Standard name | |
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Human Ortholog | |
Description | RNA polymerase II second largest subunit B150; part of central core; similar to bacterial beta subunit |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | – | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 5 |
Bud Neck | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 0 | 1 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 4 | 5 | – | 7 | 15 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 19 | 13 | 5 | 22 | 11 | 22 | 32 | 37 | 29 | – | 16 | 26 | 6 | 16 | 21 | 7 | 0 | 7 | 2 | 0 | 0 | 0 | 1 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 0 |
Golgi | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 6 | 12 | 11 | 11 | 2 | 23 | 35 | 22 | 50 | – | 31 | 48 | 0 | 1 | 4 | 4 | 0 | 3 | 0 | 0 | 2 | 0 | 2 | 2 |
Nucleus | 262 | 125 | 221 | 157 | 247 | 247 | 352 | 533 | 364 | – | 248 | 369 | 209 | 186 | 194 | 68 | 14 | 34 | 226 | 123 | 175 | 92 | 134 | 141 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | – | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 194 | 104 | 152 | 75 | 109 | 145 | 269 | 417 | 340 | – | 250 | 380 | 176 | 115 | 141 | 43 | 5 | 22 | 128 | 52 | 107 | 66 | 91 | 128 |
Peroxisomes | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 3 |
Vac/Vac Membrane | 7 | 1 | 1 | 1 | 2 | 4 | 2 | 11 | 5 | – | 2 | 6 | 1 | 2 | 7 | 8 | 0 | 4 | 12 | 7 | 9 | 10 | 18 | 21 |
Unique Cell Count | 366 | 177 | 286 | 210 | 306 | 363 | 532 | 812 | 578 | 398 | 609 | 262 | 240 | 270 | 107 | 16 | 59 | 377 | 187 | 304 | 175 | 257 | 309 | |
Labelled Cell Count | 489 | 259 | 394 | 269 | 372 | 448 | 699 | 1029 | 796 | 559 | 851 | 393 | 320 | 372 | 132 | 19 | 70 | 377 | 187 | 304 | 175 | 257 | 309 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 45.5 | 40.0 | 22.9 | 21.0 | 23.9 | 21.5 | 21.3 | 20.9 | 18.9 | – | 17.5 | 17.1 | 32.6 | 30.1 | 25.8 | 27.6 | 46.8 | 29.8 | 32.7 | 28.3 | 32.9 |
Std Deviation (1e-4) | 17.8 | 17.4 | 6.7 | 7.5 | 6.8 | 7.0 | 6.5 | 6.2 | 5.9 | – | 5.7 | 5.5 | 9.1 | 9.6 | 9.4 | 11.8 | 12.4 | 13.9 | 11.4 | 10.7 | 11.9 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.06 | -0.09 | -0.1 | -0.13 | -0.27 | – | -0.39 | -0.42 | 0.51 | 0.4 | 0.18 | 0.27 | 1.03 | 0.38 | 0.52 | 0.31 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4.2 | 1.4 | 2.7 | 2.8 | 2.1 | 2.3 | – | 1.7 | 1.9 | 0.5 | 2.9 | 3.4 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.7 | -2.6 | 1.4 | 1.6 | -1.0 | 2.6 | – | 2.1 | 2.3 | -3.2 | -2.6 | -1.7 | 0 | 0 | 0 |
Nucleus | -0.6 | 1.0 | -2.6 | -3.3 | -3.6 | -4.2 | – | -4.2 | -4.9 | 0.7 | 0.1 | -1.5 | -2.7 | 1.0 | -3.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -3.9 | -4.3 | -3.4 | -0.7 | -0.5 | 1.6 | – | 2.5 | 2.6 | 3.3 | -1.2 | -0.2 | -2.3 | -1.7 | -2.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 37.2082 | 44.0258 | 38.261 | 37.4065 | 36.7598 | 38.9349 | 26.4047 | 38.6643 | 34.8595 | 33.4076 | 31.6701 | 35.0102 | 25.2761 | 29.5336 | 30.7096 | 30.3242 | 26.44 | 30.7779 |
Actin | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0048 | 0.0013 | 0.0057 | 0.0007 | 0.0036 | 0.0013 | 0.0001 | 0 | 0.0138 | 0 | 0.0001 | 0 |
Bud | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0017 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0.0002 | 0.001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0049 | 0 | 0.0001 | 0.0001 |
Bud Periphery | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0.0002 | 0.0004 | 0.0005 | 0.0006 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0018 | 0.0001 | 0.0007 | 0.0004 | 0.0008 | 0.0001 | 0.0014 | 0.0001 | 0.0121 | 0 | 0.0002 | 0 |
Cell Periphery | 0 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0003 | 0 |
Cytoplasm | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.004 | 0 | 0.002 | 0.0191 | 0.0006 | 0.001 | 0.0018 | 0 | 0.0004 | 0 | 0.0001 | 0 |
Cytoplasmic Foci | 0 | 0 | 0.0016 | 0 | 0 | 0 | 0.0116 | 0.0001 | 0.0046 | 0.001 | 0.0008 | 0.0005 | 0.0205 | 0 | 0.0066 | 0 | 0.0088 | 0 |
Eisosomes | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0.001 | 0 | 0.0006 | 0 | 0.0004 | 0.0002 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0.0079 | 0.0001 | 0.0021 | 0.0006 | 0.0014 | 0.0011 | 0.0262 | 0 | 0.0026 | 0 | 0.0001 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0.0012 | 0.0001 | 0.0008 | 0.0004 | 0.0004 | 0.0003 | 0.0022 | 0 | 0.0028 | 0 | 0.0001 | 0 |
Lipid Particles | 0 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0033 | 0.0001 | 0.0023 | 0.0005 | 0.0004 | 0.0003 | 0.0154 | 0 | 0.0031 | 0 | 0.0035 | 0 |
Mitochondria | 0.0012 | 0.0005 | 0.001 | 0.001 | 0.0128 | 0.0004 | 0.0047 | 0.0062 | 0.0034 | 0.0117 | 0.0049 | 0.0053 | 0.0015 | 0.0004 | 0.0007 | 0.0004 | 0.0069 | 0.0002 |
None | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0029 | 0 | 0.0015 | 0.0002 | 0.0005 | 0.0001 | 0.0012 | 0 | 0.0003 | 0 | 0.0009 | 0 |
Nuclear Periphery | 0 | 0.0002 | 0.0002 | 0.0011 | 0.0007 | 0.0001 | 0.0363 | 0.0004 | 0.0067 | 0.0012 | 0.015 | 0.0058 | 0.0024 | 0.0003 | 0.0003 | 0.0004 | 0.0008 | 0.0001 |
Nucleolus | 0.191 | 0.1236 | 0.1203 | 0.0759 | 0.5121 | 0.302 | 0.1526 | 0.0824 | 0.078 | 0.0907 | 0.3888 | 0.2538 | 0.165 | 0.0906 | 0.0532 | 0.0411 | 0.4899 | 0.2847 |
Nucleus | 0.8075 | 0.8748 | 0.8745 | 0.9208 | 0.4699 | 0.6956 | 0.6768 | 0.9068 | 0.8765 | 0.8691 | 0.5565 | 0.7239 | 0.7364 | 0.9078 | 0.8926 | 0.9576 | 0.4821 | 0.7146 |
Peroxisomes | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0015 | 0.0001 | 0.0005 | 0.0003 | 0.0003 | 0.0001 | 0.0011 | 0 | 0.0046 | 0 | 0.0003 | 0 |
Punctate Nuclear | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0838 | 0.0017 | 0.0122 | 0.0015 | 0.0222 | 0.0036 | 0.021 | 0.0002 | 0.0007 | 0.0001 | 0.0023 | 0.0001 |
Vacuole | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0006 | 0.0003 | 0.0012 | 0.0001 | 0.0006 | 0.0008 | 0.0007 | 0.0005 | 0.0018 | 0.0001 | 0.0004 | 0.0001 | 0.0008 | 0 |
Vacuole Periphery | 0.0001 | 0.0003 | 0.0002 | 0.0006 | 0.0021 | 0.0001 | 0.0015 | 0.0001 | 0.0008 | 0.0007 | 0.0011 | 0.001 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0023 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 127.7547 | 123.4648 | 107.5494 | 138.2441 | 107.8312 | 93.0248 | 118.322 | 120.888 | 113.25 | 135.3627 |
Translational Efficiency | 0.7599 | 0.7421 | 0.7822 | 0.555 | 0.6375 | 0.8399 | 0.7209 | 0.4721 | 0.5338 | 0.4794 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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