Standard name | |
---|---|
Human Ortholog | |
Description | RNA polymerase III largest subunit C160; part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 2 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 2 | 1 | 7 | 5 | 12 | 8 | 10 | 1 | 13 | 0 | 0 | 3 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 13 | 6 | 8 | 10 | 7 | 8 | 6 | 4 | 9 | 8 | 6 | 9 | 25 | 18 | 14 | 12 | 22 | 0 | 1 | 0 | 0 | 2 | 0 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 1 | 1 | 1 | 1 | 2 | 0 | 0 | 2 | 0 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 |
Mitochondria | 0 | 5 | 9 | 11 | 14 | 37 | 60 | 85 | 94 | 93 | 43 | 90 | 0 | 0 | 6 | 8 | 4 | 4 | 0 | 1 | 1 | 0 | 1 | 2 |
Nucleus | 404 | 219 | 104 | 155 | 245 | 207 | 298 | 351 | 205 | 206 | 122 | 181 | 105 | 151 | 141 | 190 | 137 | 190 | 346 | 224 | 105 | 84 | 125 | 81 |
Nuclear Periphery | 0 | 1 | 0 | 2 | 2 | 5 | 7 | 6 | 4 | 11 | 7 | 9 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 136 | 65 | 33 | 16 | 22 | 36 | 73 | 133 | 73 | 79 | 47 | 76 | 31 | 14 | 47 | 44 | 43 | 56 | 118 | 35 | 17 | 14 | 28 | 38 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 6 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 3 | 3 | 0 | 2 | 6 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 5 | 7 | 8 | 11 | 3 | 11 | 4 | 2 | 23 | 38 |
Unique Cell Count | 460 | 262 | 125 | 169 | 273 | 239 | 368 | 446 | 274 | 300 | 165 | 252 | 122 | 181 | 190 | 236 | 191 | 262 | 477 | 284 | 135 | 106 | 193 | 166 |
Labelled Cell Count | 548 | 309 | 153 | 196 | 300 | 300 | 453 | 599 | 390 | 410 | 229 | 379 | 145 | 194 | 236 | 268 | 209 | 289 | 477 | 284 | 135 | 106 | 193 | 166 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 18.3 | 15.2 | 9.1 | 9.5 | 8.3 | 6.9 | 6.2 | 5.8 | 5.1 | 5.3 | 5.3 | 4.7 | 15.8 | 15.5 | 12.4 | 16.8 | 18.6 | 20.1 | 15.0 | 15.9 | 17.9 |
Std Deviation (1e-4) | 4.1 | 6.3 | 2.2 | 2.4 | 1.9 | 1.7 | 1.7 | 1.5 | 1.3 | 1.5 | 1.8 | 1.5 | 5.7 | 5.5 | 4.8 | 6.6 | 6.1 | 7.9 | 4.0 | 7.1 | 7.1 |
Intensity Change (Log2) | – | – | – | 0.07 | -0.13 | -0.39 | -0.55 | -0.66 | -0.85 | -0.79 | -0.78 | -0.96 | 0.8 | 0.77 | 0.45 | 0.88 | 1.03 | 1.15 | 0.72 | 0.81 | 0.98 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | -0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 2.6 | 1.5 | 0.4 | 0.6 | 1.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.2 | -0.8 | 2.3 | 2.5 | 3.2 | 5.7 | 5.2 | 4.1 | 0 | 0 | 0 | -1.6 | -1.6 | -2.2 | 0 |
Nucleus | 2.2 | 1.8 | 0.9 | -0.6 | -1.1 | -1.9 | -3.1 | -1.9 | -2.4 | 0.6 | 0.1 | -1.9 | -0.6 | -2.3 | -2.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -3.9 | -4.9 | -2.6 | -1.5 | 0.7 | 0.1 | 0 | 0.4 | 0 | -0.2 | -4.5 | -0.3 | -1.7 | -0.8 | -1.1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.4052 | 1.3333 | 1.6171 | 1.0563 | 0.9344 | 1.5113 | 1.9028 | 1.9105 | 2.0298 | 1.6419 | 1.5594 | 1.66 | 0.0525 | 0.4555 | 0.1213 | 0.1147 | 0.9645 | 0.0828 |
Actin | 0.0326 | 0.0001 | 0.0083 | 0.0001 | 0.0001 | 0.0015 | 0.0264 | 0.0001 | 0.0128 | 0.024 | 0.0253 | 0.0008 | 0.0978 | 0.0046 | 0.009 | 0 | 0.0163 | 0.0004 |
Bud | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0008 | 0.0004 | 0.0016 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0006 | 0.0001 |
Bud Neck | 0.013 | 0.0008 | 0.0009 | 0.0002 | 0.0006 | 0.004 | 0.0147 | 0.0003 | 0.0012 | 0.0005 | 0.0006 | 0.0016 | 0.0003 | 0.0007 | 0.0006 | 0.0001 | 0.0009 | 0.0033 |
Bud Periphery | 0.0021 | 0.0002 | 0.0001 | 0 | 0.0006 | 0.0002 | 0.0019 | 0.0002 | 0.0005 | 0.0003 | 0.0005 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0009 | 0.0002 |
Bud Site | 0.0127 | 0.001 | 0.0004 | 0 | 0.0002 | 0.0004 | 0.0044 | 0.0009 | 0.007 | 0.001 | 0.0002 | 0.0002 | 0.0008 | 0.0006 | 0.0039 | 0 | 0.0019 | 0.0007 |
Cell Periphery | 0.0041 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0.0009 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0054 | 0.0124 | 0.0032 | 0.001 | 0.0011 | 0.0027 | 0.0047 | 0.0121 | 0.0042 | 0.0003 | 0.0373 | 0.0009 | 0.0064 | 0.0241 | 0.0044 | 0.0002 | 0.0099 | 0.0043 |
Cytoplasmic Foci | 0.0047 | 0.0008 | 0.0083 | 0 | 0.0005 | 0.003 | 0.0143 | 0.0045 | 0.0148 | 0.0076 | 0.0011 | 0.0019 | 0.0094 | 0.0156 | 0.0031 | 0 | 0.0252 | 0.0035 |
Eisosomes | 0.001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0044 | 0.0006 | 0.0004 | 0.0001 | 0.0011 | 0.0003 | 0.0012 | 0.0004 | 0.0005 | 0.0001 | 0.0017 | 0.0003 | 0.0003 | 0.0005 | 0.0003 | 0 | 0.0012 | 0.0002 |
Endosome | 0.0075 | 0.0023 | 0.0047 | 0.0003 | 0.0234 | 0.0028 | 0.0396 | 0.0041 | 0.0107 | 0.0014 | 0.0048 | 0.0048 | 0.0043 | 0.0108 | 0.0011 | 0 | 0.0183 | 0.0008 |
Golgi | 0.0028 | 0.0004 | 0.0036 | 0 | 0.0018 | 0.0017 | 0.0178 | 0.0005 | 0.012 | 0.0025 | 0.008 | 0.0026 | 0.0102 | 0.0169 | 0.0022 | 0 | 0.0231 | 0.0002 |
Lipid Particles | 0.0084 | 0.0007 | 0.0085 | 0.0001 | 0.0019 | 0.0076 | 0.0275 | 0.0001 | 0.0033 | 0.0029 | 0.0004 | 0.0045 | 0.0054 | 0.0356 | 0.0016 | 0 | 0.0088 | 0.0015 |
Mitochondria | 0.0086 | 0.0005 | 0.0008 | 0.0003 | 0.0093 | 0.001 | 0.0175 | 0.0085 | 0.0047 | 0.0039 | 0.0101 | 0.0029 | 0.002 | 0.0025 | 0.0023 | 0.0001 | 0.057 | 0.0003 |
None | 0.0208 | 0.0171 | 0.013 | 0.001 | 0.0006 | 0.0024 | 0.0222 | 0.0097 | 0.0098 | 0.0003 | 0.0075 | 0.0007 | 0.003 | 0.0033 | 0.015 | 0.0001 | 0.1288 | 0.0188 |
Nuclear Periphery | 0.0092 | 0.007 | 0.0082 | 0.0262 | 0.0132 | 0.0036 | 0.0059 | 0.0072 | 0.0028 | 0.0006 | 0.0041 | 0.0028 | 0.0045 | 0.0048 | 0.0309 | 0.001 | 0.0129 | 0.0037 |
Nucleolus | 0.0305 | 0.0348 | 0.0296 | 0.0148 | 0.0277 | 0.0446 | 0.0177 | 0.0183 | 0.0227 | 0.0133 | 0.0381 | 0.0258 | 0.0073 | 0.0245 | 0.014 | 0.0072 | 0.0191 | 0.0288 |
Nucleus | 0.7914 | 0.8985 | 0.8776 | 0.9487 | 0.8635 | 0.903 | 0.7416 | 0.9079 | 0.846 | 0.9135 | 0.844 | 0.9064 | 0.7922 | 0.817 | 0.8738 | 0.9901 | 0.6303 | 0.892 |
Peroxisomes | 0.0139 | 0.0001 | 0.003 | 0 | 0.0001 | 0.0024 | 0.0119 | 0.0003 | 0.0145 | 0.0262 | 0.0002 | 0.0007 | 0.0344 | 0.0124 | 0.0042 | 0 | 0.0261 | 0.0067 |
Punctate Nuclear | 0.0217 | 0.0206 | 0.0286 | 0.0065 | 0.032 | 0.0175 | 0.007 | 0.0238 | 0.0301 | 0.0013 | 0.0149 | 0.0415 | 0.0205 | 0.0251 | 0.0314 | 0.0011 | 0.0169 | 0.0338 |
Vacuole | 0.0035 | 0.0015 | 0.0004 | 0.0002 | 0.016 | 0.0008 | 0.0169 | 0.0006 | 0.0005 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0.0005 | 0.0004 | 0 | 0.0012 | 0.0006 |
Vacuole Periphery | 0.0009 | 0.0003 | 0.0003 | 0.0003 | 0.0059 | 0.0002 | 0.0049 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0.0002 | 0.0011 | 0 | 0.0005 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 69.2303 | 66.8386 | 70.6384 | 68.6206 | 57.4464 | 50.573 | 63.3709 | 59.127 | 58.3206 | 61.781 |
Translational Efficiency | 0.6986 | 0.6561 | 0.6296 | 0.5688 | 0.562 | 0.6101 | 0.5524 | 0.4529 | 0.4496 | 0.4809 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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