Standard name
Human Ortholog
Description GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress; RAS1 has a paralog, RAS2, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.71 0.9 0.97 0.85 – 0.83 – 0.66 – 0.55 0.6 – 0.93 0.96 0.96 0.94 0.94 0.89 0.83 0.87 0.92 0.88 0.86 0.83
Endoplasmic Reticulum 0 0 0 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 – 0.26 – 0.41 – 0.5 0.57 – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.15 0 0.2 – 0 – 0.12 – 0.15 0.06 – 0.1 0 0 0 0.09 0.13 0 0 0 0.06 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0 1 3 1 0 2 0
Bud 0 0 0 1 – 1 – 3 – 7 9 – 1 0 0 0 0 0 0 4 0 0 4 4
Bud Neck 0 0 0 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0 0 2 0 0 2 1
Bud Site 0 0 0 0 – 0 – 0 – 0 1 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 – 3 – 3 – 4 0 – 1 1 1 1 4 0 0 0 0 0 0 0
Cytoplasm 34 520 36 154 – 333 – 463 – 245 244 – 436 378 490 170 250 196 48 525 231 268 423 388
Endoplasmic Reticulum 1 1 0 1 – 2 – 2 – 3 0 – 9 6 10 4 7 4 0 2 2 2 3 6
Endosome 0 2 0 3 – 2 – 1 – 1 1 – 0 0 0 1 0 2 0 5 1 0 6 5
Golgi 0 3 0 0 – 0 – 0 – 0 0 – 1 0 0 0 0 1 0 4 1 1 4 3
Mitochondria 3 2 1 5 – 103 – 288 – 225 234 – 0 0 0 3 1 2 0 3 0 1 2 8
Nucleus 2 6 0 0 – 12 – 15 – 17 11 – 4 5 12 8 6 8 2 24 3 9 9 10
Nuclear Periphery 0 0 0 0 – 0 – 2 – 1 3 – 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 – 0 – 0 – 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0 0 1 0 0 2 2
Vac/Vac Membrane 9 86 0 37 – 7 – 88 – 66 23 – 46 13 11 8 23 29 2 14 2 17 25 33
Unique Cell Count 48 581 37 182 401 704 449 407 469 395 511 181 265 221 58 602 251 306 492 471
Labelled Cell Count 49 620 37 202 463 865 569 527 498 403 524 195 291 242 58 602 251 306 492 471


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 8.2 6.4 6.2 – 4.7 – 4.4 – 4.1 4.2 – 8.6 8.2 8.2 10.3 10.8 11.2 9.3 9.2 8.9
Std Deviation (1e-4) 1.5 1.2 1.1 1.0 – 0.9 – 0.9 – 0.9 0.9 – 1.0 0.9 1.0 1.8 1.7 1.8 1.3 1.3 1.2
Intensity Change (Log2) – – – -0.05 – -0.43 – -0.55 – -0.65 -0.61 – 0.42 0.35 0.36 0.69 0.76 0.81 0.55 0.52 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0
Bud 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0
Bud Neck 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0
Bud Site 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0
Cell Periphery 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0
Cytoplasm -2.1 – -2.3 – -4.0 – -5.1 -4.5 – -1.0 -0.5 -0.4 -0.8 -0.8 -1.6
Endoplasmic Reticulum 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0
Endosome 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0
Golgi 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0
Mitochondria 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0
Nucleus 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0
Nucleolus 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0
Peroxisomes 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0
SpindlePole 0 – 0 – 0 – 0 0 – 0 0 0 0 0 0
Vacuole 3.0 – 0 – 0 – 2.5 0 – 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4615 5.384 4.62 4.3527 4.0586 4.8432 7.6212 8.7625 8.2128 7.5561 7.7257 8.1601 5.5557 6.9591 6.0711 5.5324 5.9101 6.154
Actin 0.0038 0.0003 0.0002 0.0004 0.0001 0.0003 0.0015 0.0009 0.0004 0.0041 0.0004 0.0006 0.0017 0.0002 0.0002 0.0003 0.0002 0.0001
Bud 0.0005 0.0003 0.0001 0.0002 0.0001 0.001 0.0001 0.0018 0.0018 0.0003 0.0015 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0006 0.0006 0.0005 0.001 0.0008 0.0017 0.0006 0.0005 0.0007 0.0022 0.0018 0.002 0.0006 0.0005 0.0007 0.001 0.0008 0.0017
Bud Periphery 0.0002 0.0001 0 0 0 0.0002 0.0001 0.0005 0.0003 0.0001 0.0002 0 0.0001 0 0 0 0 0
Bud Site 0.0005 0.0013 0.0003 0.0003 0.0001 0.0001 0.0004 0.0019 0.0004 0.0006 0.0003 0.0001 0.0005 0.0004 0.0002 0.0001 0.0001 0.0001
Cell Periphery 0.0001 0 0 0 0 0 0.0001 0.0001 0 0.0001 0 0 0 0 0 0 0 0
Cytoplasm 0.7245 0.7401 0.758 0.7454 0.7089 0.7229 0.8418 0.8314 0.7964 0.7718 0.8084 0.7706 0.7759 0.7814 0.7329 0.7345 0.8047 0.7289
Cytoplasmic Foci 0.0105 0.003 0.0044 0.0053 0.0104 0.0067 0.005 0.0043 0.0035 0.0051 0.0053 0.0058 0.0039 0.0026 0.0022 0.0027 0.0035 0.0028
Eisosomes 0.0002 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0073 0.0019 0.0027 0.0046 0.0024 0.0029 0.0046 0.0023 0.0033 0.0074 0.0022 0.0032 0.0034 0.0017 0.0024 0.0025 0.0022 0.002
Endosome 0.0156 0.0018 0.0048 0.0067 0.0142 0.0072 0.0033 0.0028 0.0024 0.005 0.0016 0.0039 0.0026 0.0012 0.0018 0.0017 0.0022 0.0025
Golgi 0.0012 0.0001 0.0001 0.0001 0.0002 0.0002 0.0009 0.0001 0.0001 0.0008 0.0001 0.0001 0.0002 0 0.0001 0 0 0
Lipid Particles 0.0023 0 0.0001 0 0 0.0007 0.0003 0 0 0.0001 0 0.0001 0.0002 0 0 0 0 0
Mitochondria 0.0008 0.0003 0.0003 0.0005 0.0005 0.0004 0.0003 0.0003 0.0003 0.0005 0.0002 0.0003 0.0005 0.0002 0.0003 0.0003 0.0003 0.0002
None 0.0801 0.0173 0.0275 0.0169 0.0096 0.0167 0.018 0.024 0.0179 0.0087 0.0048 0.0253 0.0191 0.007 0.0131 0.01 0.0152 0.0086
Nuclear Periphery 0.0152 0.0084 0.0089 0.0136 0.0066 0.0088 0.0069 0.0051 0.0058 0.0087 0.0057 0.0073 0.0072 0.0051 0.0067 0.0079 0.0063 0.0062
Nucleolus 0.0009 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0003 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001
Nucleus 0.1217 0.2183 0.1847 0.1962 0.2414 0.2183 0.1112 0.1189 0.1619 0.1774 0.1518 0.1703 0.1773 0.1955 0.2342 0.2325 0.157 0.2405
Peroxisomes 0.0012 0.0001 0.0001 0.0002 0.0001 0.0035 0.0004 0.0005 0.0001 0.0005 0.0001 0.0002 0.0012 0.0001 0.0001 0.0001 0.0001 0.0001
Punctate Nuclear 0.009 0.0049 0.0051 0.0062 0.0026 0.006 0.0034 0.0034 0.0034 0.0055 0.0147 0.0087 0.0042 0.0031 0.004 0.0048 0.0067 0.005
Vacuole 0.0031 0.0009 0.0014 0.0017 0.0015 0.002 0.0009 0.0009 0.0009 0.001 0.0006 0.0009 0.001 0.0006 0.0007 0.001 0.0005 0.0008
Vacuole Periphery 0.0007 0.0002 0.0003 0.0004 0.0004 0.0004 0.0001 0.0002 0.0002 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.4411 28.6367 48.0086 37.3074 43.8413 24.9018 48.7938 38.361 33.0587 35.7652
Translational Efficiency 1.5444 2.393 1.1468 1.5099 0.9948 1.6283 1.1532 1.2858 1.0594 1.319

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress; RAS1 has a paralog, RAS2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ras1

Ras1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ras1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available