Standard name
Human Ortholog
Description Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase; complex catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Bud 0 0 0.05 0.05 0 0 0 0 0 0 0.07 0 0.05 0 0 0.06 0.07 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.06 0 0 0 0 0 0 0 0 0 0.17 0.13 0.08 0.13 0.15 0.18 0 0 0 0 0.08 0.08
Golgi 0 0 0.09 0 0.18 0 0 0 0 0 0 0 0.06 0.11 0.07 0.16 0.12 0 0.06 0.07 0 0.07 0.07 0.1
Mitochondria 0.91 0.91 0.81 0.81 0.77 0.86 0.86 0.88 0.88 0.88 0.91 0.89 0.68 0.42 0.33 0.12 0.06 0.06 0.71 0.78 0.76 0.66 0.55 0.49
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.06 0.05 0 0 0.05 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0 0.05 0 0.07 0.06 0.13 0.11 0.13 0.11 0.12 0.08 0.09 0.05 0.06 0.05 0.05 0.08 0 0 0 0 0 0
Peroxisomes 0.11 0 0.14 0.11 0 0 0 0 0 0 0 0 0.27 0.46 0.51 0.3 0.33 0.46 0.1 0 0.11 0.13 0.15 0.16
SpindlePole 0.05 0 0 0 0 0 0 0 0 0 0 0 0.11 0.21 0.21 0.2 0.16 0.18 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.09 0.05 0.05 0.1 0.07 0 0.05 0 0.07 0 0.05 0.07 0 0.05 0.18 0.22 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 6 0 6 1 6 4 0 0 1 3 3 3 4 1 5 1 1 5 2 1 13 15
Bud 6 1 8 8 3 3 14 11 7 13 17 13 10 3 8 16 14 9 1 0 1 1 4 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 0 0 0 0 0 1 1
Bud Site 4 0 2 0 0 1 4 0 5 2 2 0 0 1 0 6 0 2
Cell Periphery 3 4 1 3 4 3 3 4 1 2 2 2 5 2 4 13 10 9 0 0 0 0 0 0
Cytoplasm 1 2 3 6 13 12 16 12 13 1 7 3 0 9 7 4 9 9 0 0 0 0 0 0
Endoplasmic Reticulum 2 0 0 0 1 1 2 1 0 1 1 0 0 0 0 8 4 4 0 0 0 0 1 2
Endosome 1 4 9 4 11 1 3 2 1 4 0 4 34 30 24 33 32 37 7 5 6 5 24 20
Golgi 0 7 14 7 47 6 2 5 1 2 2 9 12 25 21 40 25 6 9 12 7 9 20 27
Mitochondria 153 145 122 133 205 203 325 295 295 369 231 312 135 101 95 30 12 12 121 131 127 93 167 131
Nucleus 0 0 0 0 1 1 3 0 2 3 2 1 0 0 0 7 6 6 0 0 0 0 0 0
Nuclear Periphery 5 9 7 5 7 11 15 17 20 18 11 7 6 1 0 4 6 0 1 1 0 0 3 4
Nucleolus 13 2 8 5 18 14 49 36 45 46 31 29 17 12 18 14 11 16 0 0 0 1 2 3
Peroxisomes 18 7 21 18 6 6 11 9 7 13 4 7 53 109 147 77 71 96 17 5 17 17 44 43
SpindlePole 9 0 4 6 2 8 5 4 5 13 1 5 22 49 61 51 34 37 4 0 0 3 9 7
Vac/Vac Membrane 9 14 8 8 26 16 5 16 8 29 4 19 14 10 14 47 46 23 1 3 0 2 3 3
Unique Cell Count 168 160 151 165 267 236 376 335 336 418 255 352 198 238 290 255 213 209 172 169 167 141 307 267
Labelled Cell Count 224 198 213 203 350 287 463 416 410 516 316 414 311 355 404 351 288 267 172 169 167 141 307 267


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 11.7 9.3 10.9 16.7 15.2 14.1 15.3 12.9 13.2 13.8 15.6 9.7 12.1 13.2 10.2 11.5 10.7 9.3 9.7 11.3
Std Deviation (1e-4) 1.7 2.9 2.2 3.5 4.9 6.0 6.3 6.5 6.2 6.9 8.6 9.3 2.4 4.2 4.7 3.9 7.2 5.9 2.7 3.2 3.6
Intensity Change (Log2) 0.22 0.85 0.71 0.6 0.72 0.47 0.5 0.57 0.75 0.06 0.38 0.5 0.13 0.3 0.2 -0.01 0.07 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -0.2 0 0 -0.8 -1.1 0 -1.2 0.6 -0.8 -0.1 0 -1.4 0.4 0.5 -0.4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.4 0 0
Cytoplasm 0 1.5 1.5 1.3 0 0 0 0 0 0 0 0 0 0 1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.6 -0.8 0 0 0 0 0 0 0 3.2 2.1 0.9 2.2 2.7 3.3
Golgi -1.8 2.3 -2.9 0 -4.1 0 0 -4.2 -3.3 -1.1 0.4 -0.7 1.8 0.7 -2.6
Mitochondria 0 -1.0 1.4 1.6 2.1 2.0 2.3 2.8 2.3 -2.6 -7.5 -9.6 -13.9 -14.6 -14.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.7 -1.1 0 -0.3 0.2 0.6 -0.2 -0.2 0 -0.8 0 0 0 -0.9 0
Nucleolus -1.0 0.6 0.3 2.6 1.9 2.7 2.1 2.3 1.2 1.2 -0.1 0.4 0.1 -0.1 0.9
Peroxisomes -0.8 -4.7 -4.3 -4.8 -4.8 -5.2 -4.8 -5.0 -5.3 2.9 6.5 7.5 3.7 4.2 6.4
SpindlePole 0 0 0 0 0 0 0 0 0 3.0 5.0 5.2 4.9 4.1 4.4
Vacuole -0.2 1.6 0.6 0 -0.2 0 0.7 0 0 0.7 -0.5 -0.2 3.7 4.3 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 57.8071 59.8858 55.1237 51.4475 46.8292 54.9988 52.1905 52.7715 46.0093 45.4174 41.7743 48.704 49.4671 53.1279 51.7959 51.4988 43.3001 51.7125
Actin 0.0021 0.0015 0.0008 0.0001 0.0006 0.0003 0.012 0.0065 0.0041 0.0011 0.0002 0.0003 0.0171 0.0028 0.008 0.0273 0.0007 0.0007
Bud 0 0 0 0 0.0001 0 0.0001 0.0001 0.0007 0.0001 0.0001 0 0.0002 0 0 0.0001 0 0
Bud Neck 0 0 0.001 0 0 0 0.0002 0 0.0007 0 0 0 0.0003 0 0 0.0001 0 0
Bud Periphery 0.0001 0 0 0 0.0002 0 0.0002 0.0001 0.0016 0.0002 0.0001 0 0.0003 0 0.0001 0.0001 0.0001 0
Bud Site 0.0001 0 0.0002 0 0.0009 0 0.0005 0.0001 0.0022 0.0007 0.0002 0 0.0006 0 0.0001 0.0002 0.0001 0
Cell Periphery 0.0001 0.0002 0.0002 0.0001 0.0008 0.0001 0.0003 0.0006 0.0007 0.0002 0.0001 0.0001 0.0007 0.0001 0.0003 0 0.0001 0.0001
Cytoplasm 0.0002 0 0 0.0001 0.0024 0 0.0001 0 0.0002 0 0.0024 0 0.0068 0 0 0 0.0001 0
Cytoplasmic Foci 0.0087 0.0002 0.0049 0.0032 0.0026 0.0007 0.0039 0.0003 0.0063 0.0021 0.0442 0.0002 0.0136 0.0001 0.0036 0.0011 0.0104 0.0001
Eisosomes 0.0013 0.0011 0.0039 0.0002 0.0009 0.0002 0.0011 0.0009 0.002 0.0006 0.0002 0.0002 0.001 0.0012 0.0012 0.0004 0.0003 0.0009
Endoplasmic Reticulum 0 0 0 0 0.0003 0 0.0002 0 0.0002 0 0.0005 0 0.0026 0 0 0.0001 0.0001 0
Endosome 0.0005 0.0003 0.0002 0.0001 0.0019 0.0001 0.0039 0.0003 0.0068 0.0003 0.0026 0.0003 0.0091 0.0002 0.0003 0.0008 0.0008 0.0001
Golgi 0.0013 0.0028 0.0008 0.0003 0.0002 0.0004 0.0061 0.0019 0.0032 0.001 0.0008 0.0009 0.0061 0.0013 0.001 0.0039 0.0007 0.0014
Lipid Particles 0.0092 0.0116 0.0051 0.0084 0.0033 0.0049 0.0105 0.0072 0.0163 0.0074 0.0138 0.0074 0.0161 0.0126 0.0052 0.0053 0.0089 0.0099
Mitochondria 0.9598 0.9532 0.9695 0.9799 0.9535 0.9792 0.9391 0.9481 0.9193 0.9698 0.9104 0.9673 0.8961 0.9691 0.9648 0.9572 0.9484 0.9798
None 0.0004 0 0.0002 0.0004 0.0006 0 0.0002 0 0.0004 0.0002 0.0068 0 0.0023 0 0.0002 0 0.0002 0
Nuclear Periphery 0.0001 0 0.0002 0.0001 0.0121 0 0.0006 0 0.0007 0.0001 0.0015 0.0001 0.0014 0 0.0002 0 0.0003 0
Nucleolus 0.0041 0.0082 0.0021 0.0034 0.0023 0.0031 0.0026 0.0033 0.0019 0.0024 0.0025 0.0001 0.0098 0.0002 0.0011 0 0.0003 0.0001
Nucleus 0.0008 0.0006 0.0003 0.0003 0.0086 0.0003 0.0003 0.0002 0.0004 0.0002 0.0012 0 0.0009 0 0.0001 0 0.0001 0
Peroxisomes 0.0066 0.0027 0.0022 0.0005 0.0026 0.0005 0.005 0.0031 0.007 0.0043 0.0014 0.0005 0.0068 0.0014 0.0047 0.0021 0.0029 0.0012
Punctate Nuclear 0.0004 0 0.0001 0.0002 0.0013 0 0.0001 0 0.001 0 0.009 0 0.0017 0 0.0001 0.0001 0.0243 0
Vacuole 0.0005 0.0007 0.0005 0.0004 0.0012 0.0009 0.002 0.0054 0.007 0.0009 0.0008 0.0012 0.0023 0.0004 0.0006 0.0001 0.0002 0.0002
Vacuole Periphery 0.0037 0.0167 0.0078 0.0026 0.0036 0.0091 0.0109 0.0218 0.0174 0.0082 0.0014 0.0214 0.0044 0.0104 0.0081 0.0009 0.0011 0.0056

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 161.0374 154.6211 180.0161 280.2911 246.8128 264.2731 506.2991 519.3989 487.4815 503.6518
Translational Efficiency 1.6323 1.9287 1.8908 1.3658 1.6082 2.3657 1.8227 1.8368 1.9227 1.8233

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase; complex catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Idh2

Idh2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Idh2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available