Standard name | |
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Human Ortholog | |
Description | Component of small ribosomal subunit (SSU) processosome; this complex contains U3 snoRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; originally isolated as bud-site selection mutant that displays a random budding pattern |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 5 | 4 | – | 7 | 11 | 6 | 7 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 1 | 10 | 10 |
Bud Neck | 0 | 0 | 0 | 7 | 0 | 7 | 9 | – | 4 | 12 | 5 | 9 | 0 | 10 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 5 | 5 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 10 | 3 | – | 13 | 17 | 15 | 21 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 3 | 8 | 14 | 13 | 11 | 35 | – | 16 | 22 | 4 | 12 | 24 | 44 | 61 | 6 | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 1 |
Endosome | 6 | 4 | 3 | 1 | 23 | 1 | 46 | – | 4 | 15 | 12 | 8 | 10 | 5 | 47 | 0 | 5 | 1 | 6 | 4 | 4 | 4 | 16 | 8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 2 | 0 |
Mitochondria | 6 | 7 | 1 | 2 | 6 | 85 | 20 | – | 80 | 43 | 53 | 51 | 2 | 6 | 0 | 9 | 9 | 12 | 0 | 1 | 0 | 3 | 3 | 2 |
Nucleus | 71 | 40 | 39 | 29 | 57 | 47 | 50 | – | 49 | 44 | 45 | 42 | 34 | 58 | 48 | 36 | 47 | 33 | 50 | 37 | 20 | 26 | 51 | 64 |
Nuclear Periphery | 3 | 0 | 2 | 0 | 0 | 1 | 3 | – | 2 | 1 | 3 | 1 | 2 | 1 | 1 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Nucleolus | 182 | 76 | 117 | 195 | 194 | 192 | 253 | – | 208 | 290 | 197 | 196 | 195 | 312 | 288 | 60 | 93 | 74 | 173 | 62 | 118 | 155 | 318 | 306 |
Peroxisomes | 0 | 0 | 0 | 0 | 2 | 0 | 3 | – | 1 | 3 | 0 | 1 | 1 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 6 | 2 | 36 | 119 | 86 | 70 | 192 | – | 85 | 172 | 87 | 123 | 45 | 95 | 79 | 6 | 14 | 5 | 9 | 4 | 11 | 22 | 63 | 43 |
Vac/Vac Membrane | 0 | 3 | 0 | 1 | 3 | 1 | 2 | – | 2 | 2 | 4 | 4 | 3 | 4 | 3 | 0 | 1 | 1 | 3 | 1 | 4 | 14 | 15 | 10 |
Unique Cell Count | 235 | 100 | 155 | 226 | 277 | 285 | 398 | 318 | 406 | 284 | 304 | 241 | 396 | 399 | 95 | 133 | 100 | 252 | 118 | 168 | 234 | 506 | 462 | |
Labelled Cell Count | 277 | 137 | 206 | 369 | 385 | 430 | 621 | 471 | 633 | 431 | 475 | 317 | 537 | 535 | 122 | 182 | 133 | 252 | 118 | 168 | 234 | 506 | 462 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.3 | 8.3 | 8.6 | 8.2 | 8.1 | 5.2 | 6.8 | – | 5.3 | 6.0 | 5.7 | 5.8 | 9.7 | 8.0 | 9.3 | 10.3 | 10.4 | 10.6 | 8.7 | 9.0 | 9.1 |
Std Deviation (1e-4) | 2.1 | 1.8 | 2.4 | 2.2 | 2.0 | 1.9 | 2.1 | – | 1.4 | 1.6 | 1.3 | 1.8 | 3.1 | 2.7 | 3.1 | 3.6 | 2.7 | 2.8 | 2.1 | 2.5 | 2.8 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.07 | -0.71 | -0.33 | – | -0.68 | -0.51 | -0.59 | -0.57 | 0.18 | -0.1 | 0.12 | 0.27 | 0.28 | 0.3 | 0.02 | 0.08 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 2.9 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.4 | -0.2 | -0.6 | 1.4 | – | -0.1 | 0.1 | 0 | -0.6 | 1.7 | 2.1 | 3.2 | 0.4 | -0.3 | -0.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.7 | 0 | 3.6 | – | 0 | 0 | 1.3 | 0 | 1.2 | 0 | 3.6 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 7.4 | 2.4 | – | 6.6 | 3.9 | 5.5 | 5.2 | 0 | 0 | 0 | 0 | 0 | 4.0 |
Nucleus | -3.1 | -1.1 | -2.2 | -3.6 | – | -2.6 | -4.3 | -2.4 | -3.0 | -2.8 | -2.9 | -3.8 | 2.1 | 1.9 | 1.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.7 | -1.2 | -1.8 | -2.7 | – | -2.2 | -1.0 | -1.4 | -2.4 | 1.3 | 0.8 | -0.8 | -2.1 | -1.1 | -0.3 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 5.7 | 1.7 | 0.3 | 5.4 | – | 0.8 | 4.2 | 1.7 | 3.7 | -1.1 | 0.2 | -0.9 | -3.5 | -2.8 | -3.9 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.9184 | 5.542 | 5.1092 | 4.0913 | 4.2327 | 5.0659 | 6.7522 | 7.5317 | 6.5906 | 6.0122 | 6.7012 | 6.5147 | 9.0112 | 10.1836 | 8.6571 | 9.1122 | 7.4927 | 8.7889 |
Actin | 0.0399 | 0.001 | 0.003 | 0.0015 | 0.0156 | 0.0125 | 0.0435 | 0 | 0.0051 | 0.0398 | 0.0022 | 0.0057 | 0.0048 | 0 | 0.016 | 0.0099 | 0.0012 | 0 |
Bud | 0.0007 | 0.0002 | 0.0007 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0 |
Bud Neck | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0007 | 0.0003 | 0.0006 | 0.0002 | 0.0007 | 0.0011 | 0.0005 | 0.0012 | 0.0003 | 0.0005 | 0.0011 | 0.0038 | 0.0003 | 0.0003 |
Bud Periphery | 0.0009 | 0.0003 | 0.0013 | 0.0002 | 0.0005 | 0.0005 | 0.0004 | 0.0002 | 0.0002 | 0.0011 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0.0001 |
Bud Site | 0.0058 | 0.0024 | 0.0026 | 0.0003 | 0.002 | 0.0002 | 0.0012 | 0.001 | 0.0008 | 0.0041 | 0.0008 | 0.0007 | 0.0005 | 0.0004 | 0.0004 | 0.0024 | 0.0003 | 0.0001 |
Cell Periphery | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0007 | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.0001 |
Cytoplasm | 0.0009 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0004 | 0.0048 | 0 | 0.0026 | 0.0001 | 0.0039 | 0.0062 | 0.0047 | 0 | 0.0002 | 0.0005 | 0.0002 | 0 |
Cytoplasmic Foci | 0.0165 | 0.0015 | 0.0026 | 0.0139 | 0.0313 | 0.0015 | 0.0122 | 0 | 0.0038 | 0.0002 | 0.0083 | 0.0052 | 0.0038 | 0.0002 | 0.001 | 0.0276 | 0.0175 | 0.0001 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0024 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0014 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0008 | 0.003 | 0 | 0.0027 | 0.0004 | 0.0025 | 0.0042 | 0.0021 | 0 | 0.0006 | 0.0001 | 0 | 0 |
Endosome | 0.0243 | 0.0047 | 0.0084 | 0.0049 | 0.0302 | 0.0077 | 0.02 | 0 | 0.0029 | 0.0003 | 0.0141 | 0.0085 | 0.013 | 0.0001 | 0.0028 | 0.0024 | 0.0014 | 0 |
Golgi | 0.0284 | 0.0038 | 0.0014 | 0.009 | 0.0657 | 0.0019 | 0.0073 | 0 | 0.0018 | 0.0004 | 0.0022 | 0.0046 | 0.0011 | 0 | 0.0019 | 0.0025 | 0.0011 | 0 |
Lipid Particles | 0.0218 | 0.0007 | 0.0067 | 0.0125 | 0.0244 | 0.0037 | 0.0158 | 0.0001 | 0.0077 | 0.0004 | 0.0412 | 0.0084 | 0.0029 | 0.0005 | 0.0014 | 0.0046 | 0.0049 | 0.0004 |
Mitochondria | 0.0072 | 0.0052 | 0.0159 | 0.0013 | 0.0308 | 0.0023 | 0.005 | 0 | 0.0014 | 0.0043 | 0.0039 | 0.0015 | 0.0009 | 0.0002 | 0.0003 | 0.0088 | 0.0001 | 0.0001 |
None | 0.0016 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0006 | 0.0062 | 0 | 0.0023 | 0.0004 | 0.0021 | 0.0011 | 0.007 | 0 | 0.0004 | 0.0009 | 0.0002 | 0 |
Nuclear Periphery | 0.003 | 0.0001 | 0.0011 | 0.0001 | 0.0002 | 0.0071 | 0.0227 | 0 | 0.0018 | 0.0003 | 0.0167 | 0.0066 | 0.0132 | 0 | 0.0008 | 0.0002 | 0.0007 | 0 |
Nucleolus | 0.7556 | 0.8263 | 0.8685 | 0.8131 | 0.6664 | 0.8579 | 0.7486 | 0.8453 | 0.8386 | 0.8336 | 0.7349 | 0.8263 | 0.8327 | 0.8506 | 0.8793 | 0.8026 | 0.7686 | 0.9113 |
Nucleus | 0.0785 | 0.1507 | 0.081 | 0.1292 | 0.1055 | 0.0912 | 0.084 | 0.1523 | 0.1188 | 0.095 | 0.1141 | 0.1066 | 0.0966 | 0.1443 | 0.0906 | 0.1038 | 0.1174 | 0.0825 |
Peroxisomes | 0.0083 | 0.0003 | 0.0028 | 0.0116 | 0.0217 | 0.0006 | 0.0055 | 0 | 0.0027 | 0.0003 | 0.0026 | 0.0006 | 0.0006 | 0 | 0.0005 | 0.0211 | 0.0086 | 0 |
Punctate Nuclear | 0.0022 | 0.001 | 0.0019 | 0.0011 | 0.0009 | 0.0023 | 0.0156 | 0.0004 | 0.0046 | 0.0137 | 0.0461 | 0.0102 | 0.0138 | 0.0009 | 0.0015 | 0.0049 | 0.0769 | 0.0049 |
Vacuole | 0.0011 | 0.0006 | 0.0007 | 0.0005 | 0.0011 | 0.0039 | 0.0015 | 0.0001 | 0.0008 | 0.001 | 0.0014 | 0.0011 | 0.0008 | 0.0016 | 0.0006 | 0.0018 | 0.0002 | 0.0001 |
Vacuole Periphery | 0.0011 | 0.0002 | 0.0004 | 0.0002 | 0.0012 | 0.0044 | 0.0016 | 0 | 0.0003 | 0.0002 | 0.0013 | 0.0008 | 0.0008 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 53.0318 | 39.7609 | 73.7608 | 46.5686 | 58.7771 | 42.7439 | 42.9081 | 38.6135 | 38.6776 | 54.2969 |
Translational Efficiency | 2.2114 | 2.8216 | 1.207 | 1.6367 | 1.6923 | 1.7424 | 1.5433 | 1.2873 | 1.1553 | 1.2611 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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