Standard name | |
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Human Ortholog | |
Description | Thymidylate synthase; required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S; human homolog TYMSOS can complement yeast cdc21 temperature-sensitive mutant at restrictive temperature |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 0 | 2 | 3 |
Bud | 0 | 2 | 1 | 2 | 3 | 13 | 10 | 13 | 19 | 23 | 18 | 19 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 8 | 0 | 1 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 1 | 0 | 1 | 0 | 2 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Cytoplasm | 203 | 341 | 518 | 487 | 672 | 821 | 1078 | 964 | 844 | 877 | 702 | 877 | 149 | 165 | 195 | 217 | 169 | 134 | 80 | 184 | 148 | 40 | 116 | 111 |
Endoplasmic Reticulum | 3 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 2 | 1 | 0 | 1 | 3 | 1 | 19 | 10 | 5 | 1 | 0 | 9 | 1 | 3 | 4 |
Endosome | 0 | 0 | 0 | 2 | 4 | 3 | 3 | 3 | 3 | 2 | 5 | 15 | 0 | 0 | 0 | 3 | 3 | 0 | 2 | 4 | 12 | 0 | 2 | 5 |
Golgi | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 0 |
Mitochondria | 6 | 16 | 22 | 9 | 35 | 149 | 252 | 186 | 222 | 305 | 268 | 240 | 0 | 1 | 0 | 6 | 5 | 2 | 6 | 5 | 17 | 5 | 14 | 16 |
Nucleus | 1 | 0 | 0 | 1 | 1 | 6 | 9 | 14 | 3 | 1 | 1 | 5 | 1 | 1 | 0 | 14 | 10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 2 | 0 | 5 | 5 | 13 | 8 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 3 | 5 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 2 | 0 | 1 | 4 |
Vac/Vac Membrane | 16 | 7 | 24 | 3 | 64 | 54 | 38 | 50 | 57 | 66 | 55 | 132 | 0 | 0 | 0 | 74 | 68 | 46 | 125 | 138 | 315 | 66 | 107 | 108 |
Unique Cell Count | 215 | 354 | 541 | 493 | 711 | 913 | 1219 | 1095 | 974 | 1052 | 858 | 1100 | 150 | 168 | 196 | 281 | 224 | 175 | 222 | 352 | 540 | 119 | 257 | 271 |
Labelled Cell Count | 231 | 368 | 571 | 505 | 786 | 1054 | 1411 | 1248 | 1160 | 1285 | 1056 | 1294 | 153 | 175 | 197 | 337 | 266 | 191 | 222 | 352 | 540 | 119 | 257 | 271 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 3.5 | 3.6 | 3.6 | 3.9 | 3.2 | 3.2 | 3.6 | 3.2 | 3.2 | 3.1 | 3.3 | 4.4 | 4.4 | 4.8 | 5.1 | 5.1 | 5.1 | 4.4 | 4.7 | 4.9 |
Std Deviation (1e-4) | 0.5 | 0.5 | 1.0 | 1.4 | 1.4 | 1.3 | 1.0 | 1.3 | 1.2 | 0.8 | 0.9 | 0.7 | 0.6 | 0.7 | 1.0 | 0.8 | 1.0 | 1.0 | 0.9 | 1.1 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.12 | -0.19 | -0.17 | 0.02 | -0.19 | -0.16 | -0.21 | -0.13 | 0.29 | 0.3 | 0.41 | 0.52 | 0.51 | 0.52 | 0.28 | 0.38 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 9.2 | 10.0 | 8.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.9606 | -0.7066 | -0.7266 | -1.0161 | -1.1234 | -0.8881 | 1.1835 | 1.3521 | 1.3279 | 1.592 | 1.4134 | 1.2635 | -0.5393 | 0.2761 | -0.332 | -0.3796 | 0.0918 | -0.1388 |
Actin | 0.0291 | 0.001 | 0.0255 | 0.0239 | 0.0188 | 0.0071 | 0.0375 | 0.0008 | 0.0178 | 0.0178 | 0.0313 | 0.0099 | 0.0025 | 0.0005 | 0.0146 | 0 | 0.0032 | 0.0001 |
Bud | 0.0008 | 0.0006 | 0.0008 | 0.0005 | 0.0009 | 0.0005 | 0.0022 | 0.0113 | 0.0029 | 0.0007 | 0.0013 | 0.0033 | 0.0003 | 0.0009 | 0.0005 | 0.0002 | 0.0009 | 0.0002 |
Bud Neck | 0.0024 | 0.0043 | 0.0011 | 0.0005 | 0.0011 | 0.0032 | 0.0023 | 0.0003 | 0.0036 | 0.0006 | 0.002 | 0.0025 | 0.0004 | 0.0055 | 0.0003 | 0.0006 | 0.0004 | 0.001 |
Bud Periphery | 0.0015 | 0.0011 | 0.0013 | 0.0011 | 0.0012 | 0.0012 | 0.0052 | 0.026 | 0.0048 | 0.001 | 0.0018 | 0.0071 | 0.0005 | 0.001 | 0.0004 | 0.0003 | 0.002 | 0.0003 |
Bud Site | 0.0023 | 0.0082 | 0.0059 | 0.0007 | 0.0032 | 0.0004 | 0.0062 | 0.0068 | 0.0136 | 0.0013 | 0.0026 | 0.001 | 0.0014 | 0.011 | 0.001 | 0.0004 | 0.0021 | 0.0002 |
Cell Periphery | 0.0006 | 0.0005 | 0.0004 | 0.0003 | 0.0004 | 0.0005 | 0.0008 | 0.001 | 0.0009 | 0.0004 | 0.0004 | 0.0004 | 0.0007 | 0.0009 | 0.0004 | 0.0002 | 0.0019 | 0.0003 |
Cytoplasm | 0.0774 | 0.1293 | 0.0997 | 0.1401 | 0.2021 | 0.1896 | 0.1386 | 0.1903 | 0.1501 | 0.1114 | 0.213 | 0.3078 | 0.2117 | 0.2068 | 0.1953 | 0.1779 | 0.0875 | 0.3216 |
Cytoplasmic Foci | 0.0486 | 0.048 | 0.037 | 0.042 | 0.0367 | 0.0583 | 0.0516 | 0.0442 | 0.0437 | 0.104 | 0.08 | 0.0621 | 0.0314 | 0.0213 | 0.0395 | 0.1329 | 0.0342 | 0.0397 |
Eisosomes | 0.0006 | 0.0005 | 0.0007 | 0.0008 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0005 | 0 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0044 | 0.0025 | 0.0024 | 0.0022 | 0.0036 | 0.0027 | 0.0062 | 0.0028 | 0.003 | 0.0033 | 0.0036 | 0.0023 | 0.0105 | 0.0054 | 0.0065 | 0.0022 | 0.0633 | 0.0082 |
Endosome | 0.0475 | 0.0472 | 0.0526 | 0.1136 | 0.0767 | 0.0525 | 0.0536 | 0.0325 | 0.057 | 0.13 | 0.125 | 0.0578 | 0.0861 | 0.0309 | 0.1146 | 0.1914 | 0.2282 | 0.1467 |
Golgi | 0.0101 | 0.0014 | 0.0042 | 0.0025 | 0.0037 | 0.0025 | 0.0099 | 0.0008 | 0.0066 | 0.0115 | 0.0266 | 0.0032 | 0.0059 | 0.0007 | 0.0022 | 0.0047 | 0.0071 | 0.0019 |
Lipid Particles | 0.0181 | 0.0133 | 0.0128 | 0.0146 | 0.0045 | 0.008 | 0.0134 | 0.003 | 0.0065 | 0.0181 | 0.04 | 0.0037 | 0.0106 | 0.0035 | 0.007 | 0.0113 | 0.0051 | 0.0028 |
Mitochondria | 0.0098 | 0.0068 | 0.0061 | 0.0219 | 0.0016 | 0.0024 | 0.0176 | 0.002 | 0.0057 | 0.0043 | 0.0205 | 0.0054 | 0.0188 | 0.0012 | 0.0044 | 0.0066 | 0.0349 | 0.0012 |
None | 0.6888 | 0.6363 | 0.6747 | 0.5293 | 0.4569 | 0.5726 | 0.5548 | 0.6214 | 0.6201 | 0.4798 | 0.3159 | 0.4165 | 0.4743 | 0.6102 | 0.4932 | 0.3082 | 0.3439 | 0.2018 |
Nuclear Periphery | 0.0065 | 0.0021 | 0.0083 | 0.0041 | 0.0043 | 0.0018 | 0.013 | 0.0024 | 0.0017 | 0.0024 | 0.0036 | 0.0015 | 0.0052 | 0.0032 | 0.0058 | 0.0025 | 0.0118 | 0.0061 |
Nucleolus | 0.0011 | 0.0021 | 0.0019 | 0.0053 | 0.0012 | 0.0035 | 0.0014 | 0.0018 | 0.0015 | 0.0024 | 0.0012 | 0.0013 | 0.0026 | 0.0015 | 0.0011 | 0.0015 | 0.0004 | 0.0007 |
Nucleus | 0.0032 | 0.0057 | 0.0043 | 0.0091 | 0.0067 | 0.007 | 0.0035 | 0.0038 | 0.003 | 0.003 | 0.0048 | 0.0072 | 0.0036 | 0.0034 | 0.004 | 0.0035 | 0.0028 | 0.0187 |
Peroxisomes | 0.0132 | 0.0026 | 0.0049 | 0.0078 | 0.0128 | 0.0096 | 0.0174 | 0.0006 | 0.004 | 0.0455 | 0.0127 | 0.0061 | 0.0031 | 0.0005 | 0.0008 | 0.0017 | 0.0004 | 0.0003 |
Punctate Nuclear | 0.0036 | 0.0019 | 0.0027 | 0.0037 | 0.003 | 0.0055 | 0.0057 | 0.0027 | 0.0021 | 0.0148 | 0.0042 | 0.0018 | 0.0089 | 0.0011 | 0.0009 | 0.0016 | 0.0004 | 0.001 |
Vacuole | 0.0269 | 0.0776 | 0.0463 | 0.0694 | 0.1501 | 0.066 | 0.0522 | 0.0428 | 0.0464 | 0.0422 | 0.1014 | 0.0917 | 0.1156 | 0.0854 | 0.0969 | 0.1412 | 0.1479 | 0.228 |
Vacuole Periphery | 0.0033 | 0.0073 | 0.0063 | 0.0066 | 0.0107 | 0.0051 | 0.0064 | 0.0027 | 0.0046 | 0.0052 | 0.0077 | 0.0073 | 0.0057 | 0.0046 | 0.01 | 0.011 | 0.0213 | 0.0193 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 86.8253 | 36.6522 | 3.9996 | 23.5098 | 87.5566 | 54.2358 | 26.7043 | 18.0236 | 21.6964 | 68.664 |
Translational Efficiency | 2.2661 | 1.6225 | 3.3875 | 1.5176 | 1.2193 | 3.6721 | 1.7678 | 0.9749 | 1.3292 | 1.7173 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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