Standard name
Human Ortholog
Description Component of nuclear aminoacylation-dependent tRNA export pathway; cytoplasmic; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a; required for activation of RAN GTPase Gsp1p and dissociation of receptor-tRNA-Gsp1p export complex; recruits Rna1p from cytoplasm to NPC, facilitates Rna1p activation of Gsp1p GTPase activity by enabling Rna1p to gain access to Gsp1p-GTP bound to export receptor tRNA complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.09 0.06 0.13 0 0 0 0 0.05 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.79 0.79 0.77 0.91 0.87 0.77 0.75 0.75 0.66 0.58 0.54 0.62 0.8 0.89 0.85 0.7 0.78 – 0.56 0.64 0.46 0.35 0.43 0.49
Endoplasmic Reticulum 0.05 0.12 0.09 0 0 0.05 0 0.06 0 0 0 0 0 0 0.06 0.05 0.05 – 0.05 0 0 0.16 0.2 0.18
Endosome 0.06 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0.05 0 – 0.12 0.09 0.08 0.07 0 0
Golgi 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.11 0 0.06 0.1 0.05 0.05
Mitochondria 0 0 0 0.09 0 0.18 0.22 0.13 0.4 0.48 0.54 0.39 0.09 0 0 0 0 – 0 0 0.21 0.15 0.12 0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.14 0.11 – 0 0.07 0.09 0.1 0.08 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 14 14 30 3 5 4 11 13 4 3 2 2 4 4 5 11 6 – 4 6 4 5 9 9
Bud 3 1 4 2 0 5 3 6 7 14 20 9 3 0 1 0 1 – 2 4 1 3 6 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 3 3 1 3 4
Bud Site 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 – – – – – – –
Cell Periphery 1 5 3 3 9 6 4 3 10 10 8 7 2 4 3 9 1 – 0 0 0 0 0 1
Cytoplasm 129 173 178 182 216 186 275 198 206 188 163 213 207 303 307 150 114 – 109 175 143 92 169 209
Endoplasmic Reticulum 9 26 21 3 9 11 14 16 4 4 5 5 9 14 22 11 8 – 10 12 8 41 80 76
Endosome 10 3 7 3 9 6 11 3 5 4 7 4 22 12 18 11 6 – 23 25 24 19 12 15
Golgi 9 9 2 0 1 0 0 0 0 0 0 0 2 3 4 7 2 – 21 12 19 25 21 22
Mitochondria 7 3 2 18 10 44 81 35 124 155 161 134 23 12 14 7 0 – 7 10 66 39 46 41
Nucleus 0 1 1 1 2 1 4 6 0 2 1 2 0 0 2 1 4 – 0 1 0 0 1 0
Nuclear Periphery 0 0 0 0 0 1 0 0 0 1 0 1 0 1 0 0 0 – 2 0 1 0 1 1
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 3 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 2 0 0 1 1 2 1 3 1 1 0 0 0 1 1 – 5 1 4 2 1 1
Vac/Vac Membrane 1 4 3 6 2 5 6 11 13 13 12 17 9 3 10 30 16 – 7 18 29 26 33 27
Unique Cell Count 164 219 230 201 248 242 369 263 311 324 300 344 259 340 363 214 146 198 276 312 265 394 427
Labelled Cell Count 183 239 253 221 263 270 411 293 374 397 381 396 281 357 387 241 159 198 276 312 265 394 427


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 9.9 7.6 7.0 7.4 6.1 6.1 6.2 5.5 5.4 5.0 5.4 7.7 8.0 8.3 12.0 11.7 – 7.5 7.6 7.9
Std Deviation (1e-4) 1.0 1.4 0.9 1.0 1.2 1.2 1.1 1.2 1.1 1.4 1.0 0.9 1.1 1.1 1.2 2.3 2.2 – 1.2 1.4 1.4
Intensity Change (Log2) – – – -0.12 -0.04 -0.32 -0.32 -0.29 -0.47 -0.51 -0.61 -0.5 0.01 0.06 0.12 0.65 0.62 – -0.02 -0.0 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -4.5 -4.6 -4.8 -4.7 -3.2 -5.6 -5.9 -5.9 -6.4 -5.0 -5.9 -5.9 -2.9 -2.9 –
Bud 0 0 0 0 0 0 1.7 2.7 0.7 0 0 0 0 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 1.6 0 0 0 1.4 1.4 0 0 0 0 0 1.9 0 –
Cytoplasm 3.7 2.8 -0.1 -0.8 -0.5 -2.8 -4.7 -5.5 -3.9 0.7 3.8 2.2 -1.7 0.2 –
Endoplasmic Reticulum -3.4 -2.5 -2.0 -2.7 -1.3 -4.3 -4.4 -3.9 -4.3 -2.6 -2.4 -1.4 -1.6 -1.3 –
Endosome 0 0.4 -0.4 0 0 -1.1 0 -0.5 0 2.5 0.3 1.1 1.1 0.6 –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Mitochondria 4.0 2.2 6.3 7.3 5.2 10.6 12.1 13.1 10.5 4.0 2.0 2.2 0 0 –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Vacuole 0 0 0 0 1.9 2.0 1.9 1.9 2.3 1.5 0 1.2 5.1 4.2 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7147 3.6176 3.1976 2.7518 2.1909 3.278 3.7013 5.2714 4.623 4.3105 4.439 4.6537 2.8203 4.5036 3.5896 3.5731 3.8389 3.9347
Actin 0.0802 0.0029 0.0134 0.0131 0.0066 0.006 0.0439 0.0024 0.0273 0.0232 0.0213 0.0029 0.0194 0.0023 0.0052 0.0018 0.0188 0.0028
Bud 0.0009 0.0008 0.0024 0.0006 0.0004 0.0004 0.0031 0.0023 0.0018 0.0012 0.0024 0.0007 0.0006 0.0006 0.0005 0.0005 0.0005 0.0031
Bud Neck 0.0035 0.0004 0.0008 0.0006 0.0004 0.0035 0.0034 0.0005 0.0015 0.0011 0.0012 0.0015 0.0064 0.0003 0.0007 0.001 0.0006 0.0011
Bud Periphery 0.0009 0.0003 0.0012 0.0003 0.0004 0.0002 0.0036 0.0007 0.0013 0.001 0.0017 0.0003 0.0005 0.0002 0.0003 0.0002 0.0004 0.0025
Bud Site 0.0039 0.0052 0.0079 0.0008 0.0005 0.0005 0.0057 0.0139 0.0164 0.002 0.0214 0.0004 0.0057 0.0028 0.001 0.0007 0.0011 0.0003
Cell Periphery 0.0006 0.0003 0.0002 0.0001 0.0001 0.0001 0.0004 0.0002 0.0003 0.0001 0.0001 0.0001 0.0003 0.0002 0.0001 0 0 0.0002
Cytoplasm 0.3195 0.6527 0.4998 0.3172 0.3752 0.5058 0.4213 0.6163 0.4117 0.3319 0.3861 0.4759 0.4115 0.6752 0.4393 0.3298 0.3133 0.5446
Cytoplasmic Foci 0.1257 0.1072 0.1073 0.1203 0.0919 0.1015 0.1206 0.1119 0.1751 0.1484 0.1631 0.1245 0.1437 0.1166 0.1494 0.1443 0.1388 0.1291
Eisosomes 0.0008 0 0.0002 0 0.0001 0 0.0007 0 0.0001 0.0001 0.0001 0 0.0008 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0247 0.0122 0.0104 0.0129 0.0091 0.0151 0.0072 0.0077 0.005 0.0204 0.0025 0.0159 0.0117 0.0073 0.0067 0.0084 0.0066 0.0064
Endosome 0.2578 0.1645 0.2734 0.4344 0.3907 0.2775 0.256 0.1906 0.261 0.3294 0.2994 0.287 0.2402 0.1494 0.2873 0.4279 0.3802 0.2455
Golgi 0.052 0.0241 0.0332 0.0292 0.0559 0.0475 0.067 0.0381 0.0586 0.0641 0.0417 0.066 0.0512 0.0299 0.0351 0.0415 0.0466 0.0361
Lipid Particles 0.0258 0.0016 0.0038 0.0029 0.0191 0.0007 0.0102 0.0008 0.0032 0.0123 0.0104 0.0004 0.0177 0.0003 0.0051 0.0052 0.0017 0.0043
Mitochondria 0.0171 0.0039 0.0049 0.0083 0.0146 0.0066 0.0165 0.0041 0.0161 0.0106 0.0078 0.0128 0.0139 0.0024 0.0232 0.0026 0.0053 0.0097
None 0.0137 0.0086 0.0166 0.0029 0.0018 0.0194 0.0222 0.0019 0.0016 0.0017 0.0012 0.0014 0.0326 0.0031 0.0084 0.0028 0.0033 0.0014
Nuclear Periphery 0.0074 0.0015 0.0047 0.005 0.0077 0.0014 0.001 0.0006 0.0009 0.0027 0.0013 0.0008 0.0076 0.0007 0.0032 0.0016 0.0413 0.0015
Nucleolus 0.0005 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0003 0 0.0005 0 0.0002 0.0001 0.0002 0.0001
Nucleus 0.0028 0.0008 0.0016 0.0042 0.0012 0.0012 0.0006 0.0007 0.0006 0.0012 0.0009 0.0006 0.0018 0.0005 0.0013 0.0006 0.0051 0.0005
Peroxisomes 0.0223 0.001 0.0037 0.0284 0.0025 0.001 0.0069 0.0014 0.0085 0.0293 0.0195 0.0018 0.0178 0.0023 0.02 0.0051 0.0229 0.0043
Punctate Nuclear 0.0256 0.0007 0.0011 0.0039 0.0015 0.0005 0.0007 0.0003 0.0006 0.0008 0.002 0.0003 0.0076 0.0002 0.0038 0.0004 0.0014 0.0003
Vacuole 0.011 0.0079 0.0094 0.0094 0.0119 0.0075 0.0066 0.0041 0.0057 0.0124 0.0115 0.0038 0.0059 0.0042 0.0065 0.0193 0.007 0.0035
Vacuole Periphery 0.0034 0.0032 0.0037 0.0054 0.0083 0.0034 0.0023 0.0015 0.0025 0.0058 0.004 0.0029 0.0029 0.0015 0.0028 0.006 0.005 0.0026

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.754 18.1224 15.6889 19.4273 26.91 32.3216 19.8505 18.698 19.4435 24.9944
Translational Efficiency 0.8738 1.0008 0.9 0.8504 0.696 0.6811 1.0 0.8728 0.8037 0.6611

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of nuclear aminoacylation-dependent tRNA export pathway; cytoplasmic; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a; required for activation of RAN GTPase Gsp1p and dissociation of receptor-tRNA-Gsp1p export complex; recruits Rna1p from cytoplasm to NPC, facilitates Rna1p activation of Gsp1p GTPase activity by enabling Rna1p to gain access to Gsp1p-GTP bound to export receptor tRNA complex
Localization
Cell Percentages cytoplasm (47%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-3

Cex1

Cex1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cex1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available