Standard name
Human Ortholog
Description Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.14 0.07 0 0 0 0 0 0 0 0 – – – 0.09 0.08 0.13 0.05 0.05 0.08 0 0.07 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0.06 0
Golgi 0 0.11 0.47 0.23 0.42 0 0.05 0.1 0 0.06 0 0 – – – 0.66 0.56 0.59 0.11 0.17 0.11 0.29 0.22 0.34
Mitochondria 0.93 0.87 0.59 0.74 0.74 0.85 0.79 0.75 0.87 0.84 0.9 0.87 – – – 0.1 0.17 0.08 0.76 0.71 0.72 0.59 0.45 0.38
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.05 0.09 0.05 0.08 0.09 0.08 0.07 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 0.07 0 0.08 0.08 0.14 0.12 0.1 0.09 0.09 0.12 0.1 – – – 0.16 0.16 0.14 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0.05 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.09 0.14 0.05 0.09 0.06 0.06 0.07 0.07 0.06 0.05 0 – – – 0.09 0.11 0.09 0 0 0 0 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 6 15 10 9 3 1 4 1 1 0 2 – – – 27 17 28 13 18 11 0 0 0
Bud 0 3 0 1 0 2 4 5 8 3 4 6 – – – 1 0 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 0 1 – – – 0 0 1 – – – – – –
Cell Periphery 1 5 6 2 2 1 1 7 4 1 1 3 – – – 8 7 6 0 0 0 0 0 0
Cytoplasm 6 4 0 11 6 6 11 15 6 5 3 2 – – – 3 3 3 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 3 3 2 2 1 0 – – – 2 6 4 1 1 0 0 0 0
Endosome 0 0 0 1 1 4 6 4 3 3 2 3 – – – 2 0 2 1 2 1 0 0 0
Golgi 10 37 52 34 87 10 15 36 4 12 2 8 – – – 192 117 127 31 63 16 4 2 5
Mitochondria 259 279 65 108 152 199 244 266 219 169 213 207 – – – 28 35 18 223 260 104 10 5 5
Nucleus 1 0 1 0 0 0 0 1 0 2 0 1 – – – 2 2 2 1 0 0 0 0 0
Nuclear Periphery 5 1 0 0 3 12 27 19 20 17 18 16 – – – 2 0 0 1 1 0 0 0 0
Nucleolus 28 22 3 12 17 33 36 37 23 18 28 25 – – – 46 34 29 2 3 0 0 0 0
Peroxisomes 4 6 3 3 5 1 8 8 4 2 3 5 – – – 2 5 11 10 4 3 0 0 0
SpindlePole 0 0 0 0 0 0 2 7 3 2 2 6 – – – 1 1 5 0 1 1 0 0 0
Vac/Vac Membrane 7 29 15 7 18 13 18 26 17 11 12 6 – – – 26 24 20 3 6 1 0 0 1
Unique Cell Count 278 322 110 145 205 235 310 357 253 200 237 239 290 209 214 293 366 146 17 13 15
Labelled Cell Count 326 392 160 189 300 284 376 438 314 249 289 291 342 251 257 293 366 146 17 13 15


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 31.0 39.0 33.7 31.2 27.9 20.5 18.7 17.4 13.5 13.3 11.3 11.4 – – – 44.5 45.3 44.9 – – –
Std Deviation (1e-4) 10.1 11.2 8.7 9.6 6.8 5.9 5.4 4.7 3.9 3.7 3.7 3.5 – – – 17.5 17.4 17.9 – – –
Intensity Change (Log2) – – – -0.11 -0.27 -0.72 -0.85 -0.95 -1.32 -1.34 -1.58 -1.56 – – – 0.4 0.43 0.41 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.8 -2.9 -4.8 0 0 0 -5.0 0 -5.2 – – – -1.3 -1.6 -0.1
Bud 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Golgi -4.0 -0.8 -9.7 -10.4 -8.7 -11.1 -8.6 -11.1 -10.1 – – – 3.5 1.5 2.1
Mitochondria 2.6 2.8 5.2 4.0 3.1 5.8 5.0 6.7 5.8 – – – -10.5 -7.7 -9.9
Nucleus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 3.2 0 3.0 3.1 3.0 2.8 – – – 0 0 0
Nucleolus 1.9 1.9 3.2 2.8 2.5 2.2 2.1 2.8 2.5 – – – 3.6 3.6 3.1
Peroxisomes 0 0 0 0 0 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Vacuole -2.5 -1.3 -2.6 -2.6 -2.1 -2.1 -2.5 -2.8 -4.1 – – – -1.4 -0.6 -1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 29.9986 41.3405 39.7129 28.4565 33.8165 38.7544 37.447 39.9722 36.9262 35.8972 28.1497 38.0954 40.5281 47.3716 44.0576 39.5422 40.2629 45.9071
Actin 0.0148 0.0052 0.0013 0.0339 0.0001 0.0005 0.008 0.0064 0.0067 0.0178 0.0013 0.0106 0.0106 0.006 0.0024 0.002 0 0.0001
Bud 0.0014 0.0004 0 0.0004 0 0 0.0001 0.0001 0.0005 0.001 0.0001 0.0001 0.0002 0 0.0001 0 0 0
Bud Neck 0.0004 0.0002 0 0.0002 0 0 0.0005 0.0001 0.0006 0.0003 0 0.0008 0.0005 0 0.0002 0 0 0
Bud Periphery 0.0032 0.0004 0 0.0009 0 0 0.0001 0.0002 0.0005 0.0028 0.0001 0.0002 0.0003 0.0001 0.0002 0 0 0
Bud Site 0.0072 0.0004 0 0.0013 0 0 0.0028 0.0002 0.0071 0.0006 0.0001 0.0002 0.0008 0.0001 0.0029 0.0001 0 0
Cell Periphery 0.0005 0.0011 0 0.0002 0 0.0001 0.0002 0.0002 0.0008 0.0003 0.0002 0.0001 0.0004 0.0002 0.0003 0.0001 0 0.0001
Cytoplasm 0.0012 0.0004 0 0.0001 0 0 0.002 0 0.0005 0.0011 0.0002 0.0001 0.0003 0 0.0002 0.0001 0 0
Cytoplasmic Foci 0.0189 0.0008 0.0001 0.0066 0 0.0004 0.0145 0.0002 0.0053 0.0013 0.0104 0.0002 0.0089 0.0001 0.0022 0.0168 0 0.0003
Eisosomes 0.0004 0.0007 0.0004 0.0029 0.0001 0.0003 0.0015 0.0009 0.0012 0.0003 0.0003 0.001 0.0006 0.0009 0.0004 0.0004 0.0002 0.0002
Endoplasmic Reticulum 0.0006 0.0001 0 0.0001 0 0 0.0007 0 0.0002 0.0016 0.0007 0.0006 0.0002 0 0.0001 0 0 0
Endosome 0.0073 0.002 0.0001 0.0269 0 0.0009 0.0043 0.0007 0.0028 0.0108 0.0412 0.0019 0.0011 0.0003 0.0014 0.0001 0 0
Golgi 0.0025 0.0035 0.0003 0.0082 0.0005 0.0028 0.0019 0.0122 0.0059 0.0055 0.0056 0.0015 0.0014 0.0061 0.0013 0.0003 0 0.0001
Lipid Particles 0.0195 0.0107 0.0024 0.0283 0.0007 0.0272 0.0232 0.008 0.0115 0.0049 0.0169 0.001 0.0121 0.0064 0.0142 0.0075 0.0001 0.001
Mitochondria 0.8767 0.9401 0.9907 0.8305 0.997 0.929 0.9242 0.9613 0.9381 0.8878 0.8956 0.9698 0.9367 0.9658 0.9522 0.9637 0.9994 0.984
None 0.0009 0 0 0.0003 0 0 0.0025 0 0.0003 0.0005 0.0004 0.0005 0.0012 0 0.0015 0.0012 0 0
Nuclear Periphery 0.0009 0.0003 0 0.0003 0 0.0003 0.0003 0 0.0003 0.0114 0.0127 0.0045 0.0015 0 0.0005 0.0001 0 0
Nucleolus 0.0068 0.0004 0.0001 0.0006 0 0.0003 0.0014 0.0003 0.0051 0.0003 0.0005 0.0005 0.0092 0.0001 0.0015 0.0012 0 0.0069
Nucleus 0.001 0.0001 0 0.0002 0 0.0001 0.0002 0.0001 0.0004 0.0017 0.0006 0.0013 0.0015 0 0.0003 0.0001 0 0.0003
Peroxisomes 0.0163 0.0023 0.0021 0.0417 0.0004 0.0027 0.0058 0.0022 0.0043 0.001 0.0057 0.0009 0.0043 0.0012 0.0045 0.0038 0.0001 0.0003
Punctate Nuclear 0.004 0 0 0.001 0 0 0.0016 0 0.001 0.0062 0.0013 0.0009 0.0004 0 0.0012 0.0005 0 0
Vacuole 0.0084 0.0067 0.0002 0.0106 0.0001 0.0012 0.0005 0.0008 0.001 0.0311 0.0015 0.0003 0.0019 0.0009 0.0014 0.0002 0 0.0003
Vacuole Periphery 0.0071 0.0244 0.0023 0.0046 0.0009 0.0342 0.0037 0.006 0.0061 0.0116 0.0046 0.0031 0.0058 0.0115 0.0111 0.0017 0.0001 0.0064

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 85.1111 101.0832 91.1388 74.2801 81.5804 66.836 79.1265 68.7961 53.1406 68.9082
Translational Efficiency 0.9029 0.8311 0.7713 0.9048 0.8288 0.8901 0.8097 0.8572 0.8536 0.7193

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Leu9

Leu9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Leu9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available