ORF | |
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Human Ortholog | |
Description | Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 3 |
Bud | 0 | 2 | 0 | 0 | 0 | 2 | 0 | – | 2 | 1 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 2 | 1 | 4 | 1 | 3 | – | 0 | 4 | 1 | 1 | 0 | 3 | 1 | 3 | 6 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 167 | 318 | 243 | 175 | 241 | 171 | 174 | – | 140 | 89 | 76 | 69 | 297 | 394 | 294 | 96 | 102 | 149 | 164 | 305 | 232 | 209 | 314 | 280 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 1 | 2 | 1 | – | 4 | 1 | 0 | 6 | 0 | 0 | 0 | 3 | 3 | 2 | 0 | 1 | 2 | 1 | 1 | 3 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 2 | – | 5 | 5 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 3 | 3 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 3 | 0 | 1 | 2 | 6 |
Mitochondria | 0 | 3 | 0 | 4 | 3 | 36 | 20 | – | 91 | 65 | 79 | 84 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 1 | 1 | 3 | 4 | 4 |
Nucleus | 0 | 1 | 4 | 3 | 1 | 6 | 4 | – | 4 | 3 | 7 | 12 | 3 | 2 | 4 | 2 | 1 | 3 | 1 | 2 | 1 | 1 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 |
Vac/Vac Membrane | 5 | 0 | 5 | 6 | 22 | 15 | 63 | – | 40 | 39 | 40 | 24 | 0 | 2 | 3 | 3 | 8 | 3 | 2 | 4 | 13 | 5 | 4 | 2 |
Unique Cell Count | 170 | 323 | 249 | 182 | 260 | 208 | 240 | 238 | 178 | 173 | 176 | 300 | 398 | 299 | 101 | 110 | 153 | 174 | 329 | 262 | 234 | 346 | 318 | |
Labelled Cell Count | 173 | 326 | 255 | 189 | 272 | 233 | 268 | 287 | 209 | 208 | 204 | 302 | 402 | 303 | 110 | 122 | 166 | 174 | 329 | 262 | 234 | 346 | 318 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.3 | 8.9 | 8.8 | 8.1 | 9.1 | 6.0 | 7.3 | – | 6.3 | 6.6 | 5.8 | 6.2 | 10.0 | 9.9 | 10.9 | 13.1 | 14.4 | 12.5 | 8.1 | 8.9 | 9.1 |
Std Deviation (1e-4) | 1.3 | 1.2 | 1.4 | 1.5 | 1.7 | 1.4 | 1.4 | – | 1.3 | 1.3 | 1.0 | 1.3 | 1.3 | 1.7 | 1.6 | 3.1 | 2.9 | 2.5 | 1.4 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.05 | -0.55 | -0.26 | – | -0.47 | -0.41 | -0.59 | -0.49 | 0.19 | 0.17 | 0.31 | 0.58 | 0.72 | 0.51 | -0.11 | 0.03 | 0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.9 | -2.6 | -5.6 | -7.8 | – | -10.4 | -11.7 | -12.6 | -13.4 | 1.3 | 1.4 | 0.6 | -1.2 | -2.2 | -0.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 6.8 | 4.7 | – | 10.8 | 10.4 | 11.8 | 12.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.2 | 2.7 | 7.7 | – | 5.6 | 6.7 | 6.9 | 4.7 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.4231 | 3.6685 | 3.0487 | 2.7997 | 2.1644 | 3.0127 | 4.3907 | 4.8814 | 4.5068 | 4.0466 | 3.5423 | 4.2058 | 7.7695 | 9.2741 | 8.0364 | 8.211 | 7.9423 | 8.046 |
Actin | 0.0314 | 0.0011 | 0.0041 | 0.0087 | 0.0128 | 0.0144 | 0.0724 | 0.012 | 0.024 | 0.0011 | 0.0082 | 0.0014 | 0.0144 | 0.0008 | 0.0057 | 0.0049 | 0.0065 | 0.0023 |
Bud | 0.0059 | 0.0008 | 0.0003 | 0.0005 | 0.0004 | 0.0008 | 0.0011 | 0.0002 | 0.0029 | 0.0002 | 0.0008 | 0.0001 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0008 |
Bud Neck | 0.0079 | 0.0002 | 0.0004 | 0.0008 | 0.0009 | 0.0017 | 0.0031 | 0.0002 | 0.0141 | 0.0004 | 0.0014 | 0.001 | 0.0012 | 0.0001 | 0.0003 | 0.0005 | 0.0003 | 0.0022 |
Bud Periphery | 0.0016 | 0.0001 | 0.0001 | 0.0008 | 0.0004 | 0.0006 | 0.0013 | 0.0001 | 0.0035 | 0.0001 | 0.0006 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0 | 0.0008 |
Bud Site | 0.0032 | 0.0077 | 0.0004 | 0.0008 | 0.0014 | 0.0002 | 0.0073 | 0.0012 | 0.0124 | 0.0016 | 0.0014 | 0 | 0.0061 | 0.0006 | 0.0003 | 0.0002 | 0.0003 | 0.0002 |
Cell Periphery | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0008 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.8035 | 0.9564 | 0.9343 | 0.9131 | 0.8218 | 0.9186 | 0.6461 | 0.9283 | 0.8366 | 0.8354 | 0.8689 | 0.9633 | 0.8429 | 0.9775 | 0.9192 | 0.8159 | 0.9399 | 0.9433 |
Cytoplasmic Foci | 0.0261 | 0.0067 | 0.0087 | 0.0083 | 0.0203 | 0.0172 | 0.0772 | 0.0082 | 0.0232 | 0.0812 | 0.0351 | 0.0082 | 0.0088 | 0.0063 | 0.0155 | 0.0189 | 0.0064 | 0.009 |
Eisosomes | 0.0005 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0083 | 0.0016 | 0.0049 | 0.0034 | 0.0052 | 0.0031 | 0.006 | 0.0055 | 0.0084 | 0.0034 | 0.0082 | 0.0035 | 0.0078 | 0.0011 | 0.0025 | 0.0008 | 0.0077 | 0.003 |
Endosome | 0.0353 | 0.0037 | 0.0078 | 0.0236 | 0.0446 | 0.0137 | 0.0524 | 0.0025 | 0.0154 | 0.0503 | 0.0171 | 0.0031 | 0.0143 | 0.0027 | 0.0095 | 0.0064 | 0.0037 | 0.0061 |
Golgi | 0.006 | 0.0003 | 0.001 | 0.0027 | 0.0114 | 0.0017 | 0.0124 | 0.0012 | 0.0119 | 0.0063 | 0.0339 | 0.0002 | 0.0025 | 0.0004 | 0.0016 | 0.0019 | 0.0006 | 0.0005 |
Lipid Particles | 0.0062 | 0 | 0.0025 | 0.001 | 0.008 | 0.0009 | 0.0134 | 0.0001 | 0.0095 | 0.0012 | 0.0009 | 0.0002 | 0.0087 | 0.0001 | 0.0019 | 0.0147 | 0.0003 | 0.0001 |
Mitochondria | 0.003 | 0.0003 | 0.0062 | 0.0106 | 0.0081 | 0.0009 | 0.0225 | 0.0006 | 0.0088 | 0.0012 | 0.0054 | 0.0001 | 0.0025 | 0.0001 | 0.0018 | 0.0005 | 0.0002 | 0.0006 |
None | 0.0254 | 0.0149 | 0.0171 | 0.0074 | 0.0223 | 0.0058 | 0.0177 | 0.0332 | 0.0122 | 0.0067 | 0.0022 | 0.0107 | 0.0443 | 0.0074 | 0.0261 | 0.0903 | 0.03 | 0.0244 |
Nuclear Periphery | 0.0095 | 0.0008 | 0.0022 | 0.0053 | 0.0064 | 0.0028 | 0.0271 | 0.0008 | 0.0019 | 0.0009 | 0.0025 | 0.0012 | 0.0165 | 0.0004 | 0.0014 | 0.0024 | 0.001 | 0.0007 |
Nucleolus | 0.003 | 0.0001 | 0.0002 | 0.0002 | 0.0018 | 0.0001 | 0.0014 | 0 | 0.0005 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.001 | 0 | 0 |
Nucleus | 0.009 | 0.0028 | 0.005 | 0.0071 | 0.0077 | 0.0116 | 0.0076 | 0.0021 | 0.0025 | 0.0014 | 0.0037 | 0.0046 | 0.0165 | 0.0015 | 0.0033 | 0.0022 | 0.0017 | 0.0042 |
Peroxisomes | 0.0024 | 0.0002 | 0.0011 | 0.0003 | 0.003 | 0.0006 | 0.0122 | 0.0026 | 0.0074 | 0.0055 | 0.0083 | 0.0001 | 0.0067 | 0.0002 | 0.0052 | 0.0071 | 0.0001 | 0.0008 |
Punctate Nuclear | 0.0064 | 0.0007 | 0.0017 | 0.0006 | 0.0197 | 0.0032 | 0.0115 | 0.0007 | 0.0009 | 0.0018 | 0.0004 | 0.001 | 0.0022 | 0.0003 | 0.0044 | 0.0314 | 0.0004 | 0.0004 |
Vacuole | 0.0038 | 0.0011 | 0.0015 | 0.0028 | 0.0025 | 0.0015 | 0.0034 | 0.0004 | 0.0023 | 0.001 | 0.0007 | 0.0011 | 0.0018 | 0.0002 | 0.0005 | 0.0003 | 0.0005 | 0.0005 |
Vacuole Periphery | 0.0012 | 0.0002 | 0.0004 | 0.0017 | 0.0009 | 0.0005 | 0.0028 | 0.0001 | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 31.07 | 28.5253 | 23.3951 | 27.2347 | 35.9286 | 30.2135 | 21.6315 | 24.0768 | 21.1248 | 31.6974 |
Translational Efficiency | 1.6954 | 1.2233 | 1.3296 | 1.197 | 1.1362 | 2.1697 | 1.8504 | 1.5408 | 1.9916 | 1.4936 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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