Standard name
Human Ortholog
Description Thymidylate synthase; required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S; human homolog TYMSOS can complement yeast cdc21 temperature-sensitive mutant at restrictive temperature

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.96 0.96 0.99 0.95 0.9 0.88 0.88 0.87 0.83 0.82 0.8 0.99 0.98 0.99 0.77 0.75 0.77 0.36 0.53 0.27 0.34 0.45 0.41
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0.05 0.16 0.21 0.17 0.23 0.29 0.31 0.22 0 0 0 0 0 0 0 0 0 0.05 0.05 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0 0 0.09 0.06 0 0.05 0.06 0.06 0.06 0.12 0 0 0 0.26 0.3 0.26 0.57 0.39 0.58 0.56 0.42 0.4
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 1 0 0 0 1 0 0 0 1 0 0 0 1 3 0 2 3
Bud 0 2 1 2 3 13 10 13 19 23 18 19 0 0 0 1 0 0 1 2 8 0 1 6
Bud Neck 0 0 0 0 0 0 1 4 1 0 1 1 0 1 0 0 0 0 0 4 4 0 1 2
Bud Site 0 0 0 0 0 0 0 0 2 2 0 0 0 0 0 0 0 0
Cell Periphery 1 0 0 1 0 1 3 0 1 0 1 0 2 4 1 1 0 0 0 0 0 0 0 2
Cytoplasm 203 341 518 487 672 821 1078 964 844 877 702 877 149 165 195 217 169 134 80 184 148 40 116 111
Endoplasmic Reticulum 3 1 1 0 1 0 1 1 1 2 1 0 1 3 1 19 10 5 1 0 9 1 3 4
Endosome 0 0 0 2 4 3 3 3 3 2 5 15 0 0 0 3 3 0 2 4 12 0 2 5
Golgi 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 0 1 0
Mitochondria 6 16 22 9 35 149 252 186 222 305 268 240 0 1 0 6 5 2 6 5 17 5 14 16
Nucleus 1 0 0 1 1 6 9 14 3 1 1 5 1 1 0 14 10 2 0 0 0 0 0 0
Nuclear Periphery 0 0 2 0 5 5 13 8 1 2 0 0 0 0 0 1 0 0 0 0 1 0 0 0
Nucleolus 0 0 2 0 0 2 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0
SpindlePole 0 0 0 0 1 0 2 3 3 5 4 4 0 0 0 0 0 2 2 3 2 0 1 4
Vac/Vac Membrane 16 7 24 3 64 54 38 50 57 66 55 132 0 0 0 74 68 46 125 138 315 66 107 108
Unique Cell Count 215 354 541 493 711 913 1219 1095 974 1052 858 1100 150 168 196 281 224 175 222 352 540 119 257 271
Labelled Cell Count 231 368 571 505 786 1054 1411 1248 1160 1285 1056 1294 153 175 197 337 266 191 222 352 540 119 257 271


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.5 3.6 3.6 3.9 3.2 3.2 3.6 3.2 3.2 3.1 3.3 4.4 4.4 4.8 5.1 5.1 5.1 4.4 4.7 4.9
Std Deviation (1e-4) 0.5 0.5 1.0 1.4 1.4 1.3 1.0 1.3 1.2 0.8 0.9 0.7 0.6 0.7 1.0 0.8 1.0 1.0 0.9 1.1 1.4
Intensity Change (Log2) 0.01 0.12 -0.19 -0.17 0.02 -0.19 -0.16 -0.21 -0.13 0.29 0.3 0.41 0.52 0.51 0.52 0.28 0.38 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 9.2 10.0 8.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.9606 -0.7066 -0.7266 -1.0161 -1.1234 -0.8881 1.1835 1.3521 1.3279 1.592 1.4134 1.2635 -0.5393 0.2761 -0.332 -0.3796 0.0918 -0.1388
Actin 0.0291 0.001 0.0255 0.0239 0.0188 0.0071 0.0375 0.0008 0.0178 0.0178 0.0313 0.0099 0.0025 0.0005 0.0146 0 0.0032 0.0001
Bud 0.0008 0.0006 0.0008 0.0005 0.0009 0.0005 0.0022 0.0113 0.0029 0.0007 0.0013 0.0033 0.0003 0.0009 0.0005 0.0002 0.0009 0.0002
Bud Neck 0.0024 0.0043 0.0011 0.0005 0.0011 0.0032 0.0023 0.0003 0.0036 0.0006 0.002 0.0025 0.0004 0.0055 0.0003 0.0006 0.0004 0.001
Bud Periphery 0.0015 0.0011 0.0013 0.0011 0.0012 0.0012 0.0052 0.026 0.0048 0.001 0.0018 0.0071 0.0005 0.001 0.0004 0.0003 0.002 0.0003
Bud Site 0.0023 0.0082 0.0059 0.0007 0.0032 0.0004 0.0062 0.0068 0.0136 0.0013 0.0026 0.001 0.0014 0.011 0.001 0.0004 0.0021 0.0002
Cell Periphery 0.0006 0.0005 0.0004 0.0003 0.0004 0.0005 0.0008 0.001 0.0009 0.0004 0.0004 0.0004 0.0007 0.0009 0.0004 0.0002 0.0019 0.0003
Cytoplasm 0.0774 0.1293 0.0997 0.1401 0.2021 0.1896 0.1386 0.1903 0.1501 0.1114 0.213 0.3078 0.2117 0.2068 0.1953 0.1779 0.0875 0.3216
Cytoplasmic Foci 0.0486 0.048 0.037 0.042 0.0367 0.0583 0.0516 0.0442 0.0437 0.104 0.08 0.0621 0.0314 0.0213 0.0395 0.1329 0.0342 0.0397
Eisosomes 0.0006 0.0005 0.0007 0.0008 0.0001 0.0001 0.0005 0.0001 0.0003 0.0002 0.0003 0.0001 0.0001 0.0003 0.0005 0 0.0003 0
Endoplasmic Reticulum 0.0044 0.0025 0.0024 0.0022 0.0036 0.0027 0.0062 0.0028 0.003 0.0033 0.0036 0.0023 0.0105 0.0054 0.0065 0.0022 0.0633 0.0082
Endosome 0.0475 0.0472 0.0526 0.1136 0.0767 0.0525 0.0536 0.0325 0.057 0.13 0.125 0.0578 0.0861 0.0309 0.1146 0.1914 0.2282 0.1467
Golgi 0.0101 0.0014 0.0042 0.0025 0.0037 0.0025 0.0099 0.0008 0.0066 0.0115 0.0266 0.0032 0.0059 0.0007 0.0022 0.0047 0.0071 0.0019
Lipid Particles 0.0181 0.0133 0.0128 0.0146 0.0045 0.008 0.0134 0.003 0.0065 0.0181 0.04 0.0037 0.0106 0.0035 0.007 0.0113 0.0051 0.0028
Mitochondria 0.0098 0.0068 0.0061 0.0219 0.0016 0.0024 0.0176 0.002 0.0057 0.0043 0.0205 0.0054 0.0188 0.0012 0.0044 0.0066 0.0349 0.0012
None 0.6888 0.6363 0.6747 0.5293 0.4569 0.5726 0.5548 0.6214 0.6201 0.4798 0.3159 0.4165 0.4743 0.6102 0.4932 0.3082 0.3439 0.2018
Nuclear Periphery 0.0065 0.0021 0.0083 0.0041 0.0043 0.0018 0.013 0.0024 0.0017 0.0024 0.0036 0.0015 0.0052 0.0032 0.0058 0.0025 0.0118 0.0061
Nucleolus 0.0011 0.0021 0.0019 0.0053 0.0012 0.0035 0.0014 0.0018 0.0015 0.0024 0.0012 0.0013 0.0026 0.0015 0.0011 0.0015 0.0004 0.0007
Nucleus 0.0032 0.0057 0.0043 0.0091 0.0067 0.007 0.0035 0.0038 0.003 0.003 0.0048 0.0072 0.0036 0.0034 0.004 0.0035 0.0028 0.0187
Peroxisomes 0.0132 0.0026 0.0049 0.0078 0.0128 0.0096 0.0174 0.0006 0.004 0.0455 0.0127 0.0061 0.0031 0.0005 0.0008 0.0017 0.0004 0.0003
Punctate Nuclear 0.0036 0.0019 0.0027 0.0037 0.003 0.0055 0.0057 0.0027 0.0021 0.0148 0.0042 0.0018 0.0089 0.0011 0.0009 0.0016 0.0004 0.001
Vacuole 0.0269 0.0776 0.0463 0.0694 0.1501 0.066 0.0522 0.0428 0.0464 0.0422 0.1014 0.0917 0.1156 0.0854 0.0969 0.1412 0.1479 0.228
Vacuole Periphery 0.0033 0.0073 0.0063 0.0066 0.0107 0.0051 0.0064 0.0027 0.0046 0.0052 0.0077 0.0073 0.0057 0.0046 0.01 0.011 0.0213 0.0193

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 86.8253 36.6522 3.9996 23.5098 87.5566 54.2358 26.7043 18.0236 21.6964 68.664
Translational Efficiency 2.2661 1.6225 3.3875 1.5176 1.2193 3.6721 1.7678 0.9749 1.3292 1.7173

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Thymidylate synthase; required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S; human homolog TYMSOS can complement yeast cdc21 temperature-sensitive mutant at restrictive temperature
Localization
Cell Percentages vacuole (21%), cytoplasm (10%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cdc21

Cdc21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available