Standard name | |
---|---|
Human Ortholog | |
Description | SUMO E3 ligase; catalyzes sumoylation of Yku70p/80p and Sir4p promoting telomere anchoring to the nuclear envelope and regulating telomerase activity; DNA-bound form catalyzes a DNA-damaged triggered sumoylation wave resulting in multisite modification of several DNA repair proteins, enhancing interactions between these proteins and accelerating repair; sumoylates Cse4p, a prerequisite for STUbL-mediated Ub-dependent degradation; role in telomere length maintenance |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 1 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 4 | 15 | – | 9 | 11 | 0 | 0 | 0 | – | – | – | 3 | 2 | 5 | 2 | 4 | 8 |
Bud Neck | 2 | 0 | 26 | 6 | 12 | 6 | 16 | 16 | 14 | – | 4 | 11 | 0 | 0 | 0 | – | – | – | 1 | 3 | 4 | 2 | 5 | 6 |
Bud Site | 0 | 0 | 0 | 2 | 0 | 2 | 10 | 1 | 7 | – | 2 | 6 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 36 | 30 | 107 | 83 | 95 | 135 | 146 | 179 | 125 | – | 73 | 124 | 28 | 60 | 109 | – | – | – | 25 | 22 | 54 | 8 | 26 | 41 |
Endoplasmic Reticulum | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 3 | 1 | – | – | – | 0 | 1 | 1 | 1 | 2 | 2 |
Endosome | 0 | 9 | 1 | 2 | 3 | 1 | 0 | 0 | 1 | – | 0 | 0 | 1 | 7 | 6 | – | – | – | 5 | 6 | 3 | 0 | 7 | 3 |
Golgi | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | – | – | – | 0 | 1 | 1 | 0 | 1 | 0 |
Mitochondria | 49 | 31 | 13 | 52 | 30 | 165 | 190 | 79 | 249 | – | 225 | 186 | 43 | 12 | 19 | – | – | – | 6 | 6 | 28 | 8 | 21 | 20 |
Nucleus | 187 | 155 | 150 | 119 | 141 | 141 | 188 | 124 | 99 | – | 73 | 91 | 153 | 124 | 106 | – | – | – | 196 | 175 | 208 | 38 | 64 | 38 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | – | 1 | 0 | 0 | 1 | 0 | – | – | – | 0 | 1 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | – | – | – | 3 | 3 | 1 | 1 | 3 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 2 | 10 | 10 | 8 | 7 | 11 | 13 | 7 | – | 5 | 13 | 0 | 2 | 1 | – | – | – | 12 | 6 | 20 | 3 | 20 | 18 |
Vac/Vac Membrane | 25 | 52 | 11 | 7 | 18 | 16 | 13 | 13 | 15 | – | 4 | 9 | 10 | 26 | 45 | – | – | – | 74 | 52 | 161 | 42 | 102 | 108 |
Unique Cell Count | 264 | 253 | 297 | 247 | 280 | 396 | 479 | 380 | 406 | 309 | 368 | 213 | 225 | 273 | 336 | 293 | 520 | 114 | 276 | 263 | ||||
Labelled Cell Count | 303 | 281 | 320 | 282 | 309 | 475 | 580 | 432 | 534 | 398 | 451 | 237 | 235 | 287 | 336 | 293 | 520 | 114 | 276 | 263 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 4.6 | 4.8 | 4.5 | 4.9 | 3.8 | 4.0 | 4.4 | 3.4 | – | 3.1 | 3.7 | 4.4 | 4.5 | 4.3 | – | – | – | 3.9 | 4.1 | 4.5 |
Std Deviation (1e-4) | 0.5 | 1.1 | 0.7 | 1.3 | 1.0 | 0.8 | 0.9 | 1.2 | 0.9 | – | 0.9 | 0.7 | 1.2 | 1.2 | 0.9 | – | – | – | 1.4 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.01 | -0.34 | -0.27 | -0.15 | -0.52 | – | -0.62 | -0.39 | -0.13 | -0.11 | -0.17 | – | – | – | -0.29 | -0.24 | -0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.9809 | -0.6316 | -1.0161 | -1.1588 | -1.678 | -0.8936 | 0.1519 | 0.4861 | 0.4494 | 0.316 | 0.1406 | 0.4235 | -0.2911 | -0.4861 | -0.394 | -0.6293 | -0.333 | -0.4096 |
Actin | 0.0071 | 0.0004 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.012 | 0.0008 | 0.0024 | 0.0058 | 0.0013 | 0.0004 | 0.0077 | 0.0001 | 0.0016 | 0 | 0.0054 | 0.0022 |
Bud | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0.0004 | 0.0007 | 0.0001 | 0.001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 |
Bud Neck | 0.0009 | 0.0005 | 0.0004 | 0.0003 | 0.0016 | 0.0032 | 0.0065 | 0.0009 | 0.0043 | 0.0004 | 0.0015 | 0.0015 | 0.0022 | 0.0003 | 0.0004 | 0.0004 | 0.0012 | 0.0021 |
Bud Periphery | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0.0004 | 0.0007 | 0.0002 | 0.0029 | 0.0001 | 0.0005 | 0 | 0.0004 | 0 | 0.001 | 0.0002 |
Bud Site | 0.0023 | 0.0004 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0034 | 0.0034 | 0.0038 | 0.0002 | 0.0059 | 0.0001 | 0.0006 | 0.0005 | 0.0003 | 0 | 0.001 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0229 | 0.0513 | 0.0292 | 0.0263 | 0.0112 | 0.0197 | 0.0054 | 0.0316 | 0.0094 | 0.0151 | 0.0207 | 0.011 | 0.0118 | 0.0235 | 0.0171 | 0.0051 | 0.046 | 0.0096 |
Cytoplasmic Foci | 0.0054 | 0.002 | 0.0029 | 0.002 | 0.001 | 0.0005 | 0.0033 | 0.001 | 0.0022 | 0.0006 | 0.0113 | 0.0011 | 0.0076 | 0.0005 | 0.0018 | 0.0002 | 0.0092 | 0.0013 |
Eisosomes | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0023 | 0.0024 | 0.0015 | 0.0007 | 0.0008 | 0.0013 | 0.0015 | 0.0007 | 0.0005 | 0.0025 | 0.009 | 0.0006 | 0.001 | 0.0005 | 0.0005 | 0.0001 | 0.0011 | 0.0002 |
Endosome | 0.0093 | 0.0023 | 0.0032 | 0.0011 | 0.001 | 0.0009 | 0.0142 | 0.0011 | 0.0012 | 0.0144 | 0.0316 | 0.0013 | 0.0062 | 0.001 | 0.0039 | 0.0001 | 0.0128 | 0.0015 |
Golgi | 0.0012 | 0.0002 | 0.0004 | 0.0001 | 0 | 0 | 0.006 | 0.0001 | 0.0006 | 0.0034 | 0.0036 | 0.0007 | 0.0018 | 0 | 0.0008 | 0 | 0.005 | 0.001 |
Lipid Particles | 0.0039 | 0.0015 | 0.0031 | 0.0002 | 0.0003 | 0.0002 | 0.0054 | 0.0003 | 0.0015 | 0.0024 | 0.0097 | 0.0066 | 0.0083 | 0.0002 | 0.0016 | 0 | 0.0179 | 0.0039 |
Mitochondria | 0.0014 | 0.0007 | 0.002 | 0.0002 | 0.0007 | 0.0002 | 0.0023 | 0.0004 | 0.0143 | 0.0044 | 0.0236 | 0.0055 | 0.0062 | 0.0003 | 0.001 | 0.0001 | 0.0321 | 0.0011 |
None | 0.0647 | 0.0877 | 0.072 | 0.1226 | 0.0434 | 0.0335 | 0.0098 | 0.0318 | 0.0272 | 0.0543 | 0.0095 | 0.0257 | 0.0269 | 0.0876 | 0.0719 | 0.009 | 0.1035 | 0.0443 |
Nuclear Periphery | 0.0235 | 0.0223 | 0.0172 | 0.0217 | 0.0262 | 0.0188 | 0.0074 | 0.0126 | 0.0076 | 0.0219 | 0.0236 | 0.0081 | 0.0123 | 0.0078 | 0.0087 | 0.0025 | 0.0198 | 0.01 |
Nucleolus | 0.0088 | 0.0101 | 0.0077 | 0.0062 | 0.0086 | 0.0167 | 0.0113 | 0.0063 | 0.0069 | 0.0032 | 0.0186 | 0.0109 | 0.0079 | 0.0082 | 0.0101 | 0.0087 | 0.0106 | 0.0153 |
Nucleus | 0.7842 | 0.7385 | 0.8091 | 0.7283 | 0.868 | 0.8426 | 0.8726 | 0.8829 | 0.8698 | 0.8603 | 0.7884 | 0.8817 | 0.836 | 0.8298 | 0.8455 | 0.9338 | 0.6193 | 0.8324 |
Peroxisomes | 0.0044 | 0.0059 | 0.0079 | 0.0001 | 0 | 0 | 0.0041 | 0.0001 | 0.0024 | 0.0003 | 0.0082 | 0.0019 | 0.0137 | 0 | 0.0012 | 0 | 0.0292 | 0.0027 |
Punctate Nuclear | 0.0551 | 0.0711 | 0.0408 | 0.0897 | 0.0359 | 0.0613 | 0.0312 | 0.0238 | 0.0429 | 0.0078 | 0.0205 | 0.0423 | 0.0445 | 0.0388 | 0.0319 | 0.0399 | 0.0817 | 0.0715 |
Vacuole | 0.0016 | 0.0017 | 0.001 | 0.0002 | 0.0006 | 0.0006 | 0.0019 | 0.0013 | 0.0007 | 0.0007 | 0.0067 | 0.0004 | 0.0028 | 0.0006 | 0.0009 | 0 | 0.0016 | 0.0002 |
Vacuole Periphery | 0.0006 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0019 | 0.002 | 0.0002 | 0.0016 | 0.0001 | 0.0003 | 0 | 0.0012 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.8535 | 5.4271 | 4.6983 | 8.0678 | 6.8874 | 12.1353 | 9.0312 | 9.4133 | 10.4717 | 11.0547 |
Translational Efficiency | 0.761 | 0.8412 | 1.0221 | 0.693 | 0.7936 | 0.6846 | 0.704 | 0.6893 | 0.6603 | 0.6629 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|