Standard name
Human Ortholog
Description Subunit of an alternative replication factor C complex; important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0.13 0.08 0.15 0.17 0.15 0.16 0.11 0.1 0.1 0.12 0.05 0.12 0.14 0.08 0.1 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0.26 0.37 0.15 0.34 0.19 0.45 0.53 0.37 0.68 0.66 0.74 0.53 0.13 0 0.06 0 0 0.1 0 0 0 0 0 0
Nucleus 0.95 0.8 0.78 0.77 0.7 0.6 0.58 0.61 0.56 0.58 0.57 0.63 0.87 0.82 0.8 0.77 0.81 0.86 0.91 0.79 0.72 0.67 0.53 0.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0.1 0.11 0.1 0.06 0.11 0.18 0.23 0.31 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 0
Bud 0 0 2 2 1 0 9 8 11 8 6 13 1 0 1 0 0 0 0 3 1 5 4 4
Bud Neck 0 0 6 0 1 2 4 7 3 3 0 6 0 0 0 0 0 0 0 0 0 0 3 2
Bud Site 0 0 1 1 0 1 7 2 10 12 10 17 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 15 20 10 48 38 59 65 33 28 31 45 12 29 40 10 10 2 0 6 8 2 6 6
Endoplasmic Reticulum 0 3 0 0 0 1 1 0 0 0 0 0 0 2 4 4 2 0 0 0 0 0 1 1
Endosome 0 0 1 1 2 1 2 1 0 0 0 0 0 0 0 1 0 0 0 2 0 0 4 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 1 1 0 0 0 0 1 1
Mitochondria 32 87 23 40 58 102 210 154 213 188 225 200 30 8 17 3 2 2 0 2 1 2 6 7
Nucleus 119 188 124 92 217 136 228 250 176 166 172 238 195 202 223 99 79 18 112 196 129 107 155 146
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 0 2 3 2 0 0 0 4 1 0 0 0 0 0 0 0 3 5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 3 1 6 12 7 4 2 12 0 1 0 0 0 0 0 1 3 2 6 5
Vac/Vac Membrane 1 8 3 2 11 6 7 11 10 3 7 7 12 9 19 13 11 2 7 28 31 36 89 99
Unique Cell Count 125 236 158 119 310 228 395 413 314 286 303 380 224 245 279 128 97 21 123 247 179 160 290 291
Labelled Cell Count 153 301 180 148 341 288 533 512 466 415 453 539 251 255 305 134 105 25 123 247 179 160 290 291


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.0 4.9 4.3 4.9 3.9 3.9 4.4 3.5 3.5 3.2 3.9 4.6 4.9 5.0 7.3 7.4 7.1 4.3 4.5 4.7
Std Deviation (1e-4) 0.5 0.7 1.0 1.1 1.1 1.4 0.8 1.1 1.2 1.1 0.8 1.1 0.7 1.5 1.5 1.6 1.8 1.4 1.1 1.2 1.3
Intensity Change (Log2) – – – -0.18 0.01 -0.32 -0.32 -0.16 -0.46 -0.49 -0.64 -0.35 -0.09 0.0 0.03 0.58 0.6 0.54 -0.18 -0.14 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 3.0 3.2 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7561 1.8657 2.1523 1.7279 2.3425 2.0969 -0.5712 -0.0111 -0.1808 -0.5948 0.1919 -0.3849 -0.4081 0.1296 -0.3524 -0.4879 0.4123 -0.3912
Actin 0.0281 0.0001 0.0013 0.0002 0.0028 0.0148 0.0051 0.0006 0.0119 0.0027 0.0132 0.0008 0.0064 0.001 0.0006 0 0.0002 0.0006
Bud 0.0006 0.0001 0.0008 0 0.0001 0.0032 0.0007 0.0001 0.0003 0.0001 0.0013 0.0003 0.0005 0 0.0001 0.0001 0.0007 0.0001
Bud Neck 0.0124 0.0003 0.0006 0.0005 0.0013 0.0034 0.0047 0.0003 0.0006 0.0002 0.0012 0.0035 0.0026 0.0001 0.0001 0.0012 0.0135 0.0014
Bud Periphery 0.0021 0.0001 0.0014 0.0001 0.0002 0.001 0.0015 0.0001 0.0007 0.0001 0.0037 0.001 0.0008 0 0 0.0001 0.0006 0.0001
Bud Site 0.0086 0.0003 0.0029 0.0001 0.0003 0.0007 0.0037 0.0003 0.0026 0.0002 0.0036 0.0005 0.0051 0.0002 0.0003 0.0001 0.0722 0.0001
Cell Periphery 0.0007 0.0001 0.0007 0 0.0001 0.0001 0.0005 0 0.0003 0 0.0005 0.0002 0.0003 0 0 0 0.0006 0
Cytoplasm 0.0321 0.0237 0.0126 0.0149 0.032 0.0204 0.0105 0.0189 0.0047 0.0041 0.0065 0.0043 0.0294 0.0314 0.0021 0.0026 0.0027 0.0066
Cytoplasmic Foci 0.0079 0.0003 0.0063 0.0004 0.0092 0.0021 0.0071 0.0005 0.0015 0.0021 0.0057 0.0022 0.0138 0.001 0.0062 0 0.0587 0.0035
Eisosomes 0.0005 0.0001 0.0002 0 0.0001 0.0002 0.0001 0 0.0001 0 0.0004 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0048 0.0021 0.0013 0.0013 0.0108 0.001 0.0022 0.0016 0.0012 0.0003 0.0022 0.0003 0.0021 0.0026 0.0001 0.0001 0.0002 0.001
Endosome 0.0077 0.0016 0.0072 0.0015 0.0091 0.0026 0.0115 0.002 0.0038 0.013 0.0548 0.006 0.0105 0.0017 0.0074 0 0.0057 0.005
Golgi 0.0059 0 0.0022 0 0.0037 0.0022 0.003 0 0.0033 0.0168 0.0207 0.0031 0.0025 0.0002 0.0058 0 0.0026 0.0008
Lipid Particles 0.0112 0.0001 0.0079 0.0002 0.0284 0.0022 0.01 0.0002 0.0025 0.0011 0.0182 0.0077 0.0085 0.0006 0.0028 0 0.0057 0.0018
Mitochondria 0.0052 0.0004 0.0105 0.0017 0.0434 0.0013 0.008 0.0005 0.0063 0.0055 0.0703 0.0078 0.0042 0.0003 0.0016 0.0002 0.0003 0.0004
None 0.0855 0.0664 0.0475 0.0224 0.0724 0.0606 0.0086 0.0232 0.0014 0.0241 0.0016 0.0065 0.0268 0.0294 0.0003 0.0001 0.0005 0.0009
Nuclear Periphery 0.0147 0.0166 0.004 0.0257 0.0285 0.0116 0.0244 0.0397 0.0116 0.016 0.0187 0.0061 0.0139 0.0127 0.0036 0.0025 0.0021 0.0073
Nucleolus 0.0097 0.0067 0.0096 0.0054 0.0116 0.0145 0.014 0.0087 0.0043 0.0045 0.0075 0.0086 0.0081 0.0033 0.0019 0.0025 0.0056 0.0058
Nucleus 0.7321 0.8422 0.8573 0.9176 0.7035 0.8332 0.858 0.8877 0.9362 0.9007 0.7237 0.9314 0.8263 0.9108 0.9643 0.9892 0.7714 0.9536
Peroxisomes 0.013 0 0.0131 0.0001 0.0267 0.0014 0.0058 0.0001 0.0011 0.0003 0.0139 0.0026 0.006 0.0001 0.0014 0 0.0077 0.0007
Punctate Nuclear 0.0115 0.0367 0.0101 0.0065 0.013 0.0225 0.0127 0.0145 0.0023 0.0072 0.0045 0.0054 0.0281 0.0034 0.0007 0.0009 0.0422 0.0097
Vacuole 0.0049 0.0018 0.0019 0.001 0.0019 0.0007 0.0057 0.0005 0.002 0.0004 0.0188 0.001 0.0034 0.0009 0.0004 0.0003 0.0065 0.0004
Vacuole Periphery 0.0008 0.0005 0.0006 0.0004 0.0011 0.0003 0.0021 0.0004 0.0013 0.0005 0.009 0.0006 0.0006 0.0002 0.0001 0 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.5944 4.9817 5.545 12.4957 15.3538 15.7807 8.4999 6.7993 9.7602 16.3939
Translational Efficiency 0.5278 0.6854 0.6774 0.5538 0.578 0.7977 0.3994 0.475 0.5186 0.5355

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of an alternative replication factor C complex; important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication
Localization
Cell Percentages nucleus (82%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Elg1

Elg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Elg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available