Standard name
Human Ortholog
Description Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter actively regulated by Pdr1p; also involved in steroid transport, cation resistance, and cellular detoxification during exponential growth; PDR5 has a paralog, PDR15, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.13 0.15 0.19 0.22 0.42 0.5 0.43 – 0.45 0.4 0.5 0.17 0.09 0.1 0.14 0.14 0.09 0 0 0 0 0 0
Bud 0 0 0 0 0 0.07 0.05 0.08 – 0.08 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.92 0.89 0.93 0.92 0.81 0.73 0.77 0.68 – 0.68 0.71 0.7 0.92 0.93 0.92 0.8 0.74 0.78 0.92 0.93 0.96 0.89 0.92 0.9
Cytoplasm 0.05 0.06 0 0.06 0.15 0.09 0.08 0.15 – 0.14 0.19 0.16 0.08 0.08 0.06 0.15 0.22 0.16 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0.05 0 0 0.05 0 0.07 0.11 – 0.11 0.06 0.09 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.09 0 0.18 0.15 0.09 – 0.19 0.19 0.19 0 0 0 0.1 0.07 0.13 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 18 58 65 47 50 141 178 128 – 172 134 179 32 11 16 67 52 22 6 6 5 4 5 8
Bud 3 3 3 1 1 22 18 24 – 29 28 19 0 0 0 7 11 4 10 4 1 2 2 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 393 386 400 232 182 243 274 205 – 257 241 254 175 116 146 397 273 197 483 525 479 292 223 318
Cytoplasm 20 27 17 14 33 30 28 44 – 52 63 56 15 10 10 72 79 41 2 10 1 1 1 2
Endoplasmic Reticulum 21 22 13 7 11 14 24 33 – 42 21 32 4 0 0 14 9 14 10 6 5 8 4 7
Endosome 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0
Golgi 1 0 0 0 0 0 1 0 – 0 0 1 0 0 0 1 1 2 0 0 0 2 1 0
Mitochondria 1 7 6 23 4 61 54 28 – 74 64 69 0 0 0 51 27 33 2 4 5 5 0 5
Nucleus 0 0 0 0 1 0 2 0 – 1 5 0 0 0 0 3 3 3 0 0 0 2 0 0
Nuclear Periphery 0 0 0 1 2 2 2 1 – 4 1 2 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 1 0 0 – 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 2 0 – 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 – 0 0 2 1 0 1 1 2 1 0 0 0 0 0 0
Vac/Vac Membrane 0 5 0 0 0 3 0 0 – 2 4 8 2 0 0 2 8 1 1 4 0 5 1 3
Unique Cell Count 427 434 432 251 224 334 356 301 380 338 361 191 125 158 494 367 254 523 567 502 328 244 355
Labelled Cell Count 457 508 504 325 285 517 584 464 635 562 622 229 137 173 615 466 319 523 567 502 328 244 355


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 33.1 28.9 26.2 26.9 24.4 21.0 21.2 21.6 – 18.1 17.6 17.0 28.5 29.1 28.9 16.1 17.3 20.3 27.0 27.8 31.1
Std Deviation (1e-4) 7.4 9.4 5.3 5.4 5.9 7.5 5.8 5.4 – 5.2 4.9 4.4 9.7 7.2 6.8 4.3 5.6 6.8 10.6 11.0 14.1
Intensity Change (Log2) – – – 0.04 -0.1 -0.32 -0.31 -0.28 – -0.53 -0.57 -0.63 0.12 0.15 0.14 -0.71 -0.6 -0.37 0.04 0.09 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.3 2.3 8.4 10.6 8.3 – 9.5 7.7 10.5 0.5 -1.8 -1.5 -0.6 -0.3 -2.4
Bud 0 0 4.6 3.8 5.1 – 5.1 5.3 3.9 0 0 0 0 2.5 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 -4.4 -7.4 -6.2 -8.6 – -9.0 -7.9 -8.2 -0.4 0.1 -0.1 -5.4 -7.0 -5.7
Cytoplasm 1.0 4.9 2.9 2.4 5.2 – 5.0 6.6 5.6 2.0 1.9 1.2 5.5 7.6 5.5
Endoplasmic Reticulum -0.2 1.2 0.9 2.5 4.4 – 4.6 2.1 3.5 -0.6 0 0 -0.2 -0.5 1.6
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 4.9 0 8.2 7.3 5.0 – 8.6 8.4 8.5 0 0 0 5.6 4.2 6.3
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.9625 21.9945 19.0932 20.8213 17.1299 20.7018 19.9211 28.9825 25.3768 23.3687 25.5025 26.9006 20.5707 27.2779 23.7769 27.7944 22.7537 25.9026
Actin 0.0143 0.0003 0.0128 0.0064 0.0229 0.0005 0.007 0.0009 0.0039 0.0039 0.0001 0.0036 0.0033 0 0.0193 0 0.0136 0.0045
Bud 0.0035 0.0038 0.0016 0.0012 0.0089 0.0009 0.0015 0.0002 0.0002 0.0004 0.0001 0.0003 0.0021 0 0.0028 0.0001 0.0016 0.0005
Bud Neck 0.0006 0.0004 0.001 0.0008 0.0024 0.0003 0.0006 0.0001 0.0001 0.0003 0 0.0002 0.0061 0.0001 0.0118 0.0023 0.0005 0.0005
Bud Periphery 0.0204 0.008 0.009 0.0103 0.0622 0.0158 0.0147 0.0013 0.0026 0.0037 0.0018 0.0054 0.0128 0.0004 0.0195 0.0021 0.0059 0.0062
Bud Site 0.0037 0.0101 0.0139 0.0047 0.0207 0.0008 0.0087 0.0102 0.0023 0.0024 0.0001 0.0006 0.0143 0.0083 0.0258 0.0048 0.0092 0.0006
Cell Periphery 0.7906 0.8816 0.8516 0.7887 0.711 0.9198 0.8527 0.948 0.9148 0.8832 0.9586 0.9415 0.8146 0.9626 0.8663 0.9564 0.6857 0.9634
Cytoplasm 0.0107 0.0284 0.0179 0.0089 0.0087 0.008 0.0106 0.0054 0.0058 0.0056 0.0015 0.0029 0.0029 0.0096 0.0085 0.006 0.0023 0.0022
Cytoplasmic Foci 0.0022 0.0003 0.0045 0.0085 0.0101 0 0.0084 0 0.0035 0.0117 0 0.0011 0.0072 0 0.0009 0 0.0027 0.0001
Eisosomes 0.0076 0.0016 0.0027 0.0019 0.0102 0.002 0.01 0.004 0.0049 0.0117 0.0013 0.001 0.0056 0.0002 0.0018 0.0001 0.0028 0.0004
Endoplasmic Reticulum 0.0214 0.0159 0.0261 0.0428 0.0344 0.0122 0.0163 0.0099 0.012 0.0151 0.0167 0.0102 0.0067 0.0035 0.0047 0.008 0.0174 0.0051
Endosome 0.0061 0.0002 0.0035 0.018 0.0062 0.0001 0.0053 0 0.001 0.0042 0.0001 0.0017 0.0076 0 0.0039 0 0.0051 0.0001
Golgi 0.0017 0.0001 0.0019 0.0038 0.0019 0 0.0026 0 0.0008 0.001 0 0.0005 0.003 0 0.0038 0 0.0027 0.0002
Lipid Particles 0.0034 0.0006 0.0053 0.0073 0.0067 0.0004 0.0062 0.0003 0.0039 0.0079 0.0002 0.0011 0.0145 0.0001 0.0017 0.0001 0.0076 0.0003
Mitochondria 0.0385 0.0108 0.0094 0.034 0.0262 0.0073 0.0051 0.0005 0.0152 0.0156 0.0012 0.0105 0.006 0.0001 0.0101 0 0.1927 0.001
None 0.0041 0.002 0.0015 0.0017 0.0099 0.0001 0.0041 0.0001 0.0057 0.008 0.001 0.001 0.0012 0 0.0004 0 0.0057 0
Nuclear Periphery 0.0099 0.0026 0.0043 0.008 0.0054 0.0011 0.0045 0.0021 0.0012 0.0021 0.0021 0.0017 0.0095 0.0015 0.001 0.0009 0.009 0.0005
Nucleolus 0.0011 0.0002 0.0004 0.0015 0.0018 0.0001 0.0007 0.0001 0.0001 0.0004 0.0002 0.0002 0.0099 0 0.0006 0 0.0043 0.0001
Nucleus 0.0031 0.0004 0.0008 0.0019 0.0026 0.0002 0.0016 0.0001 0.0002 0.0006 0.0004 0.0003 0.0057 0.0001 0.0008 0.0001 0.006 0.0001
Peroxisomes 0.0013 0 0.0028 0.0015 0.0013 0.0001 0.0107 0 0.0007 0.0009 0 0.0005 0.0023 0 0.0009 0 0.002 0.0001
Punctate Nuclear 0.0028 0 0.0009 0.0018 0.0023 0 0.001 0 0.0003 0.0069 0 0.001 0.0222 0 0.0001 0 0.0016 0
Vacuole 0.0482 0.031 0.0266 0.0421 0.0351 0.0278 0.027 0.0168 0.0205 0.0139 0.0141 0.0144 0.0409 0.0133 0.0147 0.0189 0.0168 0.014
Vacuole Periphery 0.0048 0.0016 0.0017 0.0043 0.0091 0.0025 0.0008 0.0001 0.0004 0.0005 0.0004 0.0003 0.0016 0 0.0008 0.0001 0.0049 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 202.6314 418.3835 486.9876 447.4561 292.5248 306.5747 703.5245 708.0738 613.4584 581.4647
Translational Efficiency 0.7321 0.7463 0.7203 0.6702 0.6956 0.6297 0.633 0.5423 0.5867 0.5136

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter actively regulated by Pdr1p; also involved in steroid transport, cation resistance, and cellular detoxification during exponential growth; PDR5 has a paralog, PDR15, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (63%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-1

Pdr5

Pdr5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pdr5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available