Standard name | |
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Human Ortholog | |
Description | Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase; complex catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 3 | 6 | 0 | 6 | 1 | 6 | 4 | 0 | 0 | 1 | 3 | 3 | 3 | 4 | 1 | 5 | 1 | 1 | 5 | 2 | 1 | 13 | 15 |
Bud | 6 | 1 | 8 | 8 | 3 | 3 | 14 | 11 | 7 | 13 | 17 | 13 | 10 | 3 | 8 | 16 | 14 | 9 | 1 | 0 | 1 | 1 | 4 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 4 | 0 | 2 | 0 | 0 | 1 | 4 | 0 | 5 | 2 | 2 | 0 | 0 | 1 | 0 | 6 | 0 | 2 | – | – | – | – | – | – |
Cell Periphery | 3 | 4 | 1 | 3 | 4 | 3 | 3 | 4 | 1 | 2 | 2 | 2 | 5 | 2 | 4 | 13 | 10 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 2 | 3 | 6 | 13 | 12 | 16 | 12 | 13 | 1 | 7 | 3 | 0 | 9 | 7 | 4 | 9 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 2 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 8 | 4 | 4 | 0 | 0 | 0 | 0 | 1 | 2 |
Endosome | 1 | 4 | 9 | 4 | 11 | 1 | 3 | 2 | 1 | 4 | 0 | 4 | 34 | 30 | 24 | 33 | 32 | 37 | 7 | 5 | 6 | 5 | 24 | 20 |
Golgi | 0 | 7 | 14 | 7 | 47 | 6 | 2 | 5 | 1 | 2 | 2 | 9 | 12 | 25 | 21 | 40 | 25 | 6 | 9 | 12 | 7 | 9 | 20 | 27 |
Mitochondria | 153 | 145 | 122 | 133 | 205 | 203 | 325 | 295 | 295 | 369 | 231 | 312 | 135 | 101 | 95 | 30 | 12 | 12 | 121 | 131 | 127 | 93 | 167 | 131 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 2 | 3 | 2 | 1 | 0 | 0 | 0 | 7 | 6 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 5 | 9 | 7 | 5 | 7 | 11 | 15 | 17 | 20 | 18 | 11 | 7 | 6 | 1 | 0 | 4 | 6 | 0 | 1 | 1 | 0 | 0 | 3 | 4 |
Nucleolus | 13 | 2 | 8 | 5 | 18 | 14 | 49 | 36 | 45 | 46 | 31 | 29 | 17 | 12 | 18 | 14 | 11 | 16 | 0 | 0 | 0 | 1 | 2 | 3 |
Peroxisomes | 18 | 7 | 21 | 18 | 6 | 6 | 11 | 9 | 7 | 13 | 4 | 7 | 53 | 109 | 147 | 77 | 71 | 96 | 17 | 5 | 17 | 17 | 44 | 43 |
SpindlePole | 9 | 0 | 4 | 6 | 2 | 8 | 5 | 4 | 5 | 13 | 1 | 5 | 22 | 49 | 61 | 51 | 34 | 37 | 4 | 0 | 0 | 3 | 9 | 7 |
Vac/Vac Membrane | 9 | 14 | 8 | 8 | 26 | 16 | 5 | 16 | 8 | 29 | 4 | 19 | 14 | 10 | 14 | 47 | 46 | 23 | 1 | 3 | 0 | 2 | 3 | 3 |
Unique Cell Count | 168 | 160 | 151 | 165 | 267 | 236 | 376 | 335 | 336 | 418 | 255 | 352 | 198 | 238 | 290 | 255 | 213 | 209 | 172 | 169 | 167 | 141 | 307 | 267 |
Labelled Cell Count | 224 | 198 | 213 | 203 | 350 | 287 | 463 | 416 | 410 | 516 | 316 | 414 | 311 | 355 | 404 | 351 | 288 | 267 | 172 | 169 | 167 | 141 | 307 | 267 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 11.7 | 9.3 | 10.9 | 16.7 | 15.2 | 14.1 | 15.3 | 12.9 | 13.2 | 13.8 | 15.6 | 9.7 | 12.1 | 13.2 | 10.2 | 11.5 | 10.7 | 9.3 | 9.7 | 11.3 |
Std Deviation (1e-4) | 1.7 | 2.9 | 2.2 | 3.5 | 4.9 | 6.0 | 6.3 | 6.5 | 6.2 | 6.9 | 8.6 | 9.3 | 2.4 | 4.2 | 4.7 | 3.9 | 7.2 | 5.9 | 2.7 | 3.2 | 3.6 |
Intensity Change (Log2) | – | – | – | 0.22 | 0.85 | 0.71 | 0.6 | 0.72 | 0.47 | 0.5 | 0.57 | 0.75 | 0.06 | 0.38 | 0.5 | 0.13 | 0.3 | 0.2 | -0.01 | 0.07 | 0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -0.2 | 0 | 0 | -0.8 | -1.1 | 0 | -1.2 | 0.6 | -0.8 | -0.1 | 0 | -1.4 | 0.4 | 0.5 | -0.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 |
Cytoplasm | 0 | 1.5 | 1.5 | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.6 | -0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 2.1 | 0.9 | 2.2 | 2.7 | 3.3 |
Golgi | -1.8 | 2.3 | -2.9 | 0 | -4.1 | 0 | 0 | -4.2 | -3.3 | -1.1 | 0.4 | -0.7 | 1.8 | 0.7 | -2.6 |
Mitochondria | 0 | -1.0 | 1.4 | 1.6 | 2.1 | 2.0 | 2.3 | 2.8 | 2.3 | -2.6 | -7.5 | -9.6 | -13.9 | -14.6 | -14.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -0.7 | -1.1 | 0 | -0.3 | 0.2 | 0.6 | -0.2 | -0.2 | 0 | -0.8 | 0 | 0 | 0 | -0.9 | 0 |
Nucleolus | -1.0 | 0.6 | 0.3 | 2.6 | 1.9 | 2.7 | 2.1 | 2.3 | 1.2 | 1.2 | -0.1 | 0.4 | 0.1 | -0.1 | 0.9 |
Peroxisomes | -0.8 | -4.7 | -4.3 | -4.8 | -4.8 | -5.2 | -4.8 | -5.0 | -5.3 | 2.9 | 6.5 | 7.5 | 3.7 | 4.2 | 6.4 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 5.0 | 5.2 | 4.9 | 4.1 | 4.4 |
Vacuole | -0.2 | 1.6 | 0.6 | 0 | -0.2 | 0 | 0.7 | 0 | 0 | 0.7 | -0.5 | -0.2 | 3.7 | 4.3 | 1.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 57.8071 | 59.8858 | 55.1237 | 51.4475 | 46.8292 | 54.9988 | 52.1905 | 52.7715 | 46.0093 | 45.4174 | 41.7743 | 48.704 | 49.4671 | 53.1279 | 51.7959 | 51.4988 | 43.3001 | 51.7125 |
Actin | 0.0021 | 0.0015 | 0.0008 | 0.0001 | 0.0006 | 0.0003 | 0.012 | 0.0065 | 0.0041 | 0.0011 | 0.0002 | 0.0003 | 0.0171 | 0.0028 | 0.008 | 0.0273 | 0.0007 | 0.0007 |
Bud | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 |
Bud Neck | 0 | 0 | 0.001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0007 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 |
Bud Periphery | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0016 | 0.0002 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Bud Site | 0.0001 | 0 | 0.0002 | 0 | 0.0009 | 0 | 0.0005 | 0.0001 | 0.0022 | 0.0007 | 0.0002 | 0 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 |
Cell Periphery | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0003 | 0.0006 | 0.0007 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0002 | 0 | 0 | 0.0001 | 0.0024 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0024 | 0 | 0.0068 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0087 | 0.0002 | 0.0049 | 0.0032 | 0.0026 | 0.0007 | 0.0039 | 0.0003 | 0.0063 | 0.0021 | 0.0442 | 0.0002 | 0.0136 | 0.0001 | 0.0036 | 0.0011 | 0.0104 | 0.0001 |
Eisosomes | 0.0013 | 0.0011 | 0.0039 | 0.0002 | 0.0009 | 0.0002 | 0.0011 | 0.0009 | 0.002 | 0.0006 | 0.0002 | 0.0002 | 0.001 | 0.0012 | 0.0012 | 0.0004 | 0.0003 | 0.0009 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0005 | 0 | 0.0026 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Endosome | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0019 | 0.0001 | 0.0039 | 0.0003 | 0.0068 | 0.0003 | 0.0026 | 0.0003 | 0.0091 | 0.0002 | 0.0003 | 0.0008 | 0.0008 | 0.0001 |
Golgi | 0.0013 | 0.0028 | 0.0008 | 0.0003 | 0.0002 | 0.0004 | 0.0061 | 0.0019 | 0.0032 | 0.001 | 0.0008 | 0.0009 | 0.0061 | 0.0013 | 0.001 | 0.0039 | 0.0007 | 0.0014 |
Lipid Particles | 0.0092 | 0.0116 | 0.0051 | 0.0084 | 0.0033 | 0.0049 | 0.0105 | 0.0072 | 0.0163 | 0.0074 | 0.0138 | 0.0074 | 0.0161 | 0.0126 | 0.0052 | 0.0053 | 0.0089 | 0.0099 |
Mitochondria | 0.9598 | 0.9532 | 0.9695 | 0.9799 | 0.9535 | 0.9792 | 0.9391 | 0.9481 | 0.9193 | 0.9698 | 0.9104 | 0.9673 | 0.8961 | 0.9691 | 0.9648 | 0.9572 | 0.9484 | 0.9798 |
None | 0.0004 | 0 | 0.0002 | 0.0004 | 0.0006 | 0 | 0.0002 | 0 | 0.0004 | 0.0002 | 0.0068 | 0 | 0.0023 | 0 | 0.0002 | 0 | 0.0002 | 0 |
Nuclear Periphery | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0121 | 0 | 0.0006 | 0 | 0.0007 | 0.0001 | 0.0015 | 0.0001 | 0.0014 | 0 | 0.0002 | 0 | 0.0003 | 0 |
Nucleolus | 0.0041 | 0.0082 | 0.0021 | 0.0034 | 0.0023 | 0.0031 | 0.0026 | 0.0033 | 0.0019 | 0.0024 | 0.0025 | 0.0001 | 0.0098 | 0.0002 | 0.0011 | 0 | 0.0003 | 0.0001 |
Nucleus | 0.0008 | 0.0006 | 0.0003 | 0.0003 | 0.0086 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0012 | 0 | 0.0009 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Peroxisomes | 0.0066 | 0.0027 | 0.0022 | 0.0005 | 0.0026 | 0.0005 | 0.005 | 0.0031 | 0.007 | 0.0043 | 0.0014 | 0.0005 | 0.0068 | 0.0014 | 0.0047 | 0.0021 | 0.0029 | 0.0012 |
Punctate Nuclear | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0013 | 0 | 0.0001 | 0 | 0.001 | 0 | 0.009 | 0 | 0.0017 | 0 | 0.0001 | 0.0001 | 0.0243 | 0 |
Vacuole | 0.0005 | 0.0007 | 0.0005 | 0.0004 | 0.0012 | 0.0009 | 0.002 | 0.0054 | 0.007 | 0.0009 | 0.0008 | 0.0012 | 0.0023 | 0.0004 | 0.0006 | 0.0001 | 0.0002 | 0.0002 |
Vacuole Periphery | 0.0037 | 0.0167 | 0.0078 | 0.0026 | 0.0036 | 0.0091 | 0.0109 | 0.0218 | 0.0174 | 0.0082 | 0.0014 | 0.0214 | 0.0044 | 0.0104 | 0.0081 | 0.0009 | 0.0011 | 0.0056 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 161.0374 | 154.6211 | 180.0161 | 280.2911 | 246.8128 | 264.2731 | 506.2991 | 519.3989 | 487.4815 | 503.6518 |
Translational Efficiency | 1.6323 | 1.9287 | 1.8908 | 1.3658 | 1.6082 | 2.3657 | 1.8227 | 1.8368 | 1.9227 | 1.8233 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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