Standard name | |
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Human Ortholog | |
Description | Endosomal Rab family GTPase; required for endocytic transport and sorting of vacuolar hydrolases; required for endosomal localization of the CORVET complex; required with YPT52 for MVB biogenesis and sorting; involved in autophagy and ionic stress tolerance; geranylgeranylation required for membrane association; protein abundance increases in response to DNA replication stress; mammalian Rab5 homolog; VPS21 has a paralog, YPT53, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 5 | – | 4 | 0 | 0 | 0 | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | – | 0 | 0 | 1 | 0 | – | 0 | – | – | – | 0 | 0 | 1 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 1 | 1 | 0 | 7 | 5 | 5 | – | 2 | 6 | 6 | 0 | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 135 | 196 | 130 | 131 | 118 | 149 | 138 | 167 | – | 96 | 101 | 105 | 19 | – | 44 | – | – | – | 131 | 193 | 130 | 26 | 48 | 68 |
Endoplasmic Reticulum | 0 | 1 | 0 | 1 | 1 | 4 | 6 | 8 | – | 1 | 4 | 0 | 0 | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 1 | 1 | 1 | 1 | 4 | 4 | – | 2 | 17 | 14 | 1 | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1 | 0 | 1 | 2 | 1 | 2 | 0 | 6 | – | 1 | 1 | 3 | 1 | – | 0 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 1 | 1 | 15 | 8 | – | 9 | 6 | 5 | 0 | – | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 3 |
Unique Cell Count | 136 | 196 | 132 | 135 | 122 | 159 | 160 | 193 | 109 | 128 | 121 | 20 | 44 | 135 | 196 | 135 | 28 | 56 | 78 | |||||
Labelled Cell Count | 137 | 197 | 133 | 137 | 123 | 166 | 168 | 205 | 115 | 137 | 134 | 21 | 44 | 135 | 196 | 135 | 28 | 56 | 78 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 26.1 | 27.8 | 17.8 | 17.3 | 18.1 | 18.0 | 18.6 | 18.3 | – | 16.0 | 17.9 | 17.1 | 18.6 | – | 24.7 | – | – | – | – | 25.0 | 26.0 |
Std Deviation (1e-4) | 3.7 | 3.6 | 3.5 | 4.5 | 4.5 | 4.8 | 4.5 | 5.5 | – | 4.7 | 5.5 | 4.6 | 3.8 | – | 4.3 | – | – | – | – | 4.9 | 9.8 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.02 | 0.02 | 0.06 | 0.04 | – | -0.15 | 0.0 | -0.06 | 0.06 | – | 0.47 | – | – | – | – | 0.49 | 0.54 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – |
Cytoplasm | -0.8 | -0.9 | -2.0 | -3.8 | -3.8 | – | -3.3 | -5.0 | -3.6 | -1.0 | – | 0.8 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 4.0 | 3.6 | 0 | – | 0 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – |
Vacuole | 0 | 0 | 0 | 3.6 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 17.1591 | 21.2319 | 18.5264 | 17.0159 | 16.8619 | 19.0361 | 15.4217 | 20.695 | 20.9229 | 19.8221 | 18.856 | 19.0809 | 18.3737 | 21.6714 | 20.108 | 19.8948 | 19.449 | 20.2961 |
Actin | 0.0096 | 0.0002 | 0.0031 | 0.0027 | 0.0042 | 0.0007 | 0.008 | 0.0002 | 0.0038 | 0.0002 | 0.0034 | 0.0024 | 0.0185 | 0.0003 | 0.0017 | 0.0005 | 0.0122 | 0.0068 |
Bud | 0.0003 | 0.0001 | 0.0005 | 0.0005 | 0.0002 | 0.0002 | 0.0015 | 0 | 0.0006 | 0.0001 | 0.0015 | 0.0003 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0007 |
Bud Neck | 0.0004 | 0.0001 | 0.0004 | 0.0007 | 0.0005 | 0.001 | 0.0011 | 0.0001 | 0.0004 | 0.0009 | 0.0006 | 0.0008 | 0.0009 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0009 |
Bud Periphery | 0.0002 | 0 | 0.0008 | 0.0011 | 0.0002 | 0.0001 | 0.0019 | 0 | 0.0004 | 0 | 0.0005 | 0.0004 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0.0005 |
Bud Site | 0.0007 | 0.0001 | 0.0015 | 0.0031 | 0.0004 | 0.0001 | 0.0021 | 0 | 0.0106 | 0 | 0.0003 | 0.0001 | 0.0008 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0003 |
Cell Periphery | 0.0001 | 0 | 0.0002 | 0.0006 | 0.0001 | 0 | 0.0008 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.8419 | 0.9854 | 0.9455 | 0.8514 | 0.8888 | 0.9239 | 0.7877 | 0.9844 | 0.9574 | 0.9865 | 0.9671 | 0.9428 | 0.8788 | 0.9862 | 0.9519 | 0.9308 | 0.9352 | 0.9223 |
Cytoplasmic Foci | 0.0257 | 0.0005 | 0.005 | 0.0069 | 0.0136 | 0.005 | 0.0159 | 0.0005 | 0.0092 | 0.0009 | 0.0022 | 0.0016 | 0.012 | 0.0006 | 0.0069 | 0.0012 | 0.0048 | 0.0024 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.014 | 0.0005 | 0.002 | 0.002 | 0.0082 | 0.0032 | 0.0274 | 0.0003 | 0.0007 | 0.0006 | 0.0013 | 0.0024 | 0.0042 | 0.0004 | 0.0008 | 0.0005 | 0.0026 | 0.0019 |
Endosome | 0.021 | 0.0002 | 0.0037 | 0.025 | 0.0154 | 0.0038 | 0.0283 | 0.0001 | 0.0014 | 0.0002 | 0.0018 | 0.008 | 0.0128 | 0.0002 | 0.0059 | 0.0021 | 0.0072 | 0.0086 |
Golgi | 0.0042 | 0 | 0.0011 | 0.0044 | 0.0054 | 0.0004 | 0.0041 | 0 | 0.0008 | 0 | 0.0003 | 0.0041 | 0.0038 | 0 | 0.001 | 0.0021 | 0.0042 | 0.0027 |
Lipid Particles | 0.0145 | 0 | 0.0053 | 0.0206 | 0.0129 | 0.002 | 0.0016 | 0 | 0.0008 | 0 | 0 | 0.0018 | 0.0131 | 0 | 0.0046 | 0.0435 | 0.0047 | 0.001 |
Mitochondria | 0.0027 | 0.0001 | 0.0115 | 0.0373 | 0.0035 | 0.0012 | 0.0029 | 0 | 0.0002 | 0.0001 | 0.0023 | 0.0035 | 0.0045 | 0.0001 | 0.0024 | 0.0015 | 0.0058 | 0.0031 |
None | 0.006 | 0.0003 | 0.0008 | 0.0022 | 0.0056 | 0.0008 | 0.0065 | 0.0003 | 0.0009 | 0.0005 | 0.0012 | 0.001 | 0.0018 | 0.0005 | 0.0021 | 0.0002 | 0.0019 | 0.001 |
Nuclear Periphery | 0.0104 | 0.0014 | 0.0022 | 0.0038 | 0.0082 | 0.0132 | 0.0286 | 0.0009 | 0.0012 | 0.0013 | 0.0017 | 0.0084 | 0.0078 | 0.0014 | 0.0017 | 0.0017 | 0.0025 | 0.0088 |
Nucleolus | 0.0002 | 0 | 0.0004 | 0.0009 | 0.0003 | 0.0002 | 0.0006 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 |
Nucleus | 0.0311 | 0.0107 | 0.0124 | 0.0135 | 0.0248 | 0.0372 | 0.0493 | 0.0129 | 0.0081 | 0.0083 | 0.0145 | 0.0194 | 0.0231 | 0.0093 | 0.0125 | 0.009 | 0.015 | 0.0322 |
Peroxisomes | 0.0072 | 0 | 0.0016 | 0.004 | 0.0012 | 0.0008 | 0.0006 | 0 | 0.0009 | 0 | 0.0002 | 0.0003 | 0.0089 | 0 | 0.0038 | 0.0042 | 0.001 | 0.0008 |
Punctate Nuclear | 0.0031 | 0.0001 | 0.0005 | 0.0015 | 0.0016 | 0.0042 | 0.0107 | 0.0002 | 0.0024 | 0.0002 | 0.0004 | 0.0011 | 0.0015 | 0.0002 | 0.0028 | 0.0005 | 0.0003 | 0.0042 |
Vacuole | 0.0048 | 0.0003 | 0.001 | 0.0135 | 0.0036 | 0.0012 | 0.0164 | 0 | 0.0002 | 0.0002 | 0.0004 | 0.0008 | 0.0044 | 0.0001 | 0.0008 | 0.0011 | 0.0009 | 0.0008 |
Vacuole Periphery | 0.0017 | 0.0001 | 0.0005 | 0.004 | 0.0014 | 0.0006 | 0.0039 | 0 | 0.0001 | 0 | 0.0002 | 0.0006 | 0.0012 | 0 | 0.0001 | 0.0004 | 0.0004 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 20.0461 | 35.8399 | 26.5947 | 38.2117 | 30.5107 | 15.6795 | 29.5416 | 37.2187 | 43.8514 | 35.3087 |
Translational Efficiency | 4.3774 | 2.4273 | 2.8332 | 2.3644 | 2.5461 | 4.9739 | 2.8008 | 1.907 | 1.8702 | 2.3723 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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