Standard name
Human Ortholog
Description Alpha subunit of succinyl-CoA ligase; succinyl-CoA ligase is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Bud 0 0 – 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 – 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.06 – 0.05 0.05 0 0 0 0 0 0 0 0.1 0.18 0.23 0.07 0.08 0 0.08 0.06 0.08 0.09 0.11 0.11
Golgi 0 0 – 0 0.1 0 0 0 0 0 0 0 0.09 0.12 0.1 0.21 0.36 0.22 0 0.06 0 0 0 0.07
Mitochondria 0.9 0.83 – 0.76 0.79 0.82 0.86 0.84 0.9 0.91 0.92 0.88 0.79 0.55 0.52 0.12 0.05 0.11 0.71 0.61 0.7 0.65 0.56 0.54
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0.08 0.06 0.08 0.08 0.09 0.12 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0.09 0.1 0.1 0.06 0.07 0.13 0.08 0 0 0 0.12 0.08 0.09 0 0 0 0 0 0
Peroxisomes 0.1 0.1 – 0.12 0.05 0 0 0 0 0 0 0 0.11 0.3 0.34 0.22 0.09 0.2 0.11 0.1 0.06 0.11 0.13 0.11
SpindlePole 0.06 0 – 0.07 0 0 0 0 0 0 0 0 0.06 0.16 0.14 0.14 0.09 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.1 – 0.05 0.12 0.11 0.05 0 0.05 0.05 0 0.07 0.08 0.11 0.08 0.2 0.23 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 – 1 2 0 2 1 0 1 1 4 2 0 1 4 5 2 1 4 0 2 5 9
Bud 9 4 – 9 9 5 9 11 6 10 6 6 3 11 8 3 1 2 0 3 0 1 1 3
Bud Neck 1 0 – 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 1 3
Bud Site 2 1 – 0 0 0 7 1 1 2 1 0 0 0 1 1 0 1 – – – – – –
Cell Periphery 3 3 – 1 3 6 4 2 3 4 1 1 5 4 13 5 2 2 0 0 0 0 0 0
Cytoplasm 2 7 – 6 14 10 11 16 10 5 5 6 1 3 4 3 6 5 0 2 0 0 0 1
Endoplasmic Reticulum 0 1 – 0 0 3 1 2 1 3 2 2 0 0 0 0 2 0 0 0 0 0 0 2
Endosome 5 7 – 7 12 6 10 5 0 2 0 3 17 66 91 7 5 2 17 7 2 16 29 39
Golgi 0 0 – 5 25 5 1 2 1 5 0 4 15 44 41 20 24 12 4 7 1 4 11 26
Mitochondria 184 100 – 99 193 211 384 302 297 328 255 268 129 200 207 12 3 6 146 76 25 114 157 197
Nucleus 2 1 – 2 2 3 2 4 2 3 3 2 0 1 0 1 1 2 0 1 0 0 0 1
Nuclear Periphery 4 0 – 2 4 21 26 28 27 33 33 15 6 2 1 5 0 1 0 0 0 1 2 3
Nucleolus 8 3 – 5 5 23 46 37 20 26 36 25 2 5 3 12 5 5 0 0 1 1 2 1
Peroxisomes 20 12 – 16 13 7 13 8 4 4 1 4 18 111 135 21 6 11 22 12 2 19 36 40
SpindlePole 12 4 – 9 4 1 5 6 8 2 1 1 9 57 57 14 6 4 5 5 0 4 11 15
Vac/Vac Membrane 10 12 – 6 28 28 21 10 17 19 7 21 13 41 32 19 15 8 2 2 0 3 12 12
Unique Cell Count 205 121 131 243 257 445 360 331 362 277 304 163 364 398 97 66 55 208 125 36 176 280 363
Labelled Cell Count 262 156 168 314 329 542 435 397 447 352 362 220 546 594 128 82 63 208 125 36 176 280 363


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 7.2 – 6.9 9.4 10.7 10.8 11.5 11.0 11.6 10.8 12.0 8.1 8.2 7.9 14.8 14.3 17.0 6.6 7.4 7.6
Std Deviation (1e-4) 0.9 1.6 – 1.6 1.9 2.5 2.9 3.2 3.1 3.3 3.4 3.5 2.4 1.4 1.5 4.8 5.1 5.2 1.5 1.9 1.6
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.4155 9.6731 8.8687 9.8256 8.0184 9.0994 6.9748 8.9826 8.3848 8.4084 8.7388 8.4852 11.1676 11.366 10.4576 7.8871 8.2465 10.696
Actin 0.016 0.0049 0.0015 0.0101 0.0391 0.0067 0.033 0.0095 0.0027 0.0013 0.0018 0.0003 0.0118 0.0025 0.0104 0.0461 0.0332 0.0032
Bud 0.0003 0.0004 0.0003 0.0002 0.0007 0 0.0004 0.0005 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0015 0.0005 0
Bud Neck 0.0023 0.0002 0.0001 0.0002 0.0007 0 0.0023 0.0002 0.0001 0.0001 0 0.0001 0.0001 0 0.0001 0.0002 0.0042 0.0001
Bud Periphery 0.0005 0.0005 0.0007 0.0001 0.0009 0.0001 0.0006 0.0006 0.0005 0.0001 0.0003 0.0001 0.0003 0.0002 0.0002 0.0043 0.0006 0.0001
Bud Site 0.0014 0.0038 0.0007 0.0016 0.003 0 0.0156 0.0019 0.0005 0.0003 0 0.0001 0.0003 0.0001 0.0005 0.0022 0.0673 0.0001
Cell Periphery 0.0004 0.0003 0.0002 0.0001 0.0002 0.0001 0.0005 0.0001 0.0002 0 0 0.0001 0.0001 0.0001 0.0001 0.0005 0.0002 0.0001
Cytoplasm 0.0031 0.0003 0.0005 0.0034 0.0006 0 0.0008 0 0.0086 0 0 0 0.0003 0 0 0.0023 0.0003 0
Cytoplasmic Foci 0.0055 0.002 0.0031 0.0046 0.0041 0.0003 0.0219 0.0026 0.0062 0.0113 0.0002 0.0004 0.007 0.001 0.0006 0.0116 0.0271 0.0012
Eisosomes 0.0006 0.0002 0.0002 0.0002 0.0004 0.0001 0.0005 0.0002 0.0001 0 0.0001 0.0001 0.0002 0.0002 0.0001 0.0004 0.0002 0.0002
Endoplasmic Reticulum 0.0028 0.0007 0.0004 0.0022 0.0019 0 0.0006 0 0.0027 0 0 0.0002 0.0008 0 0 0.0032 0.0003 0
Endosome 0.0146 0.0112 0.0048 0.0068 0.0124 0.0028 0.0285 0.0065 0.009 0.0078 0.0004 0.0114 0.0102 0.0037 0.0021 0.0365 0.0134 0.0018
Golgi 0.0082 0.0248 0.0052 0.0078 0.0103 0.0044 0.0194 0.029 0.013 0.0085 0.0013 0.0064 0.011 0.0167 0.0094 0.0185 0.0182 0.0055
Lipid Particles 0.022 0.0058 0.0114 0.002 0.0057 0.0067 0.058 0.006 0.0062 0.0528 0.0002 0.012 0.0172 0.0138 0.0016 0.0223 0.0393 0.0053
Mitochondria 0.8706 0.9255 0.948 0.9494 0.8857 0.965 0.7698 0.9204 0.9216 0.881 0.9912 0.95 0.9067 0.9373 0.9704 0.8106 0.7296 0.9781
None 0.0031 0 0.0001 0.0014 0.0009 0 0.0013 0 0.0015 0.0001 0 0 0.0004 0 0 0.0017 0.0004 0
Nuclear Periphery 0.0111 0.0003 0.0012 0.0029 0.0201 0.0002 0.0018 0 0.0012 0.0001 0 0.0038 0.0064 0.0002 0 0.0091 0.0006 0.0001
Nucleolus 0.0014 0.0002 0.0003 0.0003 0.0004 0 0.0045 0.0002 0.0002 0.0001 0 0.0002 0.0002 0.0002 0.0001 0.0005 0.0001 0
Nucleus 0.0053 0.0001 0.0028 0.0014 0.0019 0 0.0012 0 0.0006 0 0 0.0002 0.0005 0 0 0.0019 0.0001 0
Peroxisomes 0.0044 0.0027 0.0049 0.0008 0.0058 0.0008 0.0267 0.0158 0.0153 0.0344 0.0038 0.0007 0.0083 0.003 0.0014 0.0085 0.0603 0.0021
Punctate Nuclear 0.0137 0 0.0001 0.0032 0.0026 0 0.0039 0 0.0004 0.0007 0 0.0001 0.0018 0 0 0.0048 0.0015 0
Vacuole 0.0037 0.005 0.0043 0.0005 0.0007 0.0015 0.0066 0.0015 0.0023 0.0005 0.0001 0.002 0.0022 0.0024 0.0004 0.0075 0.0008 0.0002
Vacuole Periphery 0.0089 0.0111 0.0093 0.0009 0.0019 0.0111 0.0022 0.0047 0.0069 0.0007 0.0004 0.012 0.0139 0.0183 0.0024 0.0058 0.0018 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 103.6531 84.6889 85.0226 111.5476 91.7615 83.545 105.2731 122.7266 123.6881 124.7318
Translational Efficiency 1.8052 1.8859 1.7602 1.5263 1.9546 2.8884 1.9212 1.7971 1.8891 1.8948

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Alpha subunit of succinyl-CoA ligase; succinyl-CoA ligase is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Lsc1

Lsc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lsc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available