Standard name
Human Ortholog
Description Component of small ribosomal subunit (SSU) processosome; this complex contains U3 snoRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; originally isolated as bud-site selection mutant that displays a random budding pattern

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.06 0.05 0 0.09 0.05 0.05 0 0 0.1 0.11 0.15 0.06 0.05 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.08 0 0.12 0 0 0 0 0 0 0.12 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0 0 0 0.3 0.05 0.25 0.11 0.19 0.17 0 0 0 0.09 0.07 0.12 0 0 0 0 0 0
Nucleus 0.3 0.4 0.25 0.13 0.21 0.16 0.13 0.15 0.11 0.16 0.14 0.14 0.15 0.12 0.38 0.35 0.33 0.2 0.32 0.12 0.11 0.1 0.14
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.77 0.76 0.75 0.86 0.7 0.67 0.64 0.65 0.71 0.69 0.64 0.81 0.79 0.72 0.63 0.7 0.74 0.69 0.53 0.7 0.67 0.63 0.66
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.23 0.53 0.31 0.25 0.48 0.27 0.42 0.31 0.4 0.19 0.24 0.2 0.06 0.11 0.05 0 0 0.07 0.1 0.13 0.09
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0
Bud 0 1 0 0 0 5 4 7 11 6 7 0 1 0 0 1 0 2 1 1 1 10 10
Bud Neck 0 0 0 7 0 7 9 4 12 5 9 0 10 0 0 2 0 0 0 0 0 5 5
Bud Site 0 0 0 1 1 10 3 13 17 15 21 0 0 1 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 3 8 14 13 11 35 16 22 4 12 24 44 61 6 6 5 0 0 0 0 0 1
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 0 1 0 0 3 0 2 0 0 0 1 2 1
Endosome 6 4 3 1 23 1 46 4 15 12 8 10 5 47 0 5 1 6 4 4 4 16 8
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 0 2 1 0 0 2 0 1 1 2 0
Mitochondria 6 7 1 2 6 85 20 80 43 53 51 2 6 0 9 9 12 0 1 0 3 3 2
Nucleus 71 40 39 29 57 47 50 49 44 45 42 34 58 48 36 47 33 50 37 20 26 51 64
Nuclear Periphery 3 0 2 0 0 1 3 2 1 3 1 2 1 1 1 4 0 0 0 0 0 2 1
Nucleolus 182 76 117 195 194 192 253 208 290 197 196 195 312 288 60 93 74 173 62 118 155 318 306
Peroxisomes 0 0 0 0 2 0 3 1 3 0 1 1 1 5 0 0 0 0 0 0 0 1 0
SpindlePole 6 2 36 119 86 70 192 85 172 87 123 45 95 79 6 14 5 9 4 11 22 63 43
Vac/Vac Membrane 0 3 0 1 3 1 2 2 2 4 4 3 4 3 0 1 1 3 1 4 14 15 10
Unique Cell Count 235 100 155 226 277 285 398 318 406 284 304 241 396 399 95 133 100 252 118 168 234 506 462
Labelled Cell Count 277 137 206 369 385 430 621 471 633 431 475 317 537 535 122 182 133 252 118 168 234 506 462


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.3 8.3 8.6 8.2 8.1 5.2 6.8 5.3 6.0 5.7 5.8 9.7 8.0 9.3 10.3 10.4 10.6 8.7 9.0 9.1
Std Deviation (1e-4) 2.1 1.8 2.4 2.2 2.0 1.9 2.1 1.4 1.6 1.3 1.8 3.1 2.7 3.1 3.6 2.7 2.8 2.1 2.5 2.8
Intensity Change (Log2) -0.06 -0.07 -0.71 -0.33 -0.68 -0.51 -0.59 -0.57 0.18 -0.1 0.12 0.27 0.28 0.3 0.02 0.08 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2.9 3.3 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 -0.2 -0.6 1.4 -0.1 0.1 0 -0.6 1.7 2.1 3.2 0.4 -0.3 -0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.7 0 3.6 0 0 1.3 0 1.2 0 3.6 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 7.4 2.4 6.6 3.9 5.5 5.2 0 0 0 0 0 4.0
Nucleus -3.1 -1.1 -2.2 -3.6 -2.6 -4.3 -2.4 -3.0 -2.8 -2.9 -3.8 2.1 1.9 1.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.7 -1.2 -1.8 -2.7 -2.2 -1.0 -1.4 -2.4 1.3 0.8 -0.8 -2.1 -1.1 -0.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 5.7 1.7 0.3 5.4 0.8 4.2 1.7 3.7 -1.1 0.2 -0.9 -3.5 -2.8 -3.9
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.9184 5.542 5.1092 4.0913 4.2327 5.0659 6.7522 7.5317 6.5906 6.0122 6.7012 6.5147 9.0112 10.1836 8.6571 9.1122 7.4927 8.7889
Actin 0.0399 0.001 0.003 0.0015 0.0156 0.0125 0.0435 0 0.0051 0.0398 0.0022 0.0057 0.0048 0 0.016 0.0099 0.0012 0
Bud 0.0007 0.0002 0.0007 0.0001 0.0004 0.0002 0.0003 0.0002 0.0001 0.0005 0.0002 0.0001 0.0001 0.0001 0.0001 0.0004 0.0001 0
Bud Neck 0.0003 0.0002 0.0003 0.0003 0.0007 0.0003 0.0006 0.0002 0.0007 0.0011 0.0005 0.0012 0.0003 0.0005 0.0011 0.0038 0.0003 0.0003
Bud Periphery 0.0009 0.0003 0.0013 0.0002 0.0005 0.0005 0.0004 0.0002 0.0002 0.0011 0.0005 0.0002 0.0002 0.0001 0.0002 0.0007 0.0001 0.0001
Bud Site 0.0058 0.0024 0.0026 0.0003 0.002 0.0002 0.0012 0.001 0.0008 0.0041 0.0008 0.0007 0.0005 0.0004 0.0004 0.0024 0.0003 0.0001
Cell Periphery 0.0003 0.0002 0.0002 0.0001 0.0004 0.0001 0.0002 0.0001 0.0002 0.0007 0.0004 0.0002 0.0002 0.0004 0.0002 0.0006 0.0001 0.0001
Cytoplasm 0.0009 0.0002 0.0002 0.0001 0.0005 0.0004 0.0048 0 0.0026 0.0001 0.0039 0.0062 0.0047 0 0.0002 0.0005 0.0002 0
Cytoplasmic Foci 0.0165 0.0015 0.0026 0.0139 0.0313 0.0015 0.0122 0 0.0038 0.0002 0.0083 0.0052 0.0038 0.0002 0.001 0.0276 0.0175 0.0001
Eisosomes 0.0004 0 0 0 0.0001 0.0001 0.0003 0 0.0001 0.0024 0.0001 0.0001 0 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0014 0.0001 0.0002 0 0.0003 0.0008 0.003 0 0.0027 0.0004 0.0025 0.0042 0.0021 0 0.0006 0.0001 0 0
Endosome 0.0243 0.0047 0.0084 0.0049 0.0302 0.0077 0.02 0 0.0029 0.0003 0.0141 0.0085 0.013 0.0001 0.0028 0.0024 0.0014 0
Golgi 0.0284 0.0038 0.0014 0.009 0.0657 0.0019 0.0073 0 0.0018 0.0004 0.0022 0.0046 0.0011 0 0.0019 0.0025 0.0011 0
Lipid Particles 0.0218 0.0007 0.0067 0.0125 0.0244 0.0037 0.0158 0.0001 0.0077 0.0004 0.0412 0.0084 0.0029 0.0005 0.0014 0.0046 0.0049 0.0004
Mitochondria 0.0072 0.0052 0.0159 0.0013 0.0308 0.0023 0.005 0 0.0014 0.0043 0.0039 0.0015 0.0009 0.0002 0.0003 0.0088 0.0001 0.0001
None 0.0016 0.0001 0.0001 0 0.0002 0.0006 0.0062 0 0.0023 0.0004 0.0021 0.0011 0.007 0 0.0004 0.0009 0.0002 0
Nuclear Periphery 0.003 0.0001 0.0011 0.0001 0.0002 0.0071 0.0227 0 0.0018 0.0003 0.0167 0.0066 0.0132 0 0.0008 0.0002 0.0007 0
Nucleolus 0.7556 0.8263 0.8685 0.8131 0.6664 0.8579 0.7486 0.8453 0.8386 0.8336 0.7349 0.8263 0.8327 0.8506 0.8793 0.8026 0.7686 0.9113
Nucleus 0.0785 0.1507 0.081 0.1292 0.1055 0.0912 0.084 0.1523 0.1188 0.095 0.1141 0.1066 0.0966 0.1443 0.0906 0.1038 0.1174 0.0825
Peroxisomes 0.0083 0.0003 0.0028 0.0116 0.0217 0.0006 0.0055 0 0.0027 0.0003 0.0026 0.0006 0.0006 0 0.0005 0.0211 0.0086 0
Punctate Nuclear 0.0022 0.001 0.0019 0.0011 0.0009 0.0023 0.0156 0.0004 0.0046 0.0137 0.0461 0.0102 0.0138 0.0009 0.0015 0.0049 0.0769 0.0049
Vacuole 0.0011 0.0006 0.0007 0.0005 0.0011 0.0039 0.0015 0.0001 0.0008 0.001 0.0014 0.0011 0.0008 0.0016 0.0006 0.0018 0.0002 0.0001
Vacuole Periphery 0.0011 0.0002 0.0004 0.0002 0.0012 0.0044 0.0016 0 0.0003 0.0002 0.0013 0.0008 0.0008 0.0001 0.0001 0.0004 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.0318 39.7609 73.7608 46.5686 58.7771 42.7439 42.9081 38.6135 38.6776 54.2969
Translational Efficiency 2.2114 2.8216 1.207 1.6367 1.6923 1.7424 1.5433 1.2873 1.1553 1.2611

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Component of small ribosomal subunit (SSU) processosome; this complex contains U3 snoRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; originally isolated as bud-site selection mutant that displays a random budding pattern
Localization
Cell Percentages nucleolus (78%), nucleus (8%), mixed (13%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Bud21

Bud21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available