Standard name
Human Ortholog
Description Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; has direct role in INO1 transcriptional memory; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p; premature stop codon (C1430T, Q477-stop) in SK1 background is linked to the aggressively invasive phenotype of SK1 relative to BY4741 (S288C)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0.05 0 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0.06 0.06 – 0.05 0 0 0 0.09 0.09 0.14 0.1 0.15 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.11 – 0.26 0.23 0.27 0.14 0.08 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.94 0.97 0.91 0.91 0.84 0.8 – 0.73 0.73 0.68 0.78 0.83 0.81 0.73 0.81 0.79 0.81 0.92 0.86 0.89 0.7 0.62 0.6
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0.1 0 0.12 0.11 0.12 0.2 – 0.35 0.3 0.32 0.28 0.2 0.19 0.26 0.07 0 0.06 0 0.05 0 0.15 0.19 0.18
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.08 0.1 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Bud 0 0 0 0 1 1 1 – 0 3 3 2 0 0 0 0 0 1 2 4 1 0 4 7
Bud Neck 0 0 1 0 0 0 0 – 2 1 2 4 0 0 0 0 0 0 0 0 1 1 2 2
Bud Site 0 0 1 1 2 2 8 – 13 12 10 23 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 8 5 12 6 10 15 – 12 12 7 3 22 31 52 20 18 15 0 0 1 0 1 0
Endoplasmic Reticulum 0 0 0 0 0 0 2 – 0 0 0 0 0 0 0 6 1 3 0 0 0 2 4 1
Endosome 0 4 0 0 1 0 3 – 1 0 0 0 0 0 1 3 1 0 1 3 2 2 1 8
Golgi 2 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 3 3 1 2 2 1
Mitochondria 7 5 4 8 3 14 30 – 66 75 104 50 20 6 16 8 1 3 0 2 0 4 3 8
Nucleus 210 345 294 204 216 142 211 – 187 238 260 284 211 274 273 170 93 101 212 336 281 204 201 223
Nuclear Periphery 0 2 1 4 2 6 6 – 3 6 6 6 3 4 16 4 0 2 0 1 1 1 0 1
Nucleolus 16 36 6 27 26 21 53 – 88 96 122 101 50 65 99 14 4 7 3 17 11 44 63 64
Peroxisomes 0 1 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 2 2 3 1 2 – 0 3 1 10 0 1 4 0 1 1 0 4 2 2 5 7
Vac/Vac Membrane 0 4 2 0 1 0 1 – 1 2 1 1 2 2 0 8 5 6 4 16 9 22 32 33
Unique Cell Count 223 366 303 224 238 170 264 255 325 385 363 254 340 376 210 118 125 231 393 317 293 328 370
Labelled Cell Count 237 405 316 258 261 197 332 373 448 516 484 308 383 462 233 124 139 231 393 317 293 328 370


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 8.1 7.1 6.7 7.2 5.6 6.1 – 5.2 5.0 5.0 5.7 7.0 6.8 6.5 10.4 10.7 9.8 6.4 6.4 6.6
Std Deviation (1e-4) 1.0 1.8 1.2 1.4 1.5 1.4 2.1 – 1.3 1.0 1.0 1.1 1.6 1.4 1.5 2.8 3.1 2.7 1.2 1.5 1.4
Intensity Change (Log2) – – – -0.08 0.01 -0.34 -0.22 – -0.45 -0.51 -0.51 -0.33 -0.03 -0.07 -0.14 0.55 0.59 0.46 -0.16 -0.15 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 3.6 3.0 0 4.1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.4 0 2.5 2.6 – 2.1 1.6 0.2 0 3.8 4.1 5.7 4.1 5.5 4.6
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.7 0 3.8 5.0 – 8.7 8.2 9.2 5.9 3.8 0 2.2 0 0 0
Nucleus -3.0 -3.1 -5.2 -6.5 – -8.1 -8.3 -9.7 -7.1 -5.6 -6.5 -8.5 -6.1 -6.2 -5.7
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 3.3 0 0 0
Nucleolus 4.7 4.4 4.7 7.0 – 10.2 9.4 9.9 9.0 6.9 6.9 8.7 2.7 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 2.0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8271 3.5026 3.0612 2.8451 2.7282 3.3137 5.2433 5.2092 4.7439 4.2588 4.3008 4.8637 4.5319 4.2769 4.2921 4.5523 4.3442 4.2991
Actin 0.0381 0 0.0001 0 0 0.0037 0.0326 0 0.0041 0.0008 0.0007 0.0001 0.0381 0.0022 0.0026 0.0317 0.0475 0.0059
Bud 0.0003 0 0 0 0 0.0001 0.0011 0.0001 0.0005 0.0003 0.0014 0.0001 0.0009 0 0 0.0001 0.0003 0.0003
Bud Neck 0.0074 0 0 0 0.0001 0.0004 0.0018 0.0001 0.0005 0.0003 0.0011 0.0005 0.0021 0.0003 0 0.0002 0.0013 0.0009
Bud Periphery 0.0003 0 0 0 0 0.0002 0.0013 0.0001 0.0008 0.0012 0.0041 0.0002 0.0016 0 0 0.0002 0.0006 0.0009
Bud Site 0.0034 0 0.0001 0 0.0001 0.0003 0.0121 0.0004 0.0022 0.0008 0.0029 0.0003 0.0042 0.0014 0.0001 0.0004 0.0025 0.0016
Cell Periphery 0.0002 0 0 0 0 0.0001 0.0003 0.0001 0.0001 0.0005 0.0012 0.0001 0.0005 0 0 0 0.0002 0.0002
Cytoplasm 0.0069 0.0001 0.0001 0 0 0 0.0072 0.0009 0.0038 0.0003 0.001 0.0001 0.0116 0.0003 0.0002 0.0149 0.0002 0.0007
Cytoplasmic Foci 0.0066 0 0 0 0 0.0005 0.0159 0.0008 0.0106 0.0005 0.0138 0.0041 0.0095 0.0025 0.0031 0.0096 0.0005 0.0034
Eisosomes 0.0003 0 0 0 0 0.0001 0.0002 0 0.0001 0.0001 0 0 0.0004 0 0 0.0001 0.0006 0.0001
Endoplasmic Reticulum 0.0073 0.0007 0 0 0 0.0001 0.0037 0.0001 0.0019 0.0008 0.0011 0 0.0048 0 0.0008 0.0032 0.0007 0.0019
Endosome 0.0184 0.0004 0.0001 0 0 0.0016 0.0186 0.0017 0.0164 0.0012 0.0466 0.0028 0.0236 0.0002 0.0042 0.006 0.0021 0.0098
Golgi 0.0053 0 0 0 0 0.0022 0.0085 0.0001 0.0047 0.0003 0.0163 0.0009 0.0081 0.0007 0.0011 0.003 0.0024 0.0036
Lipid Particles 0.0047 0 0 0 0 0.0024 0.0389 0.0008 0.0097 0.0037 0.0069 0.001 0.0238 0.0001 0.0071 0.0046 0.0009 0.0107
Mitochondria 0.0027 0.0003 0.0002 0.0001 0.0002 0.0074 0.0114 0.0002 0.0102 0.0138 0.0152 0.0074 0.0165 0.0002 0.001 0.0011 0.0029 0.0072
None 0.0067 0 0.0001 0 0 0.0001 0.0098 0 0.0018 0.0003 0.0003 0 0.0123 0 0.0005 0.001 0.0006 0.0005
Nuclear Periphery 0.0355 0.01 0.0026 0.0003 0.0003 0.0003 0.0236 0.0019 0.0127 0.0143 0.0042 0.0003 0.0225 0.0009 0.0049 0.0018 0.004 0.0084
Nucleolus 0.0599 0.0432 0.051 0.0102 0.1429 0.1094 0.0434 0.0386 0.0289 0.0477 0.1507 0.0769 0.0239 0.0323 0.0206 0.0104 0.1879 0.0584
Nucleus 0.7612 0.9437 0.9436 0.9893 0.8537 0.8697 0.7224 0.9507 0.8764 0.9048 0.6916 0.901 0.7766 0.9567 0.9489 0.9031 0.7416 0.8749
Peroxisomes 0.006 0 0 0 0 0.0009 0.0071 0 0.0046 0.0003 0.0098 0.0015 0.0022 0.0014 0.0037 0.0049 0.0004 0.0046
Punctate Nuclear 0.0262 0.0003 0.0019 0 0.0025 0.0003 0.0358 0.0007 0.0062 0.0015 0.0011 0.0007 0.008 0.0004 0.0005 0.0036 0.0017 0.0027
Vacuole 0.0012 0.0004 0.0001 0 0 0.0002 0.0021 0.0023 0.0021 0.0035 0.0198 0.0017 0.0051 0.0003 0.0002 0.0001 0.0004 0.0022
Vacuole Periphery 0.0014 0.0008 0.0001 0 0 0.0002 0.0022 0.0003 0.0017 0.003 0.0099 0.0004 0.0036 0.0001 0.0004 0.0001 0.0005 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.2281 16.7182 16.859 18.9344 14.6108 18.6914 19.396 17.991 14.8884 19.8346
Translational Efficiency 1.1529 0.9574 0.9999 0.7155 1.0224 0.6005 0.6619 0.8458 0.8471 0.8148

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; has direct role in INO1 transcriptional memory; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p; premature stop codon (C1430T, Q477-stop) in SK1 background is linked to the aggressively invasive phenotype of SK1 relative to BY4741 (S288C)
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Sfl1

Sfl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sfl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available