Standard name | |
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Human Ortholog | |
Description | GTP-binding protein that couples the Ski complex and exosome; putative pseudo-translational GTPase involved in 3'-to-5' mRNA decay pathway; interacts with both the cytoplasmic exosome and the Ski complex; eRF3-like domain targets nonstop mRNA for degradation; null mutants have a superkiller phenotype; SKI7 has a paralog, HBS1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 1 | 2 | 3 |
Bud | 0 | 0 | 1 | 1 | 0 | 3 | 3 | 2 | 4 | 9 | 7 | 1 | 2 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 7 |
Bud Neck | 0 | 0 | 4 | 5 | 7 | 3 | 4 | 15 | 3 | 4 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 8 | 6 | 5 | 5 | 5 | 1 | 3 | 5 | 2 | 2 | 4 | 2 | 1 | 14 | 5 | 5 | 0 | 1 | 1 | 1 | 1 | 1 |
Cytoplasm | 119 | 142 | 261 | 250 | 362 | 344 | 317 | 458 | 316 | 332 | 215 | 276 | 208 | 303 | 351 | 200 | 138 | 113 | 111 | 149 | 229 | 121 | 246 | 268 |
Endoplasmic Reticulum | 2 | 4 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 3 | 4 | 2 | 0 | 5 | 6 | 2 | 5 | 0 | 0 | 1 | 5 | 12 | 9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 1 | 0 | 0 | 1 | 2 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 2 |
Mitochondria | 8 | 11 | 1 | 1 | 2 | 35 | 37 | 27 | 116 | 130 | 131 | 57 | 2 | 1 | 2 | 4 | 5 | 0 | 6 | 3 | 17 | 22 | 35 | 28 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 2 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 2 | 3 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Vac/Vac Membrane | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 3 | 4 | 5 | 2 | 6 | 0 | 0 | 0 | 2 | 6 | 2 | 1 | 6 | 5 | 5 | 11 | 16 |
Unique Cell Count | 126 | 150 | 265 | 253 | 365 | 364 | 337 | 485 | 373 | 422 | 296 | 321 | 212 | 305 | 356 | 209 | 147 | 118 | 128 | 169 | 265 | 165 | 325 | 354 |
Labelled Cell Count | 129 | 161 | 276 | 266 | 379 | 394 | 371 | 512 | 455 | 497 | 364 | 355 | 218 | 307 | 361 | 231 | 159 | 130 | 128 | 169 | 265 | 165 | 325 | 354 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.6 | 3.9 | 5.3 | 4.7 | 5.1 | 4.0 | 4.2 | 4.6 | 3.9 | 3.6 | 3.6 | 4.1 | 4.4 | 4.7 | 4.8 | 7.1 | 7.0 | 6.9 | 4.4 | 4.5 | 4.9 |
Std Deviation (1e-4) | 0.7 | 0.6 | 1.0 | 1.3 | 1.1 | 1.0 | 1.0 | 1.0 | 1.4 | 0.9 | 1.0 | 1.0 | 0.7 | 1.3 | 1.0 | 1.4 | 1.3 | 1.5 | 1.1 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.06 | -0.41 | -0.32 | -0.21 | -0.44 | -0.54 | -0.57 | -0.34 | -0.26 | -0.15 | -0.14 | 0.42 | 0.41 | 0.39 | -0.27 | -0.23 | -0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.1803 | -0.4625 | -0.7251 | -0.1962 | -0.5206 | -0.4372 | -0.6888 | -0.5708 | -0.5995 | -0.8385 | -0.9779 | -0.7151 | -0.5543 | -0.0876 | -0.6699 | -0.0727 | -0.8377 | -0.4863 |
Actin | 0.0521 | 0.0006 | 0.0114 | 0.0145 | 0.1026 | 0.0013 | 0.0173 | 0.0063 | 0.0321 | 0.0041 | 0.005 | 0.0054 | 0.0326 | 0.0004 | 0.0149 | 0.1307 | 0.0047 | 0.0073 |
Bud | 0.0009 | 0.0005 | 0.0003 | 0.0005 | 0.0017 | 0.0001 | 0.0007 | 0.0028 | 0.0057 | 0.0159 | 0.0024 | 0.0017 | 0.0016 | 0.0126 | 0.0006 | 0.0062 | 0.0005 | 0.001 |
Bud Neck | 0.0033 | 0.0015 | 0.0023 | 0.0005 | 0.0053 | 0.0003 | 0.0008 | 0.0013 | 0.004 | 0.0005 | 0.0012 | 0.0142 | 0.0044 | 0.0005 | 0.0037 | 0.0099 | 0.0012 | 0.0007 |
Bud Periphery | 0.0012 | 0.0004 | 0.0004 | 0.0008 | 0.0033 | 0.0001 | 0.0013 | 0.0039 | 0.0103 | 0.0283 | 0.0026 | 0.0026 | 0.0027 | 0.0178 | 0.0017 | 0.0125 | 0.001 | 0.0013 |
Bud Site | 0.0048 | 0.0123 | 0.0128 | 0.0033 | 0.0188 | 0 | 0.0021 | 0.0115 | 0.01 | 0.0009 | 0.0043 | 0.008 | 0.0071 | 0.0131 | 0.0061 | 0.0386 | 0.002 | 0.0002 |
Cell Periphery | 0.0004 | 0.0005 | 0.0004 | 0.0005 | 0.001 | 0.0001 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0005 | 0.0004 | 0.0006 | 0.0007 | 0.0002 | 0.0001 |
Cytoplasm | 0.1294 | 0.2664 | 0.1861 | 0.2521 | 0.236 | 0.3694 | 0.2965 | 0.3598 | 0.3655 | 0.4439 | 0.4014 | 0.4319 | 0.3364 | 0.4794 | 0.3644 | 0.2368 | 0.4366 | 0.508 |
Cytoplasmic Foci | 0.0399 | 0.0122 | 0.0369 | 0.0186 | 0.0136 | 0.0285 | 0.0269 | 0.0084 | 0.0222 | 0.0543 | 0.0317 | 0.027 | 0.0262 | 0.0087 | 0.0069 | 0.0132 | 0.0225 | 0.0157 |
Eisosomes | 0.0016 | 0.0002 | 0.0008 | 0.0008 | 0.0015 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0012 | 0.0016 | 0.0002 |
Endoplasmic Reticulum | 0.0084 | 0.0034 | 0.0021 | 0.0163 | 0.0035 | 0.0019 | 0.004 | 0.0025 | 0.0038 | 0.0021 | 0.0021 | 0.0024 | 0.0044 | 0.0018 | 0.0025 | 0.0039 | 0.001 | 0.0035 |
Endosome | 0.0353 | 0.0052 | 0.0068 | 0.0528 | 0.0085 | 0.0025 | 0.0308 | 0.003 | 0.0234 | 0.0223 | 0.0238 | 0.0169 | 0.0147 | 0.0018 | 0.0028 | 0.0093 | 0.0074 | 0.0176 |
Golgi | 0.008 | 0.0005 | 0.0008 | 0.0078 | 0.0072 | 0.0003 | 0.0063 | 0.0005 | 0.0093 | 0.0183 | 0.0172 | 0.0062 | 0.0076 | 0.0002 | 0.0005 | 0.0057 | 0.0028 | 0.0045 |
Lipid Particles | 0.0204 | 0.0008 | 0.0018 | 0.0067 | 0.0066 | 0.0011 | 0.0104 | 0.0004 | 0.0061 | 0.0078 | 0.007 | 0.0032 | 0.0267 | 0.0005 | 0.0008 | 0.0023 | 0.0187 | 0.0045 |
Mitochondria | 0.0038 | 0.0004 | 0.0005 | 0.0069 | 0.0058 | 0.0004 | 0.006 | 0.0008 | 0.0102 | 0.0036 | 0.0066 | 0.0089 | 0.01 | 0.0003 | 0.0006 | 0.0051 | 0.0038 | 0.004 |
None | 0.6018 | 0.6893 | 0.7282 | 0.5588 | 0.5594 | 0.5892 | 0.5157 | 0.5914 | 0.4771 | 0.3658 | 0.4761 | 0.4604 | 0.4824 | 0.4572 | 0.5891 | 0.5037 | 0.4749 | 0.4175 |
Nuclear Periphery | 0.037 | 0.0011 | 0.0006 | 0.0278 | 0.0094 | 0.0004 | 0.03 | 0.0012 | 0.0023 | 0.0008 | 0.0017 | 0.0009 | 0.0041 | 0.0003 | 0.0007 | 0.0053 | 0.0005 | 0.0053 |
Nucleolus | 0.0019 | 0.0002 | 0.0007 | 0.0007 | 0.0005 | 0.0001 | 0.0027 | 0.0002 | 0.0006 | 0.0002 | 0.0007 | 0.0004 | 0.0012 | 0.0005 | 0.0002 | 0.0007 | 0.0019 | 0.0002 |
Nucleus | 0.0179 | 0.001 | 0.0009 | 0.0088 | 0.0011 | 0.0003 | 0.0143 | 0.0014 | 0.0018 | 0.001 | 0.0019 | 0.0022 | 0.0047 | 0.0011 | 0.001 | 0.0022 | 0.0009 | 0.001 |
Peroxisomes | 0.0073 | 0.0002 | 0.0016 | 0.0026 | 0.0075 | 0.0028 | 0.012 | 0.0007 | 0.0083 | 0.0249 | 0.0035 | 0.0026 | 0.0251 | 0.0002 | 0.0005 | 0.0067 | 0.0119 | 0.0037 |
Punctate Nuclear | 0.0182 | 0.0006 | 0.0023 | 0.0137 | 0.0042 | 0.0005 | 0.0083 | 0.0007 | 0.0024 | 0.0009 | 0.0074 | 0.0016 | 0.0028 | 0.0006 | 0.0005 | 0.0015 | 0.0037 | 0.0018 |
Vacuole | 0.004 | 0.0025 | 0.0019 | 0.0035 | 0.0016 | 0.0007 | 0.0089 | 0.0024 | 0.0035 | 0.0034 | 0.0026 | 0.0024 | 0.0039 | 0.0022 | 0.0015 | 0.0028 | 0.0018 | 0.0013 |
Vacuole Periphery | 0.0023 | 0.0003 | 0.0002 | 0.002 | 0.0009 | 0.0001 | 0.0045 | 0.0003 | 0.001 | 0.0008 | 0.0007 | 0.0006 | 0.0006 | 0.0002 | 0.0002 | 0.0011 | 0.0004 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.0801 | 8.7912 | 8.4805 | 12.2589 | 8.2879 | 6.3887 | 12.111 | 13.1986 | 9.708 | 12.9925 |
Translational Efficiency | 0.9313 | 1.0267 | 0.9579 | 0.6608 | 1.0045 | 1.3764 | 0.8239 | 0.6777 | 0.9226 | 0.716 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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