 5 | 18 | 44 | 65 | 88 | – | 54 | 42 | 52 | 5 | 2 | 5 | 0 | 3 | 1 | 1 | 0 | 0 | 1 | 0 | 1 |
Peroxisomes | 0 | 20 | 55 | 7 | 8 | 4 | 19 | 15 | – | 12 | 5 | 5 | 15 | 6 | 18 | 2 | 13 | 3 | 1 | 20 | 53 | 21 | 27 | 22 |
SpindlePole | 2 | 1 | 4 | 2 | 0 | 1 | 8 | 6 | – | 9 | 3 | 1 | 2 | 1 | 7 | 0 | 3 | 0 | 2 | 0 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 5 | 30 | 4 | 4 | 22 | 7 | 7 | 14 | – | 10 | 5 | 6 | 14 | 17 | 38 | 2 | 8 | 9 | 0 | 3 | 0 | 1 | 1 | 7 |
Unique Cell Count | 64 | 343 | 273 | 158 | 243 | 366 | 483 | 605 | 453 | 361 | 373 | 137 | 143 | 208 | 17 | 58 | 38 | 74 | 370 | 285 | 144 | 175 | 195 | |
Labelled Cell Count | 74 | 426 | 359 | 183 | 297 | 441 | 606 | 729 | 558 | 444 | 465 | 190 | 198 | 292 | 21 | 67 | 42 | 74 | 370 | 285 | 144 | 175 | 195 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 18.4 | 14.4 | 9.6 | 9.8 | 9.3 | 9.3 | 9.1 | 10.0 | – | 10.3 | 11.2 | 11.6 | 10.6 | 10.6 | 10.2 | 14.7 | 14.0 | 14.6 | 12.3 | 12.9 | 13.0 |
Std Deviation (1e-4) | 8.6 | 3.9 | 2.9 | 4.6 | 3.3 | 3.6 | 3.5 | 3.8 | – | 4.3 | 4.7 | 4.5 | 2.7 | 2.8 | 4.6 | 3.7 | 3.8 | 3.9 | 3.6 | 4.4 | 3.9 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.04 | -0.04 | -0.08 | 0.07 | – | 0.1 | 0.22 | 0.27 | 0.14 | 0.14 | 0.1 | 0.62 | 0.55 | 0.61 | 0.36 | 0.43 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | -0.3 | 0.6 | 0 | -0.7 | – | 0 | -0.1 | 1.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0.5 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3.4 | 3.4 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 7.9 | 8.8 | 8.7 | 0 | 0 | 0 |
Mitochondria | 1.6 | -0.6 | -1.9 | -1.4 | -2.0 | – | -0.5 | 0.4 | -0.1 | -3.8 | -4.8 | -7.1 | -10.6 | -13.9 | -11.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.2 | 0 | 1.4 | 2.6 | 2.0 | – | 2.2 | 1.9 | 2.2 | 0 | 0 | -1.7 | 0 | 0 | 0 |
Nucleolus | -1.0 | 1.1 | 3.0 | 3.6 | 4.0 | – | 3.0 | 2.9 | 3.7 | -0.7 | -1.9 | -1.5 | 0 | 0 | 0 |
Peroxisomes | -4.5 | -5.8 | -8.2 | -7.2 | -8.9 | – | -7.9 | -8.0 | -8.1 | -2.3 | -4.4 | -3.5 | 0 | 0.4 | -1.8 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.9 | 0 | 0 | 0.8 | – | 0.7 | 0 | 0 | 4.1 | 4.6 | 6.5 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.1776 | 12.1125 | 11.6069 | 9.9511 | 10.705 | 11.1356 | 20.5656 | 18.6902 | 19.8332 | 20.0645 | 18.5859 | 18.9104 | 18.2679 | 16.5956 | 18.7008 | 16.5511 | 18.0826 | 17.5973 |
Actin | 0.0159 | 0.03 | 0.0186 | 0.008 | 0.027 | 0.0066 | 0.0121 | 0.0126 | 0.0128 | 0.0004 | 0.0015 | 0.0033 | 0.0099 | 0.0091 | 0.0034 | 0.0167 | 0.0027 | 0.0109 |
Bud | 0.0002 | 0.0011 | 0.0006 | 0.0002 | 0.0005 | 0.0008 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Periphery | 0.0004 | 0.0012 | 0.0014 | 0.0004 | 0.002 | 0.0033 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Bud Site | 0.001 | 0.0033 | 0.0076 | 0.0009 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0013 | 0.0002 | 0.0015 | 0.0006 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0133 | 0.0088 | 0.0065 | 0.0106 | 0.0109 | 0.0017 | 0.0016 | 0.0023 | 0.001 | 0.0001 | 0.0011 | 0.0003 | 0.001 | 0.0025 | 0.0011 | 0.0006 | 0.0001 | 0.001 |
Eisosomes | 0.0005 | 0.0006 | 0.0006 | 0.0004 | 0.0003 | 0.0004 | 0.0009 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0005 | 0.0009 | 0.0003 | 0.0004 | 0.0006 | 0.001 | 0.0005 |
Endoplasmic Reticulum | 0.0019 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0201 | 0.0166 | 0.0075 | 0.026 | 0.0159 | 0.0038 | 0.0017 | 0.006 | 0.0012 | 0.0001 | 0.0026 | 0.0014 | 0.0026 | 0.0078 | 0.0028 | 0.0036 | 0.0011 | 0.0028 |
Golgi | 0.043 | 0.0709 | 0.0557 | 0.1123 | 0.0668 | 0.0315 | 0.0139 | 0.0446 | 0.0069 | 0.0012 | 0.0227 | 0.0224 | 0.0209 | 0.0405 | 0.0237 | 0.0191 | 0.016 | 0.0332 |
Lipid Particles | 0.0325 | 0.0135 | 0.0167 | 0.019 | 0.0272 | 0.0144 | 0.0142 | 0.0053 | 0.0038 | 0.0004 | 0.0202 | 0.0014 | 0.0161 | 0.0199 | 0.016 | 0.0027 | 0.0004 | 0.0126 |
Mitochondria | 0.7951 | 0.8174 | 0.8498 | 0.7827 | 0.8166 | 0.9144 | 0.9346 | 0.8913 | 0.9628 | 0.9925 | 0.9367 | 0.9658 | 0.9328 | 0.9008 | 0.9435 | 0.9228 | 0.9761 | 0.9212 |
None | 0.0005 | 0 | 0.0002 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0077 | 0.0001 | 0.0034 | 0.0003 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.0045 | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0015 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.0433 | 0.0285 | 0.0227 | 0.0311 | 0.018 | 0.0198 | 0.014 | 0.0333 | 0.0079 | 0.005 | 0.0126 | 0.0041 | 0.0096 | 0.0134 | 0.004 | 0.0307 | 0.0017 | 0.0099 |
Punctate Nuclear | 0.0043 | 0 | 0.0002 | 0.0005 | 0.0002 | 0 | 0.0001 | 0 | 0.0011 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0045 | 0.0018 | 0.001 | 0.0015 | 0.0027 | 0.0006 | 0.0011 | 0.0006 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0009 | 0.0005 | 0.0006 | 0.0003 | 0.0001 | 0.0005 |
Vacuole Periphery | 0.0079 | 0.0049 | 0.0044 | 0.0044 | 0.0091 | 0.0023 | 0.0053 | 0.0035 | 0.0015 | 0.0001 | 0.0018 | 0.0006 | 0.0043 | 0.0048 | 0.0041 | 0.0028 | 0.0009 | 0.007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.3224 | 14.8487 | 20.4627 | 41.8909 | 26.151 | 29.645 | 26.0055 | 26.7803 | 33.4365 | 22.2665 |
Translational Efficiency | 1.2112 | 1.6758 | 1.8003 | 1.3819 | 1.4049 | 1.054 | 1.053 | 1.6765 | 1.4155 | 1.2344 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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